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Protein

Potassium voltage-gated channel subfamily B member 2

Gene

KCNB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and smooth muscle cells. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient. Homotetrameric channels mediate a delayed-rectifier voltage-dependent outward potassium current that display rapid activation and slow inactivation in response to membrane depolarization. Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNB1; channel properties depend on the type of alpha subunits that are part of the channel. Can also form functional heterotetrameric channels with other alpha subunits that are non-conducting when expressed alone, such as KCNS1 and KCNS2, creating a functionally diverse range of channel complexes. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Contributes to the delayed-rectifier voltage-gated potassium current in cortical pyramidal neurons and smooth muscle cells.By similarity

Activity regulationi

Inhibited by quinine at micromolar levels. Modestly sensitive to millimolar levels of tetraethylammonium (TEA) and 4-aminopyridine (4-AP).By similarity1 Publication

Kineticsi

Homotetrameric channels expressed in xenopus oocytes or in mammalian non-neuronal cells display delayed-rectifier voltage-dependent potassium currents which are activated during membrane depolarization, i.e within a risetime of about 20 msec. After that, inactivate very slowly. Their activation requires low threshold potentials of about -20 to -30 mV, with a midpoint activation at about 10 mV. For inactivation, the voltage at half-maximal amplitude is about -30 mV. Channels have an unitary conductance of about 14 pS. The voltage-dependence of activation and inactivation and other channel characteristics vary depending on the experimental conditions, the expression system and post-translational modifications.1 Publication

      GO - Molecular functioni

      GO - Biological processi

      Keywordsi

      Molecular functionIon channel, Potassium channel, Voltage-gated channel
      Biological processIon transport, Potassium transport, Transport
      LigandPotassium

      Enzyme and pathway databases

      ReactomeiR-HSA-1296072 Voltage gated Potassium channels

      Protein family/group databases

      TCDBi1.A.1.2.25 the voltage-gated ion channel (vic) superfamily

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Potassium voltage-gated channel subfamily B member 2Imported
      Alternative name(s):
      Voltage-gated potassium channel subunit Kv2.2
      Gene namesi
      Name:KCNB2Imported
      OrganismiHomo sapiens (Human)
      Taxonomic identifieri9606 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
      Proteomesi
      • UP000005640 Componenti: Chromosome 8

      Organism-specific databases

      EuPathDBiHostDB:ENSG00000182674.5
      HGNCiHGNC:6232 KCNB2
      MIMi607738 gene
      neXtProtiNX_Q92953

      Subcellular locationi

      Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

      Topology

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Topological domaini1 – 190CytoplasmicBy similarityAdd BLAST190
      Transmembranei191 – 212Helical; Name=Segment S1By similarityAdd BLAST22
      Topological domaini213 – 232ExtracellularBy similarityAdd BLAST20
      Transmembranei233 – 254Helical; Name=Segment S2By similarityAdd BLAST22
      Topological domaini255 – 265CytoplasmicBy similarityAdd BLAST11
      Transmembranei266 – 284Helical; Name=Segment S3By similarityAdd BLAST19
      Topological domaini285 – 296ExtracellularBy similarityAdd BLAST12
      Transmembranei297 – 317Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST21
      Topological domaini318 – 332CytoplasmicBy similarityAdd BLAST15
      Transmembranei333 – 354Helical; Name=Segment S5By similarityAdd BLAST22
      Topological domaini355 – 368ExtracellularBy similarityAdd BLAST14
      Intramembranei369 – 380Helical; Name=Pore helixBy similarityAdd BLAST12
      Intramembranei381 – 388By similarity8
      Topological domaini389 – 395ExtracellularBy similarity7
      Transmembranei396 – 424Helical; Name=Segment S6By similarityAdd BLAST29
      Topological domaini425 – 911CytoplasmicBy similarityAdd BLAST487

      Keywords - Cellular componenti

      Cell membrane, Cell projection, Membrane

      Pathology & Biotechi

      Organism-specific databases

      DisGeNETi9312
      OpenTargetsiENSG00000182674
      PharmGKBiPA30025

      Chemistry databases

      ChEMBLiCHEMBL2321618
      DrugBankiDB06637 Dalfampridine
      GuidetoPHARMACOLOGYi547

      Polymorphism and mutation databases

      BioMutaiKCNB2
      DMDMi24418855

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      ChainiPRO_00000540481 – 911Potassium voltage-gated channel subfamily B member 2Add BLAST911

      Amino acid modifications

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
      Modified residuei448PhosphoserineBy similarity1

      Post-translational modificationi

      Phosphorylated.By similarity

      Keywords - PTMi

      Glycoprotein, Phosphoprotein

      Proteomic databases

      EPDiQ92953
      PaxDbiQ92953
      PeptideAtlasiQ92953
      PRIDEiQ92953
      ProteomicsDBi75623

      PTM databases

      iPTMnetiQ92953
      PhosphoSitePlusiQ92953

      Expressioni

      Gene expression databases

      BgeeiENSG00000182674 Expressed in 69 organ(s), highest expression level in buccal mucosa cell
      CleanExiHS_KCNB2
      GenevisibleiQ92953 HS

      Organism-specific databases

      HPAiHPA065970

      Interactioni

      Subunit structurei

      Homotetramer or heterotetramer with KCNB1. Heterotetramer with KCNS1 and KCNS2.By similarity

      GO - Molecular functioni

      Protein-protein interaction databases

      BioGridi114724, 7 interactors
      IntActiQ92953, 5 interactors
      MINTiQ92953
      STRINGi9606.ENSP00000430846

      Chemistry databases

      BindingDBiQ92953

      Structurei

      3D structure databases

      ProteinModelPortaliQ92953
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Motif

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Motifi381 – 386Selectivity filterBy similarity6

      Domaini

      The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

      Sequence similaritiesi

      Keywords - Domaini

      Transmembrane, Transmembrane helix

      Phylogenomic databases

      eggNOGiKOG3713 Eukaryota
      COG1226 LUCA
      GeneTreeiENSGT00760000118981
      HOGENOMiHOG000113206
      HOVERGENiHBG052225
      InParanoidiQ92953
      KOiK04886
      OMAiKMENHLF
      OrthoDBiEOG091G0FP3
      PhylomeDBiQ92953
      TreeFamiTF313103

      Family and domain databases

      Gene3Di1.20.120.350, 1 hit
      InterProiView protein in InterPro
      IPR000210 BTB/POZ_dom
      IPR005821 Ion_trans_dom
      IPR003968 K_chnl_volt-dep_Kv
      IPR003973 K_chnl_volt-dep_Kv2
      IPR005826 K_chnl_volt-dep_Kv2.2
      IPR011333 SKP1/BTB/POZ_sf
      IPR003131 T1-type_BTB
      IPR028325 VG_K_chnl
      IPR027359 Volt_channel_dom_sf
      PANTHERiPTHR11537 PTHR11537, 1 hit
      PfamiView protein in Pfam
      PF02214 BTB_2, 1 hit
      PF00520 Ion_trans, 1 hit
      PF03521 Kv2channel, 1 hit
      PRINTSiPR00169 KCHANNEL
      PR01515 KV22CHANNEL
      PR01491 KVCHANNEL
      PR01495 SHABCHANNEL
      SMARTiView protein in SMART
      SM00225 BTB, 1 hit
      SUPFAMiSSF54695 SSF54695, 1 hit

      Sequencei

      Sequence statusi: Complete.

      Q92953-1 [UniParc]FASTAAdd to basket
      « Hide
              10         20         30         40         50
      MAEKAPPGLN RKTSRSTLSL PPEPVDIIRS KTCSRRVKIN VGGLNHEVLW
      60 70 80 90 100
      RTLDRLPRTR LGKLRDCNTH ESLLEVCDDY NLNENEYFFD RHPGAFTSIL
      110 120 130 140 150
      NFYRTGKLHM MEEMCALSFG QELDYWGIDE IYLESCCQAR YHQKKEQMNE
      160 170 180 190 200
      ELRREAETMR EREGEEFDNT CCPDKRKKLW DLLEKPNSSV AAKILAIVSI
      210 220 230 240 250
      LFIVLSTIAL SLNTLPELQE TDEFGQLNDN RQLAHVEAVC IAWFTMEYLL
      260 270 280 290 300
      RFLSSPNKWK FFKGPLNVID LLAILPYYVT IFLTESNKSV LQFQNVRRVV
      310 320 330 340 350
      QIFRIMRILR ILKLARHSTG LQSLGFTLRR SYNELGLLIL FLAMGIMIFS
      360 370 380 390 400
      SLVFFAEKDE DATKFTSIPA SFWWATITMT TVGYGDIYPK TLLGKIVGGL
      410 420 430 440 450
      CCIAGVLVIA LPIPIIVNNF SEFYKEQKRQ EKAIKRREAL ERAKRNGSIV
      460 470 480 490 500
      SMNLKDAFAR SMELIDVAVE KAGESANTKD SADDNHLSPS RWKWARKALS
      510 520 530 540 550
      ETSSNKSFEN KYQEVSQKDS HEQLNNTSSS SPQHLSAQKL EMLYNEITKT
      560 570 580 590 600
      QPHSHPNPDC QEKPERPSAY EEEIEMEEVV CPQEQLAVAQ TEVIVDMKST
      610 620 630 640 650
      SSIDSFTSCA TDFTETERSP LPPPSASHLQ MKFPTDLPGT EEHQRARGPP
      660 670 680 690 700
      FLTLSREKGP AARDGTLEYA PVDITVNLDA SGSQCGLHSP LQSDNATDSP
      710 720 730 740 750
      KSSLKGSNPL KSRSLKVNFK ENRGSAPQTP PSTARPLPVT TADFSLTTPQ
      760 770 780 790 800
      HISTILLEET PSQGDRPLLG TEVSAPCQGP SKGLSPRFPK QKLFPFSSRE
      810 820 830 840 850
      RRSFTEIDTG DDEDFLELPG AREEKQVDSS PNCFADKPSD GRDPLREEGS
      860 870 880 890 900
      VGSSSPQDTG HNCRQDIYHA VSEVKKDSSQ EGCKMENHLF APEIHSNPGD
      910
      TGYCPTRETS M
      Length:911
      Mass (Da):102,563
      Last modified:October 25, 2002 - v2
      Checksum:i2261D7D6280CF81A
      GO

      Sequence cautioni

      The sequence AAB08433 differs from that shown. Reason: Frameshift at position 768.Curated

      Experimental Info

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Sequence conflicti161 – 162ER → DG in AAP46292 (Ref. 1) Curated2
      Sequence conflicti528S → F in AAP46292 (Ref. 1) Curated1

      Natural variant

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Natural variantiVAR_035774450V → I in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs770305852Ensembl.1
      Natural variantiVAR_034050657E → G. Corresponds to variant dbSNP:rs16938507Ensembl.1

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AF450111 mRNA Translation: AAP46292.1
      AF338730 mRNA Translation: AAK16585.1
      U69962 mRNA Translation: AAB08433.1 Frameshift.
      CCDSiCCDS6209.1
      RefSeqiNP_004761.2, NM_004770.2
      UniGeneiHs.661102

      Genome annotation databases

      EnsembliENST00000523207; ENSP00000430846; ENSG00000182674
      GeneIDi9312
      KEGGihsa:9312
      UCSCiuc003xzb.3 human

      Keywords - Coding sequence diversityi

      Polymorphism

      Similar proteinsi

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AF450111 mRNA Translation: AAP46292.1
      AF338730 mRNA Translation: AAK16585.1
      U69962 mRNA Translation: AAB08433.1 Frameshift.
      CCDSiCCDS6209.1
      RefSeqiNP_004761.2, NM_004770.2
      UniGeneiHs.661102

      3D structure databases

      ProteinModelPortaliQ92953
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      BioGridi114724, 7 interactors
      IntActiQ92953, 5 interactors
      MINTiQ92953
      STRINGi9606.ENSP00000430846

      Chemistry databases

      BindingDBiQ92953
      ChEMBLiCHEMBL2321618
      DrugBankiDB06637 Dalfampridine
      GuidetoPHARMACOLOGYi547

      Protein family/group databases

      TCDBi1.A.1.2.25 the voltage-gated ion channel (vic) superfamily

      PTM databases

      iPTMnetiQ92953
      PhosphoSitePlusiQ92953

      Polymorphism and mutation databases

      BioMutaiKCNB2
      DMDMi24418855

      Proteomic databases

      EPDiQ92953
      PaxDbiQ92953
      PeptideAtlasiQ92953
      PRIDEiQ92953
      ProteomicsDBi75623

      Protocols and materials databases

      DNASUi9312
      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      EnsembliENST00000523207; ENSP00000430846; ENSG00000182674
      GeneIDi9312
      KEGGihsa:9312
      UCSCiuc003xzb.3 human

      Organism-specific databases

      CTDi9312
      DisGeNETi9312
      EuPathDBiHostDB:ENSG00000182674.5
      GeneCardsiKCNB2
      HGNCiHGNC:6232 KCNB2
      HPAiHPA065970
      MIMi607738 gene
      neXtProtiNX_Q92953
      OpenTargetsiENSG00000182674
      PharmGKBiPA30025
      GenAtlasiSearch...

      Phylogenomic databases

      eggNOGiKOG3713 Eukaryota
      COG1226 LUCA
      GeneTreeiENSGT00760000118981
      HOGENOMiHOG000113206
      HOVERGENiHBG052225
      InParanoidiQ92953
      KOiK04886
      OMAiKMENHLF
      OrthoDBiEOG091G0FP3
      PhylomeDBiQ92953
      TreeFamiTF313103

      Enzyme and pathway databases

      ReactomeiR-HSA-1296072 Voltage gated Potassium channels

      Miscellaneous databases

      ChiTaRSiKCNB2 human
      GeneWikiiKCNB2
      GenomeRNAii9312
      PROiPR:Q92953
      SOURCEiSearch...

      Gene expression databases

      BgeeiENSG00000182674 Expressed in 69 organ(s), highest expression level in buccal mucosa cell
      CleanExiHS_KCNB2
      GenevisibleiQ92953 HS

      Family and domain databases

      Gene3Di1.20.120.350, 1 hit
      InterProiView protein in InterPro
      IPR000210 BTB/POZ_dom
      IPR005821 Ion_trans_dom
      IPR003968 K_chnl_volt-dep_Kv
      IPR003973 K_chnl_volt-dep_Kv2
      IPR005826 K_chnl_volt-dep_Kv2.2
      IPR011333 SKP1/BTB/POZ_sf
      IPR003131 T1-type_BTB
      IPR028325 VG_K_chnl
      IPR027359 Volt_channel_dom_sf
      PANTHERiPTHR11537 PTHR11537, 1 hit
      PfamiView protein in Pfam
      PF02214 BTB_2, 1 hit
      PF00520 Ion_trans, 1 hit
      PF03521 Kv2channel, 1 hit
      PRINTSiPR00169 KCHANNEL
      PR01515 KV22CHANNEL
      PR01491 KVCHANNEL
      PR01495 SHABCHANNEL
      SMARTiView protein in SMART
      SM00225 BTB, 1 hit
      SUPFAMiSSF54695 SSF54695, 1 hit
      ProtoNetiSearch...

      Entry informationi

      Entry nameiKCNB2_HUMAN
      AccessioniPrimary (citable) accession number: Q92953
      Secondary accession number(s): Q7Z7D0, Q9BXD3
      Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
      Last sequence update: October 25, 2002
      Last modified: September 12, 2018
      This is version 164 of the entry and version 2 of the sequence. See complete history.
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program
      DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Reference proteome

      Documents

      1. Human chromosome 8
        Human chromosome 8: entries, gene names and cross-references to MIM
      2. Human entries with polymorphisms or disease mutations
        List of human entries with polymorphisms or disease mutations
      3. SIMILARITY comments
        Index of protein domains and families
      4. Human polymorphisms and disease mutations
        Index of human polymorphisms and disease mutations
      5. MIM cross-references
        Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
      UniProt is an ELIXIR core data resource
      Main funding by: National Institutes of Health

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