Dynein light chain Tctex-type

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• disordered domain specific binding Source: CAFA <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "Interactions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74."
    Makokha M., Hare M., Li M., Hays T., Barbar E.
    Biochemistry 41:4302-4311(2002) [PubMed] [Europe PMC] [Abstract]
  • "Characterization of the cargo attachment complex of cytoplasmic dynein using NMR and mass spectrometry."
    Barbar E., Hare M.
    Methods Enzymol 380:219-241(2004) [PubMed] [Europe PMC] [Abstract]
  • "Multivalency in the assembly of intrinsically disordered Dynein intermediate chain."
    Hall J., Karplus P.A., Barbar E.
    J Biol Chem 284:33115-33121(2009) [PubMed] [Europe PMC] [Abstract]
• dynein intermediate chain binding Source: FlyBaseInferred from physical interactionⁱ
  • "Potential role for phosphorylation in differential regulation of the assembly of dynein light chains."
    Song Y., Benison G., Nyarko A., Hays T.S., Barbar E.
    J Biol Chem 282:17272-17279(2007) [PubMed] [Europe PMC] [Abstract]
• dynein light intermediate chain binding Source: FlyBase <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex."
    Williams J.C., Roulhac P.L., Roy A.G., Vallee R.B., Fitzgerald M.C., Hendrickson W.A.
    Proc Natl Acad Sci U S A 104:10028-10033(2007) [PubMed] [Europe PMC] [Abstract]
• identical protein binding Source: IntActInferred from physical interactionⁱ
  • "Crystal structure of dynein light chain TcTex-1."
    Williams J.C., Xie H., Hendrickson W.A.
    J Biol Chem 280:21981-21986(2005) [PubMed] [Europe PMC] [Abstract]
• protein homodimerization activity Source: FlyBaseInferred from direct assayⁱ
  • "Potential role for phosphorylation in differential regulation of the assembly of dynein light chains."
    Song Y., Benison G., Nyarko A., Hays T.S., Barbar E.
    J Biol Chem 282:17272-17279(2007) [PubMed] [Europe PMC] [Abstract]
  • "Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex."
    Williams J.C., Roulhac P.L., Roy A.G., Vallee R.B., Fitzgerald M.C., Hendrickson W.A.
    Proc Natl Acad Sci U S A 104:10028-10033(2007) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• determination of adult lifespan Source: FlyBase <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Misexpression screen delineates novel genes controlling Drosophila lifespan."
    Paik D., Jang Y.G., Lee Y.E., Lee Y.N., Yamamoto R., Gee H.Y., Yoo S., Bae E., Min K.J., Tatar M., Park J.J.
    Mech Ageing Dev 133:234-245(2012) [PubMed] [Europe PMC] [Abstract]
• microtubule-based movement Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• mitotic cell cycle Source: FlyBaseInferred from high throughput mutant phenotypeⁱ
  • "Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin."
    Ducat D., Kawaguchi S., Liu H., Yates J.R., Zheng Y.
    Mol Biol Cell 19:3097-3110(2008) [PubMed] [Europe PMC] [Abstract]
• retrograde axonal transport Source: FlyBaseInferred from mutant phenotypeⁱ
  • "Dynein-dynactin complex is essential for dendritic restriction of TM1-containing Drosophila Dscam."
    Yang J.S., Bai J.M., Lee T.
    PLoS One 3:e3504-e3504(2008) [PubMed] [Europe PMC] [Abstract]
• spermatid development Source: FlyBaseInferred from mutant phenotypeⁱ
  • Ref.6

    "The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation."
    Li M.G., Serr M., Newman E.A., Hays T.S.
    Mol. Biol. Cell 15:3005-3014(2004) [PubMed] [Europe PMC] [Abstract]

    Cited for: SUBUNIT, FUNCTION, DISRUPTION PHENOTYPE.

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

• UniProt annotation
• GO - Cellular component

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypeⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• disordered domain specific binding Source: CAFAInferred from physical interactionⁱ
  • "Interactions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74."
    Makokha M., Hare M., Li M., Hays T., Barbar E.
    Biochemistry 41:4302-4311(2002) [PubMed] [Europe PMC] [Abstract]
  • "Characterization of the cargo attachment complex of cytoplasmic dynein using NMR and mass spectrometry."
    Barbar E., Hare M.
    Methods Enzymol 380:219-241(2004) [PubMed] [Europe PMC] [Abstract]
  • "Multivalency in the assembly of intrinsically disordered Dynein intermediate chain."
    Hall J., Karplus P.A., Barbar E.
    J Biol Chem 284:33115-33121(2009) [PubMed] [Europe PMC] [Abstract]
• dynein intermediate chain binding Source: FlyBaseInferred from physical interactionⁱ
  • "Potential role for phosphorylation in differential regulation of the assembly of dynein light chains."
    Song Y., Benison G., Nyarko A., Hays T.S., Barbar E.
    J Biol Chem 282:17272-17279(2007) [PubMed] [Europe PMC] [Abstract]
• dynein light intermediate chain binding Source: FlyBaseInferred from direct assayⁱ
  • "Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex."
    Williams J.C., Roulhac P.L., Roy A.G., Vallee R.B., Fitzgerald M.C., Hendrickson W.A.
    Proc Natl Acad Sci U S A 104:10028-10033(2007) [PubMed] [Europe PMC] [Abstract]
• identical protein binding Source: IntActInferred from physical interactionⁱ
  • "Crystal structure of dynein light chain TcTex-1."
    Williams J.C., Xie H., Hendrickson W.A.
    J Biol Chem 280:21981-21986(2005) [PubMed] [Europe PMC] [Abstract]
• protein homodimerization activity Source: FlyBaseInferred from direct assayⁱ
  • "Potential role for phosphorylation in differential regulation of the assembly of dynein light chains."
    Song Y., Benison G., Nyarko A., Hays T.S., Barbar E.
    J Biol Chem 282:17272-17279(2007) [PubMed] [Europe PMC] [Abstract]
  • "Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex."
    Williams J.C., Roulhac P.L., Roy A.G., Vallee R.B., Fitzgerald M.C., Hendrickson W.A.
    Proc Natl Acad Sci U S A 104:10028-10033(2007) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MDDSREESQF IVDDVSKTIK EAIETTIGGN AYQHDKVNNW TGQVVENCLT 
        60         70         80         90        100
VLTKEQKPYK YIVTAMIMQK NGAGLHTASS CYWNNDTDGS CTVRWENKTM 
       110 
YCIVSVFGLA V                                           

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Drosophila
   Drosophila: entries, gene names and cross-references to FlyBase
2. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
3. SIMILARITY comments
   Index of protein domains and families