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UniProtKB - Q947C4 (TPSD1_GINBI)
Protein
Bifunctional levopimaradiene synthase, chloroplastic
Gene
LPS
Organism
Ginkgo biloba (Ginkgo) (Maidenhair tree)
Status
Functioni
Catalyzes the initial cyclization step in the biosynthesis of ginkgolides, a structurally unique family of diterpenoids that are highly specific platelet-activating-factor receptor antagonists. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to levopimaradiene.
1 PublicationCatalytic activityi
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
pH dependencei
Optimum pH is 7.2.
: ginkgolide biosynthesis Pathwayi
This protein is involved in the pathway ginkgolide biosynthesis, which is part of Terpene metabolism.View all proteins of this organism that are known to be involved in the pathway ginkgolide biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 271 | SubstrateBy similarity | 1 | |
Metal bindingi | 405 | Magnesium 4By similarity | 1 | |
Metal bindingi | 407 | Magnesium 4By similarity | 1 | |
Binding sitei | 492 | SubstrateBy similarity | 1 | |
Metal bindingi | 624 | Magnesium 1By similarity | 1 | |
Metal bindingi | 624 | Magnesium 2By similarity | 1 | |
Metal bindingi | 628 | Magnesium 1By similarity | 1 | |
Metal bindingi | 628 | Magnesium 2By similarity | 1 | |
Metal bindingi | 769 | Magnesium 3By similarity | 1 | |
Metal bindingi | 773 | Magnesium 3By similarity | 1 | |
Metal bindingi | 777 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- copalyl diphosphate synthase activity Source: UniProtKB-EC
- levopimaradiene synthase activity Source: UniProtKB-EC
- magnesium ion binding Source: InterPro
- terpene synthase activity Source: InterPro
GO - Biological processi
- diterpenoid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 4.2.3.32, 2435 |
UniPathwayi | UPA00961 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:LPS |
Organismi | Ginkgo biloba (Ginkgo) (Maidenhair tree) |
Taxonomic identifieri | 3311 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Ginkgoidae › Ginkgoales › Ginkgoaceae › Ginkgo |
Subcellular locationi
Chloroplast
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 59 | ChloroplastSequence analysisAdd BLAST | 59 | |
ChainiPRO_0000348950 | 60 – 873 | Bifunctional levopimaradiene synthase, chloroplasticAdd BLAST | 814 |
Expressioni
Tissue specificityi
Expressed in roots.1 Publication
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 405 – 408 | DXDD motifCurated | 4 | |
Motifi | 624 – 628 | DDXXD motifCurated | 5 |
Domaini
The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity in the class II active site relevant for the cyclization of GGPP. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.Curated
Sequence similaritiesi
Keywords - Domaini
Transit peptideFamily and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q947C4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGVLFANLP CSLQLSPKVP FRQSTNILIP FHKRSSFGFN AQHCVRSHLR
60 70 80 90 100
LRWNCVGIHA SAAETRPDQL PQEERFVSRL NADYHPAVWK DDFIDSLTSP
110 120 130 140 150
NSHATSKSSV DETINKRIQT LVKEIQCMFQ SMGDGETNPS AYDTAWVARI
160 170 180 190 200
PSIDGSGAPQ FPQTLQWILN NQLPDGSWGE ECIFLAYDRV LNTLACLLTL
210 220 230 240 250
KIWNKGDIQV QKGVEFVRKH MEEMKDEADN HRPSGFEVVF PAMLDEAKSL
260 270 280 290 300
GLDLPYHLPF ISQIHQKRQK KLQKIPLNVL HNHQTALLYS LEGLQDVVDW
310 320 330 340 350
QEITNLQSRD GSFLSSPAST ACVFMHTQNK RCLHFLNFVL SKFGDYVPCH
360 370 380 390 400
YPLDLFERLW AVDTVERLGI DRYFKKEIKE SLDYVYRYWD AERGVGWARC
410 420 430 440 450
NPIPDVDDTA MGLRILRLHG YNVSSDVLEN FRDEKGDFFC FAGQTQIGVT
460 470 480 490 500
DNLNLYRCSQ VCFPGEKIME EAKTFTTNHL QNALAKNNAF DKWAVKKDLP
510 520 530 540 550
GEVEYAIKYP WHRSMPRLEA RSYIEQFGSN DVWLGKTVYK MLYVSNEKYL
560 570 580 590 600
ELAKLDFNMV QALHQKETQH IVSWWRESGF NDLTFTRQRP VEMYFSVAVS
610 620 630 640 650
MFEPEFAACR IAYAKTSCLA VILDDLYDTH GSLDDLKLFS EAVRRWDISV
660 670 680 690 700
LDSVRDNQLK VCFLGLYNTV NGFGKDGLKE QGRDVLGYLR KVWEGLLASY
710 720 730 740 750
TKEAEWSAAK YVPTFNEYVE NAKVSIALAT VVLNSIFFTG ELLPDYILQQ
760 770 780 790 800
VDLRSKFLHL VSLTGRLIND TKTYQAERNR GELVSSVQCY MRENPECTEE
810 820 830 840 850
EALSHVYGII DNALKELNWE LANPASNAPL CVRRLLFNTA RVMQLFYMYR
860 870
DGFGISDKEM KDHVSRTLFD PVA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF331704 mRNA Translation: AAL09965.1 AY574248 Genomic DNA Translation: AAS89668.1 |
Genome annotation databases
KEGGi | ag:AAL09965 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF331704 mRNA Translation: AAL09965.1 AY574248 Genomic DNA Translation: AAS89668.1 |
3D structure databases
SMRi | Q947C4 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AAL09965 |
Enzyme and pathway databases
UniPathwayi | UPA00961 |
BRENDAi | 4.2.3.32, 2435 |
Family and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPSD1_GINBI | |
Accessioni | Q947C4Primary (citable) accession number: Q947C4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | December 1, 2001 | |
Last modified: | September 29, 2021 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families