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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3

Gene

MNS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Class I alpha-mannosidase essential for early N-glycan processing. Removes preferentially alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man8GlcNAc2. Involved in root development and cell wall biosynthesis.1 Publication

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.1 Publication

Cofactori

Ca2+1 Publication, Mn2+1 Publication, Mg2+1 PublicationNote: Ca2+ or Mn2+. Mg2+ can be used to a lesser extent.1 Publication

Activity regulationi

Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei212Proton donorBy similarity1
Active sitei357By similarity1
Active sitei485Proton donorBy similarity1
Active sitei526By similarity1
Metal bindingi613CalciumBy similarity1

GO - Molecular functioni

  • alpha-mannosidase activity Source: TAIR
  • calcium ion binding Source: InterPro
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: GO_Central

GO - Biological processi

  • N-glycan processing Source: TAIR
  • protein glycosylation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
LigandCalcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-901032 ER Quality Control Compartment (ERQC)
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 (EC:3.2.1.1131 Publication)
Gene namesi
Name:MNS3
Ordered Locus Names:At1g30000
ORF Names:T1P2.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G30000
TAIRilocus:2198299 AT1G30000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43CytoplasmicSequence analysisAdd BLAST43
Transmembranei44 – 64Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini65 – 624LumenalSequence analysisAdd BLAST560

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Formation of aberrant N-glycan structures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003979351 – 624Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3Add BLAST624

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi236N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi377N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi428 ↔ 471By similarity
Glycosylationi503N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ93Y37
PRIDEiQ93Y37

PTM databases

SwissPalmiQ93Y37

Expressioni

Tissue specificityi

Expressed in flowers, siliques, stems, leaves, roots, stamens and sepals.1 Publication

Gene expression databases

ExpressionAtlasiQ93Y37 baseline and differential
GenevisibleiQ93Y37 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G30000.1

Structurei

3D structure databases

ProteinModelPortaliQ93Y37
SMRiQ93Y37
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
HOGENOMiHOG000181987
InParanoidiQ93Y37
KOiK01230
OMAiDHLTCYL
OrthoDBiEOG09360BX2
PhylomeDBiQ93Y37

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q93Y37-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKSLPYSVK DIHYDNAKFR HRSPLKVFSQ SLLTLSTKRN YASCSTGKFL
60 70 80 90 100
ILILFFGVAC LMLMSKSPNE SGLNEKGKVT FVGGLRLGGL LRKPPRLPPR
110 120 130 140 150
LSPDEGQLRG SSTNGSTISN SDPKWAARQQ SVKEAFDHAW SGYRKYAMGY
160 170 180 190 200
DELMPISQKG VDGLGGLGAT VVDALDTAMI MGLDNIVSEA GSWVETHLLE
210 220 230 240 250
RISQKGQVNL FETTIRVLGG LLSAYHLSGG EQGTVNMTHV GPKPVIYLNI
260 270 280 290 300
AKDLADRLLS AFTSSPTPVP FCDVILHEST AHPAPGGASS TAEVASVQLE
310 320 330 340 350
FNYLSSISGD PKYSTEAMKV LAHIKTLPKT EGLVPIYISP QTGDFVGENI
360 370 380 390 400
RLGSRGDSYY EYLIKVWLQQ GAKLNSNFTY LHDMYIEAMK GVRHLLVQNS
410 420 430 440 450
IPKGLVFVGE LPYGSKGEFS PKMDHLVCFL PGTLALGATK GLTKEQALKE
460 470 480 490 500
NLLSFEDLEN LKLAEDLAKT CFEMYEVTAT GLAPEIAYFH TKDYTEDGLD
510 520 530 540 550
GGNKSSMYAN DIIIKPADRH NLLRPETVES LFVLYRITKD TKYRDQGWQI
560 570 580 590 600
FEAFEKYTKV KSGGYTSLDD VTEVPPHRRD KMETFFLGET LKYLYLLFGD
610 620
DSVIPLDKFV FNTEAHPLPI RRNT
Length:624
Mass (Da):69,069
Last modified:December 1, 2001 - v1
Checksum:iA28B82AD7FAAD29D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1P8AM79A0A1P8AM79_ARATH
alpha-1,2-Mannosidase
MNS3 alpha-mannosidase 3, At1g30000, T1P2.10, T1P2_10
644Annotation score:

Sequence cautioni

The sequence AAG52061 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022455 Genomic DNA Translation: AAG52061.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE31166.1
AY054482 mRNA Translation: AAK96673.1
AY093280 mRNA Translation: AAM13279.1
PIRiH86423
RefSeqiNP_564345.1, NM_102740.3
UniGeneiAt.26476

Genome annotation databases

EnsemblPlantsiAT1G30000.1; AT1G30000.1; AT1G30000
GeneIDi839879
GrameneiAT1G30000.1; AT1G30000.1; AT1G30000
KEGGiath:AT1G30000

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022455 Genomic DNA Translation: AAG52061.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE31166.1
AY054482 mRNA Translation: AAK96673.1
AY093280 mRNA Translation: AAM13279.1
PIRiH86423
RefSeqiNP_564345.1, NM_102740.3
UniGeneiAt.26476

3D structure databases

ProteinModelPortaliQ93Y37
SMRiQ93Y37
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G30000.1

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

PTM databases

SwissPalmiQ93Y37

Proteomic databases

PaxDbiQ93Y37
PRIDEiQ93Y37

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G30000.1; AT1G30000.1; AT1G30000
GeneIDi839879
GrameneiAT1G30000.1; AT1G30000.1; AT1G30000
KEGGiath:AT1G30000

Organism-specific databases

AraportiAT1G30000
TAIRilocus:2198299 AT1G30000

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
HOGENOMiHOG000181987
InParanoidiQ93Y37
KOiK01230
OMAiDHLTCYL
OrthoDBiEOG09360BX2
PhylomeDBiQ93Y37

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-ATH-901032 ER Quality Control Compartment (ERQC)

Miscellaneous databases

PROiPR:Q93Y37

Gene expression databases

ExpressionAtlasiQ93Y37 baseline and differential
GenevisibleiQ93Y37 AT

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMNS3_ARATH
AccessioniPrimary (citable) accession number: Q93Y37
Secondary accession number(s): Q9C8R9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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