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Protein

Ribonuclease K6

Gene

RNASE6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ribonuclease which shows a preference for the pyrimidines uridine and cytosine (PubMed:8836175, PubMed:27013146). Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli (PubMed:25075772, PubMed:27089320). Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria (PubMed:27089320). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (PubMed:27089320).4 Publications

Kineticsi

  1. KM=5.0 µM for tRNA1 Publication
  2. KM=2.63 mM for UpA dinucleotide1 Publication
  3. KM=1.22 mM for CpA dinucleotide1 Publication
  4. KM=2.06 mM for cytidine 2,3-cyclic phosphate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei24Important for bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS)1 Publication1
    Sitei36Important for bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS)1 Publication1
    Active sitei38Proton acceptorBy similarity1
    Sitei59Facilitates cleavage of polynucleotide substrates1 Publication1
    Sitei61Critical for catalytic activityBy similarity1
    Binding sitei86SubstrateBy similarity1
    Binding sitei105SubstrateBy similarity1
    Active sitei145Proton donorBy similarity1

    GO - Molecular functioni

    • endonuclease activity Source: UniProtKB-KW
    • nucleic acid binding Source: InterPro
    • ribonuclease activity Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionAntibiotic, Antimicrobial, Endonuclease, Hydrolase, Nuclease

    Enzyme and pathway databases

    ReactomeiR-HSA-6803157 Antimicrobial peptides

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease K6 (EC:3.1.27.-2 Publications)
    Short name:
    RNase K6
    Gene namesi
    Name:RNASE6
    Synonyms:RNS6
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 14

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000169413.2
    HGNCiHGNC:10048 RNASE6
    MIMi601981 gene
    neXtProtiNX_Q93091

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Lysosome, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi24W → A: Moderately impairs bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS). 1 Publication1
    Mutagenesisi36I → A: Strongly impairs bactericidal activity, bacterial agglutination activity and binding to bacterial lipopolysaccharide (LPS). 1 Publication1
    Mutagenesisi38H → A: Significantly reduced activity towards dinucleotides UpA and CpA. Slightly reduced activity towards polymeric substrates poly(U) and poly(U):poly(A). No effect on bactericidal activity. Significantly reduced activity towards poly(U) and poly(U):poly(A); when associated with R-59. 2 Publications1
    Mutagenesisi59H → R: No significant effect on activity towards dinucleotides UpA and CpA. Reduced activity towards polymeric substrates poly(U) and poly(U):poly(A). Significantly reduced activity towards poly(U) and poly(U):poly(A); when associated with A-38. 1 Publication1

    Organism-specific databases

    DisGeNETi6039
    OpenTargetsiENSG00000169413
    PharmGKBiPA34416

    Polymorphism and mutation databases

    BioMutaiRNASE6
    DMDMi3123285

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 23By similarityAdd BLAST23
    ChainiPRO_000003089224 – 150Ribonuclease K6Add BLAST127

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi46 ↔ 104Combined sources
    Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi60 ↔ 114Combined sources
    Disulfide bondi78 ↔ 129Combined sources
    Disulfide bondi85 ↔ 92Combined sources
    Glycosylationi100N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    EPDiQ93091
    PaxDbiQ93091
    PeptideAtlasiQ93091
    PRIDEiQ93091
    ProteomicsDBi75720

    Expressioni

    Tissue specificityi

    Highly expressed in spleen (at protein level) (PubMed:8836175, PubMed:25075772). Has little or no expression in healthy kidneys (at protein level) (PubMed:25075772). Detected in interstitial leukocytes in infected kidneys (at protein level) (PubMed:25075772). Expressed in ureter where it localizes to urothelial and submucosal leukocytes (at protein level) (PubMed:25075772). Strong expression in lung and thymus, and lower expression in heart, placenta, pancreas, liver, brain and skeletal muscle (PubMed:8836175, PubMed:25075772). Also expressed in monocytes and neutrophils (PubMed:8836175).2 Publications

    Inductioni

    Up-regulated in CD14+ monocytes in response to the uropathogenic E.coli strain CFT073.1 Publication

    Gene expression databases

    BgeeiENSG00000169413 Expressed in 168 organ(s), highest expression level in leukocyte
    CleanExiHS_RNASE6
    ExpressionAtlasiQ93091 baseline and differential
    GenevisibleiQ93091 HS

    Organism-specific databases

    HPAiHPA000698

    Interactioni

    Subunit structurei

    Interacts (via N-terminus) with bacterial lipopolysaccharide (LPS).1 Publication

    Protein-protein interaction databases

    BioGridi111968, 1 interactor
    IntActiQ93091, 1 interactor
    STRINGi9606.ENSP00000302046

    Structurei

    Secondary structure

    1150
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ93091
    SMRiQ93091
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni61 – 65Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the pancreatic ribonuclease family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IYEW Eukaryota
    ENOG4111D8Q LUCA
    GeneTreeiENSGT00900000140993
    HOGENOMiHOG000276882
    HOVERGENiHBG008396
    InParanoidiQ93091
    KOiK01172
    OMAiHWFEIQH
    OrthoDBiEOG091G0U42
    PhylomeDBiQ93091
    TreeFamiTF333393

    Family and domain databases

    Gene3Di3.10.130.10, 1 hit
    InterProiView protein in InterPro
    IPR001427 RNaseA
    IPR036816 RNaseA-like_dom_sf
    IPR023411 RNaseA_AS
    IPR023412 RNaseA_domain
    PANTHERiPTHR11437 PTHR11437, 1 hit
    PfamiView protein in Pfam
    PF00074 RnaseA, 1 hit
    PRINTSiPR00794 RIBONUCLEASE
    ProDomiView protein in ProDom or Entries sharing at least one domain
    PD000535 RNaseA, 1 hit
    SMARTiView protein in SMART
    SM00092 RNAse_Pc, 1 hit
    SUPFAMiSSF54076 SSF54076, 1 hit
    PROSITEiView protein in PROSITE
    PS00127 RNASE_PANCREATIC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q93091-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVLCFPLLLL LLVLWGPVCP LHAWPKRLTK AHWFEIQHIQ PSPLQCNRAM
    60 70 80 90 100
    SGINNYTQHC KHQNTFLHDS FQNVAAVCDL LSIVCKNRRH NCHQSSKPVN
    110 120 130 140 150
    MTDCRLTSGK YPQCRYSAAA QYKFFIVACD PPQKSDPPYK LVPVHLDSIL
    Length:150
    Mass (Da):17,196
    Last modified:July 15, 1998 - v2
    Checksum:i4C1F722066BCC13A
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_01204889R → Q. Corresponds to variant dbSNP:rs1045922Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U64998 Genomic DNA Translation: AAC51848.1
    BC020848 mRNA Translation: AAH20848.1
    CCDSiCCDS9558.1
    PIRiS72361
    RefSeqiNP_005606.1, NM_005615.4
    XP_016877055.1, XM_017021566.1
    XP_016877056.1, XM_017021567.1
    UniGeneiHs.23262

    Genome annotation databases

    EnsembliENST00000304677; ENSP00000302046; ENSG00000169413
    GeneIDi6039
    KEGGihsa:6039
    UCSCiuc001vye.4 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U64998 Genomic DNA Translation: AAC51848.1
    BC020848 mRNA Translation: AAH20848.1
    CCDSiCCDS9558.1
    PIRiS72361
    RefSeqiNP_005606.1, NM_005615.4
    XP_016877055.1, XM_017021566.1
    XP_016877056.1, XM_017021567.1
    UniGeneiHs.23262

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4X09X-ray1.72A24-150[»]
    5OABX-ray1.11A24-150[»]
    ProteinModelPortaliQ93091
    SMRiQ93091
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi111968, 1 interactor
    IntActiQ93091, 1 interactor
    STRINGi9606.ENSP00000302046

    Polymorphism and mutation databases

    BioMutaiRNASE6
    DMDMi3123285

    Proteomic databases

    EPDiQ93091
    PaxDbiQ93091
    PeptideAtlasiQ93091
    PRIDEiQ93091
    ProteomicsDBi75720

    Protocols and materials databases

    DNASUi6039
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000304677; ENSP00000302046; ENSG00000169413
    GeneIDi6039
    KEGGihsa:6039
    UCSCiuc001vye.4 human

    Organism-specific databases

    CTDi6039
    DisGeNETi6039
    EuPathDBiHostDB:ENSG00000169413.2
    GeneCardsiRNASE6
    HGNCiHGNC:10048 RNASE6
    HPAiHPA000698
    MIMi601981 gene
    neXtProtiNX_Q93091
    OpenTargetsiENSG00000169413
    PharmGKBiPA34416
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiENOG410IYEW Eukaryota
    ENOG4111D8Q LUCA
    GeneTreeiENSGT00900000140993
    HOGENOMiHOG000276882
    HOVERGENiHBG008396
    InParanoidiQ93091
    KOiK01172
    OMAiHWFEIQH
    OrthoDBiEOG091G0U42
    PhylomeDBiQ93091
    TreeFamiTF333393

    Enzyme and pathway databases

    ReactomeiR-HSA-6803157 Antimicrobial peptides

    Miscellaneous databases

    GenomeRNAii6039
    PROiPR:Q93091
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000169413 Expressed in 168 organ(s), highest expression level in leukocyte
    CleanExiHS_RNASE6
    ExpressionAtlasiQ93091 baseline and differential
    GenevisibleiQ93091 HS

    Family and domain databases

    Gene3Di3.10.130.10, 1 hit
    InterProiView protein in InterPro
    IPR001427 RNaseA
    IPR036816 RNaseA-like_dom_sf
    IPR023411 RNaseA_AS
    IPR023412 RNaseA_domain
    PANTHERiPTHR11437 PTHR11437, 1 hit
    PfamiView protein in Pfam
    PF00074 RnaseA, 1 hit
    PRINTSiPR00794 RIBONUCLEASE
    ProDomiView protein in ProDom or Entries sharing at least one domain
    PD000535 RNaseA, 1 hit
    SMARTiView protein in SMART
    SM00092 RNAse_Pc, 1 hit
    SUPFAMiSSF54076 SSF54076, 1 hit
    PROSITEiView protein in PROSITE
    PS00127 RNASE_PANCREATIC, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRNAS6_HUMAN
    AccessioniPrimary (citable) accession number: Q93091
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 15, 1998
    Last modified: November 7, 2018
    This is version 148 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
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