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UniProtKB - Q948T6 (LGUL_ORYSJ)
Protein
Lactoylglutathione lyase
Gene
GLYI-11
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione (By similarity).
Involved in the detoxifiation of methylglyoxal. Can functionally complement growth defect of a yeast mutant lacking GLY I. Involved in abiotic stress response. Over-expression of GLYI-11 in tobacco increases tolerance to osmotic, oxidative and salt stresses (PubMed:24661284).
By similarity1 PublicationMiscellaneous
Plants over-expressing GLYI-11 display increased tolerance to osmotic, oxidative and salt stresses.1 Publication
Catalytic activityi
- EC:4.4.1.51 Publication
Cofactori
Ni2+1 PublicationNote: Binds 1 nickel ion per subunit.1 Publication
Kineticsi
- KM=7.6 mM for methylglyoxal1 Publication
- KM=99.8 µM for glutatione1 Publication
- Vmax=130.8 µmol/min/mg enzyme with glutatione as substrate1 Publication
pH dependencei
Optimum pH is 7.2.1 Publication
: methylglyoxal degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (R)-lactate from methylglyoxal.Curated This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 31 | SubstrateBy similarity | 1 | |
Binding sitei | 82 | SubstrateBy similarity | 1 | |
Active sitei | 96 | 1 Publication | 1 | |
Binding sitei | 96 | SubstrateBy similarity | 1 | |
Active sitei | 145 | Proton donor/acceptor1 Publication | 1 | |
Metal bindingi | 145 | Nickel1 Publication | 1 | |
Active sitei | 158 | 1 Publication | 1 | |
Active sitei | 209 | 1 Publication | 1 | |
Metal bindingi | 209 | Nickel1 Publication | 1 |
GO - Molecular functioni
- IgE binding Source: UniProtKB
- lactoylglutathione lyase activity Source: UniProtKB
- nickel cation binding Source: UniProtKB
GO - Biological processi
- methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: UniProtKB
- response to osmotic stress Source: UniProtKB
- response to oxidative stress Source: UniProtKB
- response to salt stress Source: UniProtKB
Keywordsi
Molecular function | Lyase |
Ligand | Metal-binding, Nickel |
Enzyme and pathway databases
BRENDAi | 4.4.1.5, 8948 |
UniPathwayi | UPA00619;UER00675 |
Names & Taxonomyi
Protein namesi | Recommended name: Lactoylglutathione lyase (EC:4.4.1.51 Publication)Alternative name(s): Aldoketomutase Allergen Glb33 Glyoxalase I Short name: Glx I Glyoxylase I 11Curated Short name: OsGLYI-111 Publication Short name: OsGLYI111 Publication Ketone-aldehyde mutase Methylglyoxalase PP33 S-D-lactoylglutathione methylglyoxal lyase Allergen: Ory s Glyoxalase I |
Gene namesi | Name:GLYI-111 Publication Synonyms:GLX-I Ordered Locus Names:Os08g0191700, LOC_Os08g09250 ORF Names:OSJNBa0056O06.9-1 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Pathology & Biotechi
Allergenic propertiesi
Causes an allergic reaction in human. Binds to IgE.3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | E → Q: No effect on the activity. 1 Publication | 1 | |
Mutagenesisi | 145 | E → Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 209 | E → D: Loss of activity. 1 Publication | 1 |
Keywords - Diseasei
AllergenProtein family/group databases
Allergomei | 1048, Ory s Glyoxalase I |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168085 | 1 – 291 | Lactoylglutathione lyaseAdd BLAST | 291 |
Post-translational modificationi
Phosphorylated after gibberellin treatment.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q948T6 |
Expressioni
Tissue specificityi
Expressed in callus, stem, leaves, panicles and maturing seeds (at protein level).2 Publications
Inductioni
By salt stress and hydrogen peroxide (PubMed:21213008). Induced by methylglyoxal (PubMed:24661284).2 Publications
Gene expression databases
ExpressionAtlasi | Q948T6, baseline and differential |
Genevisiblei | Q948T6, OS |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 4530.OS08T0191700-01 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 149 | VOC 1PROSITE-ProRule annotationAdd BLAST | 126 | |
Domaini | 155 – 283 | VOC 2PROSITE-ProRule annotationAdd BLAST | 129 |
Sequence similaritiesi
Belongs to the glyoxalase I family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2943, Eukaryota |
HOGENOMi | CLU_030607_2_0_1 |
InParanoidi | Q948T6 |
OrthoDBi | 1513831at2759 |
Family and domain databases
Gene3Di | 3.10.180.10, 2 hits |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR004361, Glyoxalase_1 IPR018146, Glyoxalase_1_CS IPR037523, VOC |
Pfami | View protein in Pfam PF00903, Glyoxalase, 2 hits |
SUPFAMi | SSF54593, SSF54593, 2 hits |
TIGRFAMsi | TIGR00068, glyox_I, 2 hits |
PROSITEi | View protein in PROSITE PS00934, GLYOXALASE_I_1, 1 hit PS51819, VOC, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q948T6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASGSEAEKS PEVVLEWPKK DKKRLLHAVY RVGDLDRTIK CYTECFGMKL
60 70 80 90 100
LRKRDVPEEK YTNAFLGFGP EDTNFALELT YNYGVDKYDI GAGFGHFAIA
110 120 130 140 150
TEDVYKLAEK IKSSCCCKIT REPGPVKGGS TVIAFAQDPD GYMFELIQRG
160 170 180 190 200
PTPEPLCQVM LRVGDLDRSI KFYEKALGMK LLRKKDVPDY KYTIAMLGYA
210 220 230 240 250
DEDKTTVIEL TYNYGVTEYT KGNAYAQVAI GTEDVYKSAE AVELVTKELG
260 270 280 290
GKILRQPGPL PGLNTKIASF LDPDGWKVVL VDNADFLKEL Q
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0P0XCY4 | A0A0P0XCY4_ORYSJ | Glyoxalase I | Os08g0191700, OSNPB_080191700 | 292 | Annotation score: | ||
B7F6T2 | B7F6T2_ORYSJ | Os08g0191700 protein | Os08g0191700, OSNPB_080191700 | 181 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | L → V in BAB71741 (PubMed:11139585).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017042 mRNA Translation: BAA36759.1 EF122487 mRNA Translation: ABL74574.1 GQ848063 mRNA Translation: ADM86876.1 AB050986 Genomic DNA Translation: BAB71741.1 AP005441 Genomic DNA Translation: BAD05593.1 AP008214 Genomic DNA Translation: BAF23086.1 AP014964 Genomic DNA Translation: BAT04178.1 AK066092 mRNA Translation: BAG89815.1 AK103694 mRNA Translation: BAG96209.1 |
RefSeqi | XP_015650705.1, XM_015795219.1 |
Genome annotation databases
EnsemblPlantsi | Os08t0191700-01; Os08t0191700-01; Os08g0191700 Os08t0191700-03; Os08t0191700-03; Os08g0191700 |
GeneIDi | 4344858 |
Gramenei | Os08t0191700-01; Os08t0191700-01; Os08g0191700 Os08t0191700-03; Os08t0191700-03; Os08g0191700 |
KEGGi | osa:4344858 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017042 mRNA Translation: BAA36759.1 EF122487 mRNA Translation: ABL74574.1 GQ848063 mRNA Translation: ADM86876.1 AB050986 Genomic DNA Translation: BAB71741.1 AP005441 Genomic DNA Translation: BAD05593.1 AP008214 Genomic DNA Translation: BAF23086.1 AP014964 Genomic DNA Translation: BAT04178.1 AK066092 mRNA Translation: BAG89815.1 AK103694 mRNA Translation: BAG96209.1 |
RefSeqi | XP_015650705.1, XM_015795219.1 |
3D structure databases
SMRi | Q948T6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS08T0191700-01 |
Protein family/group databases
Allergomei | 1048, Ory s Glyoxalase I |
Proteomic databases
PaxDbi | Q948T6 |
Genome annotation databases
EnsemblPlantsi | Os08t0191700-01; Os08t0191700-01; Os08g0191700 Os08t0191700-03; Os08t0191700-03; Os08g0191700 |
GeneIDi | 4344858 |
Gramenei | Os08t0191700-01; Os08t0191700-01; Os08g0191700 Os08t0191700-03; Os08t0191700-03; Os08g0191700 |
KEGGi | osa:4344858 |
Phylogenomic databases
eggNOGi | KOG2943, Eukaryota |
HOGENOMi | CLU_030607_2_0_1 |
InParanoidi | Q948T6 |
OrthoDBi | 1513831at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00675 |
BRENDAi | 4.4.1.5, 8948 |
Gene expression databases
ExpressionAtlasi | Q948T6, baseline and differential |
Genevisiblei | Q948T6, OS |
Family and domain databases
Gene3Di | 3.10.180.10, 2 hits |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR004361, Glyoxalase_1 IPR018146, Glyoxalase_1_CS IPR037523, VOC |
Pfami | View protein in Pfam PF00903, Glyoxalase, 2 hits |
SUPFAMi | SSF54593, SSF54593, 2 hits |
TIGRFAMsi | TIGR00068, glyox_I, 2 hits |
PROSITEi | View protein in PROSITE PS00934, GLYOXALASE_I_1, 1 hit PS51819, VOC, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | LGUL_ORYSJ | |
Accessioni | Q948T6Primary (citable) accession number: Q948T6 Secondary accession number(s): Q0J7H9, Q9ZWJ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Allergens
Nomenclature of allergens and list of entries - Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families