UniProtKB - Q93MI2 (DOXA_STRC0)
Cytochrome P-450 monooxygenase DoxA
doxA
Functioni
Involved in the biosynthesis of the anthracyclines carminomycin, daunorubicin (daunomycin) and doxorubicin (adriamycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, DoxA catalyzes the C-13 hydroxylation of 13-deoxycarminomycin and 13-deoxydaunorubicin to yield 13-dihydrocarminomycin and 13-dihydrodaunorubicin, respectively, as well as the oxidation of these 13-dihydro-anthracyclines to their respective 13-keto forms, carminomycin and daunorubicin. In vivo, it also catalyzes the C-14 hydroxylation of daunorubicin to form doxorubicin. It can only use NADP. DoxA acts jointly with DnrV.
2 PublicationsMiscellaneous
Catalytic activityi
- EC:1.14.13.1811 Publication
- EC:1.14.13.1811 Publication
- EC:1.14.13.1811 Publication
Cofactori
: daunorubicin biosynthesis Pathwayi
This protein is involved in the pathway daunorubicin biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway daunorubicin biosynthesis and in Antibiotic biosynthesis.
Pathwayi: carminomycin biosynthesis
This protein is involved in the pathway carminomycin biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway carminomycin biosynthesis and in Antibiotic biosynthesis.
Pathwayi: doxorubicin biosynthesis
This protein is involved in the pathway doxorubicin biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway doxorubicin biosynthesis and in Antibiotic biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 367 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB
GO - Biological processi
- daunorubicin biosynthetic process Source: UniProtKB
- doxorubicin metabolic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Antibiotic biosynthesis |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
UniPathwayi | UPA00054 UPA01040 UPA01041 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P-450 monooxygenase DoxA (EC:1.14.13.1811 Publication)Alternative name(s): 13-deoxycarminomycin C-13 hydroxylase 13-deoxydaunorubicin C-13 hydroxylase 13-dihydrocarminomycin C-13 hydroxylase 13-dihydrodaunorubicin C-13 hydroxylase Daunorubicin C-14 hydroxylase |
Gene namesi | Name:doxA |
Organismi | Streptomyces peucetius subsp. caesius |
Taxonomic identifieri | 55158 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces › |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000425685 | 1 – 420 | Cytochrome P-450 monooxygenase DoxAAdd BLAST | 420 |
Interactioni
Subunit structurei
Monomer.
By similarityFamily & Domainsi
Sequence similaritiesi
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR002397, Cyt_P450_B IPR017972, Cyt_P450_CS IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00359, BP450 PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSGEAPRVAV DPFACPMMTM QRKPEVHDAF REAGPVVEVN APAGGPAWFI
60 70 80 90 100
TDDALSRYVL ADPRLVKDPD LAPAAWRGVV DGLDIPVPEL RPFTLIAVDG
110 120 130 140 150
EAHRRLHRIH APAFNPRRLA ERTDRIAAIA GRLLTELADA SGRSGEPAEL
160 170 180 190 200
IGGFAYHFPL LVICELLGVP VTVPMAREAV SVLKALASAA QSGGGDGTDP
210 220 230 240 250
AGGVPDTSAL ESLLLEAVHS ARRNDTPTMT RVLYEHTQAE FGSVSDNQLV
260 270 280 290 300
YMITGIIFAG HERTGSFLGF LLAEVLAGRL AADADEDAVS RFVEEAVRYH
310 320 330 340 350
PPVPYTLWRF AATEVTIGGV RLPPGAPVLV DIEGTNTDGR HHDAPHAFHP
360 370 380 390 400
DRPSWRRLTF GDGPHYCIGE QLAQLESRTM IGVLRSRFPE ARLAVPYDEL
410 420
RWCRNGAQTA RLTELPVWLR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF403708 Genomic DNA Translation: AAK95626.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF403708 Genomic DNA Translation: AAK95626.1 |
3D structure databases
SMRi | Q93MI2 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00054 UPA01040 UPA01041 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR002397, Cyt_P450_B IPR017972, Cyt_P450_CS IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00359, BP450 PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DOXA_STRC0 | |
Accessioni | Q93MI2Primary (citable) accession number: Q93MI2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 19, 2014 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families