We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q92ET9 (DHAM2_LISIN)
Protein
PEP-dependent dihydroxyacetone kinase 2, phosphoryl donor subunit DhaM
Gene
dhaM-2
Organism
Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)
Status
Functioni
Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.
1 PublicationMiscellaneous
Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKML has no transport activity.1 Publication
Catalytic activityi
- EC:2.7.1.1211 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 9 | Tele-phosphohistidine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- phosphoenolpyruvate-glycerone phosphotransferase activity Source: UniProtKB
GO - Biological processi
- glycerol catabolic process Source: InterPro
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Glycerol metabolism, Phosphotransferase system |
Names & Taxonomyi
Protein namesi | Recommended name: PEP-dependent dihydroxyacetone kinase 2, phosphoryl donor subunit DhaM1 Publication (EC:2.7.1.1211 Publication)Alternative name(s): PTS system EIIA component1 Publication Phosphotransferase enzyme IIA component1 Publication |
Gene namesi | Name:dhaM-21 Publication Ordered Locus Names:lin0369Imported |
Organismi | Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262) |
Taxonomic identifieri | 272626 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000439403 | 1 – 125 | PEP-dependent dihydroxyacetone kinase 2, phosphoryl donor subunit DhaMAdd BLAST | 125 |
Expressioni
Inductioni
Repressed by GolR.1 Publication
Interactioni
Subunit structurei
Homodimer. The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.
By similarityProtein-protein interaction databases
STRINGi | 272626.lin0369 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 125 | PTS EIIA type-4PROSITE-ProRule annotationAdd BLAST | 125 |
Domaini
The EIIA type-4 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB type-4 domain.PROSITE-ProRule annotation
Sequence similaritiesi
Belongs to the PEP-utilizing enzyme family.Curated
Phylogenomic databases
eggNOGi | COG3412, Bacteria |
HOGENOMi | CLU_045361_2_2_9 |
OMAi | DMVRQMV |
OrthoDBi | 2041582at2 |
Family and domain databases
Gene3Di | 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit |
SUPFAMi | SSF53062, SSF53062, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit |
i Sequence
Sequence statusi: Complete.
Q92ET9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MISIVLVSHS QKITEGLQEM IVEMVGDTVH IISSGGTGDG RLGTNALMIA
60 70 80 90 100
DNIATCTNSE HIYIFCDIGS AILSAETALE LLDTELLEKT TIIDAPLVEG
110 120
AFTAAVQSLV NPSKEAILQE LTNVH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL596164 Genomic DNA Translation: CAC95602.1 |
PIRi | AB1479 |
RefSeqi | WP_003770298.1, NC_003212.1 |
Genome annotation databases
EnsemblBacteriai | CAC95602; CAC95602; CAC95602 |
KEGGi | lin:lin0369 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL596164 Genomic DNA Translation: CAC95602.1 |
PIRi | AB1479 |
RefSeqi | WP_003770298.1, NC_003212.1 |
3D structure databases
SMRi | Q92ET9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 272626.lin0369 |
Genome annotation databases
EnsemblBacteriai | CAC95602; CAC95602; CAC95602 |
KEGGi | lin:lin0369 |
Phylogenomic databases
eggNOGi | COG3412, Bacteria |
HOGENOMi | CLU_045361_2_2_9 |
OMAi | DMVRQMV |
OrthoDBi | 2041582at2 |
Family and domain databases
Gene3Di | 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit |
SUPFAMi | SSF53062, SSF53062, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHAM2_LISIN | |
Accessioni | Q92ET9Primary (citable) accession number: Q92ET9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2017 |
Last sequence update: | December 1, 2001 | |
Last modified: | June 2, 2021 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |