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UniProtKB - Q944G9 (ALFP2_ARATH)
Protein
Fructose-bisphosphate aldolase 2, chloroplastic
Gene
FBA2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Plays a key role in glycolysis and gluconeogenesis.
By similarityCatalytic activityi
- β-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphateBy similarityEC:4.1.2.13By similarity
Kineticsi
- KM=35 µM for fructose-bisphosphate for the cleavage reaction1 Publication
- Vmax=13 µmol/min/mg enzyme with fructose-bisphosphate as substrate1 Publication
: glycolysis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 95 | SubstrateBy similarity | 1 | |
Binding sitei | 185 | SubstrateBy similarity | 1 | |
Active sitei | 225 | Proton acceptorBy similarity | 1 | |
Active sitei | 267 | Schiff-base intermediate with dihydroxyacetone-PBy similarity | 1 | |
Sitei | 398 | Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity | 1 |
GO - Molecular functioni
- fructose-bisphosphate aldolase activity Source: UniProtKB
- mRNA binding Source: TAIR
GO - Biological processi
- fructose 1,6-bisphosphate metabolic process Source: GO_Central
- gluconeogenesis Source: UniProtKB
- glycolytic process Source: UniProtKB
- response to abscisic acid Source: TAIR
Keywordsi
Molecular function | Lyase |
Biological process | Glycolysis |
Ligand | Schiff base |
Enzyme and pathway databases
BioCyci | ARA:AT4G38970-MONOMER |
UniPathwayi | UPA00109;UER00183 |
Names & Taxonomyi
Protein namesi | Recommended name: Fructose-bisphosphate aldolase 2, chloroplasticCurated (EC:4.1.2.13Curated)Short name: AtFBA21 Publication |
Gene namesi | Name:FBA21 Publication Ordered Locus Names:At4g38970 ORF Names:F19H22.70 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G38970 |
TAIRi | locus:2120192, AT4G38970 |
Subcellular locationi
Chloroplast
- plastoglobule 2 Publications
- chloroplast stroma 1 Publication
Chloroplast
- chloroplast Source: TAIR
- chloroplast envelope Source: TAIR
- chloroplast stroma Source: TAIR
- plastoglobule Source: TAIR
Cytosol
- cytosol Source: GO_Central
Extracellular region or secreted
- apoplast Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- thylakoid Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Reduced growth rate. Unability to accumulate starch in leaves during daylight.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 394 | K → A: Loss of methylation, but no effect on enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 46 | ChloroplastSequence analysisAdd BLAST | 46 | |
ChainiPRO_0000286527 | 47 – 398 | Fructose-bisphosphate aldolase 2, chloroplasticAdd BLAST | 352 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 157 | PhosphoserineCombined sources | 1 | |
Modified residuei | 215 | PhosphoserineCombined sources | 1 | |
Modified residuei | 394 | N6,N6,N6-trimethyllysine1 Publication | 1 |
Post-translational modificationi
Can be trimethylated at Lys-394 by LSMT-L. The methylation level has no influence on the ologomerization state or on the kinetic properties of the enzyme.1 Publication
Phosphorylated on tyrosine residues in response to abscisic acid (ABA) in germinating seeds.1 Publication
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
PaxDbi | Q944G9 |
PRIDEi | Q944G9 |
ProteomicsDBi | 244954 [Q944G9-1] |
PTM databases
iPTMneti | Q944G9 |
Expressioni
Tissue specificityi
Highly expressed in rosettes leaves.1 Publication
Inductioni
Gene expression databases
ExpressionAtlasi | Q944G9, baseline and differential |
Genevisiblei | Q944G9, AT |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 15332, 5 interactors |
STRINGi | 3702.AT4G38970.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 309 – 311 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the class I fructose-bisphosphate aldolase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1557, Eukaryota |
HOGENOMi | CLU_031243_0_0_1 |
InParanoidi | Q944G9 |
OMAi | MENTEAN |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR029768, Aldolase_I_AS IPR013785, Aldolase_TIM IPR000741, FBA_I |
PANTHERi | PTHR11627, PTHR11627, 1 hit |
Pfami | View protein in Pfam PF00274, Glycolytic, 1 hit |
PROSITEi | View protein in PROSITE PS00158, ALDOLASE_CLASS_I, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q944G9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASTSLLKAS PVLDKSEWVK GQSVLFRQPS SASVVLRNRA TSLTVRAASS
60 70 80 90 100
YADELVKTAK TIASPGRGIL AMDESNATCG KRLDSIGLEN TEANRQAFRT
110 120 130 140 150
LLVSAPGLGQ YVSGAILFEE TLYQSTTEGK KMVDVLVEQN IVPGIKVDKG
160 170 180 190 200
LVPLVGSNNE SWCQGLDGLS SRTAAYYQQG ARFAKWRTVV SIPNGPSALA
210 220 230 240 250
VKEAAWGLAR YAAISQDSGL VPIVEPEILL DGEHDIDRTY DVAEKVWAEV
260 270 280 290 300
FFYLAQNNVM FEGILLKPSM VTPGAESKDR ATPEQVAAYT LKLLRNRVPP
310 320 330 340 350
AVPGIMFLSG GQSEVEATLN LNAMNQAPNP WHVSFSYARA LQNTCLKTWG
360 370 380 390
GRPENVNAAQ TTLLARAKAN SLAQLGKYTG EGESEEAKEG MFVKGYTY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4JUJ5 | F4JUJ5_ARATH | Fructose-bisphosphate aldolase | FBA2 AtFBA2, At4g38970, F19H22.70, F19H22_70 | 381 | Annotation score: |
Sequence cautioni
The sequence CAB38817 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80560 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 163 | C → G in AAL16224 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL035679 Genomic DNA Translation: CAB38817.1 Sequence problems. AL161594 Genomic DNA Translation: CAB80560.1 Sequence problems. CP002687 Genomic DNA Translation: AEE87002.1 AF428455 mRNA Translation: AAL16224.1 BT015870 mRNA Translation: AAU94433.1 AK226247 mRNA Translation: BAE98409.1 |
PIRi | T06057 |
RefSeqi | NP_568049.1, NM_120057.4 [Q944G9-1] |
Genome annotation databases
EnsemblPlantsi | AT4G38970.1; AT4G38970.1; AT4G38970 [Q944G9-1] |
GeneIDi | 830052 |
Gramenei | AT4G38970.1; AT4G38970.1; AT4G38970 [Q944G9-1] |
KEGGi | ath:AT4G38970 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL035679 Genomic DNA Translation: CAB38817.1 Sequence problems. AL161594 Genomic DNA Translation: CAB80560.1 Sequence problems. CP002687 Genomic DNA Translation: AEE87002.1 AF428455 mRNA Translation: AAL16224.1 BT015870 mRNA Translation: AAU94433.1 AK226247 mRNA Translation: BAE98409.1 |
PIRi | T06057 |
RefSeqi | NP_568049.1, NM_120057.4 [Q944G9-1] |
3D structure databases
AlphaFoldDBi | Q944G9 |
SMRi | Q944G9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 15332, 5 interactors |
STRINGi | 3702.AT4G38970.1 |
PTM databases
iPTMneti | Q944G9 |
Proteomic databases
PaxDbi | Q944G9 |
PRIDEi | Q944G9 |
ProteomicsDBi | 244954 [Q944G9-1] |
Genome annotation databases
EnsemblPlantsi | AT4G38970.1; AT4G38970.1; AT4G38970 [Q944G9-1] |
GeneIDi | 830052 |
Gramenei | AT4G38970.1; AT4G38970.1; AT4G38970 [Q944G9-1] |
KEGGi | ath:AT4G38970 |
Organism-specific databases
Araporti | AT4G38970 |
TAIRi | locus:2120192, AT4G38970 |
Phylogenomic databases
eggNOGi | KOG1557, Eukaryota |
HOGENOMi | CLU_031243_0_0_1 |
InParanoidi | Q944G9 |
OMAi | MENTEAN |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00183 |
BioCyci | ARA:AT4G38970-MONOMER |
Miscellaneous databases
PROi | PR:Q944G9 |
Gene expression databases
ExpressionAtlasi | Q944G9, baseline and differential |
Genevisiblei | Q944G9, AT |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR029768, Aldolase_I_AS IPR013785, Aldolase_TIM IPR000741, FBA_I |
PANTHERi | PTHR11627, PTHR11627, 1 hit |
Pfami | View protein in Pfam PF00274, Glycolytic, 1 hit |
PROSITEi | View protein in PROSITE PS00158, ALDOLASE_CLASS_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALFP2_ARATH | |
Accessioni | Q944G9Primary (citable) accession number: Q944G9 Secondary accession number(s): Q5XEU6, Q9SVJ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 129 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families