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UniProtKB - Q948T9 (RBOHB_SOLTU)
Protein
Respiratory burst oxidase homolog protein B
Gene
RBOHB
Organism
Solanum tuberosum (Potato)
Status
Functioni
Calcium-dependent NADPH oxidase that generates superoxide. Involved in the massive phase II oxidative burst induced by pathogen infection.
1 PublicationMiscellaneous
K252a and staurosporine, two protein kinase inhibitors, completely block the RBOHB induction by fungal elicitor.
Activity regulationi
Inhibited by diphenylene iodinium (DPI).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 211 | CalciumBy similarity | 1 | |
Metal bindingi | 213 | CalciumBy similarity | 1 | |
Metal bindingi | 215 | CalciumBy similarity | 1 | |
Metal bindingi | 217 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 222 | CalciumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 211 – 222 | PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- NAD(P)H oxidase H2O2-forming activity Source: GO_Central
- peroxidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Ligand | Calcium, FAD, Flavoprotein, Metal-binding, NADP |
Protein family/group databases
PeroxiBasei | 4546, StRboh02 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RBOHB |
Organismi | Solanum tuberosum (Potato) |
Taxonomic identifieri | 4113 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: CACAO
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 322 | CytoplasmicSequence analysisAdd BLAST | 322 | |
Transmembranei | 323 – 343 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 344 – 358 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 359 – 379 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 380 – 407 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 408 – 428 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 429 – 463 | ExtracellularSequence analysisAdd BLAST | 35 | |
Transmembranei | 464 – 484 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 485 – 506 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 507 – 527 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 528 – 686 | ExtracellularSequence analysisAdd BLAST | 159 | |
Transmembranei | 687 – 707 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 708 – 867 | CytoplasmicSequence analysisAdd BLAST | 160 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 82 | S → A: Decreased activity. 1 Publication | 1 | |
Mutagenesisi | 89 | S → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 97 | S → A: Decreased activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000313764 | 1 – 867 | Respiratory burst oxidase homolog protein BAdd BLAST | 867 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 82 | Phosphoserine; by CPK1 Publication | 1 | |
Modified residuei | 97 | Phosphoserine; by CPK1 Publication | 1 |
Post-translational modificationi
Phosphorylation at Ser-82 and Ser-97 is required for full activity of RBOHB. Not phosphorylated at Ser-89. Phosphorylation at Ser-82 is induced by fungal elicitor treatment.1 Publication
Keywords - PTMi
PhosphoproteinPTM databases
iPTMneti | Q948T9 |
Expressioni
Inductioni
By fungal elicitor, arachidonic acid and salicylic acid.2 Publications
Gene expression databases
ExpressionAtlasi | Q948T9, baseline |
Interactioni
Subunit structurei
Monomer and homodimer.
By similarityProtein-protein interaction databases
STRINGi | 4113.PGSC0003DMT400063688 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 198 – 233 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 242 – 277 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 361 – 519 | Ferric oxidoreductaseAdd BLAST | 159 | |
Domaini | 558 – 681 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 124 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 141 – 149 | EF-hand-like 1By similarity | 9 | |
Regioni | 175 – 186 | EF-hand-like 2By similarityAdd BLAST | 12 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
InParanoidi | Q948T9 |
OrthoDBi | 936110at2759 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR013623, NADPH_Ox IPR029654, RBOHB IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR11972:SF64, PTHR11972:SF64, 1 hit |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit PF08414, NADPH_Ox, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SMARTi | View protein in SMART SM00054, EFh, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q948T9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEIENTRDSD SMRGSRVGFS GSLVSGKKSA RFKDDESYVE ITLDVRDDSV
60 70 80 90 100
SVQNIKGADH EAALLASRLE KRPNNTLGSQ LSFHLRQVSK ELKRMTSSNK
110 120 130 140 150
FQKIDRSKSG AARALRGLQF MNKNVGTEGW SEVESRFDQL AVNGMLTKSL
160 170 180 190 200
FGQCIGMKES SEFAEELFDA LARKRCITSP AVTKDELREF WEQITDTSFD
210 220 230 240 250
ARLQTFFDMV DKDADGRITQ EEVKEIISLS ASANKLSKIQ DNSDEYAALI
260 270 280 290 300
MEELDPGNVG YIELYNLETL LLQAPSHSMN LSTNSRVLSR MLSQKLKPTK
310 320 330 340 350
ERNPFKRCKR RLDYFIEDNW KRIWVMALWL SICAGLFTWK FIQYKRRAVF
360 370 380 390 400
DVMGYCVSVA KGGAETTKFN MALVLLPVCR NTITWLRSRT KLGKIIPFDD
410 420 430 440 450
NINFHKVIAF GIAVGVGLHA ISHLTCDFPR LLHATDEEYE PMKPFFGDER
460 470 480 490 500
PNNYWWFVKG TEGWTGVVMV VLMIIAYVLA QPWFRRNRLN LPSTIKKLTG
510 520 530 540 550
FNAFWYSHHL FVIVYVLFII HGYFLYLSKK WYKKTTWMYI AVPMILYACE
560 570 580 590 600
RLLRAFRSGY KAVKILKVAV YPGNVMAVHM SKPQGFKYTS GQYIFVNCSD
610 620 630 640 650
VSSFQWHPFT ISSAPGDDYL SMHIRTLGDW TSQLKTLFSK VCEPPTGDQS
660 670 680 690 700
GLLRADVAKA DYKPRLPKLL IDGPYGAPAQ DYKKYDVVLL VGLGIGATPL
710 720 730 740 750
ISIVKDVLNN IKQQKNIEDG TKGSKRSPFA TKRAYFYWVT REQGSFEWFK
760 770 780 790 800
GVMDEVSEND QEGLIELHNY CTSVYEEGDA RSALITMLQS IQQAKSGVDI
810 820 830 840 850
VSGTRVKTHF ARPNWRQVFK RVTINHPDQR IGVFYCGPQG LVGELRHLSQ
860
DFSHKTGTKF EFHKENF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB050661 mRNA Translation: BAB70751.1 |
RefSeqi | NP_001274981.1, NM_001288052.1 |
Genome annotation databases
GeneIDi | 102603596 |
KEGGi | sot:102603596 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB050661 mRNA Translation: BAB70751.1 |
RefSeqi | NP_001274981.1, NM_001288052.1 |
3D structure databases
SMRi | Q948T9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4113.PGSC0003DMT400063688 |
Protein family/group databases
PeroxiBasei | 4546, StRboh02 |
PTM databases
iPTMneti | Q948T9 |
Genome annotation databases
GeneIDi | 102603596 |
KEGGi | sot:102603596 |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
InParanoidi | Q948T9 |
OrthoDBi | 936110at2759 |
Gene expression databases
ExpressionAtlasi | Q948T9, baseline |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR013623, NADPH_Ox IPR029654, RBOHB IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR11972:SF64, PTHR11972:SF64, 1 hit |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit PF08414, NADPH_Ox, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SMARTi | View protein in SMART SM00054, EFh, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBOHB_SOLTU | |
Accessioni | Q948T9Primary (citable) accession number: Q948T9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | December 1, 2001 | |
Last modified: | June 2, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families