Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription initiation factor TFIID subunit 4B

Gene

TAF4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.1 PublicationBy similarity3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • DNA-binding transcription factor activity Source: InterPro
  • NF-kappaB binding Source: UniProtKB
  • protein heterodimerization activity Source: InterPro
  • transcription factor binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDifferentiation, Oogenesis, Spermatogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 4B
Alternative name(s):
Transcription initiation factor TFIID 105 kDa subunit
Short name:
TAF(II)105
Short name:
TAFII-105
Short name:
TAFII105
Gene namesi
Name:TAF4B
Synonyms:TAF2C2, TAFII105
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000141384.11
HGNCiHGNC:11538 TAF4B
MIMi601689 gene
neXtProtiNX_Q92750

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Spermatogenic failure 13 (SPGF13)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility.
See also OMIM:615841

Organism-specific databases

DisGeNETi6875
MalaCardsiTAF4B
MIMi615841 phenotype
OpenTargetsiENSG00000141384
Orphaneti399805 Male infertility with azoospermia or oligozoospermia due to single gene mutation
PharmGKBiPA36313

Polymorphism and mutation databases

BioMutaiTAF4B
DMDMi119370533

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188721 – 862Transcription initiation factor TFIID subunit 4BAdd BLAST862

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei595PhosphoserineCombined sources1

Post-translational modificationi

Under stimulation by forskolin, Isoform 1 is phosphorylated by protein kinase A (PKA).1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ92750
MaxQBiQ92750
PaxDbiQ92750
PeptideAtlasiQ92750
PRIDEiQ92750
ProteomicsDBi75440
75441 [Q92750-2]

PTM databases

iPTMnetiQ92750
PhosphoSitePlusiQ92750

Expressioni

Tissue specificityi

Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells.2 Publications

Gene expression databases

BgeeiENSG00000141384 Expressed in 186 organ(s), highest expression level in female gonad
CleanExiHS_TAF4B
ExpressionAtlasiQ92750 baseline and differential
GenevisibleiQ92750 HS

Organism-specific databases

HPAiHPA028937

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Heterodimerizes with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain. This heterodimer forms a histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer. Interacts with P65/RELA homodimers and P65/RELA-REL heterodimers. Interaction with POU2AF1, via its C-terminal activation domain, is required for octamer-dependent transcription.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112738, 14 interactors
ComplexPortaliCPX-930 General transcription factor complex TFIID, TAF4B variant
CORUMiQ92750
IntActiQ92750, 5 interactors
STRINGi9606.ENSP00000269142

Structurei

3D structure databases

ProteinModelPortaliQ92750
SMRiQ92750
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 353TAFHPROSITE-ProRule annotationAdd BLAST98
Domaini653 – 702Histone-foldAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni511 – 533Required for interaction with P65/RELAAdd BLAST23
Regioni830 – 862Required for interaction with TAF121 PublicationAdd BLAST33

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili722 – 787Sequence analysisAdd BLAST66

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi516 – 556Nuclear export signalAdd BLAST41

Sequence similaritiesi

Belongs to the TAF4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2341 Eukaryota
ENOG410XQS6 LUCA
GeneTreeiENSGT00390000011620
HOGENOMiHOG000154500
HOVERGENiHBG103779
InParanoidiQ92750
KOiK03129
PhylomeDBiQ92750
TreeFamiTF316520

Family and domain databases

CDDicd08045 TAF4, 1 hit
Gene3Di1.10.20.10, 1 hit
1.20.120.1110, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007900 TAF4
IPR037249 TAFH/NHR1_dom_sf
IPR003894 TAFH_NHR1
PfamiView protein in Pfam
PF05236 TAF4, 1 hit
PF07531 TAFH, 1 hit
SMARTiView protein in SMART
SM00549 TAFH, 1 hit
SUPFAMiSSF158553 SSF158553, 1 hit
SSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS51119 TAFH, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q92750-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPAGLTEPAG AAPPAAVSAS GTVTMAPAGA LPVRVESTPV ALGAVTKAPV
60 70 80 90 100
SVCVEPTASQ PLRSPVGTLV TKVAPVSAPP KVSSGPRLPA PQIVAVKAPN
110 120 130 140 150
TTTIQFPANL QLPPGTVLIK SNSGPLMLVS PQQTVTRAET TSNITSRPAV
160 170 180 190 200
PANPQTVKIC TVPNSSSQLI KKVAVTPVKK LAQIGTTVVT TVPKPSSVQS
210 220 230 240 250
VAVPTSVVTV TPGKPLNTVT TLKPSSLGAS STPSNEPNLK AENSAAVQIN
260 270 280 290 300
LSPTMLENVK KCKNFLAMLI KLACSGSQSP EMGQNVKKLV EQLLDAKIEA
310 320 330 340 350
EEFTRKLYVE LKSSPQPHLV PFLKKSVVAL RQLLPNSQSF IQQCVQQTSS
360 370 380 390 400
DMVIATCTTT VTTSPVVTTT VSSSQSEKSI IVSGATAPRT VSVQTLNPLA
410 420 430 440 450
GPVGAKAGVV TLHSVGPTAA TGGTTAGTGL LQTSKPLVTS VANTVTTVSL
460 470 480 490 500
QPEKPVVSGT AVTLSLPAVT FGETSGAAIC LPSVKPVVSS AGTTSDKPVI
510 520 530 540 550
GTPVQIKLAQ PGPVLSQPAG IPQAVQVKQL VVQQPSGGNE KQVTTISHSS
560 570 580 590 600
TLTIQKCGQK TMPVNTIIPT SQFPPASILK QITLPGNKIL SLQASPTQKN
610 620 630 640 650
RIKENVTSCF RDEDDINDVT SMAGVNLNEE NACILATNSE LVGTLIQSCK
660 670 680 690 700
DEPFLFIGAL QKRILDIGKK HDITELNSDA VNLISQATQE RLRGLLEKLT
710 720 730 740 750
AIAQHRMTTY KASENYILCS DTRSQLKFLE KLDQLEKQRK DLEEREMLLK
760 770 780 790 800
AAKSRSNKED PEQLRLKQKA KELQQLELAQ IQHRDANLTA LAAIGPRKKR
810 820 830 840 850
PLESGIEGLK DNLLASGTSS LTATKQLHRP RITRICLRDL IFCMEQEREM
860
KYSRALYLAL LK
Length:862
Mass (Da):91,091
Last modified:December 12, 2006 - v2
Checksum:iE380D5CD2445AC11
GO
Isoform 2 (identifier: Q92750-2) [UniParc]FASTAAdd to basket
Also known as: TAFII105-E9

The sequence of this isoform differs from the canonical sequence as follows:
     612-618: DEDDIND → STFICVY
     619-862: Missing.

Note: May be due to exon inclusion. No experimental confirmation available. Unphosphorylated form.
Show »
Length:618
Mass (Da):63,364
Checksum:i0EA429488262537F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KTH2J3KTH2_HUMAN
HCG38478, isoform CRA_a
TAF4B hCG_38478
867Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti490 – 496SAGTTSD → FCWDHIC in CAA70499 (PubMed:8858156).Curated7
Sequence conflicti523 – 530QAVQVKQL → TGSSSKQLFSLFH in CAA70499 (PubMed:8858156).Curated8

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052259249I → V. Corresponds to variant dbSNP:rs16942219Ensembl.1
Natural variantiVAR_061836539N → S. Corresponds to variant dbSNP:rs12963653Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022025612 – 618DEDDIND → STFICVY in isoform 2. 1 Publication7
Alternative sequenceiVSP_022026619 – 862Missing in isoform 2. 1 PublicationAdd BLAST244

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY888048 mRNA Translation: AAX14897.1
AY888049 mRNA Translation: AAX14898.1
Y09321 mRNA Translation: CAA70499.1
CCDSiCCDS42421.1 [Q92750-1]
RefSeqiNP_001280654.1, NM_001293725.1
NP_005631.1, NM_005640.2 [Q92750-1]
UniGeneiHs.369519

Genome annotation databases

EnsembliENST00000269142; ENSP00000269142; ENSG00000141384 [Q92750-1]
ENST00000418698; ENSP00000389365; ENSG00000141384 [Q92750-2]
GeneIDi6875
KEGGihsa:6875
UCSCiuc002kvu.5 human [Q92750-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY888048 mRNA Translation: AAX14897.1
AY888049 mRNA Translation: AAX14898.1
Y09321 mRNA Translation: CAA70499.1
CCDSiCCDS42421.1 [Q92750-1]
RefSeqiNP_001280654.1, NM_001293725.1
NP_005631.1, NM_005640.2 [Q92750-1]
UniGeneiHs.369519

3D structure databases

ProteinModelPortaliQ92750
SMRiQ92750
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112738, 14 interactors
ComplexPortaliCPX-930 General transcription factor complex TFIID, TAF4B variant
CORUMiQ92750
IntActiQ92750, 5 interactors
STRINGi9606.ENSP00000269142

PTM databases

iPTMnetiQ92750
PhosphoSitePlusiQ92750

Polymorphism and mutation databases

BioMutaiTAF4B
DMDMi119370533

Proteomic databases

EPDiQ92750
MaxQBiQ92750
PaxDbiQ92750
PeptideAtlasiQ92750
PRIDEiQ92750
ProteomicsDBi75440
75441 [Q92750-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000269142; ENSP00000269142; ENSG00000141384 [Q92750-1]
ENST00000418698; ENSP00000389365; ENSG00000141384 [Q92750-2]
GeneIDi6875
KEGGihsa:6875
UCSCiuc002kvu.5 human [Q92750-1]

Organism-specific databases

CTDi6875
DisGeNETi6875
EuPathDBiHostDB:ENSG00000141384.11
GeneCardsiTAF4B
HGNCiHGNC:11538 TAF4B
HPAiHPA028937
MalaCardsiTAF4B
MIMi601689 gene
615841 phenotype
neXtProtiNX_Q92750
OpenTargetsiENSG00000141384
Orphaneti399805 Male infertility with azoospermia or oligozoospermia due to single gene mutation
PharmGKBiPA36313
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2341 Eukaryota
ENOG410XQS6 LUCA
GeneTreeiENSGT00390000011620
HOGENOMiHOG000154500
HOVERGENiHBG103779
InParanoidiQ92750
KOiK03129
PhylomeDBiQ92750
TreeFamiTF316520

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Miscellaneous databases

ChiTaRSiTAF4B human
GeneWikiiTAF4B
GenomeRNAii6875
PROiPR:Q92750
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141384 Expressed in 186 organ(s), highest expression level in female gonad
CleanExiHS_TAF4B
ExpressionAtlasiQ92750 baseline and differential
GenevisibleiQ92750 HS

Family and domain databases

CDDicd08045 TAF4, 1 hit
Gene3Di1.10.20.10, 1 hit
1.20.120.1110, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007900 TAF4
IPR037249 TAFH/NHR1_dom_sf
IPR003894 TAFH_NHR1
PfamiView protein in Pfam
PF05236 TAF4, 1 hit
PF07531 TAFH, 1 hit
SMARTiView protein in SMART
SM00549 TAFH, 1 hit
SUPFAMiSSF158553 SSF158553, 1 hit
SSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS51119 TAFH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTAF4B_HUMAN
AccessioniPrimary (citable) accession number: Q92750
Secondary accession number(s): Q29YA4, Q29YA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 12, 2006
Last modified: November 7, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again