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Protein

Protein maternal effect lethal 26

Gene

mel-26

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14528312, PubMed:13679922, PubMed:23918937). Controls degradation of microtubule severing protein mei-1 after meiosis (PubMed:14528312). Controls degradation of ppfr-1, the regulatory subunit of PP4 complex, after meiosis (PubMed:23918937). In body wall muscles, involved in the organization of myosin thick filaments, likely by regulating the degradation of mei-1 downstream of unc-89 (PubMed:22621901). May also activate the TORC1 pathway (PubMed:29769719).5 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein binding, bridging Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

SignaLinkiQ94420
UniPathwayi
UPA00143

Protein family/group databases

MoonDBiQ94420 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Protein maternal effect lethal 26
Gene namesi
Name:mel-26
ORF Names:ZK858.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiZK858.4a ; CE30580 ; WBGene00003209 ; mel-26

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown at L1 larval stage results in the disorganization of myosin thick filaments in adult body wall muscles characterized by the formation of abnormal myosin heavy chain myo-3 aggregates and V-shaped crossing of A-bands (PubMed:22621901). RNAi-mediated knockdown of mel-26 also results in increased lifespan (PubMed:29769719).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94C → Y: Severe loss of interaction with ppfr-1 and mei-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003126281 – 395Protein maternal effect lethal 26Add BLAST395

Proteomic databases

EPDiQ94420
PaxDbiQ94420

Expressioni

Tissue specificityi

Expressed in body wall muscles.1 Publication

Gene expression databases

BgeeiWBGene00003209 Expressed in 5 organ(s), highest expression level in adult organism

Interactioni

Subunit structurei

Interacts (via BTB domain) with cul-3 (PubMed:13679922, PubMed:14528312, PubMed:22621901). Seems to be a component of a E3 ubiquitin-protein ligase complex containing cul-3 (PubMed:13679922, PubMed:14528312). Interacts (probably via MATH domain) with mei-1, which targets mei-1 for ubiquitin-mediated proteolysis (PubMed:14528312, PubMed:22621901, PubMed:23918937). Interacts (probably via MATH domain) with ppfr-1, the regulatory subunit of the PP4 complex; targets ppfr-1 for ubiquitin-mediated proteolysis (PubMed:23918937). May interact (via MATH domain) with unc-89 (via Ig-like C2-type domain 2/3 and, Ig-like C2-type domain 50 and fibronectin type-III domain 2) (PubMed:22621901).4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi38168, 31 interactors
DIPiDIP-27179N
IntActiQ94420, 41 interactors
MINTiQ94420
STRINGi6239.ZK858.4

Structurei

3D structure databases

ProteinModelPortaliQ94420
SMRiQ94420
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 162MATHPROSITE-ProRule annotationAdd BLAST122
Domaini201 – 269BTBPROSITE-ProRule annotationAdd BLAST69

Phylogenomic databases

eggNOGiKOG1987 Eukaryota
ENOG410XQV8 LUCA
GeneTreeiENSGT00390000000361
HOGENOMiHOG000019663
InParanoidiQ94420
PhylomeDBiQ94420

Family and domain databases

CDDicd14734 SPOP_C, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR002083 MATH/TRAF_dom
IPR011333 SKP1/BTB/POZ_sf
IPR034089 SPOP_C
IPR008974 TRAF-like
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS50144 MATH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q94420-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPRIDGGVF IGGIGNSGNE MCSNGVPALG VSSQTEIKVE KVQHTWTVKN
60 70 80 90 100
FSHCYQEYLE NFVYLQRGDE QLTWSIKIYP KGNGENNKDF VFLCLNRVIN
110 120 130 140 150
NNVKAGKIGF KSQFKLRTAE NKDIEMRIHP NPSHSDYVSY IKRDVLFPQI
160 170 180 190 200
MPRDMIIVNV EIDVAVETIT TTNEPIQFEP TNSEQQLIED YQRLFSQELL
210 220 230 240 250
CDFAINVNGK IIRAHKAVLA ARSPVFNAML THQDTDEAKS SMMYINDMDY
260 270 280 290 300
DVIYEMVYYI YCGRCNKDIT DMATALLIAA DKYRLEELKS HCEKYLVENI
310 320 330 340 350
NIENACSLLI IGDLYSAPKL RKRAVTYILA RPKNVTGTPG WEDILKGHPN
360 370 380 390
LITDIFSQID RQSSTGATSS VSNLPGVPMD IPGITGNIVP PPSGL
Length:395
Mass (Da):44,492
Last modified:June 1, 2002 - v2
Checksum:i3AED944583A155AC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K8F7T2K8F7T2_CAEEL
Protein maternal effect lethal 26
mel-26 CELE_ZK858.4, ZK858.4
399Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67737 Genomic DNA Translation: AAC63596.1
Z79759 Genomic DNA Translation: CAB02139.2
PIRiT28056
RefSeqiNP_492449.2, NM_060048.8
UniGeneiCel.5086

Genome annotation databases

EnsemblMetazoaiZK858.4a; ZK858.4a; WBGene00003209
GeneIDi172737
UCSCiZK858.4 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67737 Genomic DNA Translation: AAC63596.1
Z79759 Genomic DNA Translation: CAB02139.2
PIRiT28056
RefSeqiNP_492449.2, NM_060048.8
UniGeneiCel.5086

3D structure databases

ProteinModelPortaliQ94420
SMRiQ94420
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38168, 31 interactors
DIPiDIP-27179N
IntActiQ94420, 41 interactors
MINTiQ94420
STRINGi6239.ZK858.4

Protein family/group databases

MoonDBiQ94420 Predicted

Proteomic databases

EPDiQ94420
PaxDbiQ94420

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK858.4a; ZK858.4a; WBGene00003209
GeneIDi172737
UCSCiZK858.4 c. elegans

Organism-specific databases

CTDi172737
WormBaseiZK858.4a ; CE30580 ; WBGene00003209 ; mel-26

Phylogenomic databases

eggNOGiKOG1987 Eukaryota
ENOG410XQV8 LUCA
GeneTreeiENSGT00390000000361
HOGENOMiHOG000019663
InParanoidiQ94420
PhylomeDBiQ94420

Enzyme and pathway databases

UniPathwayi
UPA00143

SignaLinkiQ94420

Miscellaneous databases

PROiPR:Q94420

Gene expression databases

BgeeiWBGene00003209 Expressed in 5 organ(s), highest expression level in adult organism

Family and domain databases

CDDicd14734 SPOP_C, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR002083 MATH/TRAF_dom
IPR011333 SKP1/BTB/POZ_sf
IPR034089 SPOP_C
IPR008974 TRAF-like
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS50144 MATH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMEL26_CAEEL
AccessioniPrimary (citable) accession number: Q94420
Secondary accession number(s): Q94139
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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