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Entry version 70 (29 Sep 2021)
Sequence version 1 (01 Dec 2001)
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Protein

DNA-directed RNA polymerase 1, mitochondrial

Gene

RPOT1

Organism
Nicotiana sylvestris (Wood tobacco) (South American tobacco)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei703By similarity1
Active sitei778By similarity1
Active sitei935By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA-directed RNA polymerase 1, mitochondrial (EC:2.7.7.6)
Alternative name(s):
NsRpoT-A
T7 bacteriophage-type single subunit RNA polymerase 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:RPOT1
Synonyms:RPOT-A
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiNicotiana sylvestris (Wood tobacco) (South American tobacco)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri4096 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000189701 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome assembly

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase, Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 21MitochondrionSequence analysisAdd BLAST21
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000003107122 – 1002DNA-directed RNA polymerase 1, mitochondrialAdd BLAST981

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

The highest levels of expression are detected in the mature leaves. The level of expression is lowest in the cotyledons.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
4096.XP_009767249.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q93Y94

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1038, Eukaryota

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.1320.10, 1 hit
1.10.287.260, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR024075, DNA-dir_RNA_pol_helix_hairp_sf
IPR002092, DNA-dir_Rpol_phage-type
IPR043502, DNA/RNA_pol_sf
IPR037159, RNA_POL_N_sf
IPR029262, RPOL_N

The PANTHER Classification System

More...
PANTHERi
PTHR10102, PTHR10102, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00940, RNA_pol, 1 hit
PF14700, RPOL_N, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01311, RPOL_N, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56672, SSF56672, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00900, RNA_POL_PHAGE_1, 1 hit
PS00489, RNA_POL_PHAGE_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q93Y94-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWRYISKQAY SRKFRNSHDS ALLGFSQYSS SFGKTRPLQC LCEESTTNPN
60 70 80 90 100
LGLSQNSIFS RISRKVRHLE GICEESSKNP HLGLSQNSLF SSVKGDFRVC
110 120 130 140 150
GKRGSGSLGF LRSYGSAAEA IASTSEEDID EIQELIEEMN KENEALKTNL
160 170 180 190 200
QPKQPKTIGG MGVGKYNLLR RRQIKVETEA WEEAAKEYQE LLMDMCEQKL
210 220 230 240 250
APNLPYMKSL FLGWFEPLRD AIAAEQKLCD EGKNRGAYAP FFDQLPAEMM
260 270 280 290 300
AVITMHKLMG LLMTGGGTGS ARVVQAASHI GEAIEHEARI HRFLEKTKKS
310 320 330 340 350
NALSGDLEDT PGDIMKERER VRKKVKILMK KQKLQQVRKI VKQQDDEKPW
360 370 380 390 400
GQDNLVKVGC RLIQILMETA YIQPPNDQLD DCPPDIRPAF VHTLKTVETM
410 420 430 440 450
KGSRRYGVIQ CDPLVRKGLD KTARHMVIPY MPMLVPPQSW LGYDKGAYLF
460 470 480 490 500
LPSYIMRTHG AKQQREAVKR VPKKQLEPVF QALDTLGNTK WRLNRKVLGI
510 520 530 540 550
VDRIWASGGR LADLVDREDV PLPEEPDAED EAQIRKWKWK VKGVKKENCE
560 570 580 590 600
RHSQRCDIEL KLAVARKMKD EDGFYYPHNL DFRGRAYPMH PYLNHLGSDL
610 620 630 640 650
CRGILEFAEG RPLGKSGLRW LKIHLANVYG GGVDKLSYEG RVAFSENHVE
660 670 680 690 700
DIFDSAERPL EGKRWWLGAE DPFQCLATCI NIAEALRSPS PETAISYMPI
710 720 730 740 750
HQDGSCNGLQ HYAALGRDTL GAAAVNLVAG DKPADVYSGI AARVLDIMKR
760 770 780 790 800
DAAKDPANDP NVMRARLLIN QVDRKLVKQT VMTSVYGVTY IGARDQIKRR
810 820 830 840 850
LKERGVIEDD NELFAAACYA AKTTLTALGE MFEAARSIMS WLGDCAKIIA
860 870 880 890 900
MENHPVRWTT PLGLPVVQPY RKLGRHLIKT SLQILTLQRE TDKVMVKRQR
910 920 930 940 950
TAFPPNFVHS LDGSHMMMTA IACKESGLSF AGVHDSYWTH ASDVDQMNKI
960 970 980 990 1000
LREKFVELYD APILENLLES FQQSFPDLQF PPLPERGDFD LREVLESPYF

FN
Length:1,002
Mass (Da):113,454
Last modified:December 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i533FA09C13C68804
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti8Q → H in CAC95019 (PubMed:12061895).Curated1
Sequence conflicti129I → T in BAB69063 (PubMed:11722843).Curated1
Sequence conflicti238Y → C in BAB69063 (PubMed:11722843).Curated1
Sequence conflicti261L → W in BAB69063 (PubMed:11722843).Curated1
Sequence conflicti321V → L in CAC95019 (PubMed:12061895).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB058956 Genomic DNA Translation: BAB69431.1
AB058954 mRNA Translation: BAB69063.1
AJ416568 mRNA Translation: CAC95019.1

NCBI Reference Sequences

More...
RefSeqi
NP_001289502.1, NM_001302573.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
104218451

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058956 Genomic DNA Translation: BAB69431.1
AB058954 mRNA Translation: BAB69063.1
AJ416568 mRNA Translation: CAC95019.1
RefSeqiNP_001289502.1, NM_001302573.1

3D structure databases

SMRiQ93Y94
ModBaseiSearch...

Protein-protein interaction databases

STRINGi4096.XP_009767249.1

Genome annotation databases

GeneIDi104218451

Phylogenomic databases

eggNOGiKOG1038, Eukaryota

Family and domain databases

Gene3Di1.10.1320.10, 1 hit
1.10.287.260, 1 hit
InterProiView protein in InterPro
IPR024075, DNA-dir_RNA_pol_helix_hairp_sf
IPR002092, DNA-dir_Rpol_phage-type
IPR043502, DNA/RNA_pol_sf
IPR037159, RNA_POL_N_sf
IPR029262, RPOL_N
PANTHERiPTHR10102, PTHR10102, 1 hit
PfamiView protein in Pfam
PF00940, RNA_pol, 1 hit
PF14700, RPOL_N, 1 hit
SMARTiView protein in SMART
SM01311, RPOL_N, 1 hit
SUPFAMiSSF56672, SSF56672, 1 hit
PROSITEiView protein in PROSITE
PS00900, RNA_POL_PHAGE_1, 1 hit
PS00489, RNA_POL_PHAGE_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRPOT1_NICSY
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q93Y94
Secondary accession number(s): Q8L6K0, Q948R5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: September 29, 2021
This is version 70 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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