Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

ND-75

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi85Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi88Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi102Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi134Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi138Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi141Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi147Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi190Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi193Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi196Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi240Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-DME-611105 Respiratory electron transport
R-DME-6799198 Complex I biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
Gene namesi
Name:ND-75Imported
Synonyms:ND75
ORF Names:CG2286Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0017566 ND-75

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000001997128 – 731NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialAdd BLAST704

Proteomic databases

PaxDbiQ94511
PRIDEiQ94511

Expressioni

Gene expression databases

BgeeiFBgn0017566
ExpressionAtlasiQ94511 baseline and differential
GenevisibleiQ94511 DM

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits.By similarity

Protein-protein interaction databases

BioGridi58223, 30 interactors
DIPiDIP-17348N
IntActiQ94511, 8 interactors
MINTiQ94511
STRINGi7227.FBpp0071128

Structurei

3D structure databases

ProteinModelPortaliQ94511
SMRiQ94511
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 1182Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini118 – 1574Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini259 – 3154Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2282 Eukaryota
COG1034 LUCA
GeneTreeiENSGT00390000018768
HOGENOMiHOG000264012
InParanoidiQ94511
KOiK03934
OMAiPQASCAM
OrthoDBiEOG091G02C2
PhylomeDBiQ94511

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NuoG_C
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
PF09326 NADH_dhqG_C, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRAPLVKAL GALGSPTHQM ASRAVRTSAM VAQTPAKAPE KIEVFVDDIP
60 70 80 90 100
VQVVPGTTVL QAAAQIGVEI PRFCYHERLA VAGNCRMCLV EVEKSPKPVA
110 120 130 140 150
ACAMPVMKGW RIKTNSDLTR KAREGVMEFL LMNHPLDCPI CDQGGECDLQ
160 170 180 190 200
DQAMAFGSDR SRFTDINYTG KRAVEDKDIG PLVKTIMTRC IHCTRCVRFA
210 220 230 240 250
SEIAGVDDLG TTGRGNDMQI GTYVEKLFLT ELSGNVIDLC PVGALTNKPY
260 270 280 290 300
SFVARPWEIR KVSSIDVLDA VGSNIVVSTR TNEVLRILPR ENEDVNEEWL
310 320 330 340 350
ADKSRFACDG LKRQRLVAPM VRMPNGELQA VEWEGALIAV AKAIKAAGGQ
360 370 380 390 400
IAGISGQLAD LEAQVALKDL LNRLGSEVVA TEQGFIAGGT DNRANYLLNS
410 420 430 440 450
TIAGLEEADA VLLVGTNPRY EAPLVNTRLR KAYVHNELQI ASIGPKIDLS
460 470 480 490 500
YDHENLGADA ALVKDVCSGA HAFSKVLEGA KKPAIIIGAD LLERADGAAI
510 520 530 540 550
HATVAEYCKK LKKPNWNPFN VLQTNAAQVG ALDVGYKAGA QTAVKAQPKV
560 570 580 590 600
LFLLNADAGK VTREQLPKDC FVVYIGSHGD NGASIADAVL PGAAYTEKQG
610 620 630 640 650
IYVNTEGRPQ QTLPGVSPPG MAREDWKILR ALSEVVGKPL PYDNLDELRN
660 670 680 690 700
RLEDVAPHLT RLGQLEPAGD AGAAGTISKS IGGGAIDIKL KELRDYFMTD
710 720 730
AISRASPTMA KCISAVNKQQ RENEAKQSVA I
Length:731
Mass (Da):78,630
Last modified:February 21, 2001 - v3
Checksum:iB3CA26B56AFACBFD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65Q → L in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti94K → N in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti111R → P in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti120R → P in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti470A → S in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti611Q → H in CAA70284 (PubMed:10071211).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA Translation: AAF46356.1
Y09063 mRNA Translation: CAA70284.1
RefSeqiNP_511083.1, NM_078528.3
NP_727255.1, NM_167152.2
UniGeneiDm.6840

Genome annotation databases

EnsemblMetazoaiFBtr0071180; FBpp0071128; FBgn0017566
FBtr0071181; FBpp0071129; FBgn0017566
GeneIDi31762
KEGGidme:Dmel_CG2286

Similar proteinsi

Entry informationi

Entry nameiNDUS1_DROME
AccessioniPrimary (citable) accession number: Q94511
Secondary accession number(s): Q9W3H1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 21, 2001
Last modified: July 18, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health