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Protein

Far upstream element-binding protein 2

Gene

KHSRP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.By similarity3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • mRNA 3'-UTR AU-rich region binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-380994 ATF4 activates genes
R-HSA-450604 KSRP (KHSRP) binds and destabilizes mRNA
SIGNORiQ92945

Names & Taxonomyi

Protein namesi
Recommended name:
Far upstream element-binding protein 2
Short name:
FUSE-binding protein 2
Alternative name(s):
KH type-splicing regulatory protein
Short name:
KSRP
p75
Gene namesi
Name:KHSRP
Synonyms:FUBP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000088247.15
HGNCiHGNC:6316 KHSRP
MIMi603445 gene
neXtProtiNX_Q92945

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8570
OpenTargetsiENSG00000088247
PharmGKBiPA30097

Chemistry databases

ChEMBLiCHEMBL1795105

Polymorphism and mutation databases

BioMutaiKHSRP
DMDMi313104306

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000501372 – 711Far upstream element-binding protein 2Add BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei40Omega-N-methylarginineBy similarity1
Modified residuei87N6-acetyllysineBy similarity1
Modified residuei99PhosphoserineCombined sources1
Modified residuei100PhosphothreonineCombined sources1
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei125PhosphoserineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei184PhosphoserineCombined sources1
Modified residuei193PhosphoserineCombined sources1 Publication1
Modified residuei274PhosphoserineCombined sources1
Modified residuei411Omega-N-methylarginineCombined sources1
Modified residuei413Omega-N-methylarginineCombined sources1
Modified residuei415Omega-N-methylarginineCombined sources1
Modified residuei442Omega-N-methylarginineCombined sources1
Modified residuei480PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ92945
MaxQBiQ92945
PaxDbiQ92945
PeptideAtlasiQ92945
PRIDEiQ92945
ProteomicsDBi75616

2D gel databases

REPRODUCTION-2DPAGEiQ92945

PTM databases

iPTMnetiQ92945
PhosphoSitePlusiQ92945
SwissPalmiQ92945

Miscellaneous databases

PMAP-CutDBiQ92945

Expressioni

Tissue specificityi

Detected in neural and non-neural cell lines.

Gene expression databases

BgeeiENSG00000088247 Expressed in 236 organ(s), highest expression level in testis
CleanExiHS_KHSRP
ExpressionAtlasiQ92945 baseline and differential
GenevisibleiQ92945 HS

Organism-specific databases

HPAiHPA034739
HPA056518

Interactioni

Subunit structurei

Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRPH1 (PubMed:11003644). Interacts with PARN (PubMed:15175153). Interacts with PQBP1 (PubMed:21933836).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DICER1Q9UPY3-13EBI-1049099,EBI-15569571

Protein-protein interaction databases

BioGridi114139, 88 interactors
DIPiDIP-48484N
ELMiQ92945
IntActiQ92945, 37 interactors
MINTiQ92945
STRINGi9606.ENSP00000381216

Structurei

Secondary structure

1711
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92945
SMRiQ92945
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92945

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini144 – 208KH 1PROSITE-ProRule annotationAdd BLAST65
Domaini233 – 299KH 2PROSITE-ProRule annotationAdd BLAST67
Domaini322 – 386KH 3PROSITE-ProRule annotationAdd BLAST65
Domaini424 – 491KH 4PROSITE-ProRule annotationAdd BLAST68
Repeati571 – 5821Add BLAST12
Repeati617 – 6282Add BLAST12
Repeati643 – 6543Add BLAST12
Repeati673 – 6844Add BLAST12

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni571 – 6844 X 12 AA imperfect repeatsAdd BLAST114

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 67Gly/Pro-richAdd BLAST61
Compositional biasi68 – 496Gly-richAdd BLAST429
Compositional biasi498 – 612Ala/Gly/Pro-richAdd BLAST115

Domaini

KH domains KH 3 and KH 4 behave as independent binding modules and can interact with different regions of the AU-rich RNA targets of degradation.

Sequence similaritiesi

Belongs to the KHSRP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1676 Eukaryota
ENOG410XZYE LUCA
GeneTreeiENSGT00730000110664
HOGENOMiHOG000231552
HOVERGENiHBG000625
InParanoidiQ92945
KOiK13210
PhylomeDBiQ92945
TreeFamiTF313654

Family and domain databases

Gene3Di3.30.1370.10, 4 hits
InterProiView protein in InterPro
IPR015096 FUBP_C
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
PfamiView protein in Pfam
PF09005 DUF1897, 2 hits
PF00013 KH_1, 4 hits
SMARTiView protein in SMART
SM00322 KH, 4 hits
SUPFAMiSSF54791 SSF54791, 4 hits
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

Q92945-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDYSTGGPP PGPPPPAGGG GGAGGAGGGP PPGPPGAGDR GGGGPGGGGP
60 70 80 90 100
GGGSAGGPSQ PPGGGGPGIR KDAFADAVQR ARQIAAKIGG DAATTVNNST
110 120 130 140 150
PDFGFGGQKR QLEDGDQPES KKLASQGDSI SSQLGPIHPP PRTSMTEEYR
160 170 180 190 200
VPDGMVGLII GRGGEQINKI QQDSGCKVQI SPDSGGLPER SVSLTGAPES
210 220 230 240 250
VQKAKMMLDD IVSRGRGGPP GQFHDNANGG QNGTVQEIMI PAGKAGLVIG
260 270 280 290 300
KGGETIKQLQ ERAGVKMILI QDGSQNTNVD KPLRIIGDPY KVQQACEMVM
310 320 330 340 350
DILRERDQGG FGDRNEYGSR IGGGIDVPVP RHSVGVVIGR SGEMIKKIQN
360 370 380 390 400
DAGVRIQFKQ DDGTGPEKIA HIMGPPDRCE HAARIINDLL QSLRSGPPGP
410 420 430 440 450
PGGPGMPPGG RGRGRGQGNW GPPGGEMTFS IPTHKCGLVI GRGGENVKAI
460 470 480 490 500
NQQTGAFVEI SRQLPPNGDP NFKLFIIRGS PQQIDHAKQL IEEKIEGPLC
510 520 530 540 550
PVGPGPGGPG PAGPMGPFNP GPFNQGPPGA PPHAGGPPPH QYPPQGWGNT
560 570 580 590 600
YPQWQPPAPH DPSKAAAAAA DPNAAWAAYY SHYYQQPPGP VPGPAPAPAA
610 620 630 640 650
PPAQGEPPQP PPTGQSDYTK AWEEYYKKIG QQPQQPGAPP QQDYTKAWEE
660 670 680 690 700
YYKKQAQVAT GGGPGAPPGS QPDYSAAWAE YYRQQAAYYG QTPGPGGPQP
710
PPTQQGQQQA Q
Length:711
Mass (Da):73,115
Last modified:November 30, 2010 - v4
Checksum:iAB0B7C0B5B938114
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WTP3A0A087WTP3_HUMAN
Far upstream element-binding protei...
KHSRP
711Annotation score:
M0R3J3M0R3J3_HUMAN
Far upstream element-binding protei...
KHSRP
269Annotation score:
M0QYH3M0QYH3_HUMAN
Far upstream element-binding protei...
KHSRP
169Annotation score:
M0QXW7M0QXW7_HUMAN
Far upstream element-binding protei...
KHSRP
116Annotation score:
M0R0I5M0R0I5_HUMAN
Far upstream element-binding protei...
KHSRP
279Annotation score:
M0QYG1M0QYG1_HUMAN
Far upstream element-binding protei...
KHSRP
114Annotation score:
M0R0C6M0R0C6_HUMAN
Far upstream element-binding protei...
KHSRP
176Annotation score:
M0R251M0R251_HUMAN
Far upstream element-binding protei...
KHSRP
79Annotation score:
M0R263M0R263_HUMAN
Far upstream element-binding protei...
KHSRP
143Annotation score:

Sequence cautioni

The sequence AAC50892 differs from that shown. Reason: Frameshift at positions 304, 309, 466 and 473.Curated
The sequence AAH85004 differs from that shown. Aberrant splicing.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46G → C in AAB53222 (PubMed:9136930).Curated1
Sequence conflicti46G → C in AAD29861 (PubMed:10087204).Curated1
Sequence conflicti96V → G in AAC50892 (PubMed:8940189).Curated1
Sequence conflicti406 – 407MP → I in AAC50892 (PubMed:8940189).Curated2
Sequence conflicti422Missing in AAC50892 (PubMed:8940189).Curated1
Sequence conflicti487 – 488AK → CR in AAC50892 (PubMed:8940189).Curated2
Sequence conflicti694 – 696GPG → VP in AAC50892 (PubMed:8940189).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94832 mRNA Translation: AAB53222.1
AC011491 Genomic DNA No translation available.
AC011539 Genomic DNA No translation available.
BC085004 mRNA Translation: AAH85004.1 Sequence problems.
AF093747, AF093745 Genomic DNA Translation: AAD29861.1
AF093748 Genomic DNA Translation: AAD29862.1
U69126 mRNA Translation: AAC50892.1 Frameshift.
AB209662 mRNA Translation: BAD92899.1
CCDSiCCDS45936.1
RefSeqiNP_003676.2, NM_003685.2
UniGeneiHs.727344

Genome annotation databases

EnsembliENST00000398148; ENSP00000381216; ENSG00000088247
GeneIDi8570
KEGGihsa:8570
UCSCiuc002mer.5 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94832 mRNA Translation: AAB53222.1
AC011491 Genomic DNA No translation available.
AC011539 Genomic DNA No translation available.
BC085004 mRNA Translation: AAH85004.1 Sequence problems.
AF093747, AF093745 Genomic DNA Translation: AAD29861.1
AF093748 Genomic DNA Translation: AAD29862.1
U69126 mRNA Translation: AAC50892.1 Frameshift.
AB209662 mRNA Translation: BAD92899.1
CCDSiCCDS45936.1
RefSeqiNP_003676.2, NM_003685.2
UniGeneiHs.727344

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HH2NMR-A424-525[»]
2HH3NMR-A318-418[»]
2JVZNMR-A233-396[»]
2OPUNMR-A130-218[»]
2OPVNMR-A221-305[»]
4B8TNMR-A317-418[»]
ProteinModelPortaliQ92945
SMRiQ92945
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114139, 88 interactors
DIPiDIP-48484N
ELMiQ92945
IntActiQ92945, 37 interactors
MINTiQ92945
STRINGi9606.ENSP00000381216

Chemistry databases

ChEMBLiCHEMBL1795105

PTM databases

iPTMnetiQ92945
PhosphoSitePlusiQ92945
SwissPalmiQ92945

Polymorphism and mutation databases

BioMutaiKHSRP
DMDMi313104306

2D gel databases

REPRODUCTION-2DPAGEiQ92945

Proteomic databases

EPDiQ92945
MaxQBiQ92945
PaxDbiQ92945
PeptideAtlasiQ92945
PRIDEiQ92945
ProteomicsDBi75616

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398148; ENSP00000381216; ENSG00000088247
GeneIDi8570
KEGGihsa:8570
UCSCiuc002mer.5 human

Organism-specific databases

CTDi8570
DisGeNETi8570
EuPathDBiHostDB:ENSG00000088247.15
GeneCardsiKHSRP
H-InvDBiHIX0040083
HGNCiHGNC:6316 KHSRP
HPAiHPA034739
HPA056518
MIMi603445 gene
neXtProtiNX_Q92945
OpenTargetsiENSG00000088247
PharmGKBiPA30097
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1676 Eukaryota
ENOG410XZYE LUCA
GeneTreeiENSGT00730000110664
HOGENOMiHOG000231552
HOVERGENiHBG000625
InParanoidiQ92945
KOiK13210
PhylomeDBiQ92945
TreeFamiTF313654

Enzyme and pathway databases

ReactomeiR-HSA-380994 ATF4 activates genes
R-HSA-450604 KSRP (KHSRP) binds and destabilizes mRNA
SIGNORiQ92945

Miscellaneous databases

ChiTaRSiKHSRP human
EvolutionaryTraceiQ92945
GeneWikiiKHSRP
GenomeRNAii8570
PMAP-CutDBiQ92945
PROiPR:Q92945
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000088247 Expressed in 236 organ(s), highest expression level in testis
CleanExiHS_KHSRP
ExpressionAtlasiQ92945 baseline and differential
GenevisibleiQ92945 HS

Family and domain databases

Gene3Di3.30.1370.10, 4 hits
InterProiView protein in InterPro
IPR015096 FUBP_C
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
PfamiView protein in Pfam
PF09005 DUF1897, 2 hits
PF00013 KH_1, 4 hits
SMARTiView protein in SMART
SM00322 KH, 4 hits
SUPFAMiSSF54791 SSF54791, 4 hits
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiFUBP2_HUMAN
AccessioniPrimary (citable) accession number: Q92945
Secondary accession number(s): O00301
, Q59EZ9, Q5U4P6, Q9UNT5, Q9UQH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 30, 2010
Last modified: November 7, 2018
This is version 179 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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