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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

Gene

DHX38

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase involved in pre-mRNA splicing.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi555 – 562ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' RNA helicase activity Source: GO_Central
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase DHX38
DEAH box protein 38
Gene namesi
Name:DHX38
Synonyms:DDX38, KIAA0224, PRP16
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000140829.11
HGNCiHGNC:17211 DHX38
MIMi605584 gene
neXtProtiNX_Q92620

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi9785
OpenTargetsiENSG00000140829
PharmGKBiPA27225

Polymorphism and mutation databases

BioMutaiDHX38
DMDMi85700389

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000551472 – 1227Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16Add BLAST1226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineCombined sources1 Publication1
Modified residuei56PhosphoserineCombined sources1
Modified residuei117PhosphothreonineCombined sources1
Modified residuei199PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei260N6-acetyllysineCombined sources1
Cross-linki482Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki483Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki504Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki1166Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1194PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ92620
MaxQBiQ92620
PaxDbiQ92620
PeptideAtlasiQ92620
PRIDEiQ92620
ProteomicsDBi75373

PTM databases

iPTMnetiQ92620
PhosphoSitePlusiQ92620

Expressioni

Gene expression databases

BgeeiENSG00000140829 Expressed in 200 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_DHX38
ExpressionAtlasiQ92620 baseline and differential
GenevisibleiQ92620 HS

Organism-specific databases

HPAiHPA041347
HPA041604

Interactioni

Subunit structurei

Identified in the spliceosome C complex.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi115129, 58 interactors
CORUMiQ92620
IntActiQ92620, 20 interactors
MINTiQ92620
STRINGi9606.ENSP00000268482

Structurei

3D structure databases

ProteinModelPortaliQ92620
SMRiQ92620
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini542 – 705Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST164
Domaini727 – 902Helicase C-terminalPROSITE-ProRule annotationAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi652 – 655DEAH box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0925 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiQ92620
KOiK12815
OMAiFIHTKAM
OrthoDBiEOG091G030Q
PhylomeDBiQ92620
TreeFamiTF105793

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q92620-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGDTSEDASI HRLEGTDLDC QVGGLICKSK SAASEQHVFK APAPRPSLLG
60 70 80 90 100
LDLLASLKRR EREEKDDGED KKKSKVSSYK DWEESKDDQK DAEEEGGDQA
110 120 130 140 150
GQNIRKDRHY RSARVETPSH PGGVSEEFWE RSRQRERERR EHGVYASSKE
160 170 180 190 200
EKDWKKEKSR DRDYDRKRDR DERDRSRHSS RSERDGGSER SSRRNEPESP
210 220 230 240 250
RHRPKDAATP SRSTWEEEDS GYGSSRRSQW ESPSPTPSYR DSERSHRLST
260 270 280 290 300
RDRDRSVRGK YSDDTPLPTP SYKYNEWADD RRHLGSTPRL SRGRGRREEG
310 320 330 340 350
EEGISFDTEE ERQQWEDDQR QADRDWYMMD EGYDEFHNPL AYSSEDYVRR
360 370 380 390 400
REQHLHKQKQ KRISAQRRQI NEDNERWETN RMLTSGVVHR LEVDEDFEED
410 420 430 440 450
NAAKVHLMVH NLVPPFLDGR IVFTKQPEPV IPVKDATSDL AIIARKGSQT
460 470 480 490 500
VRKHREQKER KKAQHKHWEL AGTKLGDIMG VKKEEEPDKA VTEDGKVDYR
510 520 530 540 550
TEQKFADHMK RKSEASSEFA KKKSILEQRQ YLPIFAVQQE LLTIIRDNSI
560 570 580 590 600
VIVVGETGSG KTTQLTQYLH EDGYTDYGMI GCTQPRRVAA MSVAKRVSEE
610 620 630 640 650
MGGNLGEEVG YAIRFEDCTS ENTLIKYMTD GILLRESLRE ADLDHYSAII
660 670 680 690 700
MDEAHERSLN TDVLFGLLRE VVARRSDLKL IVTSATMDAE KFAAFFGNVP
710 720 730 740 750
IFHIPGRTFP VDILFSKTPQ EDYVEAAVKQ SLQVHLSGAP GDILIFMPGQ
760 770 780 790 800
EDIEVTSDQI VEHLEELENA PALAVLPIYS QLPSDLQAKI FQKAPDGVRK
810 820 830 840 850
CIVATNIAET SLTVDGIMFV IDSGYCKLKV FNPRIGMDAL QIYPISQANA
860 870 880 890 900
NQRSGRAGRT GPGQCFRLYT QSAYKNELLT TTVPEIQRTN LANVVLLLKS
910 920 930 940 950
LGVQDLLQFH FMDPPPEDNM LNSMYQLWIL GALDNTGGLT STGRLMVEFP
960 970 980 990 1000
LDPALSKMLI VSCDMGCSSE ILLIVSMLSV PAIFYRPKGR EEESDQIREK
1010 1020 1030 1040 1050
FAVPESDHLT YLNVYLQWKN NNYSTIWCND HFIHAKAMRK VREVRAQLKD
1060 1070 1080 1090 1100
IMVQQRMSLA SCGTDWDIVR KCICAAYFHQ AAKLKGIGEY VNIRTGMPCH
1110 1120 1130 1140 1150
LHPTSSLFGM GYTPDYIVYH ELVMTTKEYM QCVTAVDGEW LAELGPMFYS
1160 1170 1180 1190 1200
VKQAGKSRQE NRRRAKEEAS AMEEEMALAE EQLRARRQEQ EKRSPLGSVR
1210 1220
STKIYTPGRK EQGEPMTPRR TPARFGL
Length:1,227
Mass (Da):140,503
Last modified:January 24, 2006 - v2
Checksum:i80D06118D0D465E5
GO
Isoform 2 (identifier: Q92620-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     99-786: Missing.

Note: No experimental confirmation available.
Show »
Length:539
Mass (Da):60,743
Checksum:i4BE6EB5D8640A4A9
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BMS7H3BMS7_HUMAN
Pre-mRNA-splicing factor ATP-depend...
DHX38
191Annotation score:
J3KTG2J3KTG2_HUMAN
Pre-mRNA-splicing factor ATP-depend...
DHX38
128Annotation score:
J3KSA8J3KSA8_HUMAN
Pre-mRNA-splicing factor ATP-depend...
DHX38
187Annotation score:
J3KRT1J3KRT1_HUMAN
Pre-mRNA-splicing factor ATP-depend...
DHX38
142Annotation score:
H3BV01H3BV01_HUMAN
Pre-mRNA-splicing factor ATP-depend...
DHX38
123Annotation score:
H3BQT9H3BQT9_HUMAN
Pre-mRNA-splicing factor ATP-depend...
DHX38
138Annotation score:

Sequence cautioni

The sequence BAA13213 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0155181217T → A2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05604599 – 786Missing in isoform 2. 1 PublicationAdd BLAST688

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038391 mRNA Translation: AAC39729.1
D86977 mRNA Translation: BAA13213.2 Different initiation.
AK301074 mRNA Translation: BAG62680.1
AC009087 Genomic DNA No translation available.
AC004682 Genomic DNA Translation: AAC27431.1
CH471166 Genomic DNA Translation: EAW59180.1
CH471166 Genomic DNA Translation: EAW59190.1
BC004235 mRNA Translation: AAH04235.1
BC008340 mRNA Translation: AAH08340.1
CCDSiCCDS10907.1 [Q92620-1]
RefSeqiNP_054722.2, NM_014003.3 [Q92620-1]
XP_011521786.1, XM_011523484.1 [Q92620-1]
XP_011521787.1, XM_011523485.1 [Q92620-1]
UniGeneiHs.151218

Genome annotation databases

EnsembliENST00000268482; ENSP00000268482; ENSG00000140829 [Q92620-1]
GeneIDi9785
KEGGihsa:9785
UCSCiuc002fcb.4 human [Q92620-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038391 mRNA Translation: AAC39729.1
D86977 mRNA Translation: BAA13213.2 Different initiation.
AK301074 mRNA Translation: BAG62680.1
AC009087 Genomic DNA No translation available.
AC004682 Genomic DNA Translation: AAC27431.1
CH471166 Genomic DNA Translation: EAW59180.1
CH471166 Genomic DNA Translation: EAW59190.1
BC004235 mRNA Translation: AAH04235.1
BC008340 mRNA Translation: AAH08340.1
CCDSiCCDS10907.1 [Q92620-1]
RefSeqiNP_054722.2, NM_014003.3 [Q92620-1]
XP_011521786.1, XM_011523484.1 [Q92620-1]
XP_011521787.1, XM_011523485.1 [Q92620-1]
UniGeneiHs.151218

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5YZGelectron microscopy4.10Z1-1227[»]
ProteinModelPortaliQ92620
SMRiQ92620
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115129, 58 interactors
CORUMiQ92620
IntActiQ92620, 20 interactors
MINTiQ92620
STRINGi9606.ENSP00000268482

PTM databases

iPTMnetiQ92620
PhosphoSitePlusiQ92620

Polymorphism and mutation databases

BioMutaiDHX38
DMDMi85700389

Proteomic databases

EPDiQ92620
MaxQBiQ92620
PaxDbiQ92620
PeptideAtlasiQ92620
PRIDEiQ92620
ProteomicsDBi75373

Protocols and materials databases

DNASUi9785
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268482; ENSP00000268482; ENSG00000140829 [Q92620-1]
GeneIDi9785
KEGGihsa:9785
UCSCiuc002fcb.4 human [Q92620-1]

Organism-specific databases

CTDi9785
DisGeNETi9785
EuPathDBiHostDB:ENSG00000140829.11
GeneCardsiDHX38
HGNCiHGNC:17211 DHX38
HPAiHPA041347
HPA041604
MIMi605584 gene
neXtProtiNX_Q92620
OpenTargetsiENSG00000140829
PharmGKBiPA27225
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0925 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiQ92620
KOiK12815
OMAiFIHTKAM
OrthoDBiEOG091G030Q
PhylomeDBiQ92620
TreeFamiTF105793

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing

Miscellaneous databases

ChiTaRSiDHX38 human
GeneWikiiDHX38
GenomeRNAii9785
PROiPR:Q92620
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140829 Expressed in 200 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_DHX38
ExpressionAtlasiQ92620 baseline and differential
GenevisibleiQ92620 HS

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRP16_HUMAN
AccessioniPrimary (citable) accession number: Q92620
Secondary accession number(s): B4DVG8
, D3DWS7, O75212, Q96HN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 24, 2006
Last modified: November 7, 2018
This is version 183 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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