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Protein

Nicotine blue oxidoreductase

Gene

nboR

Organism
Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of nicotine blue to its hydroquinone form. Nicotine blue is the name given to the compound formed by the autocatalytic condensation of two molecules of 2,3,6-trihydroxypyridine, an intermediate in the nicotine degradation pathway. May play a role in preventing the intracellular formation of nicotine blue semiquinone radicals, which by redox cycling would lead to the formation of toxic reactive oxygen species. Besides nicotine blue, several other quinones are reduced by nboR.1 Publication

Catalytic activityi

3,3'-bipyridine-2,2',5,5',6,6'-hexol + NAD(P)+ = (E)-2,2',5,5'-tetrahydroxy-6H,6'H-(3,3'-bipyridinylidene)-6,6'-dione + NAD(P)H.1 Publication

Cofactori

FMN1 Publication

Kineticsi

  1. KM=6.25 µM for 1,4-naphthoquinone1 Publication
  2. KM=8.83 µM for 1,4-benzoquinone1 Publication
  3. KM=23 µM for 2,6-dichlorophenolindophenol1 Publication

    Pathwayi: nicotine degradation

    This protein is involved in the pathway nicotine degradation, which is part of Alkaloid degradation.
    View all proteins of this organism that are known to be involved in the pathway nicotine degradation and in Alkaloid degradation.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processAlkaloid metabolism
    LigandNADP

    Enzyme and pathway databases

    UniPathwayi
    UPA00106

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotine blue oxidoreductase (EC:1.1.1.328)
    Gene namesi
    Name:nboR
    Encoded oniPlasmid pAO10 Publication
    OrganismiPaenarthrobacter nicotinovorans (Arthrobacter nicotinovorans)
    Taxonomic identifieri29320 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePaenarthrobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004240881 – 204Nicotine blue oxidoreductaseAdd BLAST204

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ93NF6
    SMRiQ93NF6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    KOiK19190

    Family and domain databases

    Gene3Di3.90.550.10, 1 hit
    InterProiView protein in InterPro
    IPR025877 MobA-like_NTP_Trfase
    IPR029044 Nucleotide-diphossugar_trans
    PfamiView protein in Pfam
    PF12804 NTP_transf_3, 1 hit
    SUPFAMiSSF53448 SSF53448, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q93NF6-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MGDTDLPCVT GVLLAAGAGK RLGRGPKALL PYRGRTLVED AAETMLVGGC
    60 70 80 90 100
    HEVVIVLGAN AQAVCARANL EPYRIVVNHD WSSGMGSSYL AGDAAAHTKN
    110 120 130 140 150
    HILVALVDQP GLSVTTVGRL LVSHRPGRIS SAAYSSLDSP RVLRRGHPMV
    160 170 180 190 200
    IDAGLRPAVA STVSGDAGAR VFLRQKPWLV DLIDCSDEST GEDVDTVEQM

    YRLL
    Length:204
    Mass (Da):21,537
    Last modified:December 1, 2001 - v1
    Checksum:i893F0CE27D40CF6B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF373840 Genomic DNA Translation: AAK64261.1
    AJ507836 Genomic DNA Translation: CAD47930.1
    RefSeqiWP_016359441.1, NC_021229.1
    YP_007988756.1, NC_021229.1

    Genome annotation databases

    GeneIDi15879622
    KEGGiag:CAD47930

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF373840 Genomic DNA Translation: AAK64261.1
    AJ507836 Genomic DNA Translation: CAD47930.1
    RefSeqiWP_016359441.1, NC_021229.1
    YP_007988756.1, NC_021229.1

    3D structure databases

    ProteinModelPortaliQ93NF6
    SMRiQ93NF6
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi15879622
    KEGGiag:CAD47930

    Phylogenomic databases

    KOiK19190

    Enzyme and pathway databases

    UniPathwayi
    UPA00106

    Family and domain databases

    Gene3Di3.90.550.10, 1 hit
    InterProiView protein in InterPro
    IPR025877 MobA-like_NTP_Trfase
    IPR029044 Nucleotide-diphossugar_trans
    PfamiView protein in Pfam
    PF12804 NTP_transf_3, 1 hit
    SUPFAMiSSF53448 SSF53448, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNBOR_PAENI
    AccessioniPrimary (citable) accession number: Q93NF6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: December 1, 2001
    Last modified: October 25, 2017
    This is version 50 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
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