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UniProtKB - Q92PB6 (FUMC_RHIME)
Protein
Fumarate hydratase class II
Gene
fumC
Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Functioni
Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.
UniRule annotationMiscellaneous
There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation
Catalytic activityi
- EC:4.2.1.2UniRule annotation
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 187 | SubstrateUniRule annotation | 1 | |
Active sitei | 188 | Proton donor/acceptorUniRule annotation | 1 | |
Active sitei | 318 | UniRule annotation | 1 | |
Binding sitei | 319 | SubstrateUniRule annotation | 1 | |
Sitei | 331 | Important for catalytic activityUniRule annotation | 1 |
GO - Molecular functioni
- fumarate hydratase activity Source: UniProtKB-UniRule
GO - Biological processi
- fumarate metabolic process Source: InterPro
- tricarboxylic acid cycle Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Tricarboxylic acid cycle |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER01007 |
Names & Taxonomyi
Protein namesi | Recommended name: Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)Short name: Fumarase CUniRule annotation Alternative name(s): Aerobic fumaraseUniRule annotation Iron-independent fumaraseUniRule annotation |
Gene namesi | Name:fumCUniRule annotation Ordered Locus Names:R01859 ORF Names:SMc00149 |
Organismi | Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) |
Taxonomic identifieri | 266834 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Hyphomicrobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- tricarboxylic acid cycle enzyme complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161305 | 1 – 463 | Fumarate hydratase class IIAdd BLAST | 463 |
Interactioni
Subunit structurei
Homotetramer.
UniRule annotation1 PublicationProtein-protein interaction databases
STRINGi | 266834.SMc00149 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q92PB6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 98 – 100 | Substrate bindingUniRule annotation | 3 | |
Regioni | 129 – 132 | Substrate binding (B site)UniRule annotation | 4 | |
Regioni | 139 – 141 | Substrate bindingUniRule annotation | 3 | |
Regioni | 324 – 326 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0114, Bacteria |
Family and domain databases
CDDi | cd01362, Fumarase_classII, 1 hit |
Gene3Di | 1.10.275.10, 1 hit |
HAMAPi | MF_00743, FumaraseC, 1 hit |
InterProi | View protein in InterPro IPR005677, Fum_hydII IPR024083, Fumarase/histidase_N IPR018951, Fumarase_C_C IPR020557, Fumarate_lyase_CS IPR000362, Fumarate_lyase_fam IPR022761, Fumarate_lyase_N IPR008948, L-Aspartase-like |
PANTHERi | PTHR11444, PTHR11444, 1 hit |
Pfami | View protein in Pfam PF10415, FumaraseC_C, 1 hit PF00206, Lyase_1, 1 hit |
PRINTSi | PR00149, FUMRATELYASE |
SUPFAMi | SSF48557, SSF48557, 1 hit |
TIGRFAMsi | TIGR00979, fumC_II, 1 hit |
PROSITEi | View protein in PROSITE PS00163, FUMARATE_LYASES, 1 hit |
i Sequence
Sequence statusi: Complete.
Q92PB6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSTRTETDT FGPIEVASDR YWGAQAQRSL GNFKIGWEKQ PLAIVRALGI
60 70 80 90 100
VKQAAARANM ALGRLDPAIG DAIVKAAQEV IDGKLDEHFP LVVWQTGSGT
110 120 130 140 150
QSNMNANEVV SNRAIELLGG VMGSKKPVHP NDHVNMSQSS NDTYPTAMHI
160 170 180 190 200
ACAERVIHDL LPALKHLHKA LEEKVKAFDH IIKIGRTHTQ DATPLTLGQE
210 220 230 240 250
FSGYAAQVAS SIKRIEMTLP GLCELAQGGT AVGTGLNAPV GFAEKVAEEI
260 270 280 290 300
AAITGIGFTS APNKFEALAA HDSMVFSHGA INATAAALFK IANDIRFLGS
310 320 330 340 350
GPRSGLGELS LPENEPGSSI MPGKVNPTQC EALTQVCVQV FGNHAALTFA
360 370 380 390 400
GSQGHFELNV YNPLMAYNFL QSVQLLADAA ISFTDNCVVG IEAREDNIKA
410 420 430 440 450
ALDRSLMLVT ALAPKIGYDN AAKIAKTAHK NGTTLREEAV GGGYVTDEEF
460
DAVVRPETMI GPA
Sequence cautioni
The sequence CAC46438 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL591688 Genomic DNA Translation: CAC46438.1 Different initiation. |
RefSeqi | NP_385965.3, NC_003047.1 |
Genome annotation databases
EnsemblBacteriai | CAC46438; CAC46438; SMc00149 |
KEGGi | sme:SMc00149 |
PATRICi | fig|266834.11.peg.3302 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL591688 Genomic DNA Translation: CAC46438.1 Different initiation. |
RefSeqi | NP_385965.3, NC_003047.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4HGV | X-ray | 2.09 | A/B/C/D | 1-463 | [»] | |
SMRi | Q92PB6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 266834.SMc00149 |
Genome annotation databases
EnsemblBacteriai | CAC46438; CAC46438; SMc00149 |
KEGGi | sme:SMc00149 |
PATRICi | fig|266834.11.peg.3302 |
Phylogenomic databases
eggNOGi | COG0114, Bacteria |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER01007 |
Family and domain databases
CDDi | cd01362, Fumarase_classII, 1 hit |
Gene3Di | 1.10.275.10, 1 hit |
HAMAPi | MF_00743, FumaraseC, 1 hit |
InterProi | View protein in InterPro IPR005677, Fum_hydII IPR024083, Fumarase/histidase_N IPR018951, Fumarase_C_C IPR020557, Fumarate_lyase_CS IPR000362, Fumarate_lyase_fam IPR022761, Fumarate_lyase_N IPR008948, L-Aspartase-like |
PANTHERi | PTHR11444, PTHR11444, 1 hit |
Pfami | View protein in Pfam PF10415, FumaraseC_C, 1 hit PF00206, Lyase_1, 1 hit |
PRINTSi | PR00149, FUMRATELYASE |
SUPFAMi | SSF48557, SSF48557, 1 hit |
TIGRFAMsi | TIGR00979, fumC_II, 1 hit |
PROSITEi | View protein in PROSITE PS00163, FUMARATE_LYASES, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FUMC_RHIME | |
Accessioni | Q92PB6Primary (citable) accession number: Q92PB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 2003 |
Last sequence update: | December 15, 2003 | |
Last modified: | September 29, 2021 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families