UniProtKB - Q93091 (RNAS6_HUMAN)
Protein
Ribonuclease K6
Gene
RNASE6
Organism
Homo sapiens (Human)
Status
Functioni
Ribonuclease which shows a preference for the pyrimidines uridine and cytosine (PubMed:8836175, PubMed:27013146). Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli (PubMed:25075772, PubMed:27089320). Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria (PubMed:27089320). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (PubMed:27089320).4 Publications
Kineticsi
- KM=5.0 µM for tRNA1 Publication
- KM=2.63 mM for UpA dinucleotide1 Publication
- KM=1.22 mM for CpA dinucleotide1 Publication
- KM=2.06 mM for cytidine 2,3-cyclic phosphate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 24 | Important for bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS)1 Publication | 1 | |
Sitei | 36 | Important for bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS)1 Publication | 1 | |
Active sitei | 38 | Proton acceptorBy similarity | 1 | |
Sitei | 59 | Facilitates cleavage of polynucleotide substrates1 Publication | 1 | |
Sitei | 61 | Critical for catalytic activityBy similarity | 1 | |
Binding sitei | 86 | SubstrateBy similarity | 1 | |
Binding sitei | 105 | SubstrateBy similarity | 1 | |
Active sitei | 145 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- endonuclease activity Source: UniProtKB-KW
- nucleic acid binding Source: InterPro
- ribonuclease activity Source: UniProtKB
GO - Biological processi
- antibacterial humoral response Source: UniProtKB
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
- antimicrobial humoral response Source: Reactome
- defense response Source: ProtInc
- defense response to Gram-negative bacterium Source: UniProtKB
- defense response to Gram-positive bacterium Source: UniProtKB
- defense response to virus Source: UniProtKB
- innate immune response Source: UniProtKB
- RNA catabolic process Source: ProtInc
- RNA phosphodiester bond hydrolysis Source: UniProtKB
Keywordsi
Molecular function | Antibiotic, Antimicrobial, Endonuclease, Hydrolase, Nuclease |
Enzyme and pathway databases
PathwayCommonsi | Q93091 |
Reactomei | R-HSA-6803157, Antimicrobial peptides |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RNASE6 Synonyms:RNS6 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10048, RNASE6 |
MIMi | 601981, gene |
neXtProti | NX_Q93091 |
VEuPathDBi | HostDB:ENSG00000169413.2 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Lysosome
- Lysosome 1 Publication
Other locations
- Cytoplasmic granule 1 Publication
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Lysosome
- lysosome Source: UniProtKB-SubCell
Other locations
- cytoplasmic vesicle Source: UniProtKB
Keywords - Cellular componenti
Lysosome, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | W → A: Moderately impairs bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS). 1 Publication | 1 | |
Mutagenesisi | 36 | I → A: Strongly impairs bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS). 1 Publication | 1 | |
Mutagenesisi | 38 | H → A: Significantly reduced activity towards dinucleotides UpA and CpA. Slightly reduced activity towards polymeric substrates poly(U) and poly(U):poly(A). No effect on bactericidal activity. Significantly reduced activity towards poly(U) and poly(U):poly(A); when associated with R-59. 2 Publications | 1 | |
Mutagenesisi | 59 | H → R: No significant effect on activity towards dinucleotides UpA and CpA. Reduced activity towards polymeric substrates poly(U) and poly(U):poly(A). Significantly reduced activity towards poly(U) and poly(U):poly(A); when associated with A-38. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6039 |
OpenTargetsi | ENSG00000169413 |
PharmGKBi | PA34416 |
Miscellaneous databases
Pharosi | Q93091, Tbio |
Genetic variation databases
BioMutai | RNASE6 |
DMDMi | 3123285 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | By similarityAdd BLAST | 23 | |
ChainiPRO_0000030892 | 24 – 150 | Ribonuclease K6Add BLAST | 127 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 ↔ 104 | Combined sources | ||
Glycosylationi | 55 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 60 ↔ 114 | Combined sources | ||
Disulfide bondi | 78 ↔ 129 | Combined sources | ||
Disulfide bondi | 85 ↔ 92 | Combined sources | ||
Glycosylationi | 100 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q93091 |
jPOSTi | Q93091 |
MassIVEi | Q93091 |
PaxDbi | Q93091 |
PeptideAtlasi | Q93091 |
PRIDEi | Q93091 |
ProteomicsDBi | 75720 |
PTM databases
GlyGeni | Q93091, 2 sites |
Expressioni
Tissue specificityi
Highly expressed in spleen (at protein level) (PubMed:8836175, PubMed:25075772). Has little or no expression in healthy kidneys (at protein level) (PubMed:25075772). Detected in interstitial leukocytes in infected kidneys (at protein level) (PubMed:25075772). Expressed in ureter where it localizes to urothelial and submucosal leukocytes (at protein level) (PubMed:25075772). Strong expression in lung and thymus, and lower expression in heart, placenta, pancreas, liver, brain and skeletal muscle (PubMed:8836175, PubMed:25075772). Also expressed in monocytes and neutrophils (PubMed:8836175).2 Publications
Inductioni
Up-regulated in CD14+ monocytes in response to the uropathogenic E.coli strain CFT073.1 Publication
Gene expression databases
Bgeei | ENSG00000169413, Expressed in leukocyte and 184 other tissues |
ExpressionAtlasi | Q93091, baseline and differential |
Genevisiblei | Q93091, HS |
Organism-specific databases
HPAi | ENSG00000169413, Tissue enhanced (blood) |
Interactioni
Subunit structurei
Interacts (via N-terminus) with bacterial lipopolysaccharide (LPS).
1 PublicationProtein-protein interaction databases
BioGRIDi | 111968, 1 interactor |
IntActi | Q93091, 1 interactor |
STRINGi | 9606.ENSP00000302046 |
Miscellaneous databases
RNActi | Q93091, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q93091 |
SMRi | Q93091 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 61 – 65 | Substrate bindingBy similarity | 5 |
Sequence similaritiesi
Belongs to the pancreatic ribonuclease family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502TDZ3, Eukaryota |
GeneTreei | ENSGT00940000161733 |
HOGENOMi | CLU_117006_0_1_1 |
InParanoidi | Q93091 |
OMAi | TKAHWFE |
OrthoDBi | 1549558at2759 |
PhylomeDBi | Q93091 |
TreeFami | TF333393 |
Family and domain databases
Gene3Di | 3.10.130.10, 1 hit |
InterProi | View protein in InterPro IPR001427, RNaseA IPR036816, RNaseA-like_dom_sf IPR023411, RNaseA_AS IPR023412, RNaseA_domain |
PANTHERi | PTHR11437, PTHR11437, 1 hit |
Pfami | View protein in Pfam PF00074, RnaseA, 1 hit |
PRINTSi | PR00794, RIBONUCLEASE |
SMARTi | View protein in SMART SM00092, RNAse_Pc, 1 hit |
SUPFAMi | SSF54076, SSF54076, 1 hit |
PROSITEi | View protein in PROSITE PS00127, RNASE_PANCREATIC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q93091-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLCFPLLLL LLVLWGPVCP LHAWPKRLTK AHWFEIQHIQ PSPLQCNRAM
60 70 80 90 100
SGINNYTQHC KHQNTFLHDS FQNVAAVCDL LSIVCKNRRH NCHQSSKPVN
110 120 130 140 150
MTDCRLTSGK YPQCRYSAAA QYKFFIVACD PPQKSDPPYK LVPVHLDSIL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_012048 | 89 | R → Q. Corresponds to variant dbSNP:rs1045922Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U64998 Genomic DNA Translation: AAC51848.1 BC020848 mRNA Translation: AAH20848.1 |
CCDSi | CCDS9558.1 |
PIRi | S72361 |
RefSeqi | NP_005606.1, NM_005615.4 XP_016877055.1, XM_017021566.1 XP_016877056.1, XM_017021567.1 |
Genome annotation databases
Ensembli | ENST00000304677; ENSP00000302046; ENSG00000169413 |
GeneIDi | 6039 |
KEGGi | hsa:6039 |
UCSCi | uc001vye.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U64998 Genomic DNA Translation: AAC51848.1 BC020848 mRNA Translation: AAH20848.1 |
CCDSi | CCDS9558.1 |
PIRi | S72361 |
RefSeqi | NP_005606.1, NM_005615.4 XP_016877055.1, XM_017021566.1 XP_016877056.1, XM_017021567.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4X09 | X-ray | 1.72 | A | 24-150 | [»] | |
5OAB | X-ray | 1.11 | A | 24-150 | [»] | |
6ENP | X-ray | 1.04 | A | 24-150 | [»] | |
6MV6 | X-ray | 1.50 | A | 26-150 | [»] | |
6MV7 | X-ray | 2.59 | A | 24-150 | [»] | |
BMRBi | Q93091 | |||||
SMRi | Q93091 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111968, 1 interactor |
IntActi | Q93091, 1 interactor |
STRINGi | 9606.ENSP00000302046 |
PTM databases
GlyGeni | Q93091, 2 sites |
Genetic variation databases
BioMutai | RNASE6 |
DMDMi | 3123285 |
Proteomic databases
EPDi | Q93091 |
jPOSTi | Q93091 |
MassIVEi | Q93091 |
PaxDbi | Q93091 |
PeptideAtlasi | Q93091 |
PRIDEi | Q93091 |
ProteomicsDBi | 75720 |
Protocols and materials databases
Antibodypediai | 3, 60 antibodies |
DNASUi | 6039 |
Genome annotation databases
Ensembli | ENST00000304677; ENSP00000302046; ENSG00000169413 |
GeneIDi | 6039 |
KEGGi | hsa:6039 |
UCSCi | uc001vye.4, human |
Organism-specific databases
CTDi | 6039 |
DisGeNETi | 6039 |
GeneCardsi | RNASE6 |
HGNCi | HGNC:10048, RNASE6 |
HPAi | ENSG00000169413, Tissue enhanced (blood) |
MIMi | 601981, gene |
neXtProti | NX_Q93091 |
OpenTargetsi | ENSG00000169413 |
PharmGKBi | PA34416 |
VEuPathDBi | HostDB:ENSG00000169413.2 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502TDZ3, Eukaryota |
GeneTreei | ENSGT00940000161733 |
HOGENOMi | CLU_117006_0_1_1 |
InParanoidi | Q93091 |
OMAi | TKAHWFE |
OrthoDBi | 1549558at2759 |
PhylomeDBi | Q93091 |
TreeFami | TF333393 |
Enzyme and pathway databases
PathwayCommonsi | Q93091 |
Reactomei | R-HSA-6803157, Antimicrobial peptides |
Miscellaneous databases
BioGRID-ORCSi | 6039, 5 hits in 873 CRISPR screens |
GenomeRNAii | 6039 |
Pharosi | Q93091, Tbio |
PROi | PR:Q93091 |
RNActi | Q93091, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169413, Expressed in leukocyte and 184 other tissues |
ExpressionAtlasi | Q93091, baseline and differential |
Genevisiblei | Q93091, HS |
Family and domain databases
Gene3Di | 3.10.130.10, 1 hit |
InterProi | View protein in InterPro IPR001427, RNaseA IPR036816, RNaseA-like_dom_sf IPR023411, RNaseA_AS IPR023412, RNaseA_domain |
PANTHERi | PTHR11437, PTHR11437, 1 hit |
Pfami | View protein in Pfam PF00074, RnaseA, 1 hit |
PRINTSi | PR00794, RIBONUCLEASE |
SMARTi | View protein in SMART SM00092, RNAse_Pc, 1 hit |
SUPFAMi | SSF54076, SSF54076, 1 hit |
PROSITEi | View protein in PROSITE PS00127, RNASE_PANCREATIC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNAS6_HUMAN | |
Accessioni | Q93091Primary (citable) accession number: Q93091 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families