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Protein

Zinc finger protein ubi-d4

Gene

DPF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed:28533407, PubMed:27775714). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed:28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed:20460684).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri209 – 232C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri270 – 330PHD-type 1PROSITE-ProRule annotationAdd BLAST61
Zinc fingeri327 – 377PHD-type 2PROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processApoptosis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

SIGNORiQ92785

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ubi-d4
Alternative name(s):
Apoptosis response zinc finger protein
BRG1-associated factor 45D
Short name:
BAF45D
D4, zinc and double PHD fingers family 2
Protein requiem
Gene namesi
Name:DPF2
Synonyms:BAF45D, REQ, UBID4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000133884.9
HGNCiHGNC:9964 DPF2
MIMi601671 gene
neXtProtiNX_Q92785

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Coffin-Siris syndrome 7 (CSS7)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of Coffin-Siris syndrome, a congenital multiple malformation syndrome with broad phenotypic and genetic variability. Cardinal features are intellectual disability, coarse facial features, hypertrichosis, and hypoplastic or absent fifth digit nails or phalanges. Additional features include malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems. Sucking/feeding difficulties, poor growth, ophthalmologic abnormalities, hearing impairment, and spinal anomalies are common findings. Both autosomal dominant and autosomal recessive inheritance patterns have been reported. CSS7 inheritance is autosomal dominant.
See also OMIM:618027
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_081047276C → F in CSS7; abolishes interaction with acetylated or methylated histone H3. 1 Publication1
Natural variantiVAR_081048330C → W in CSS7; abolishes interaction with acetylated or methylated histone H3. 1 Publication1
Natural variantiVAR_081049346D → G in CSS7. 1 Publication1
Natural variantiVAR_081050350R → H in CSS7; abolishes interaction with acetylated histone H3; strongly decreased interaction with methylated histone H3. 1 Publication1
Natural variantiVAR_081051369W → R in CSS7. 1 Publication1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi275F → A: Strongly decreased interaction with histones H3 and H4 and loss of function; when associated with A-300 and A-346. 1 Publication1
Mutagenesisi300R → A: Strongly decreased interaction with histones H3 and H4 and loss of function; when associated with A-275 and A-346. 1 Publication1
Mutagenesisi346D → A: Strongly decreased interaction with histones H3 and H4 and loss of function; when associated with A-275 and A-300. 1 Publication1

Keywords - Diseasei

Disease mutation, Mental retardation

Organism-specific databases

MIMi618027 phenotype
OpenTargetsiENSG00000133884
Orphaneti1465 Coffin-Siris syndrome
PharmGKBiPA34331

Polymorphism and mutation databases

BioMutaiDPF2
DMDMi2842711

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001681492 – 391Zinc finger protein ubi-d4Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Cross-linki10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki107Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei142PhosphoserineCombined sources1
Modified residuei172PhosphotyrosineCombined sources1
Modified residuei176PhosphothreonineCombined sources1
Cross-linki178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki196Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei200PhosphoserineCombined sources1
Modified residuei244PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Cross-linki281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ92785
MaxQBiQ92785
PaxDbiQ92785
PeptideAtlasiQ92785
PRIDEiQ92785
ProteomicsDBi75469

PTM databases

iPTMnetiQ92785
PhosphoSitePlusiQ92785

Miscellaneous databases

PMAP-CutDBiQ92785

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000133884 Expressed in 225 organ(s), highest expression level in oocyte
CleanExiHS_DPF2
ExpressionAtlasiQ92785 baseline and differential
GenevisibleiQ92785 HS

Organism-specific databases

HPAiHPA020880
HPA056786

Interactioni

Subunit structurei

Interacts with the nucleosomes, in particular nucleosomes bearing histone H3 crotonylated at 'Lys-14' (H3K14cr) for which DPF2 has high affinity (PubMed:27775714). Also interacts (via PHD-type zinc finger domains) with histone H3 butyrylated at 'Lys-14' (H3K14bu), histone H3 propionylated at 'Lys-14' (H3K14pr), and histone H3 acetylated at 'Lys-14' (H3K14ac) (PubMed:29429572, PubMed:28533407, PubMed:27775714). Interacts with histone H3 acetylated at 'Lys-9' (H3K9ac), histone H3 di-methylated at 'Lys-9' (H3K9me2), and histone H3 tri-methylated at 'Lys-9' (H3K9me3) (PubMed:29429572). Interacts with histone H4 acetylated at 'Lys-12' (H4K12ac) (PubMed:29429572). Interacts with histone H4 acetylated at 'Lys-16' (H4K16ac) (PubMed:28533407). Interacts with SWI/SNF complex components (PubMed:20460684, PubMed:28533407). Interacts with SMARCA2, SMARCA4, SMARCB1 and SMARCD1 (PubMed:20460684). Interacts with SMARCC1, SMARCC2 and ACTL6A (PubMed:28533407). Interacts with RUNX1 (PubMed:28533407).3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111909, 58 interactors
ComplexPortaliCPX-1195 Embryonic stem cell-specific SWI/SNF ATP-dependent chromatin remodeling complex
CORUMiQ92785
DIPiDIP-27575N
IntActiQ92785, 39 interactors
MINTiQ92785
STRINGi9606.ENSP00000436901

Structurei

Secondary structure

1391
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92785
SMRiQ92785
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92785

Family & Domainsi

Sequence similaritiesi

Belongs to the requiem/DPF family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri209 – 232C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri270 – 330PHD-type 1PROSITE-ProRule annotationAdd BLAST61
Zinc fingeri327 – 377PHD-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1244 Eukaryota
ENOG410YDAG LUCA
GeneTreeiENSGT00930000150839
HOGENOMiHOG000217918
HOVERGENiHBG004475
InParanoidiQ92785
KOiK13196
PhylomeDBiQ92785
TreeFamiTF318971

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR025750 Requiem/DPF_N_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF14051 Requiem_N, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SM00355 ZnF_C2H2, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q92785-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ
60 70 80 90 100
SNCYIWMEKR HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP
110 120 130 140 150
DTDQTLKKEG LISQDGSSLE ALLRTDPLEK RGAPDPRVDD DSLGEFPVTN
160 170 180 190 200
SRARKRILEP DDFLDDLDDE DYEEDTPKRR GKGKSKGKGV GSARKKLDAS
210 220 230 240 250
ILEDRDKPYA CDICGKRYKN RPGLSYHYAH SHLAEEEGED KEDSQPPTPV
260 270 280 290 300
SQRSEEQKSK KGPDGLALPN NYCDFCLGDS KINKKTGQPE ELVSCSDCGR
310 320 330 340 350
SGHPSCLQFT PVMMAAVKTY RWQCIECKCC NICGTSENDD QLLFCDDCDR
360 370 380 390
GYHMYCLTPS MSEPPEGSWS CHLCLDLLKE KASIYQNQNS S
Length:391
Mass (Da):44,155
Last modified:November 1, 1997 - v2
Checksum:i1044B4D3036075FC
GO
Isoform 2 (identifier: Q92785-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     156-339: Missing.

Note: No experimental confirmation available.
Show »
Length:207
Mass (Da):23,599
Checksum:i1E065955AD9B3555
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KMZ8J3KMZ8_HUMAN
Zinc finger protein ubi-d4
DPF2
405Annotation score:
H0YEI1H0YEI1_HUMAN
Zinc finger protein ubi-d4
DPF2
116Annotation score:
E9PN04E9PN04_HUMAN
Zinc finger protein ubi-d4
DPF2
106Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_081047276C → F in CSS7; abolishes interaction with acetylated or methylated histone H3. 1 Publication1
Natural variantiVAR_081048330C → W in CSS7; abolishes interaction with acetylated or methylated histone H3. 1 Publication1
Natural variantiVAR_081049346D → G in CSS7. 1 Publication1
Natural variantiVAR_081050350R → H in CSS7; abolishes interaction with acetylated histone H3; strongly decreased interaction with methylated histone H3. 1 Publication1
Natural variantiVAR_081051369W → R in CSS7. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055860156 – 339Missing in isoform 2. 1 PublicationAdd BLAST184

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001433 mRNA Translation: AAB81203.1
U94585 mRNA Translation: AAB58307.1
BT006718 mRNA Translation: AAP35364.1
AY220877 Genomic DNA Translation: AAO26041.1
AK291944 mRNA Translation: BAF84633.1
AK300061 mRNA Translation: BAG61870.1
AP000944 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW74375.1
BC014889 mRNA Translation: AAH14889.1
U43920 Genomic DNA Translation: AAC50687.1
CCDSiCCDS8100.1 [Q92785-1]
RefSeqiNP_006259.1, NM_006268.4 [Q92785-1]
UniGeneiHs.13495
Hs.633609

Genome annotation databases

EnsembliENST00000415073; ENSP00000399714; ENSG00000133884 [Q92785-2]
ENST00000528416; ENSP00000436901; ENSG00000133884 [Q92785-1]
GeneIDi5977
KEGGihsa:5977
UCSCiuc001odm.4 human [Q92785-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001433 mRNA Translation: AAB81203.1
U94585 mRNA Translation: AAB58307.1
BT006718 mRNA Translation: AAP35364.1
AY220877 Genomic DNA Translation: AAO26041.1
AK291944 mRNA Translation: BAF84633.1
AK300061 mRNA Translation: BAG61870.1
AP000944 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW74375.1
BC014889 mRNA Translation: AAH14889.1
U43920 Genomic DNA Translation: AAC50687.1
CCDSiCCDS8100.1 [Q92785-1]
RefSeqiNP_006259.1, NM_006268.4 [Q92785-1]
UniGeneiHs.13495
Hs.633609

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IUFX-ray1.80A203-249[»]
5B79X-ray2.60A270-391[»]
5VDCX-ray1.60A270-391[»]
ProteinModelPortaliQ92785
SMRiQ92785
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111909, 58 interactors
ComplexPortaliCPX-1195 Embryonic stem cell-specific SWI/SNF ATP-dependent chromatin remodeling complex
CORUMiQ92785
DIPiDIP-27575N
IntActiQ92785, 39 interactors
MINTiQ92785
STRINGi9606.ENSP00000436901

PTM databases

iPTMnetiQ92785
PhosphoSitePlusiQ92785

Polymorphism and mutation databases

BioMutaiDPF2
DMDMi2842711

Proteomic databases

EPDiQ92785
MaxQBiQ92785
PaxDbiQ92785
PeptideAtlasiQ92785
PRIDEiQ92785
ProteomicsDBi75469

Protocols and materials databases

DNASUi5977
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000415073; ENSP00000399714; ENSG00000133884 [Q92785-2]
ENST00000528416; ENSP00000436901; ENSG00000133884 [Q92785-1]
GeneIDi5977
KEGGihsa:5977
UCSCiuc001odm.4 human [Q92785-1]

Organism-specific databases

CTDi5977
EuPathDBiHostDB:ENSG00000133884.9
GeneCardsiDPF2
HGNCiHGNC:9964 DPF2
HPAiHPA020880
HPA056786
MIMi601671 gene
618027 phenotype
neXtProtiNX_Q92785
OpenTargetsiENSG00000133884
Orphaneti1465 Coffin-Siris syndrome
PharmGKBiPA34331
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1244 Eukaryota
ENOG410YDAG LUCA
GeneTreeiENSGT00930000150839
HOGENOMiHOG000217918
HOVERGENiHBG004475
InParanoidiQ92785
KOiK13196
PhylomeDBiQ92785
TreeFamiTF318971

Enzyme and pathway databases

SIGNORiQ92785

Miscellaneous databases

ChiTaRSiDPF2 human
EvolutionaryTraceiQ92785
GeneWikiiDPF2
GenomeRNAii5977
PMAP-CutDBiQ92785
PROiPR:Q92785
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133884 Expressed in 225 organ(s), highest expression level in oocyte
CleanExiHS_DPF2
ExpressionAtlasiQ92785 baseline and differential
GenevisibleiQ92785 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR025750 Requiem/DPF_N_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF14051 Requiem_N, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SM00355 ZnF_C2H2, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiREQU_HUMAN
AccessioniPrimary (citable) accession number: Q92785
Secondary accession number(s): A8K7C9, B4DT58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 186 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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