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UniProtKB - Q93Z25 (PLY22_ARATH)
Protein
Probable pectate lyase 22
Gene
At5g63180
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalytic activityi
- Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends. EC:4.2.2.2
Cofactori
Ca2+By similarityNote: Binds 1 Ca2+ ion. Required for its activity.By similarity
: pectin degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin. This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 228 | CalciumBy similarity | 1 | |
Metal bindingi | 252 | CalciumBy similarity | 1 | |
Metal bindingi | 256 | CalciumBy similarity | 1 | |
Active sitei | 308 | Sequence analysis | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- pectate lyase activity Source: UniProtKB-EC
GO - Biological processi
- pectin catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT5G63180-MONOMER |
UniPathwayi | UPA00545;UER00824 |
Protein family/group databases
CAZyi | PL1, Polysaccharide Lyase Family 1 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:At5g63180 ORF Names:MDC12_15 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G63180 |
TAIRi | locus:2161992, AT5G63180 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 45 | Sequence analysisAdd BLAST | 45 | |
ChainiPRO_0000024887 | 46 – 432 | Probable pectate lyase 22Add BLAST | 387 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 65 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q93Z25 |
PRIDEi | Q93Z25 |
ProteomicsDBi | 234676 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q93Z25, baseline and differential |
Genevisiblei | Q93Z25, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the polysaccharide lyase 1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QQ5F, Eukaryota |
HOGENOMi | CLU_026608_0_1_1 |
InParanoidi | Q93Z25 |
OMAi | CKRGSHC |
OrthoDBi | 924221at2759 |
PhylomeDBi | Q93Z25 |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR018082, AmbAllergen IPR002022, Pec_lyase IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR045032, PEL |
PANTHERi | PTHR31683, PTHR31683, 1 hit |
Pfami | View protein in Pfam PF00544, Pectate_lyase_4, 1 hit |
PRINTSi | PR00807, AMBALLERGEN |
SMARTi | View protein in SMART SM00656, Amb_all, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q93Z25-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRPNSLLIP SNLSTTKSQR NTMLNSSYLS FALIFFCCIL FSALASSLPV
60 70 80 90 100
SDPELVVEEV HRKINESISR RKLGFFSCGS GNPIDDCWRC DKDWEKNRKR
110 120 130 140 150
LADCGIGFGK NAIGGRDGEI YVVTDPGNDD PVNPRPGTLR YAVIQDEPLW
160 170 180 190 200
IIFKRDMTIQ LKEELIMNSF KTLDGRGASV HISGGPCITI QYVTNIIIHG
210 220 230 240 250
LHIHDCKQGG NTYVRDSPEH YGYRTVSDGD GVSIFGGSHV WVDHCSLSNC
260 270 280 290 300
NDGLIDAIRG STAITISNNY LTHHNKVMLL GHSDTYEQDK NMQVTIAFNH
310 320 330 340 350
FGEGLVQRMP RCRHGYFHVV NNDYTHWEMY AIGGSANPTI NSQGNRFLAP
360 370 380 390 400
DDSSSKEVTK HEDAPEDEWR NWNWRSEGDL LLNGAFFTYS GAGPAKSSSY
410 420 430
SKASSLAARP SSHVGEITIA SGALSCKRGS HC
Sequence cautioni
The sequence BAB10560 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB008265 Genomic DNA Translation: BAB10560.1 Different initiation. CP002688 Genomic DNA Translation: AED97715.1 AY058197 mRNA Translation: AAL25610.1 |
RefSeqi | NP_568967.1, NM_125713.3 |
Genome annotation databases
EnsemblPlantsi | AT5G63180.1; AT5G63180.1; AT5G63180 |
GeneIDi | 836439 |
Gramenei | AT5G63180.1; AT5G63180.1; AT5G63180 |
KEGGi | ath:AT5G63180 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB008265 Genomic DNA Translation: BAB10560.1 Different initiation. CP002688 Genomic DNA Translation: AED97715.1 AY058197 mRNA Translation: AAL25610.1 |
RefSeqi | NP_568967.1, NM_125713.3 |
3D structure databases
SMRi | Q93Z25 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G63180.1 |
Protein family/group databases
CAZyi | PL1, Polysaccharide Lyase Family 1 |
Proteomic databases
PaxDbi | Q93Z25 |
PRIDEi | Q93Z25 |
ProteomicsDBi | 234676 |
Genome annotation databases
EnsemblPlantsi | AT5G63180.1; AT5G63180.1; AT5G63180 |
GeneIDi | 836439 |
Gramenei | AT5G63180.1; AT5G63180.1; AT5G63180 |
KEGGi | ath:AT5G63180 |
Organism-specific databases
Araporti | AT5G63180 |
TAIRi | locus:2161992, AT5G63180 |
Phylogenomic databases
eggNOGi | ENOG502QQ5F, Eukaryota |
HOGENOMi | CLU_026608_0_1_1 |
InParanoidi | Q93Z25 |
OMAi | CKRGSHC |
OrthoDBi | 924221at2759 |
PhylomeDBi | Q93Z25 |
Enzyme and pathway databases
UniPathwayi | UPA00545;UER00824 |
BioCyci | ARA:AT5G63180-MONOMER |
Miscellaneous databases
PROi | PR:Q93Z25 |
Gene expression databases
ExpressionAtlasi | Q93Z25, baseline and differential |
Genevisiblei | Q93Z25, AT |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR018082, AmbAllergen IPR002022, Pec_lyase IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR045032, PEL |
PANTHERi | PTHR31683, PTHR31683, 1 hit |
Pfami | View protein in Pfam PF00544, Pectate_lyase_4, 1 hit |
PRINTSi | PR00807, AMBALLERGEN |
SMARTi | View protein in SMART SM00656, Amb_all, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLY22_ARATH | |
Accessioni | Q93Z25Primary (citable) accession number: Q93Z25 Secondary accession number(s): Q9FMK5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 16, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families