UniProtKB - Q925U4 (EDEM1_MOUSE)
ER degradation-enhancing alpha-mannosidase-like protein 1
Edem1
Functioni
Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.
By similarity1 PublicationGO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: ParkinsonsUK-UCL
- misfolded protein binding Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- mannose trimming involved in glycoprotein ERAD pathway Source: MGI
- positive regulation of retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
- protein targeting to ER Source: MGI
- response to unfolded protein Source: UniProtKB-KW
- trimming of terminal mannose on C branch Source: ParkinsonsUK-UCL
- ubiquitin-dependent ERAD pathway Source: MGI
- ubiquitin-dependent glycoprotein ERAD pathway Source: MGI
Keywordsi
Biological process | Unfolded protein response |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: ER degradation-enhancing alpha-mannosidase-like protein 1 |
Gene namesi | Name:Edem1 Synonyms:Edem |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2180139, Edem1 |
VEuPathDBi | HostDB:ENSMUSG00000030104 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum quality control compartment Source: MGI
- integral component of endoplasmic reticulum membrane Source: MGI
Other locations
- aggresome Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 5 – 25 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 26 – 652 | LumenalSequence analysisAdd BLAST | 627 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210322 | 1 – 652 | ER degradation-enhancing alpha-mannosidase-like protein 1Add BLAST | 652 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 193 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 294 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 337 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q925U4 |
jPOSTi | Q925U4 |
MaxQBi | Q925U4 |
PaxDbi | Q925U4 |
PRIDEi | Q925U4 |
ProteomicsDBi | 277715 |
PTM databases
GlyGeni | Q925U4, 4 sites |
iPTMneti | Q925U4 |
PhosphoSitePlusi | Q925U4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000030104, Expressed in pancreas and 295 other tissues |
ExpressionAtlasi | Q925U4, baseline and differential |
Genevisiblei | Q925U4, MM |
Interactioni
Subunit structurei
Interacts with DERL2 and DERL3. Binds to SEL1L (By similarity).
Interacts with DNAJC10.
By similarity2 PublicationsGO - Molecular functioni
- misfolded protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 228670, 4 interactors |
IntActi | Q925U4, 8 interactors |
STRINGi | 10090.ENSMUSP00000086565 |
Miscellaneous databases
RNActi | Q925U4, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 50 – 86 | DisorderedSequence analysisAdd BLAST | 37 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2429, Eukaryota |
GeneTreei | ENSGT00940000157717 |
HOGENOMi | CLU_003818_5_6_1 |
InParanoidi | Q925U4 |
OMAi | NWTFVKD |
OrthoDBi | 434316at2759 |
PhylomeDBi | Q925U4 |
TreeFami | TF300807 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR044674, EDEM1/2/3 IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
PANTHERi | PTHR45679, PTHR45679, 1 hit |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MQWRALVLGL VLLRLGLHAV LWLVFGLGPS MGFYQRFPLS FGFQRLRDPD
60 70 80 90 100
GSGPVGPPGG PAWLHRPRRG TEGRLETPPE PGPTPGPGVC GPAHWGYALG
110 120 130 140 150
GGGCGPDEYE RRYSGAFPPQ LRAQMRDLAR GMFVFGYDNY MAHAFPQDEL
160 170 180 190 200
NPIYCRGRGP DRGDPSNLNI NDVLGNYSLT LVDALDTLAI MGNSSEFQKA
210 220 230 240 250
VKLVINTVSF DKDSTVQVFE ATIRVLGSLL SAHRIITDSK QPFGDMTIED
260 270 280 290 300
YDNELLYMAH DLAVRLLPAF ENTKTGIPYP RVNLKTGVPP DSNNETCTAG
310 320 330 340 350
AGSLLVEFGI LSRLLGDSTF EWVARRAVKA LWNLRSNDTG LLGNVVNIQT
360 370 380 390 400
GHWVGKQSGL GAGLDSFYEY LLKSYILFGE KEDLEMFNAA YQSIQSYLRR
410 420 430 440 450
GREACNEGEG DPPLYVNVNM FSGQLMNTWI DSLQAFFPGL QVLIGDVEDA
460 470 480 490 500
ICLHAFYYAI WKRYGALPER YNWQLQAPDV LFYPLRPELV ESTYLLYQAT
510 520 530 540 550
KNPFYLHVGM DILQSLEKYT KVKCGYATLH HVIDKSKEDR MESFFLSETC
560 570 580 590 600
KYLYLLFDEE NPVHKSGTRY MFTTEGHIIS VDKRLRELPW KEFFSEDGER
610 620 630 640 650
DQEEKFVHRP KSQELRVINS SSNCNRVPDE RRYSLPLKSI YMRQIDQMVG
LI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SV08 | A0A0N4SV08_MOUSE | alpha-1,2-Mannosidase | Edem1 | 539 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB042828 mRNA Translation: BAB55676.1 AK082920 mRNA Translation: BAC38688.1 BC023237 mRNA Translation: AAH23237.2 |
CCDSi | CCDS20402.1 |
RefSeqi | NP_619618.1, NM_138677.2 |
Genome annotation databases
Ensembli | ENSMUST00000089162; ENSMUSP00000086565; ENSMUSG00000030104 |
GeneIDi | 192193 |
KEGGi | mmu:192193 |
UCSCi | uc009ddq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB042828 mRNA Translation: BAB55676.1 AK082920 mRNA Translation: BAC38688.1 BC023237 mRNA Translation: AAH23237.2 |
CCDSi | CCDS20402.1 |
RefSeqi | NP_619618.1, NM_138677.2 |
3D structure databases
SMRi | Q925U4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228670, 4 interactors |
IntActi | Q925U4, 8 interactors |
STRINGi | 10090.ENSMUSP00000086565 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
PTM databases
GlyGeni | Q925U4, 4 sites |
iPTMneti | Q925U4 |
PhosphoSitePlusi | Q925U4 |
Proteomic databases
EPDi | Q925U4 |
jPOSTi | Q925U4 |
MaxQBi | Q925U4 |
PaxDbi | Q925U4 |
PRIDEi | Q925U4 |
ProteomicsDBi | 277715 |
Protocols and materials databases
Antibodypediai | 25261, 101 antibodies from 27 providers |
DNASUi | 192193 |
Genome annotation databases
Ensembli | ENSMUST00000089162; ENSMUSP00000086565; ENSMUSG00000030104 |
GeneIDi | 192193 |
KEGGi | mmu:192193 |
UCSCi | uc009ddq.1, mouse |
Organism-specific databases
CTDi | 9695 |
MGIi | MGI:2180139, Edem1 |
VEuPathDBi | HostDB:ENSMUSG00000030104 |
Phylogenomic databases
eggNOGi | KOG2429, Eukaryota |
GeneTreei | ENSGT00940000157717 |
HOGENOMi | CLU_003818_5_6_1 |
InParanoidi | Q925U4 |
OMAi | NWTFVKD |
OrthoDBi | 434316at2759 |
PhylomeDBi | Q925U4 |
TreeFami | TF300807 |
Miscellaneous databases
BioGRID-ORCSi | 192193, 0 hits in 64 CRISPR screens |
ChiTaRSi | Edem1, mouse |
PROi | PR:Q925U4 |
RNActi | Q925U4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030104, Expressed in pancreas and 295 other tissues |
ExpressionAtlasi | Q925U4, baseline and differential |
Genevisiblei | Q925U4, MM |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR044674, EDEM1/2/3 IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
PANTHERi | PTHR45679, PTHR45679, 1 hit |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EDEM1_MOUSE | |
Accessioni | Q925U4Primary (citable) accession number: Q925U4 Secondary accession number(s): Q8CFM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families