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Protein

Histone acetyltransferase KAT2B

Gene

KAT2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, PLK4 and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed:29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed:27796307).7 Publications
(Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.1 Publication

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei570Proton donor/acceptor1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Acyltransferase, Transferase
Biological processBiological rhythms, Cell cycle, Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

BRENDAi2.3.1.48 2681
ReactomeiR-HSA-1912408 Pre-NOTCH Transcription and Translation
R-HSA-2032785 YAP1- and WWTR1 (TAZ)-stimulated gene expression
R-HSA-210744 Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells
R-HSA-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-HSA-2644606 Constitutive Signaling by NOTCH1 PEST Domain Mutants
R-HSA-2894862 Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants
R-HSA-3214847 HATs acetylate histones
R-HSA-350054 Notch-HLH transcription pathway
R-HSA-5250924 B-WICH complex positively regulates rRNA expression
R-HSA-5578768 Physiological factors
R-HSA-5689901 Metalloprotease DUBs
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-HSA-8941856 RUNX3 regulates NOTCH signaling
R-HSA-9013508 NOTCH3 Intracellular Domain Regulates Transcription
R-HSA-9013695 NOTCH4 Intracellular Domain Regulates Transcription
R-HSA-9018519 Estrogen-dependent gene expression
SIGNORiQ92831

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase KAT2B (EC:2.3.1.481 Publication)
Alternative name(s):
Histone acetyltransferase PCAF1 Publication
Short name:
Histone acetylase PCAF1 Publication
Lysine acetyltransferase 2B1 Publication
P300/CBP-associated factor1 Publication
Short name:
P/CAF1 Publication
Gene namesi
Name:KAT2B1 PublicationImported
Synonyms:PCAF1 Publication
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000114166.7
HGNCiHGNC:8638 KAT2B
MIMi602303 gene
neXtProtiNX_Q92831

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in KAT2B has been found in a patient with isolated coloboma, a defect of the eye characterized by the absence of ocular structures due to abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Isolated colobomas may be associated with an abnormally small eye (microphthalmia) or small cornea.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi752V → A: Reduced acetyl-lysine binding. 1 Publication1
Mutagenesisi760Y → A: Reduced acetyl-lysine binding. 1 Publication1
Mutagenesisi802Y → A: Reduced acetyl-lysine binding. 1 Publication1
Mutagenesisi809Y → A: Complete loss of acetyl-lysine binding. 1 Publication1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi8850
OpenTargetsiENSG00000114166
PharmGKBiPA162392705

Chemistry databases

ChEMBLiCHEMBL5500
DrugBankiDB08186 (3E)-4-(1-METHYL-1H-INDOL-3-YL)BUT-3-EN-2-ONE
DB01992 Coenzyme A
DB08291 N-(3-AMINOPROPYL)-2-NITROBENZENAMINE
GuidetoPHARMACOLOGYi2737

Polymorphism and mutation databases

BioMutaiKAT2B
DMDMi83287776

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002112081 – 832Histone acetyltransferase KAT2BAdd BLAST832

Proteomic databases

EPDiQ92831
MaxQBiQ92831
PaxDbiQ92831
PeptideAtlasiQ92831
PRIDEiQ92831
ProteomicsDBi75508

2D gel databases

REPRODUCTION-2DPAGEiQ92831

PTM databases

CarbonylDBiQ92831
iPTMnetiQ92831
PhosphoSitePlusiQ92831

Expressioni

Tissue specificityi

Ubiquitously expressed but most abundant in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000114166 Expressed in 230 organ(s), highest expression level in corpus callosum
CleanExiHS_KAT2B
GenevisibleiQ92831 HS

Organism-specific databases

HPAiCAB004526
HPA055839

Interactioni

Subunit structurei

Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity (By similarity). Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Interacts with NPAS2, ARNTL/BMAL1 and CLOCK. Interacts with BCAS3. Interacts with CEBPB (PubMed:17301242). Interacts with NR4A3 (By similarity). Interacts with NFATC2 (By similarity). Interacts with TBX5 (PubMed:29174768). Interacts with PLK4 (PubMed:27796307).By similarity17 Publications
(Microbial infection) Interacts with and acetylates HIV-1 Tat.1 Publication
(Microbial infection) Interacts with HTLV-1 Tax.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114375, 190 interactors
ComplexPortaliCPX-1004 PCAF-containing ATAC complex
CPX-989 PCAF histone acetylase complex
CORUMiQ92831
DIPiDIP-29778N
IntActiQ92831, 40 interactors
MINTiQ92831
STRINGi9606.ENSP00000263754

Chemistry databases

BindingDBiQ92831

Structurei

Secondary structure

1832
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92831
SMRiQ92831
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92831

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini503 – 651N-acetyltransferasePROSITE-ProRule annotationBy similarityAdd BLAST149
Domaini740 – 810BromoPROSITE-ProRule annotationCuratedAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni574 – 576Acetyl-CoA binding1 Publication3
Regioni581 – 587Acetyl-CoA binding1 Publication7
Regioni612 – 615Acetyl-CoA binding1 Publication4

Domaini

(Microbial infection) The bromodomain mediates binding to HIV-1 Tat.1 Publication

Sequence similaritiesi

Keywords - Domaini

Bromodomain

Phylogenomic databases

eggNOGiKOG1472 Eukaryota
COG5076 LUCA
GeneTreeiENSGT00920000149009
HOGENOMiHOG000007151
InParanoidiQ92831
KOiK06062
OMAiQVIRFPM
OrthoDBiEOG091G03ZO
PhylomeDBiQ92831
TreeFamiTF105399

Family and domain databases

Gene3Di1.20.920.10, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR037800 GCN5
IPR016376 GCN5/PCAF
IPR000182 GNAT_dom
IPR009464 PCAF_N
PANTHERiPTHR22880:SF124 PTHR22880:SF124, 1 hit
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
PF00439 Bromodomain, 1 hit
PF06466 PCAF_N, 1 hit
PIRSFiPIRSF003048 Histone_acetylase_PCAF, 1 hit
PRINTSiPR00503 BROMODOMAIN
SMARTiView protein in SMART
SM00297 BROMO, 1 hit
SUPFAMiSSF47370 SSF47370, 1 hit
SSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS00633 BROMODOMAIN_1, 1 hit
PS50014 BROMODOMAIN_2, 1 hit
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q92831-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEAGGAGPG GCGAGAGAGA GPGALPPQPA ALPPAPPQGS PCAAAAGGSG
60 70 80 90 100
ACGPATAVAA AGTAEGPGGG GSARIAVKKA QLRSAPRAKK LEKLGVYSAC
110 120 130 140 150
KAEESCKCNG WKNPNPSPTP PRADLQQIIV SLTESCRSCS HALAAHVSHL
160 170 180 190 200
ENVSEEEMNR LLGIVLDVEY LFTCVHKEED ADTKQVYFYL FKLLRKSILQ
210 220 230 240 250
RGKPVVEGSL EKKPPFEKPS IEQGVNNFVQ YKFSHLPAKE RQTIVELAKM
260 270 280 290 300
FLNRINYWHL EAPSQRRLRS PNDDISGYKE NYTRWLCYCN VPQFCDSLPR
310 320 330 340 350
YETTQVFGRT LLRSVFTVMR RQLLEQARQE KDKLPLEKRT LILTHFPKFL
360 370 380 390 400
SMLEEEVYSQ NSPIWDQDFL SASSRTSQLG IQTVINPPPV AGTISYNSTS
410 420 430 440 450
SSLEQPNAGS SSPACKASSG LEANPGEKRK MTDSHVLEEA KKPRVMGDIP
460 470 480 490 500
MELINEVMST ITDPAAMLGP ETNFLSAHSA RDEAARLEER RGVIEFHVVG
510 520 530 540 550
NSLNQKPNKK ILMWLVGLQN VFSHQLPRMP KEYITRLVFD PKHKTLALIK
560 570 580 590 600
DGRVIGGICF RMFPSQGFTE IVFCAVTSNE QVKGYGTHLM NHLKEYHIKH
610 620 630 640 650
DILNFLTYAD EYAIGYFKKQ GFSKEIKIPK TKYVGYIKDY EGATLMGCEL
660 670 680 690 700
NPRIPYTEFS VIIKKQKEII KKLIERKQAQ IRKVYPGLSC FKDGVRQIPI
710 720 730 740 750
ESIPGIRETG WKPSGKEKSK EPRDPDQLYS TLKSILQQVK SHQSAWPFME
760 770 780 790 800
PVKRTEAPGY YEVIRFPMDL KTMSERLKNR YYVSKKLFMA DLQRVFTNCK
810 820 830
EYNPPESEYY KCANILEKFF FSKIKEAGLI DK
Length:832
Mass (Da):93,013
Last modified:May 10, 2005 - v3
Checksum:i72F516E8BC00CC0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti804 – 805PP → AA in AAC50890 (PubMed:8684459).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_079852130V → G Found in a patient with isolated coloboma; unknown pathological significance. 1 Publication1
Natural variantiVAR_034372386N → S. Corresponds to variant dbSNP:rs17006625Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57317 mRNA Translation: AAC50890.2
BC060823 mRNA Translation: AAH60823.1
BC070075 mRNA Translation: AAH70075.1
CCDSiCCDS2634.1
PIRiS71788
RefSeqiNP_003875.3, NM_003884.4
UniGeneiHs.533055

Genome annotation databases

EnsembliENST00000263754; ENSP00000263754; ENSG00000114166
GeneIDi8850
KEGGihsa:8850
UCSCiuc003cbq.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKAT2B_HUMAN
AccessioniPrimary (citable) accession number: Q92831
Secondary accession number(s): Q6NSK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: May 10, 2005
Last modified: September 12, 2018
This is version 200 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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