UniProtKB - Q92643 (GPI8_HUMAN)
Protein
GPI-anchor transamidase
Gene
PIGK
Organism
Homo sapiens (Human)
Status
Functioni
Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.
: glycosylphosphatidylinositol-anchor biosynthesis Pathwayi
This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 164 | 1 Publication | 1 | |
Active sitei | 206 | 1 Publication | 1 |
GO - Molecular functioni
- GPI-anchor transamidase activity Source: UniProtKB
- protein disulfide isomerase activity Source: UniProtKB
GO - Biological processi
- attachment of GPI anchor to protein Source: GO_Central
- protein localization to cell surface Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | GPI-anchor biosynthesis |
Enzyme and pathway databases
PathwayCommonsi | Q92643 |
Reactomei | R-HSA-162791, Attachment of GPI anchor to uPAR |
SIGNORi | Q92643 |
UniPathwayi | UPA00196 |
Protein family/group databases
MEROPSi | C13.005 |
Names & Taxonomyi
Protein namesi | Recommended name: GPI-anchor transamidase (EC:3.-.-.-)Short name: GPI transamidase Alternative name(s): GPI8 homolog Short name: hGPI8 Phosphatidylinositol-glycan biosynthesis class K protein Short name: PIG-K |
Gene namesi | Name:PIGK Synonyms:GPI8 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8965, PIGK |
MIMi | 605087, gene |
neXtProti | NX_Q92643 |
VEuPathDBi | HostDB:ENSG00000142892.14 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
- GPI-anchor transamidase complex Source: UniProtKB
Other locations
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 367 | LumenalSequence analysisAdd BLAST | 340 | |
Transmembranei | 368 – 388 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 389 – 395 | CytoplasmicSequence analysis | 7 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 92 | C → A: Partial loss of activity. 2 Publications | 1 | |
Mutagenesisi | 92 | C → S: Decrease in activity. 2 Publications | 1 | |
Mutagenesisi | 164 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 206 | C → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 311 – 395 | Missing : Loss of activity. 1 PublicationAdd BLAST | 85 |
Organism-specific databases
DisGeNETi | 10026 |
MalaCardsi | PIGK |
OpenTargetsi | ENSG00000142892 |
PharmGKBi | PA33296 |
Miscellaneous databases
Pharosi | Q92643, Tbio |
Genetic variation databases
BioMutai | PIGK |
DMDMi | 22001630 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Combined sources1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000026529 | 28 – 395 | GPI-anchor transamidaseAdd BLAST | 368 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 92 | Interchain (with C-182 in PIGT)1 Publication |
Post-translational modificationi
The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | Q92643 |
jPOSTi | Q92643 |
MassIVEi | Q92643 |
MaxQBi | Q92643 |
PaxDbi | Q92643 |
PeptideAtlasi | Q92643 |
PRIDEi | Q92643 |
ProteomicsDBi | 5832 75395 [Q92643-1] |
PTM databases
iPTMneti | Q92643 |
PhosphoSitePlusi | Q92643 |
Expressioni
Gene expression databases
Bgeei | ENSG00000142892, Expressed in corpus callosum and 231 other tissues |
ExpressionAtlasi | Q92643, baseline and differential |
Genevisiblei | Q92643, HS |
Organism-specific databases
HPAi | ENSG00000142892, Low tissue specificity |
Interactioni
Subunit structurei
Forms a complex with PIGT, PIGS, PIGU and GAA1.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 115343, 28 interactors |
CORUMi | Q92643 |
IntActi | Q92643, 15 interactors |
MINTi | Q92643 |
STRINGi | 9606.ENSP00000359848 |
Miscellaneous databases
RNActi | Q92643, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 311 – 321 | Essential for GPI attachmentAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the peptidase C13 family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1349, Eukaryota |
GeneTreei | ENSGT00940000156273 |
InParanoidi | Q92643 |
OMAi | VMESQFP |
OrthoDBi | 1259741at2759 |
PhylomeDBi | Q92643 |
TreeFami | TF300848 |
Family and domain databases
InterProi | View protein in InterPro IPR028361, GPI_transamidase IPR001096, Peptidase_C13 |
PANTHERi | PTHR48067, PTHR48067, 1 hit |
Pfami | View protein in Pfam PF01650, Peptidase_C13, 1 hit |
PIRSFi | PIRSF500138, GPI8, 1 hit PIRSF019663, Legumain, 1 hit |
PRINTSi | PR00776, HEMOGLOBNASE |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q92643-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVTDSLSRA ATVLATVLLL SFGSVAASHI EDQAEQFFRS GHTNNWAVLV
60 70 80 90 100
CTSRFWFNYR HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNPRNPKPA
110 120 130 140 150
TVFSHKNMEL NVYGDDVEVD YRSYEVTVEN FLRVLTGRIP PSTPRSKRLL
160 170 180 190 200
SDDRSNILIY MTGHGGNGFL KFQDSEEITN IELADAFEQM WQKRRYNELL
210 220 230 240 250
FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA IGVHLMDRYT
260 270 280 290 300
FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FQRDPKNVLI
310 320 330 340 350
TDFFGSVRKV EITTETIKLQ QDSEIMESSY KEDQMDEKLM EPLKYAEQLP
360 370 380 390
VAQIIHQKPK LKDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AK81 | B1AK81_HUMAN | GPI-anchor transamidase | PIGK hCG_22810 | 301 | Annotation score: | ||
A6NEM5 | A6NEM5_HUMAN | GPI-anchor transamidase | PIGK | 332 | Annotation score: | ||
S4R3M5 | S4R3M5_HUMAN | GPI-anchor transamidase | PIGK | 27 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 4 | MAVT → SLHEA (PubMed:8978684).Curated | 4 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051518 | 16 | T → A. Corresponds to variant dbSNP:rs12723684Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056457 | 50 – 125 | Missing in isoform 2. 1 PublicationAdd BLAST | 76 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07596 mRNA Translation: CAA68871.1 AF022913 mRNA Translation: AAB81597.1 AK304340 mRNA Translation: BAG65185.1 AK313015 mRNA Translation: BAG35850.1 AC093433 Genomic DNA No translation available. AC113935 Genomic DNA No translation available. AL035409 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06380.1 BC020737 mRNA Translation: AAH20737.1 |
CCDSi | CCDS674.1 [Q92643-1] |
RefSeqi | NP_005473.1, NM_005482.2 [Q92643-1] |
Genome annotation databases
Ensembli | ENST00000370812; ENSP00000359848; ENSG00000142892 [Q92643-1] |
GeneIDi | 10026 |
KEGGi | hsa:10026 |
UCSCi | uc001dhk.4, human [Q92643-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07596 mRNA Translation: CAA68871.1 AF022913 mRNA Translation: AAB81597.1 AK304340 mRNA Translation: BAG65185.1 AK313015 mRNA Translation: BAG35850.1 AC093433 Genomic DNA No translation available. AC113935 Genomic DNA No translation available. AL035409 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06380.1 BC020737 mRNA Translation: AAH20737.1 |
CCDSi | CCDS674.1 [Q92643-1] |
RefSeqi | NP_005473.1, NM_005482.2 [Q92643-1] |
3D structure databases
SMRi | Q92643 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115343, 28 interactors |
CORUMi | Q92643 |
IntActi | Q92643, 15 interactors |
MINTi | Q92643 |
STRINGi | 9606.ENSP00000359848 |
Protein family/group databases
MEROPSi | C13.005 |
PTM databases
iPTMneti | Q92643 |
PhosphoSitePlusi | Q92643 |
Genetic variation databases
BioMutai | PIGK |
DMDMi | 22001630 |
Proteomic databases
EPDi | Q92643 |
jPOSTi | Q92643 |
MassIVEi | Q92643 |
MaxQBi | Q92643 |
PaxDbi | Q92643 |
PeptideAtlasi | Q92643 |
PRIDEi | Q92643 |
ProteomicsDBi | 5832 75395 [Q92643-1] |
Protocols and materials databases
Antibodypediai | 33483, 172 antibodies |
DNASUi | 10026 |
Genome annotation databases
Ensembli | ENST00000370812; ENSP00000359848; ENSG00000142892 [Q92643-1] |
GeneIDi | 10026 |
KEGGi | hsa:10026 |
UCSCi | uc001dhk.4, human [Q92643-1] |
Organism-specific databases
CTDi | 10026 |
DisGeNETi | 10026 |
GeneCardsi | PIGK |
HGNCi | HGNC:8965, PIGK |
HPAi | ENSG00000142892, Low tissue specificity |
MalaCardsi | PIGK |
MIMi | 605087, gene |
neXtProti | NX_Q92643 |
OpenTargetsi | ENSG00000142892 |
PharmGKBi | PA33296 |
VEuPathDBi | HostDB:ENSG00000142892.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1349, Eukaryota |
GeneTreei | ENSGT00940000156273 |
InParanoidi | Q92643 |
OMAi | VMESQFP |
OrthoDBi | 1259741at2759 |
PhylomeDBi | Q92643 |
TreeFami | TF300848 |
Enzyme and pathway databases
UniPathwayi | UPA00196 |
PathwayCommonsi | Q92643 |
Reactomei | R-HSA-162791, Attachment of GPI anchor to uPAR |
SIGNORi | Q92643 |
Miscellaneous databases
BioGRID-ORCSi | 10026, 25 hits in 874 CRISPR screens |
ChiTaRSi | PIGK, human |
GeneWikii | PIGK |
GenomeRNAii | 10026 |
Pharosi | Q92643, Tbio |
PROi | PR:Q92643 |
RNActi | Q92643, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000142892, Expressed in corpus callosum and 231 other tissues |
ExpressionAtlasi | Q92643, baseline and differential |
Genevisiblei | Q92643, HS |
Family and domain databases
InterProi | View protein in InterPro IPR028361, GPI_transamidase IPR001096, Peptidase_C13 |
PANTHERi | PTHR48067, PTHR48067, 1 hit |
Pfami | View protein in Pfam PF01650, Peptidase_C13, 1 hit |
PIRSFi | PIRSF500138, GPI8, 1 hit PIRSF019663, Legumain, 1 hit |
PRINTSi | PR00776, HEMOGLOBNASE |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPI8_HUMAN | |
Accessioni | Q92643Primary (citable) accession number: Q92643 Secondary accession number(s): B2R7K3 Q5TG77 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2002 |
Last sequence update: | July 11, 2002 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries