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UniProtKB - Q92973 (TNPO1_HUMAN)

Protein

Transportin-1

Gene

TNPO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:24753571). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19 (By similarity). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed:19124606, PubMed:24753571).By similarity5 Publications
(Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei468Important for interaction with cargo nuclear localization signals1 Publication1
Sitei738Important for interaction with cargo nuclear localization signals1 Publication1

GO - Molecular functioni

  • nuclear localization sequence binding Source: ProtInc
  • protein transporter activity Source: GO_Central
  • Ran GTPase binding Source: InterPro
  • RNA binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processHost-virus interaction, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-450513 Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA
R-HSA-5620924 Intraflagellar transport

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Transportin-1
Alternative name(s):
Importin beta-2
Karyopherin beta-2
M9 region interaction protein
Short name:
MIP
Gene namesi
Name:TNPO1
Synonyms:KPNB2, MIP1, TRN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000083312.17
HGNCiHGNC:6401 TNPO1
MIMi602901 gene
neXtProtiNX_Q92973

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi468W → A: Abolishes interaction with the ADAR nuclear localization signal. Abolishes ADAR nuclear import. 1 Publication1
Mutagenesisi738W → A: Abolishes interaction with the ADAR nuclear localization signal. Abolishes ADAR nuclear import. 1 Publication1

Organism-specific databases

DisGeNETi3842
OpenTargetsiENSG00000083312
PharmGKBiPA30192

Polymorphism and mutation databases

BioMutaiTNPO1
DMDMi259016171

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207651 – 898Transportin-1Add BLAST898

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q92973-2)
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ92973
MaxQBiQ92973
PaxDbiQ92973
PeptideAtlasiQ92973
PRIDEiQ92973
ProteomicsDBi75635
75636 [Q92973-2]
75637 [Q92973-3]

PTM databases

iPTMnetiQ92973
PhosphoSitePlusiQ92973
SwissPalmiQ92973

Expressioni

Gene expression databases

BgeeiENSG00000083312 Expressed in 249 organ(s), highest expression level in thoracic mammary gland
CleanExiHS_TNPO1
ExpressionAtlasiQ92973 baseline and differential
GenevisibleiQ92973 HS

Organism-specific databases

HPAiCAB016325

Interactioni

Subunit structurei

Identified in a complex that contains TNPO1, RAN and RANBP1 (PubMed:9428644). Binds HNRPA1, HNRPA2, HNRNPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Interacts with isoform 1 and isoform 5 of ADAR/ADAR1 (via DRBM 3 domain) (PubMed:19124606, PubMed:24753571). Interacts with SNAI1 (via zinc fingers); the interaction mediates SNAI1 nuclear import (PubMed:19386897). Interacts with SNAI2 (via zinc fingers) (PubMed:19386897).By similarity6 Publications
(Microbial infection) Binds to HIV-1 Rev.1 Publication

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi110040, 85 interactors
DIPiDIP-29335N
IntActiQ92973, 46 interactors
MINTiQ92973
STRINGi9606.ENSP00000336712

Structurei

Secondary structure

1898
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92973
SMRiQ92973
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92973

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati19 – 46HEAT 11 PublicationAdd BLAST28
Domaini41 – 109Importin N-terminalPROSITE-ProRule annotationAdd BLAST69
Repeati51 – 89HEAT 21 PublicationAdd BLAST39
Repeati98 – 131HEAT 31 PublicationAdd BLAST34
Repeati137 – 174HEAT 41 PublicationAdd BLAST38
Repeati181 – 211HEAT 51 PublicationAdd BLAST31
Repeati224 – 251HEAT 61 PublicationAdd BLAST28
Repeati263 – 290HEAT 71 PublicationAdd BLAST28
Repeati306 – 397HEAT 81 PublicationAdd BLAST92
Repeati405 – 433HEAT 91 PublicationAdd BLAST29
Repeati445 – 472HEAT 101 PublicationAdd BLAST28
Repeati486 – 519HEAT 111 PublicationAdd BLAST34
Repeati527 – 560HEAT 121 PublicationAdd BLAST34
Repeati568 – 606HEAT 131 PublicationAdd BLAST39
Repeati614 – 665HEAT 141 PublicationAdd BLAST52
Repeati676 – 707HEAT 151 PublicationAdd BLAST32
Repeati715 – 748HEAT 161 PublicationAdd BLAST34
Repeati756 – 791HEAT 171 PublicationAdd BLAST36
Repeati799 – 832HEAT 181 PublicationAdd BLAST34
Repeati841 – 872HEAT 191 PublicationAdd BLAST32
Repeati875 – 895HEAT 201 PublicationAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi358 – 376Asp/Glu-rich (acidic)Add BLAST19

Sequence similaritiesi

Belongs to the importin beta family. Importin beta-2 subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2023 Eukaryota
ENOG410XPK2 LUCA
GeneTreeiENSGT00550000074720
HOGENOMiHOG000203940
HOVERGENiHBG058963
InParanoidiQ92973
KOiK18752
OMAiHNFPNGV
OrthoDBiEOG091G01O2
PhylomeDBiQ92973
TreeFamiTF300825

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50166 IMPORTIN_B_NT, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q92973-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVWDRQTKME YEWKPDEQGL QQILQLLKES QSPDTTIQRT VQQKLEQLNQ 
        60         70         80         90        100
YPDFNNYLIF VLTKLKSEDE PTRSLSGLIL KNNVKAHFQN FPNGVTDFIK 
       110        120        130        140        150
SECLNNIGDS SPLIRATVGI LITTIASKGE LQNWPDLLPK LCSLLDSEDY 
       160        170        180        190        200
NTCEGAFGAL QKICEDSAEI LDSDVLDRPL NIMIPKFLQF FKHSSPKIRS 
       210        220        230        240        250
HAVACVNQFI ISRTQALMLH IDSFIENLFA LAGDEEPEVR KNVCRALVML 
       260        270        280        290        300
LEVRMDRLLP HMHNIVEYML QRTQDQDENV ALEACEFWLT LAEQPICKDV 
       310        320        330        340        350
LVRHLPKLIP VLVNGMKYSD IDIILLKGDV EEDETIPDSE QDIRPRFHRS 
       360        370        380        390        400
RTVAQQHDED GIEEEDDDDD EIDDDDTISD WNLRKCSAAA LDVLANVYRD 
       410        420        430        440        450
ELLPHILPLL KELLFHHEWV VKESGILVLG AIAEGCMQGM IPYLPELIPH 
       460        470        480        490        500
LIQCLSDKKA LVRSITCWTL SRYAHWVVSQ PPDTYLKPLM TELLKRILDS 
       510        520        530        540        550
NKRVQEAACS AFATLEEEAC TELVPYLAYI LDTLVFAFSK YQHKNLLILY 
       560        570        580        590        600
DAIGTLADSV GHHLNKPEYI QMLMPPLIQK WNMLKDEDKD LFPLLECLSS 
       610        620        630        640        650
VATALQSGFL PYCEPVYQRC VNLVQKTLAQ AMLNNAQPDQ YEAPDKDFMI 
       660        670        680        690        700
VALDLLSGLA EGLGGNIEQL VARSNILTLM YQCMQDKMPE VRQSSFALLG 
       710        720        730        740        750
DLTKACFQHV KPCIADFMPI LGTNLNPEFI SVCNNATWAI GEISIQMGIE 
       760        770        780        790        800
MQPYIPMVLH QLVEIINRPN TPKTLLENTA ITIGRLGYVC PQEVAPMLQQ 
       810        820        830        840        850
FIRPWCTSLR NIRDNEEKDS AFRGICTMIS VNPSGVIQDF IFFCDAVASW 
       860        870        880        890 
INPKDDLRDM FCKILHGFKN QVGDENWRRF SDQFPLPLKE RLAAFYGV
Length:898
Mass (Da):102,355
Last modified:September 22, 2009 - v2
Checksum:i7B880D9E7CA6798F
GO
Isoform 2 (identifier: Q92973-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MVWDRQTKM → M

Show »
Length:890
Mass (Da):101,310
Checksum:i4762DB4895472D10
GO
Isoform 3 (identifier: Q92973-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-118: Missing.

Show »
Length:848
Mass (Da):96,901
Checksum:i6DF034D06DC572B4
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EW37E7EW37_HUMAN
Transportin-1
TNPO1
136Annotation score:
S4R398S4R398_HUMAN
Transportin-1
TNPO1
193Annotation score:

Sequence cautioni

The sequence AAC50723 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH40340 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104L → S in AAB68948 (Ref. 3) Curated1
Sequence conflicti225I → T in AAC50723 (PubMed:8808633).Curated1
Sequence conflicti669Q → L in AAB68948 (Ref. 3) Curated1
Sequence conflicti880F → L in BAG62638 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0380281 – 9MVWDRQTKM → M in isoform 2. 2 Publications9
Alternative sequenceiVSP_03802969 – 118Missing in isoform 3. 1 PublicationAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70322 mRNA Translation: AAC50723.1 Different initiation.
U72069 mRNA Translation: AAB58254.1
U72395 mRNA Translation: AAB68948.1
AK301021 mRNA Translation: BAG62638.1
BC040340 mRNA Translation: AAH40340.1 Different initiation.
CCDSiCCDS4016.1 [Q92973-2]
CCDS43329.1 [Q92973-1]
RefSeqiNP_002261.3, NM_002270.3 [Q92973-1]
NP_694858.1, NM_153188.2 [Q92973-2]
XP_005248557.1, XM_005248500.2 [Q92973-2]
UniGeneiHs.482497

Genome annotation databases

EnsembliENST00000337273; ENSP00000336712; ENSG00000083312 [Q92973-1]
ENST00000506351; ENSP00000425118; ENSG00000083312 [Q92973-2]
ENST00000523768; ENSP00000428899; ENSG00000083312 [Q92973-3]
GeneIDi3842
KEGGihsa:3842
UCSCiuc003kci.5 human [Q92973-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70322 mRNA Translation: AAC50723.1 Different initiation.
U72069 mRNA Translation: AAB58254.1
U72395 mRNA Translation: AAB68948.1
AK301021 mRNA Translation: BAG62638.1
BC040340 mRNA Translation: AAH40340.1 Different initiation.
CCDSiCCDS4016.1 [Q92973-2]
CCDS43329.1 [Q92973-1]
RefSeqiNP_002261.3, NM_002270.3 [Q92973-1]
NP_694858.1, NM_153188.2 [Q92973-2]
XP_005248557.1, XM_005248500.2 [Q92973-2]
UniGeneiHs.482497

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QBKX-ray3.00B9-898[»]
2H4MX-ray3.05A/B376-898[»]
A/B9-343[»]
2OT8X-ray3.10A/B9-331[»]
A/B375-898[»]
2QMRX-ray3.00A/B/C/D9-898[»]
2Z5JX-ray3.40A9-898[»]
2Z5KX-ray2.60A9-898[»]
2Z5MX-ray3.00A9-898[»]
2Z5NX-ray3.20A9-898[»]
2Z5OX-ray3.20A9-898[»]
4FDDX-ray2.30A9-331[»]
A375-898[»]
4FQ3X-ray3.00A9-898[»]
4JLQX-ray3.05A9-331[»]
A375-898[»]
4OO6X-ray2.70A375-898[»]
A9-331[»]
5J3VX-ray3.05A/B9-331[»]
A/B375-898[»]
5TQCX-ray3.00A9-343[»]
A375-898[»]
5YVGX-ray4.05A/B9-344[»]
A/B376-898[»]
5YVHX-ray3.15A9-344[»]
A376-898[»]
5YVIX-ray2.90A9-344[»]
A376-898[»]
ProteinModelPortaliQ92973
SMRiQ92973
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110040, 85 interactors
DIPiDIP-29335N
IntActiQ92973, 46 interactors
MINTiQ92973
STRINGi9606.ENSP00000336712

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiQ92973
PhosphoSitePlusiQ92973
SwissPalmiQ92973

Polymorphism and mutation databases

BioMutaiTNPO1
DMDMi259016171

Proteomic databases

EPDiQ92973
MaxQBiQ92973
PaxDbiQ92973
PeptideAtlasiQ92973
PRIDEiQ92973
ProteomicsDBi75635
75636 [Q92973-2]
75637 [Q92973-3]

Protocols and materials databases

DNASUi3842
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337273; ENSP00000336712; ENSG00000083312 [Q92973-1]
ENST00000506351; ENSP00000425118; ENSG00000083312 [Q92973-2]
ENST00000523768; ENSP00000428899; ENSG00000083312 [Q92973-3]
GeneIDi3842
KEGGihsa:3842
UCSCiuc003kci.5 human [Q92973-1]

Organism-specific databases

CTDi3842
DisGeNETi3842
EuPathDBiHostDB:ENSG00000083312.17
GeneCardsiTNPO1
HGNCiHGNC:6401 TNPO1
HPAiCAB016325
MIMi602901 gene
neXtProtiNX_Q92973
OpenTargetsiENSG00000083312
PharmGKBiPA30192
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2023 Eukaryota
ENOG410XPK2 LUCA
GeneTreeiENSGT00550000074720
HOGENOMiHOG000203940
HOVERGENiHBG058963
InParanoidiQ92973
KOiK18752
OMAiHNFPNGV
OrthoDBiEOG091G01O2
PhylomeDBiQ92973
TreeFamiTF300825

Enzyme and pathway databases

ReactomeiR-HSA-450513 Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA
R-HSA-5620924 Intraflagellar transport

Miscellaneous databases

ChiTaRSiTNPO1 human
EvolutionaryTraceiQ92973
GeneWikiiTransportin_1
GenomeRNAii3842
PROiPR:Q92973
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000083312 Expressed in 249 organ(s), highest expression level in thoracic mammary gland
CleanExiHS_TNPO1
ExpressionAtlasiQ92973 baseline and differential
GenevisibleiQ92973 HS

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50166 IMPORTIN_B_NT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTNPO1_HUMAN
AccessioniPrimary (citable) accession number: Q92973
Secondary accession number(s): B4DVC6, Q92957, Q92975
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 22, 2009
Last modified: October 10, 2018
This is version 197 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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