UniProtKB - Q92879 (CELF1_HUMAN)
CUGBP Elav-like family member 1
CELF1
Functioni
RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity).
Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity).
Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA (PubMed:28431233).
By similarity9 PublicationsGO - Molecular functioni
- BRE binding Source: UniProtKB
- mRNA 3'-UTR binding Source: ARUK-UCL
- mRNA binding Source: BHF-UCL
- pre-mRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
- translation repressor activity, mRNA regulatory element binding Source: UniProtKB
GO - Biological processi
- embryo development ending in birth or egg hatching Source: UniProtKB
- germ cell development Source: UniProtKB
- mRNA destabilization Source: ARUK-UCL
- mRNA processing Source: ProtInc
- mRNA splice site selection Source: UniProtKB
- negative regulation of cell population proliferation Source: ARUK-UCL
- negative regulation of gene expression Source: ARUK-UCL
- positive regulation of cell death Source: ARUK-UCL
- positive regulation of gene expression Source: ARUK-UCL
- posttranscriptional gene silencing Source: ARUK-UCL
- regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
- regulation of inflammatory response Source: ARUK-UCL
- regulation of RNA splicing Source: UniProtKB
- RNA interference Source: UniProtKB
Keywordsi
Molecular function | Activator, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | Q92879 |
SignaLinki | Q92879 |
SIGNORi | Q92879 |
Names & Taxonomyi
Protein namesi | Recommended name: CUGBP Elav-like family member 1Short name: CELF-1 Alternative name(s): 50 kDa nuclear polyadenylated RNA-binding protein Bruno-like protein 2 CUG triplet repeat RNA-binding protein 1 Short name: CUG-BP1 CUG-BP- and ETR-3-like factor 1 Deadenylation factor CUG-BP Embryo deadenylation element-binding protein homolog Short name: EDEN-BP homolog RNA-binding protein BRUNOL-2 |
Gene namesi | Name:CELF1 Synonyms:BRUNOL2, CUGBP, CUGBP1, NAB50 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2549, CELF1 |
MIMi | 601074, gene |
neXtProti | NX_Q92879 |
VEuPathDBi | HostDB:ENSG00000149187 |
Subcellular locationi
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- perinucleolar compartment Source: ARUK-UCL
Other locations
- cytoplasm Source: GO_Central
- cytoplasmic stress granule Source: ARUK-UCL
- membrane Source: UniProtKB
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 63 | F → L: Does not reduce RNA-binding; when associated with D-331 and F-472. Abolishes ARE/EDEN-dependent deadenylation; when associated with D-331 and F-472. 1 Publication | 1 | |
Mutagenesisi | 331 | G → D: Does not reduce RNA-binding; when associated with L-63 and F-472. Abolishes ARE/EDEN-dependent deadenylation; when associated with D-331 and F-472. 1 Publication | 1 | |
Mutagenesisi | 472 | L → F: Does not reduce RNA-binding; when associated with L-63 and D-331. Abolishes ARE/EDEN-dependent deadenylation; when associated with D-331 and F-472. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10658 |
NIAGADSi | ENSG00000149187 |
OpenTargetsi | ENSG00000149187 |
PharmGKBi | PA27045 |
Miscellaneous databases
Pharosi | Q92879, Tbio |
Genetic variation databases
BioMutai | CELF1 |
DMDMi | 17374605 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081538 | 1 – 486 | CUGBP Elav-like family member 1Add BLAST | 486 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 4 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 109 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 179 | PhosphoserineCombined sources | 1 | |
Modified residuei | 302 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q92879 |
jPOSTi | Q92879 |
MassIVEi | Q92879 |
MaxQBi | Q92879 |
PaxDbi | Q92879 |
PeptideAtlasi | Q92879 |
PRIDEi | Q92879 |
ProteomicsDBi | 30255 75565 [Q92879-1] 75566 [Q92879-2] 75567 [Q92879-3] 75568 [Q92879-4] |
PTM databases
GlyGeni | Q92879, 7 sites, 1 O-linked glycan (7 sites) |
iPTMneti | Q92879 |
MetOSitei | Q92879 |
PhosphoSitePlusi | Q92879 |
SwissPalmi | Q92879 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000149187, Expressed in forebrain and 261 other tissues |
ExpressionAtlasi | Q92879, baseline and differential |
Genevisiblei | Q92879, HS |
Organism-specific databases
HPAi | ENSG00000149187, Low tissue specificity |
Interactioni
Subunit structurei
Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Associates with polysomes (By similarity).
Interacts with HNRNPH1; the interaction in RNA-dependent.
Interacts with PARN.
By similarity3 PublicationsBinary interactionsi
Isoform 3 [Q92879-3]
With | #Exp. | IntAct |
---|---|---|
TARDBP [Q13148] | 3 | EBI-21370617,EBI-372899 |
Protein-protein interaction databases
BioGRIDi | 115901, 264 interactors |
IntActi | Q92879, 36 interactors |
MINTi | Q92879 |
STRINGi | 9606.ENSP00000435926 |
Miscellaneous databases
RNActi | Q92879, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q92879 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q92879 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 99 | RRM 1PROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 108 – 188 | RRM 2PROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 401 – 479 | RRM 3PROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 277 – 309 | DisorderedSequence analysisAdd BLAST | 33 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0144, Eukaryota |
GeneTreei | ENSGT00940000158970 |
HOGENOMi | CLU_015367_0_2_1 |
InParanoidi | Q92879 |
PhylomeDBi | Q92879 |
TreeFami | TF314924 |
Family and domain databases
CDDi | cd12631, RRM1_CELF1_2_Bruno, 1 hit cd12634, RRM2_CELF1_2, 1 hit cd12638, RRM3_CELF1_2, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
IDEALi | IID00490 |
InterProi | View protein in InterPro IPR034196, CELF1/2_RRM1 IPR034198, CELF1/2_RRM2 IPR034199, CELF1/2_RRM3 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNGTLDHPDQ PDLDAIKMFV GQVPRTWSEK DLRELFEQYG AVYEINVLRD
60 70 80 90 100
RSQNPPQSKG CCFVTFYTRK AALEAQNALH NMKVLPGMHH PIQMKPADSE
110 120 130 140 150
KNNAVEDRKL FIGMISKKCT ENDIRVMFSS FGQIEECRIL RGPDGLSRGC
160 170 180 190 200
AFVTFTTRAM AQTAIKAMHQ AQTMEGCSSP MVVKFADTQK DKEQKRMAQQ
210 220 230 240 250
LQQQMQQISA ASVWGNLAGL NTLGPQYLAL YLQLLQQTAS SGNLNTLSSL
260 270 280 290 300
HPMGGLNAMQ LQNLAALAAA ASAAQNTPSG TNALTTSSSP LSVLTSSGSS
310 320 330 340 350
PSSSSSNSVN PIASLGALQT LAGATAGLNV GSLAGMAALN GGLGSSGLSN
360 370 380 390 400
GTGSTMEALT QAYSGIQQYA AAALPTLYNQ NLLTQQSIGA AGSQKEGPEG
410 420 430 440 450
ANLFIYHLPQ EFGDQDLLQM FMPFGNVVSA KVFIDKQTNL SKCFGFVSYD
460 470 480
NPVSAQAAIQ SMNGFQIGMK RLKVQLKRSK NDSKPY
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG5EA30 | G5EA30_HUMAN | CUG triplet repeat, RNA binding pro... | CELF1 CUGBP1, hCG_25183 | 514 | Annotation score: | ||
E9PSH0 | E9PSH0_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 95 | Annotation score: | ||
E9PQK4 | E9PQK4_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 87 | Annotation score: | ||
F5H0D8 | F5H0D8_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 103 | Annotation score: | ||
E9PKA1 | E9PKA1_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 56 | Annotation score: | ||
E9PKU1 | E9PKU1_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 114 | Annotation score: | ||
F5H4Y5 | F5H4Y5_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 78 | Annotation score: | ||
F5H3J7 | F5H3J7_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 37 | Annotation score: | ||
F5H7M7 | F5H7M7_HUMAN | CUGBP Elav-like family member 1 | CELF1 | 14 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045043 | 1 – 17 | Missing in isoform 5. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_026787 | 1 | M → MAAFKLDFLPEMMVDHCSLN SSPVSKKM in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_026788 | 104 | Missing in isoform 4, isoform 5 and isoform 6. 2 Publications | 1 | |
Alternative sequenceiVSP_005784 | 231 – 234 | Missing in isoform 2 and isoform 3. 3 Publications | 4 | |
Alternative sequenceiVSP_005785 | 297 | S → SA in isoform 3. 2 Publications | 1 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000310513; ENSP00000308386; ENSG00000149187 [Q92879-2] ENST00000358597; ENSP00000351409; ENSG00000149187 ENST00000361904; ENSP00000354639; ENSG00000149187 [Q92879-3] ENST00000395290; ENSP00000378705; ENSG00000149187 [Q92879-6] ENST00000395292; ENSP00000378706; ENSG00000149187 [Q92879-3] ENST00000532048; ENSP00000435926; ENSG00000149187 [Q92879-4] |
GeneIDi | 10658 |
KEGGi | hsa:10658 |
UCSCi | uc001nfk.3, human [Q92879-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CPZ | NMR | - | A | 383-484 | [»] | |
2DHS | NMR | - | A | 1-187 | [»] | |
2RQ4 | NMR | - | A | 383-484 | [»] | |
2RQC | NMR | - | A | 383-484 | [»] | |
3NMR | X-ray | 1.85 | A | 14-187 | [»] | |
3NNA | X-ray | 1.90 | A | 14-187 | [»] | |
3NNC | X-ray | 2.20 | A | 14-187 | [»] | |
3NNH | X-ray | 2.75 | A/B/C/D | 14-100 | [»] | |
SMRi | Q92879 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115901, 264 interactors |
IntActi | Q92879, 36 interactors |
MINTi | Q92879 |
STRINGi | 9606.ENSP00000435926 |
PTM databases
GlyGeni | Q92879, 7 sites, 1 O-linked glycan (7 sites) |
iPTMneti | Q92879 |
MetOSitei | Q92879 |
PhosphoSitePlusi | Q92879 |
SwissPalmi | Q92879 |
Genetic variation databases
BioMutai | CELF1 |
DMDMi | 17374605 |
Proteomic databases
EPDi | Q92879 |
jPOSTi | Q92879 |
MassIVEi | Q92879 |
MaxQBi | Q92879 |
PaxDbi | Q92879 |
PeptideAtlasi | Q92879 |
PRIDEi | Q92879 |
ProteomicsDBi | 30255 75565 [Q92879-1] 75566 [Q92879-2] 75567 [Q92879-3] 75568 [Q92879-4] |
Protocols and materials databases
Antibodypediai | 4239, 392 antibodies from 34 providers |
DNASUi | 10658 |
Genome annotation databases
Ensembli | ENST00000310513; ENSP00000308386; ENSG00000149187 [Q92879-2] ENST00000358597; ENSP00000351409; ENSG00000149187 ENST00000361904; ENSP00000354639; ENSG00000149187 [Q92879-3] ENST00000395290; ENSP00000378705; ENSG00000149187 [Q92879-6] ENST00000395292; ENSP00000378706; ENSG00000149187 [Q92879-3] ENST00000532048; ENSP00000435926; ENSG00000149187 [Q92879-4] |
GeneIDi | 10658 |
KEGGi | hsa:10658 |
UCSCi | uc001nfk.3, human [Q92879-1] |
Organism-specific databases
CTDi | 10658 |
DisGeNETi | 10658 |
GeneCardsi | CELF1 |
HGNCi | HGNC:2549, CELF1 |
HPAi | ENSG00000149187, Low tissue specificity |
MIMi | 601074, gene |
neXtProti | NX_Q92879 |
NIAGADSi | ENSG00000149187 |
OpenTargetsi | ENSG00000149187 |
PharmGKBi | PA27045 |
VEuPathDBi | HostDB:ENSG00000149187 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0144, Eukaryota |
GeneTreei | ENSGT00940000158970 |
HOGENOMi | CLU_015367_0_2_1 |
InParanoidi | Q92879 |
PhylomeDBi | Q92879 |
TreeFami | TF314924 |
Enzyme and pathway databases
PathwayCommonsi | Q92879 |
SignaLinki | Q92879 |
SIGNORi | Q92879 |
Miscellaneous databases
BioGRID-ORCSi | 10658, 35 hits in 1060 CRISPR screens |
ChiTaRSi | CELF1, human |
EvolutionaryTracei | Q92879 |
GeneWikii | CUGBP1 |
GenomeRNAii | 10658 |
Pharosi | Q92879, Tbio |
PROi | PR:Q92879 |
RNActi | Q92879, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000149187, Expressed in forebrain and 261 other tissues |
ExpressionAtlasi | Q92879, baseline and differential |
Genevisiblei | Q92879, HS |
Family and domain databases
CDDi | cd12631, RRM1_CELF1_2_Bruno, 1 hit cd12634, RRM2_CELF1_2, 1 hit cd12638, RRM3_CELF1_2, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
IDEALi | IID00490 |
InterProi | View protein in InterPro IPR034196, CELF1/2_RRM1 IPR034198, CELF1/2_RRM2 IPR034199, CELF1/2_RRM3 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CELF1_HUMAN | |
Accessioni | Q92879Primary (citable) accession number: Q92879 Secondary accession number(s): B4E2U5 Q9NR06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | October 18, 2001 | |
Last modified: | February 23, 2022 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families