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Protein

V-type proton ATPase 116 kDa subunit a isoform 1

Gene

ATP6V0A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).By similarity

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • proton-transporting ATPase activity, rotational mechanism Source: GO_Central

GO - Biological processi

Keywordsi

Biological processHydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000033627-MONOMER
ReactomeiR-HSA-1222556 ROS, RNS production in phagocytes
R-HSA-6798695 Neutrophil degranulation
R-HSA-77387 Insulin receptor recycling
R-HSA-917977 Transferrin endocytosis and recycling
R-HSA-983712 Ion channel transport

Protein family/group databases

TCDBi3.A.2.2.4 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase 116 kDa subunit a isoform 1
Short name:
V-ATPase 116 kDa isoform a1
Alternative name(s):
Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit
Vacuolar adenosine triphosphatase subunit Ac116
Vacuolar proton pump subunit 1
Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Gene namesi
Name:ATP6V0A1
Synonyms:ATP6N1, ATP6N1A, VPP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000033627.14
HGNCiHGNC:865 ATP6V0A1
MIMi192130 gene
neXtProtiNX_Q93050

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 388CytoplasmicSequence analysisAdd BLAST388
Transmembranei389 – 407HelicalSequence analysisAdd BLAST19
Topological domaini408 – 409VacuolarSequence analysis2
Transmembranei410 – 426HelicalSequence analysisAdd BLAST17
Topological domaini427 – 441CytoplasmicSequence analysisAdd BLAST15
Transmembranei442 – 471HelicalSequence analysisAdd BLAST30
Topological domaini472 – 534VacuolarSequence analysisAdd BLAST63
Transmembranei535 – 554HelicalSequence analysisAdd BLAST20
Topological domaini555 – 572CytoplasmicSequence analysisAdd BLAST18
Transmembranei573 – 593HelicalSequence analysisAdd BLAST21
Topological domaini594 – 638VacuolarSequence analysisAdd BLAST45
Transmembranei639 – 658HelicalSequence analysisAdd BLAST20
Topological domaini659 – 724CytoplasmicSequence analysisAdd BLAST66
Transmembranei725 – 749HelicalSequence analysisAdd BLAST25
Topological domaini750 – 770VacuolarSequence analysisAdd BLAST21
Transmembranei771 – 809HelicalSequence analysisAdd BLAST39
Topological domaini810 – 837CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi535
OpenTargetsiENSG00000033627
PharmGKBiPA25146

Polymorphism and mutation databases

BioMutaiATP6V0A1
DMDMi59803038

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192111 – 837V-type proton ATPase 116 kDa subunit a isoform 1Add BLAST837

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250PhosphothreonineBy similarity1
Modified residuei360PhosphothreonineCombined sources1
Modified residuei364PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ93050
PaxDbiQ93050
PeptideAtlasiQ93050
PRIDEiQ93050
ProteomicsDBi75687
75688 [Q93050-1]
75689 [Q93050-3]

PTM databases

iPTMnetiQ93050
PhosphoSitePlusiQ93050
SwissPalmiQ93050

Expressioni

Gene expression databases

BgeeiENSG00000033627 Expressed in 220 organ(s), highest expression level in frontal cortex
CleanExiHS_ATP6V0A1
ExpressionAtlasiQ93050 baseline and differential
GenevisibleiQ93050 HS

Organism-specific databases

HPAiHPA022144

Interactioni

Subunit structurei

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''. Interacts with SPAAR (PubMed:28024296).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LDHBP071952EBI-2891238,EBI-358748

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107018, 85 interactors
IntActiQ93050, 31 interactors
STRINGi9606.ENSP00000264649

Structurei

3D structure databases

ProteinModelPortaliQ93050
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2189 Eukaryota
COG1269 LUCA
GeneTreeiENSGT00390000004941
HOGENOMiHOG000037059
HOVERGENiHBG014606
InParanoidiQ93050
KOiK02154
OMAiWTAYDAH
OrthoDBiEOG091G01BI
PhylomeDBiQ93050
TreeFamiTF300346

Family and domain databases

InterProiView protein in InterPro
IPR002490 V-ATPase_116kDa_su
IPR026028 V-type_ATPase_116kDa_su_euka
PANTHERiPTHR11629 PTHR11629, 1 hit
PfamiView protein in Pfam
PF01496 V_ATPase_I, 1 hit
PIRSFiPIRSF001293 ATP6V0A1, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q93050-2) [UniParc]FASTAAdd to basket
Also known as: I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGELFRSEEM TLAQLFLQSE AAYCCVSELG ELGKVQFRDL NPDVNVFQRK
60 70 80 90 100
FVNEVRRCEE MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN
110 120 130 140 150
FEKIENELKE INTNQEALKR NFLELTELKF ILRKTQQFFD EMADPDLLEE
160 170 180 190 200
SSSLLEPSEM GRGTPLRLGF VAGVINRERI PTFERMLWRV CRGNVFLRQA
210 220 230 240 250
EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF RASLYPCPET
260 270 280 290 300
PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM
310 320 330 340 350
KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS
360 370 380 390 400
ILNRMQTNQT PPTYNKTNKF TYGFQNIVDA YGIGTYREIN PAPYTIITFP
410 420 430 440 450
FLFAVMFGDF GHGILMTLFA VWMVLRESRI LSQKNENEMF STVFSGRYII
460 470 480 490 500
LLMGVFSMYT GLIYNDCFSK SLNIFGSSWS VRPMFTYNWT EETLRGNPVL
510 520 530 540 550
QLNPALPGVF GGPYPFGIDP IWNIATNKLT FLNSFKMKMS VILGIIHMLF
560 570 580 590 600
GVSLSLFNHI YFKKPLNIYF GFIPEIIFMT SLFGYLVILI FYKWTAYDAH
610 620 630 640 650
TSENAPSLLI HFINMFLFSY PESGYSMLYS GQKGIQCFLV VVALLCVPWM
660 670 680 690 700
LLFKPLVLRR QYLRRKHLGT LNFGGIRVGN GPTEEDAEII QHDQLSTHSE
710 720 730 740 750
DADEPSEDEV FDFGDTMVHQ AIHTIEYCLG CISNTASYLR LWALSLAHAQ
760 770 780 790 800
LSEVLWTMVI HIGLSVKSLA GGLVLFFFFT AFATLTVAIL LIMEGLSAFL
810 820 830
HALRLHWVEF QNKFYSGTGF KFLPFSFEHI REGKFEE
Length:837
Mass (Da):96,413
Last modified:February 15, 2005 - v3
Checksum:i80E4842CF6ADEE44
GO
Isoform 2 (identifier: Q93050-1) [UniParc]FASTAAdd to basket
Also known as: II

The sequence of this isoform differs from the canonical sequence as follows:
     705-710: Missing.

Show »
Length:831
Mass (Da):95,756
Checksum:iB16170B568A3D586
GO
Isoform 3 (identifier: Q93050-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-141: E → EAELHHQQ
     705-710: Missing.

Note: No experimental confirmation available.
Show »
Length:838
Mass (Da):96,600
Checksum:iBE9A648B592B7C67
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EM24K7EM24_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
130Annotation score:
K7ERA0K7ERA0_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
129Annotation score:
K7ERA6K7ERA6_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
188Annotation score:
K7ENA1K7ENA1_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
191Annotation score:
B7Z641B7Z641_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
788Annotation score:
B7Z2A9B7Z2A9_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
794Annotation score:
F5H1T6F5H1T6_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
483Annotation score:
K7EPG4K7EPG4_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
198Annotation score:
K7EN36K7EN36_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
195Annotation score:
K7ELZ6K7ELZ6_HUMAN
V-type proton ATPase subunit a
ATP6V0A1
93Annotation score:
There is more potential isoformShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti750 – 751QL → HV in CAA96077 (Ref. 1) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043532141E → EAELHHQQ in isoform 3. 1 Publication1
Alternative sequenceiVSP_012814705 – 710Missing in isoform 2 and isoform 3. 3 Publications6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71460 mRNA Translation: CAA96077.1
AK295682 mRNA Translation: BAH12153.1
AK316305 mRNA Translation: BAH14676.1
AC067852 Genomic DNA No translation available.
AC107993 Genomic DNA No translation available.
CH471152 Genomic DNA Translation: EAW60830.1
BC032398 mRNA Translation: AAH32398.1
AL137683 mRNA Translation: CAB70874.1
CCDSiCCDS11426.1 [Q93050-1]
CCDS45683.1 [Q93050-3]
CCDS45684.1 [Q93050-2]
PIRiT46449
RefSeqiNP_001123492.1, NM_001130020.1 [Q93050-3]
NP_001123493.1, NM_001130021.1 [Q93050-2]
NP_005168.2, NM_005177.3 [Q93050-1]
UniGeneiHs.463074

Genome annotation databases

EnsembliENST00000264649; ENSP00000264649; ENSG00000033627 [Q93050-3]
ENST00000343619; ENSP00000342951; ENSG00000033627 [Q93050-2]
ENST00000393829; ENSP00000377415; ENSG00000033627 [Q93050-1]
GeneIDi535
KEGGihsa:535
UCSCiuc002hzq.4 human [Q93050-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71460 mRNA Translation: CAA96077.1
AK295682 mRNA Translation: BAH12153.1
AK316305 mRNA Translation: BAH14676.1
AC067852 Genomic DNA No translation available.
AC107993 Genomic DNA No translation available.
CH471152 Genomic DNA Translation: EAW60830.1
BC032398 mRNA Translation: AAH32398.1
AL137683 mRNA Translation: CAB70874.1
CCDSiCCDS11426.1 [Q93050-1]
CCDS45683.1 [Q93050-3]
CCDS45684.1 [Q93050-2]
PIRiT46449
RefSeqiNP_001123492.1, NM_001130020.1 [Q93050-3]
NP_001123493.1, NM_001130021.1 [Q93050-2]
NP_005168.2, NM_005177.3 [Q93050-1]
UniGeneiHs.463074

3D structure databases

ProteinModelPortaliQ93050
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107018, 85 interactors
IntActiQ93050, 31 interactors
STRINGi9606.ENSP00000264649

Protein family/group databases

TCDBi3.A.2.2.4 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

PTM databases

iPTMnetiQ93050
PhosphoSitePlusiQ93050
SwissPalmiQ93050

Polymorphism and mutation databases

BioMutaiATP6V0A1
DMDMi59803038

Proteomic databases

EPDiQ93050
PaxDbiQ93050
PeptideAtlasiQ93050
PRIDEiQ93050
ProteomicsDBi75687
75688 [Q93050-1]
75689 [Q93050-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264649; ENSP00000264649; ENSG00000033627 [Q93050-3]
ENST00000343619; ENSP00000342951; ENSG00000033627 [Q93050-2]
ENST00000393829; ENSP00000377415; ENSG00000033627 [Q93050-1]
GeneIDi535
KEGGihsa:535
UCSCiuc002hzq.4 human [Q93050-2]

Organism-specific databases

CTDi535
DisGeNETi535
EuPathDBiHostDB:ENSG00000033627.14
GeneCardsiATP6V0A1
H-InvDBiHIX0013842
HGNCiHGNC:865 ATP6V0A1
HPAiHPA022144
MIMi192130 gene
neXtProtiNX_Q93050
OpenTargetsiENSG00000033627
PharmGKBiPA25146
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2189 Eukaryota
COG1269 LUCA
GeneTreeiENSGT00390000004941
HOGENOMiHOG000037059
HOVERGENiHBG014606
InParanoidiQ93050
KOiK02154
OMAiWTAYDAH
OrthoDBiEOG091G01BI
PhylomeDBiQ93050
TreeFamiTF300346

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000033627-MONOMER
ReactomeiR-HSA-1222556 ROS, RNS production in phagocytes
R-HSA-6798695 Neutrophil degranulation
R-HSA-77387 Insulin receptor recycling
R-HSA-917977 Transferrin endocytosis and recycling
R-HSA-983712 Ion channel transport

Miscellaneous databases

ChiTaRSiATP6V0A1 human
GeneWikiiATPase,_H%2B_transporting,_lysosomal_V0_subunit_a1
GenomeRNAii535
PROiPR:Q93050
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000033627 Expressed in 220 organ(s), highest expression level in frontal cortex
CleanExiHS_ATP6V0A1
ExpressionAtlasiQ93050 baseline and differential
GenevisibleiQ93050 HS

Family and domain databases

InterProiView protein in InterPro
IPR002490 V-ATPase_116kDa_su
IPR026028 V-type_ATPase_116kDa_su_euka
PANTHERiPTHR11629 PTHR11629, 1 hit
PfamiView protein in Pfam
PF01496 V_ATPase_I, 1 hit
PIRSFiPIRSF001293 ATP6V0A1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVPP1_HUMAN
AccessioniPrimary (citable) accession number: Q93050
Secondary accession number(s): B7Z3B7, Q8N5G7, Q9NSX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 15, 2005
Last modified: September 12, 2018
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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