UniProtKB - Q93FG6 (LKB2B_MANHA)
Protein
Leukotoxin translocation ATP-binding protein LktB
Gene
lktB
Organism
Mannheimia haemolytica (Pasteurella haemolytica)
Status
Functioni
Part of the ABC transporter complex LktBD involved in leukotoxin export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (Probable).Curated
Caution
Leu-10 is present instead of the conserved Cys which is expected to be the active site residue of peptidase C39. Thus this protein is presumed to be without peptidase activity.Curated
Catalytic activityi
- EC:7.4.2.5
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 503 – 510 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type protein transporter activity Source: UniProtKB-EC
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- protein secretion by the type I secretion system Source: InterPro
- proteolysis Source: InterPro
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:lktB |
Organismi | Mannheimia haemolytica (Pasteurella haemolytica) |
Taxonomic identifieri | 75985 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia |
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- type I protein secretion system complex Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 159 – 179 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 192 – 212 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 270 – 290 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 296 – 316 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 389 – 409 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092382 | 1 – 708 | Leukotoxin translocation ATP-binding protein LktBAdd BLAST | 708 |
Interactioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 126 | Peptidase C39PROSITE-ProRule annotationAdd BLAST | 126 | |
Domaini | 155 – 437 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 283 | |
Domaini | 469 – 704 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 236 |
Domaini
In LktB the peptidase C39 domain, the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Protein-1 exporter (TC 3.A.1.109) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd02417, Peptidase_C39_likeA, 1 hit |
Gene3Di | 1.20.1560.10, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR010132, ATPase_T1SS_HlyB IPR027417, P-loop_NTPase IPR005074, Peptidase_C39 IPR039395, Peptidase_C39-like_A IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF03412, Peptidase_C39, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01846, type_I_sec_HlyB, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS50990, PEPTIDASE_C39, 1 hit |
i Sequence
Sequence statusi: Complete.
Q93FG6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEANHQRNDL GLVALTMLAQ YHNISLNPEE IKHKFDLDGK GLSLTSWLLA
60 70 80 90 100
AKSLALKAKH IKKEISRLHL VNLPALVWQD NGKHFLLVKV DTDNNRYLTY
110 120 130 140 150
NLEQDAPQIL SQDEFEACYQ GQLILVTSRA SVVGQLAKFD FTWFIPAVIK
160 170 180 190 200
YRKIFLETLI VSIFLQIFAL ITPLFFQVVM DKVLVHRGFS TLNIITVALA
210 220 230 240 250
IVIIFEIVLS GLRTYVFSHS TSRIDVELGA KLFRHLLSLP ISYFENRRVG
260 270 280 290 300
DTVARVRELD QIRNFLTGQA LTSVLDLLFS FIFFAVMWYY SPKLTLVILG
310 320 330 340 350
SLPCYILWSI FISPILRRRL DDKFARSADN QAFLVESVTA INMIKAMAVA
360 370 380 390 400
PQMTDTWDKQ LASYVSSSFR VTVLATIGQQ GVQLIQKTVM VINLWLGAHL
410 420 430 440 450
VISGDLSIGQ LIAFNMLSGQ VIAPVIRLAQ LWQDFQQVGI SVTRLGDVLN
460 470 480 490 500
SPTEQYQGKL SLPEIKGYIS FKNIRFRYKP DAPTILNNVN LEIRQGEVIG
510 520 530 540 550
IVGRSGSGKS TLTKLLQRFY IPENGQVLID GHDLALADPN WLRRQIGVVL
560 570 580 590 600
QDNVLLNRSI RENIALSDPG MPMERVIYAA KLAGAHDFIS ELREGYNTIV
610 620 630 640 650
GEQGAGLSGG QRQRIAIARA LVNNPKILIF DEATSALDYE SEHIIMQNMQ
660 670 680 690 700
KICQGRTVIL IAHRLSTVKN ADRIIVMEKG EIVEQGKHHE LLQNSNGLYS
YLHQLQLN
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 46 | S → A in strain: Serotype A2 / PH278 and Serotype A2 / PH494. | 1 | |
Natural varianti | 65 | I → V in strain: Serotype A2 / PH494. | 1 | |
Natural varianti | 101 | N → D in strain: Serotype A2 / PH494. | 1 | |
Natural varianti | 104 | Q → K in strain: Serotype A2 / PH494. | 1 | |
Natural varianti | 322 | D → E in strain: Serotype A2 / PH278. | 1 | |
Natural varianti | 468 | Y → D in strain: Serotype A2 / PH278 and Serotype A2 / PH494. | 1 | |
Natural varianti | 655 | G → D in strain: Serotype A2 / PH278. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF314511 Genomic DNA Translation: AAL12782.1 AF314514 Genomic DNA Translation: AAL12791.1 AF314515 Genomic DNA Translation: AAL12794.1 |
RefSeqi | WP_020831006.1, NZ_VAIO01000006.1 WP_032844367.1, NZ_VAIL01000117.1 |
Genome annotation databases
PATRICi | fig|75985.47.peg.432 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF314511 Genomic DNA Translation: AAL12782.1 AF314514 Genomic DNA Translation: AAL12791.1 AF314515 Genomic DNA Translation: AAL12794.1 |
RefSeqi | WP_020831006.1, NZ_VAIO01000006.1 WP_032844367.1, NZ_VAIL01000117.1 |
3D structure databases
SMRi | Q93FG6 |
ModBasei | Search... |
Genome annotation databases
PATRICi | fig|75985.47.peg.432 |
Family and domain databases
CDDi | cd02417, Peptidase_C39_likeA, 1 hit |
Gene3Di | 1.20.1560.10, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR010132, ATPase_T1SS_HlyB IPR027417, P-loop_NTPase IPR005074, Peptidase_C39 IPR039395, Peptidase_C39-like_A IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF03412, Peptidase_C39, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01846, type_I_sec_HlyB, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS50990, PEPTIDASE_C39, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LKB2B_MANHA | |
Accessioni | Q93FG6Primary (citable) accession number: Q93FG6 Secondary accession number(s): Q934E0, Q93FG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |