RNASE6

Functionⁱ

Ribonuclease which shows a preference for the pyrimidines uridine and cytosine (PubMed:8836175, PubMed:27013146). Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli (PubMed:25075772, PubMed:27089320). Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria (PubMed:27089320). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (PubMed:27089320).4 Publications

Kineticsⁱ

Sites

Feature key	Position(s)	DescriptionActions	Length
Siteⁱ	24	Important for bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS)1 Publication	1
Siteⁱ	36	Important for bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS)1 Publication	1
Active siteⁱ	38	Proton acceptorBy similarity	1
Siteⁱ	59	Facilitates cleavage of polynucleotide substrates1 Publication	1
Siteⁱ	61	Critical for catalytic activityBy similarity	1
Binding siteⁱ	86	SubstrateBy similarity	1
Binding siteⁱ	105	SubstrateBy similarity	1
Active siteⁱ	145	Proton donorBy similarity	1

GO - Molecular functionⁱ

endonuclease activity Source: UniProtKB-KW
nucleic acid binding Source: InterPro
ribonuclease activity
Source: UniProtKB
Inferred from direct assayⁱ
- 9826755

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

antibacterial humoral response
Source: UniProtKB
Inferred from direct assayⁱ
- 25075772
antimicrobial humoral immune response mediated by antimicrobial peptide
Source: UniProtKB
Inferred from direct assayⁱ
- 25075772
antimicrobial humoral response Source: Reactome
defense response
Source: ProtInc
Traceable author statementⁱ
- 8836175
defense response to Gram-negative bacterium
Source: UniProtKB
Inferred from direct assayⁱ
- 25075772
defense response to Gram-positive bacterium
Source: UniProtKB
Inferred from direct assayⁱ
- 25075772
defense response to virus
Source: UniProtKB
Inferred from direct assayⁱ
- 9826755
innate immune response
Source: UniProtKB
Inferred from direct assayⁱ
- 25075772
RNA catabolic process
Source: ProtInc
Traceable author statementⁱ
- 8836175
RNA phosphodiester bond hydrolysis
Source: UniProtKB
Inferred from direct assayⁱ
- 9826755

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Antibiotic, Antimicrobial, Endonuclease, Hydrolase, Nuclease

Molecular function

Antibiotic, Antimicrobial, Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

Reactomeⁱ	R-HSA-6803157 Antimicrobial peptides

Reactomeⁱ

R-HSA-6803157 Antimicrobial peptides

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ribonuclease K6 (EC:3.1.27.-2 Publications) Short name: RNase K6
Gene namesⁱ	Name:RNASE6 Synonyms:RNS6
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 14

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000169413.2
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:10048 RNASE6
MIMⁱ	601981 gene
neXtProtⁱ	NX_Q93091

Keywords - Cellular componentⁱ

Lysosome, Secreted

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	24	W → A: Moderately impairs bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS). 1 Publication	1
Mutagenesisⁱ	36	I → A: Strongly impairs bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS). 1 Publication	1
Mutagenesisⁱ	38	H → A: Significantly reduced activity towards dinucleotides UpA and CpA. Slightly reduced activity towards polymeric substrates poly(U) and poly(U):poly(A). No effect on bactericidal activity. Significantly reduced activity towards poly(U) and poly(U):poly(A); when associated with R-59. 2 Publications	1
Mutagenesisⁱ	59	H → R: No significant effect on activity towards dinucleotides UpA and CpA. Reduced activity towards polymeric substrates poly(U) and poly(U):poly(A). Significantly reduced activity towards poly(U) and poly(U):poly(A); when associated with A-38. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	6039
Open Targets More...OpenTargetsⁱ	ENSG00000169413
PharmGKBⁱ	PA34416

Polymorphism and mutation databases

BioMutaⁱ	RNASE6
DMDMⁱ	3123285

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 23	By similarityAdd BLAST		23
ChainⁱPRO_0000030892	24 – 150	Ribonuclease K6Add BLAST		127

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	46 ↔ 104	Combined sources
Glycosylationⁱ	55	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	60 ↔ 114	Combined sources
Disulfide bondⁱ	78 ↔ 129	Combined sources
Disulfide bondⁱ	85 ↔ 92	Combined sources
Glycosylationⁱ	100	N-linked (GlcNAc...) asparagineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

EPDⁱ	Q93091
PaxDbⁱ	Q93091
PeptideAtlas More...PeptideAtlasⁱ	Q93091
PRIDEⁱ	Q93091
ProteomicsDBⁱ	75720

Expressionⁱ

Tissue specificityⁱ

Highly expressed in spleen (at protein level) (PubMed:8836175, PubMed:25075772). Has little or no expression in healthy kidneys (at protein level) (PubMed:25075772). Detected in interstitial leukocytes in infected kidneys (at protein level) (PubMed:25075772). Expressed in ureter where it localizes to urothelial and submucosal leukocytes (at protein level) (PubMed:25075772). Strong expression in lung and thymus, and lower expression in heart, placenta, pancreas, liver, brain and skeletal muscle (PubMed:8836175, PubMed:25075772). Also expressed in monocytes and neutrophils (PubMed:8836175).2 Publications

Inductionⁱ

Up-regulated in CD14+ monocytes in response to the uropathogenic E.coli strain CFT073.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000169413 Expressed in 168 organ(s), highest expression level in leukocyte
CleanExⁱ	HS_RNASE6
ExpressionAtlasⁱ	Q93091 baseline and differential
Genevisibleⁱ	Q93091 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA000698

HPAⁱ

HPA000698

Interactionⁱ

Subunit structureⁱ

Interacts (via N-terminus) with bacterial lipopolysaccharide (LPS).1 Publication

Protein-protein interaction databases

BioGridⁱ	111968, 1 interactor
IntActⁱ	Q93091, 1 interactor
STRINGⁱ	9606.ENSP00000302046

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	30 – 38	Combined sources	9
Helixⁱ	46 – 57	Combined sources	12
Beta strandⁱ	62 – 67	Combined sources	6
Helixⁱ	71 – 77	Combined sources	7
Beta strandⁱ	92 – 94	Combined sources	3
Beta strandⁱ	99 – 108	Combined sources	10
Beta strandⁱ	115 – 130	Combined sources	16
Beta strandⁱ	142 – 150	Combined sources	9

Show more details

3D structure databases

ProteinModelPortalⁱ	Q93091
SMRⁱ	Q93091
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	61 – 65	Substrate bindingBy similarity		5

Sequence similaritiesⁱ

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG410IYEW Eukaryota ENOG4111D8Q LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140993
HOGENOMⁱ	HOG000276882
HOVERGENⁱ	HBG008396
InParanoidⁱ	Q93091
KEGG Orthology (KO) More...KOⁱ	K01172
OMAⁱ	HWFEIQH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0U42
PhylomeDBⁱ	Q93091
TreeFamⁱ	TF333393

Family and domain databases

Gene3Dⁱ	3.10.130.10, 1 hit
InterProⁱ	View protein in InterPro IPR001427 RNaseA IPR036816 RNaseA-like_dom_sf IPR023411 RNaseA_AS IPR023412 RNaseA_domain
PANTHERⁱ	PTHR11437 PTHR11437, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00074 RnaseA, 1 hit
PRINTSⁱ	PR00794 RIBONUCLEASE
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD000535 RNaseA, 1 hit
SMARTⁱ	View protein in SMART SM00092 RNAse_Pc, 1 hit
SUPFAMⁱ	SSF54076 SSF54076, 1 hit
PROSITEⁱ	View protein in PROSITE PS00127 RNASE_PANCREATIC, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q93091-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVLCFPLLLL LLVLWGPVCP LHAWPKRLTK AHWFEIQHIQ PSPLQCNRAM 
        60         70         80         90        100
SGINNYTQHC KHQNTFLHDS FQNVAAVCDL LSIVCKNRRH NCHQSSKPVN 
       110        120        130        140        150
MTDCRLTSGK YPQCRYSAAA QYKFFIVACD PPQKSDPPYK LVPVHLDSIL

Length:150

Mass (Da):17,196

Last modified:July 15, 1998 - v2

Checksum:ⁱ4C1F722066BCC13A

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_012048	89	R → Q. Corresponds to variant dbSNP:rs1045922Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U64998 Genomic DNA Translation: AAC51848.1 BC020848 mRNA Translation: AAH20848.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS9558.1
PIRⁱ	S72361
NCBI Reference Sequences More...RefSeqⁱ	NP_005606.1, NM_005615.4 XP_016877055.1, XM_017021566.1 XP_016877056.1, XM_017021567.1
UniGeneⁱ	Hs.23262

Genome annotation databases

Ensemblⁱ	ENST00000304677; ENSP00000302046; ENSG00000169413
GeneIDⁱ	6039
KEGGⁱ	hsa:6039
UCSC genome browser More...UCSCⁱ	uc001vye.4 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q93091	RNASE6 protein		HUMAN		150	UniRef100_Q93091

Protein	Similar proteins		Species	Score	Length	Source
Q93091	Ribonuclease K6		CHLAE		150	UniRef90_Q93091
Ribonuclease K6		GORGO		150
Ribonuclease K6		MACMU		150
Ribonuclease K6		MIOTA		150
Ribonuclease K6		PANTR		150
+21

Protein	Similar proteins		Species	Score	Length	Source
Q93091	Ribonuclease K6		MACMU		150	UniRef50_Q93091
Ribonuclease K6		GORGO		150
Ribonuclease K6		CHLAE		150
Ribonuclease K6		PAPHA		150
Ribonuclease K6		PONPY		150
+39

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U64998 Genomic DNA Translation: AAC51848.1 BC020848 mRNA Translation: AAH20848.1
CCDSⁱ	CCDS9558.1
PIRⁱ	S72361
RefSeqⁱ	NP_005606.1, NM_005615.4 XP_016877055.1, XM_017021566.1 XP_016877056.1, XM_017021567.1
UniGeneⁱ	Hs.23262

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4X09	X-ray	1.72	A	24-150	[»]
	5OAB	X-ray	1.11	A	24-150	[»]
ProteinModelPortalⁱ	Q93091
SMRⁱ	Q93091
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	111968, 1 interactor
IntActⁱ	Q93091, 1 interactor
STRINGⁱ	9606.ENSP00000302046

Polymorphism and mutation databases

BioMutaⁱ	RNASE6
DMDMⁱ	3123285

Proteomic databases

EPDⁱ	Q93091
PaxDbⁱ	Q93091
PeptideAtlasⁱ	Q93091
PRIDEⁱ	Q93091
ProteomicsDBⁱ	75720

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	6039
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000304677; ENSP00000302046; ENSG00000169413
GeneIDⁱ	6039
KEGGⁱ	hsa:6039
UCSCⁱ	uc001vye.4 human

Organism-specific databases

CTDⁱ	6039
DisGeNETⁱ	6039
EuPathDBⁱ	HostDB:ENSG00000169413.2
GeneCardsⁱ	RNASE6
HGNCⁱ	HGNC:10048 RNASE6
HPAⁱ	HPA000698
MIMⁱ	601981 gene
neXtProtⁱ	NX_Q93091
OpenTargetsⁱ	ENSG00000169413
PharmGKBⁱ	PA34416
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IYEW Eukaryota ENOG4111D8Q LUCA
GeneTreeⁱ	ENSGT00900000140993
HOGENOMⁱ	HOG000276882
HOVERGENⁱ	HBG008396
InParanoidⁱ	Q93091
KOⁱ	K01172
OMAⁱ	HWFEIQH
OrthoDBⁱ	EOG091G0U42
PhylomeDBⁱ	Q93091
TreeFamⁱ	TF333393

Enzyme and pathway databases

Reactomeⁱ	R-HSA-6803157 Antimicrobial peptides

Reactomeⁱ

R-HSA-6803157 Antimicrobial peptides

Miscellaneous databases

GenomeRNAiⁱ	6039
Protein Ontology More...PROⁱ	PR:Q93091
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000169413 Expressed in 168 organ(s), highest expression level in leukocyte
CleanExⁱ	HS_RNASE6
ExpressionAtlasⁱ	Q93091 baseline and differential
Genevisibleⁱ	Q93091 HS

Family and domain databases

Gene3Dⁱ	3.10.130.10, 1 hit
InterProⁱ	View protein in InterPro IPR001427 RNaseA IPR036816 RNaseA-like_dom_sf IPR023411 RNaseA_AS IPR023412 RNaseA_domain
PANTHERⁱ	PTHR11437 PTHR11437, 1 hit
Pfamⁱ	View protein in Pfam PF00074 RnaseA, 1 hit
PRINTSⁱ	PR00794 RIBONUCLEASE
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD000535 RNaseA, 1 hit
SMARTⁱ	View protein in SMART SM00092 RNAse_Pc, 1 hit
SUPFAMⁱ	SSF54076 SSF54076, 1 hit
PROSITEⁱ	View protein in PROSITE PS00127 RNASE_PANCREATIC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RNAS6_HUMAN
Accessionⁱ	Q93091Primary (citable) accession number: Q93091
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	July 15, 1998
	Last modified:	November 7, 2018
	This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM

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UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q93091 (RNAS6_HUMAN)

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Ribonuclease K6

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Lysosome

Other locations

Extracellular region or secreted

Lysosome

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

Kineticsⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ