UniProtKB - Q92786 (PROX1_HUMAN)
Protein
Prospero homeobox protein 1
Gene
PROX1
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.1 Publication1 Publication
GO - Molecular functioni
- DNA binding Source: BHF-UCL
- DNA binding domain binding Source: BHF-UCL
- DNA-binding transcription factor activity Source: GO_Central
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: BHF-UCL
- LBD domain binding Source: BHF-UCL
- nuclear receptor binding Source: BHF-UCL
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
- transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
GO - Biological processi
- acinar cell differentiation Source: Ensembl
- aorta smooth muscle tissue morphogenesis Source: BHF-UCL
- atrial cardiac muscle tissue morphogenesis Source: BHF-UCL
- brain development Source: BHF-UCL
- branching involved in pancreas morphogenesis Source: Ensembl
- cell fate determination Source: Ensembl
- cerebellar granule cell differentiation Source: BHF-UCL
- circadian rhythm Source: Ensembl
- dentate gyrus development Source: BHF-UCL
- dorsal spinal cord development Source: BHF-UCL
- embryonic retina morphogenesis in camera-type eye Source: BHF-UCL
- endocardium formation Source: BHF-UCL
- hepatocyte cell migration Source: Ensembl
- hepatocyte differentiation Source: BHF-UCL
- hepatocyte proliferation Source: Ensembl
- kidney development Source: BHF-UCL
- lens development in camera-type eye Source: BHF-UCL
- lens fiber cell morphogenesis Source: BHF-UCL
- liver development Source: BHF-UCL
- lung development Source: BHF-UCL
- lymphangiogenesis Source: BHF-UCL
- lymphatic endothelial cell differentiation Source: BHF-UCL
- negative regulation of bile acid biosynthetic process Source: BHF-UCL
- negative regulation of cell population proliferation Source: BHF-UCL
- negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
- negative regulation of transcription, DNA-templated Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- negative regulation of viral genome replication Source: BHF-UCL
- neural tube development Source: BHF-UCL
- neuronal stem cell population maintenance Source: BHF-UCL
- olfactory placode formation Source: BHF-UCL
- optic placode formation involved in camera-type eye formation Source: BHF-UCL
- otic placode formation Source: BHF-UCL
- pancreas development Source: BHF-UCL
- positive regulation of cell cycle Source: BHF-UCL
- positive regulation of cell cycle checkpoint Source: Ensembl
- positive regulation of cell population proliferation Source: BHF-UCL
- positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
- positive regulation of endothelial cell migration Source: BHF-UCL
- positive regulation of endothelial cell proliferation Source: BHF-UCL
- positive regulation of forebrain neuron differentiation Source: BHF-UCL
- positive regulation of heart growth Source: BHF-UCL
- positive regulation of neural precursor cell proliferation Source: BHF-UCL
- positive regulation of sarcomere organization Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of circadian rhythm Source: UniProtKB
- regulation of gene expression Source: BHF-UCL
- regulation of transcription involved in lymphatic endothelial cell fate commitment Source: BHF-UCL
- response to nutrient levels Source: Ensembl
- retina morphogenesis in camera-type eye Source: BHF-UCL
- skeletal muscle thin filament assembly Source: BHF-UCL
- venous blood vessel morphogenesis Source: BHF-UCL
- ventricular cardiac muscle tissue morphogenesis Source: BHF-UCL
- ventricular cardiac myofibril assembly Source: BHF-UCL
- ventricular septum morphogenesis Source: BHF-UCL
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q92786 |
SIGNORi | Q92786 |
Names & Taxonomyi
Protein namesi | Recommended name: Prospero homeobox protein 1Alternative name(s): Homeobox prospero-like protein PROX1 Short name: PROX-1 |
Gene namesi | Name:PROX1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9459, PROX1 |
MIMi | 601546, gene |
neXtProti | NX_Q92786 |
VEuPathDBi | HostDB:ENSG00000117707.15 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: RORG promotes its nuclear localization.By similarity
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 5629 |
OpenTargetsi | ENSG00000117707 |
PharmGKBi | PA33812 |
Miscellaneous databases
Pharosi | Q92786, Tbio |
Genetic variation databases
BioMutai | PROX1 |
DMDMi | 85702224 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208880 | 1 – 737 | Prospero homeobox protein 1Add BLAST | 737 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 177 | PhosphoserineCombined sources | 1 | |
Modified residuei | 179 | PhosphoserineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 291 | PhosphoserineBy similarity | 1 | |
Modified residuei | 295 | PhosphoserineCombined sources | 1 | |
Cross-linki | 324 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 511 | PhosphoserineBy similarity | 1 | |
Modified residuei | 514 | PhosphoserineBy similarity | 1 | |
Modified residuei | 557 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q92786 |
MassIVEi | Q92786 |
MaxQBi | Q92786 |
PaxDbi | Q92786 |
PeptideAtlasi | Q92786 |
PRIDEi | Q92786 |
ProteomicsDBi | 75470 |
PTM databases
iPTMneti | Q92786 |
PhosphoSitePlusi | Q92786 |
Expressioni
Tissue specificityi
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000117707, Expressed in liver and 186 other tissues |
ExpressionAtlasi | Q92786, baseline and differential |
Genevisiblei | Q92786, HS |
Organism-specific databases
HPAi | ENSG00000117707, Tissue enriched (liver) |
Interactioni
Subunit structurei
Interacts with RORA and RORG (via AF-2 motif).
By similarityBinary interactionsi
Hide detailsQ92786
With | #Exp. | IntAct |
---|---|---|
CTBP2 [P56545] | 2 | EBI-3912635,EBI-741533 |
HNF1A [P20823] | 3 | EBI-3912635,EBI-636034 |
HNF4A [P41235] | 3 | EBI-3912635,EBI-1049011 |
NR5A2 - isoform 1 [O00482-2] | 9 | EBI-3912635,EBI-9257474 |
GO - Molecular functioni
- DNA binding domain binding Source: BHF-UCL
- LBD domain binding Source: BHF-UCL
- nuclear receptor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111613, 20 interactors |
IntActi | Q92786, 12 interactors |
MINTi | Q92786 |
STRINGi | 9606.ENSP00000355925 |
Miscellaneous databases
RNActi | Q92786, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q92786 |
SMRi | Q92786 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 577 – 635 | Prospero-type homeoPROSITE-ProRule annotationAdd BLAST | 59 | |
Domaini | 636 – 735 | ProsperoPROSITE-ProRule annotationAdd BLAST | 100 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 28 | Interaction with RORGBy similarityAdd BLAST | 28 | |
Regioni | 577 – 735 | Homeo-ProsperoPROSITE-ProRule annotationAdd BLAST | 159 | |
Regioni | 723 – 729 | Essential for nuclear localization, interaction with RORG, repression of RORG transcriptional activator activityBy similarity | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 215 – 219 | Poly-Gln | 5 |
Domaini
The Prospero-type homeodomain and the adjacent Prospero domain act as a single structural unit, the Homeo-Prospero domain. The Prospero-type homeodomain is essential for repression of RORG transcriptional activator activity.PROSITE-ProRule annotationBy similarity
Sequence similaritiesi
Belongs to the Prospero homeodomain family.PROSITE-ProRule annotation
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3779, Eukaryota |
GeneTreei | ENSGT00940000154790 |
HOGENOMi | CLU_016051_0_0_1 |
InParanoidi | Q92786 |
OMAi | QEDVCSN |
OrthoDBi | 373478at2759 |
PhylomeDBi | Q92786 |
TreeFami | TF316638 |
Family and domain databases
Gene3Di | 1.10.10.500, 1 hit |
InterProi | View protein in InterPro IPR023082, Homeo_prospero_dom IPR037131, Homeo_prospero_dom_sf IPR009057, Homeobox-like_sf IPR039350, Prospero_homeodomain IPR007738, Prox1 |
PANTHERi | PTHR12198, PTHR12198, 1 hit PTHR12198:SF6, PTHR12198:SF6, 1 hit |
Pfami | View protein in Pfam PF05044, HPD, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS51818, HOMEO_PROSPERO, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q92786-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPDHDSTALL SRQTKRRRVD IGVKRTVGTA SAFFAKARAT FFSAMNPQGS
60 70 80 90 100
EQDVEYSVVQ HADGEKSNVL RKLLKRANSY EDAMMPFPGA TIISQLLKNN
110 120 130 140 150
MNKNGGTEPS FQASGLSSTG SEVHQEDICS NSSRDSPPEC LSPFGRPTMS
160 170 180 190 200
QFDMDRLCDE HLRAKRARVE NIIRGMSHSP SVALRGNENE REMAPQSVSP
210 220 230 240 250
RESYRENKRK QKLPQQQQQS FQQLVSARKE QKREERRQLK QQLEDMQKQL
260 270 280 290 300
RQLQEKFYQI YDSTDSENDE DGNLSEDSMR SEILDARAQD SVGRSDNEMC
310 320 330 340 350
ELDPGQFIDR ARALIREQEM AENKPKREGN NKERDHGPNS LQPEGKHLAE
360 370 380 390 400
TLKQELNTAM SQVVDTVVKV FSAKPSRQVP QVFPPLQIPQ ARFAVNGENH
410 420 430 440 450
NFHTANQRLQ CFGDVIIPNP LDTFGNVQMA SSTDQTEALP LVVRKNSSDQ
460 470 480 490 500
SASGPAAGGH HQPLHQSPLS ATTGFTTSTF RHPFPLPLMA YPFQSPLGAP
510 520 530 540 550
SGSFSGKDRA SPESLDLTRD TTSLRTKMSS HHLSHHPCSP AHPPSTAEGL
560 570 580 590 600
SLSLIKSECG DLQDMSEISP YSGSAMQEGL SPNHLKKAKL MFFYTRYPSS
610 620 630 640 650
NMLKTYFSDV KFNRCITSQL IKWFSNFREF YYIQMEKYAR QAINDGVTST
660 670 680 690 700
EELSITRDCE LYRALNMHYN KANDFEVPER FLEVAQITLR EFFNAIIAGK
710 720 730
DVDPSWKKAI YKVICKLDSE VPEIFKSPNC LQELLHE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JU29 | C9JU29_HUMAN | Prospero homeobox protein 1 | PROX1 | 230 | Annotation score: | ||
U3KPY6 | U3KPY6_HUMAN | Prospero homeobox protein 1 | PROX1 | 143 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 251 – 253 | RQL → LHV in AAC50656 (PubMed:8812486).Curated | 3 | |
Sequence conflicti | 455 – 457 | PAA → LV in AAC50656 (PubMed:8812486).Curated | 3 | |
Sequence conflicti | 724 | I → F in AAC50656 (PubMed:8812486).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049362 | 584 | H → R. Corresponds to variant dbSNP:rs12121210Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U44060 mRNA Translation: AAC50656.1 AK290176 mRNA Translation: BAF82865.1 AC011700 Genomic DNA No translation available. AL606537 Genomic DNA No translation available. CH471100 Genomic DNA Translation: EAW93360.1 BC024201 mRNA Translation: AAH24201.1 |
CCDSi | CCDS31021.1 |
RefSeqi | NP_001257545.1, NM_001270616.1 NP_002754.2, NM_002763.4 XP_016857322.1, XM_017001833.1 |
Genome annotation databases
Ensembli | ENST00000261454; ENSP00000261454; ENSG00000117707 ENST00000366958; ENSP00000355925; ENSG00000117707 ENST00000435016; ENSP00000400694; ENSG00000117707 ENST00000498508; ENSP00000420283; ENSG00000117707 |
GeneIDi | 5629 |
KEGGi | hsa:5629 |
UCSCi | uc001hkg.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U44060 mRNA Translation: AAC50656.1 AK290176 mRNA Translation: BAF82865.1 AC011700 Genomic DNA No translation available. AL606537 Genomic DNA No translation available. CH471100 Genomic DNA Translation: EAW93360.1 BC024201 mRNA Translation: AAH24201.1 |
CCDSi | CCDS31021.1 |
RefSeqi | NP_001257545.1, NM_001270616.1 NP_002754.2, NM_002763.4 XP_016857322.1, XM_017001833.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LMD | NMR | - | A | 575-737 | [»] | |
BMRBi | Q92786 | |||||
SMRi | Q92786 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111613, 20 interactors |
IntActi | Q92786, 12 interactors |
MINTi | Q92786 |
STRINGi | 9606.ENSP00000355925 |
PTM databases
iPTMneti | Q92786 |
PhosphoSitePlusi | Q92786 |
Genetic variation databases
BioMutai | PROX1 |
DMDMi | 85702224 |
Proteomic databases
jPOSTi | Q92786 |
MassIVEi | Q92786 |
MaxQBi | Q92786 |
PaxDbi | Q92786 |
PeptideAtlasi | Q92786 |
PRIDEi | Q92786 |
ProteomicsDBi | 75470 |
Protocols and materials databases
Antibodypediai | 34616, 617 antibodies |
DNASUi | 5629 |
Genome annotation databases
Ensembli | ENST00000261454; ENSP00000261454; ENSG00000117707 ENST00000366958; ENSP00000355925; ENSG00000117707 ENST00000435016; ENSP00000400694; ENSG00000117707 ENST00000498508; ENSP00000420283; ENSG00000117707 |
GeneIDi | 5629 |
KEGGi | hsa:5629 |
UCSCi | uc001hkg.3, human |
Organism-specific databases
CTDi | 5629 |
DisGeNETi | 5629 |
GeneCardsi | PROX1 |
HGNCi | HGNC:9459, PROX1 |
HPAi | ENSG00000117707, Tissue enriched (liver) |
MIMi | 601546, gene |
neXtProti | NX_Q92786 |
OpenTargetsi | ENSG00000117707 |
PharmGKBi | PA33812 |
VEuPathDBi | HostDB:ENSG00000117707.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3779, Eukaryota |
GeneTreei | ENSGT00940000154790 |
HOGENOMi | CLU_016051_0_0_1 |
InParanoidi | Q92786 |
OMAi | QEDVCSN |
OrthoDBi | 373478at2759 |
PhylomeDBi | Q92786 |
TreeFami | TF316638 |
Enzyme and pathway databases
PathwayCommonsi | Q92786 |
SIGNORi | Q92786 |
Miscellaneous databases
BioGRID-ORCSi | 5629, 7 hits in 897 CRISPR screens |
ChiTaRSi | PROX1, human |
GeneWikii | PROX1 |
GenomeRNAii | 5629 |
Pharosi | Q92786, Tbio |
PROi | PR:Q92786 |
RNActi | Q92786, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000117707, Expressed in liver and 186 other tissues |
ExpressionAtlasi | Q92786, baseline and differential |
Genevisiblei | Q92786, HS |
Family and domain databases
Gene3Di | 1.10.10.500, 1 hit |
InterProi | View protein in InterPro IPR023082, Homeo_prospero_dom IPR037131, Homeo_prospero_dom_sf IPR009057, Homeobox-like_sf IPR039350, Prospero_homeodomain IPR007738, Prox1 |
PANTHERi | PTHR12198, PTHR12198, 1 hit PTHR12198:SF6, PTHR12198:SF6, 1 hit |
Pfami | View protein in Pfam PF05044, HPD, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS51818, HOMEO_PROSPERO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PROX1_HUMAN | |
Accessioni | Q92786Primary (citable) accession number: Q92786 Secondary accession number(s): A6NK29 Q8TB91 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | September 13, 2005 | |
Last modified: | February 10, 2021 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants