UniProtKB - Q94535 (U2AF1_DROME)
Protein
Splicing factor U2af 38 kDa subunit
Gene
U2af38
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Necessary for the splicing of pre-mRNA. Binds to the polypyrimidine tract of introns early during spliceosome assembly (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 12 – 40 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 151 – 178 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 28 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- mRNA binding Source: FlyBase
- poly-pyrimidine tract binding Source: FlyBase
- pre-mRNA 3'-splice site binding Source: GO_Central
GO - Biological processi
- mRNA splicing, via spliceosome Source: FlyBase
- regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
- RNA splicing Source: FlyBase
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-DME-72163, mRNA Splicing - Major Pathway R-DME-72187, mRNA 3'-end processing R-DME-73856, RNA Polymerase II Transcription Termination |
Names & Taxonomyi
Protein namesi | Recommended name: Splicing factor U2af 38 kDa subunitAlternative name(s): U2 auxiliary factor 38 kDa subunit U2 snRNP auxiliary factor small subunit |
Gene namesi | Name:U2af38 ORF Names:CG3582 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0017457, U2af38 |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: FlyBase
- precatalytic spliceosome Source: FlyBase
- spliceosomal complex Source: GO_Central
- U2AF complex Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081997 | 1 – 264 | Splicing factor U2af 38 kDa subunitAdd BLAST | 264 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 19 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q94535 |
PRIDEi | Q94535 |
PTM databases
iPTMneti | Q94535 |
Expressioni
Gene expression databases
Bgeei | FBgn0017457, Expressed in embryo and 38 other tissues |
ExpressionAtlasi | Q94535, baseline and differential |
Genevisiblei | Q94535, DM |
Interactioni
Subunit structurei
Associates with a 65 kDa protein.
By similarityBinary interactionsi
Q94535
With | #Exp. | IntAct |
---|---|---|
U2af50 [Q24562] | 4 | EBI-121011,EBI-165226 |
Protein-protein interaction databases
BioGRIDi | 59457, 5 interactors |
DIPi | DIP-23299N |
IntActi | Q94535, 4 interactors |
STRINGi | 7227.FBpp0305600 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 149 | RRMPROSITE-ProRule annotationAdd BLAST | 106 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 180 – 213 | Arg/Ser-rich (RS domain)Add BLAST | 34 | |
Compositional biasi | 190 – 197 | Poly-Arg | 8 | |
Compositional biasi | 252 – 262 | Poly-GlyAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the splicing factor SR family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 12 – 40 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 151 – 178 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 28 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2202, Eukaryota |
GeneTreei | ENSGT00950000183152 |
HOGENOMi | CLU_059852_1_0_1 |
InParanoidi | Q94535 |
OMAi | FMHLRLA |
OrthoDBi | 1340384at2759 |
PhylomeDBi | Q94535 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk IPR009145, U2AF_small IPR000571, Znf_CCCH |
PANTHERi | PTHR12620, PTHR12620, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF00642, zf-CCCH, 2 hits |
PRINTSi | PR01848, U2AUXFACTOR |
SMARTi | View protein in SMART SM00361, RRM_1, 1 hit SM00356, ZnF_C3H1, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit PS50103, ZF_C3H1, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q94535-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEYLASIFG TEKDKVNCSF YFKIGACRHG DRCSRIHNKP TFSQTVLLQN
60 70 80 90 100
LYVNPQNSAK SADGSHLVAN VSDEEMQEHY DNFFEDVFVE CEDKYGEIEE
110 120 130 140 150
MNVCDNLGDH LVGNVYIKFR NEADAEKAAN DLNNRWFGGR PVYSELSPVT
160 170 180 190 200
DFREACCRQY EMGECTRSGF CNFMHLKPIS RELRRYLYSR RRRARSRSRS
210 220 230 240 250
PGRRRGSRSR SRSPGRRGGG RGDGVGGGNY LNNERDNMRG NDRGNDRDRR
260
KGGGGGGGGG GGRY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PBM1 | M9PBM1_DROME | U2 small nuclear riboprotein auxili... | U2af38 Dmel\CG3582, DU2AF38, dU2AF38, dU2AF[38], l(2)06751 | 264 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 66 | H → D in AAB17271 (PubMed:8816800).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67066 mRNA Translation: AAB17271.1 AE014134 Genomic DNA Translation: AAF51512.1 AY058537 mRNA Translation: AAL13766.1 |
PIRi | JC6125 |
RefSeqi | NP_001259814.1, NM_001272885.1 NP_477208.1, NM_057860.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0078133; FBpp0077792; FBgn0017457 FBtr0333408; FBpp0305600; FBgn0017457 |
GeneIDi | 33201 |
KEGGi | dme:Dmel_CG3582 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67066 mRNA Translation: AAB17271.1 AE014134 Genomic DNA Translation: AAF51512.1 AY058537 mRNA Translation: AAL13766.1 |
PIRi | JC6125 |
RefSeqi | NP_001259814.1, NM_001272885.1 NP_477208.1, NM_057860.4 |
3D structure databases
SMRi | Q94535 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 59457, 5 interactors |
DIPi | DIP-23299N |
IntActi | Q94535, 4 interactors |
STRINGi | 7227.FBpp0305600 |
PTM databases
iPTMneti | Q94535 |
Proteomic databases
PaxDbi | Q94535 |
PRIDEi | Q94535 |
Genome annotation databases
EnsemblMetazoai | FBtr0078133; FBpp0077792; FBgn0017457 FBtr0333408; FBpp0305600; FBgn0017457 |
GeneIDi | 33201 |
KEGGi | dme:Dmel_CG3582 |
Organism-specific databases
CTDi | 33201 |
FlyBasei | FBgn0017457, U2af38 |
Phylogenomic databases
eggNOGi | KOG2202, Eukaryota |
GeneTreei | ENSGT00950000183152 |
HOGENOMi | CLU_059852_1_0_1 |
InParanoidi | Q94535 |
OMAi | FMHLRLA |
OrthoDBi | 1340384at2759 |
PhylomeDBi | Q94535 |
Enzyme and pathway databases
Reactomei | R-DME-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-DME-72163, mRNA Splicing - Major Pathway R-DME-72187, mRNA 3'-end processing R-DME-73856, RNA Polymerase II Transcription Termination |
Miscellaneous databases
BioGRID-ORCSi | 33201, 0 hits in 3 CRISPR screens |
ChiTaRSi | U2af38, fly |
GenomeRNAii | 33201 |
PROi | PR:Q94535 |
Gene expression databases
Bgeei | FBgn0017457, Expressed in embryo and 38 other tissues |
ExpressionAtlasi | Q94535, baseline and differential |
Genevisiblei | Q94535, DM |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk IPR009145, U2AF_small IPR000571, Znf_CCCH |
PANTHERi | PTHR12620, PTHR12620, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF00642, zf-CCCH, 2 hits |
PRINTSi | PR01848, U2AUXFACTOR |
SMARTi | View protein in SMART SM00361, RRM_1, 1 hit SM00356, ZnF_C3H1, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit PS50103, ZF_C3H1, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | U2AF1_DROME | |
Accessioni | Q94535Primary (citable) accession number: Q94535 Secondary accession number(s): Q9VPN4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families