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UniProtKB - Q925K9 (TSSK6_MOUSE)
Protein
Testis-specific serine/threonine-protein kinase 6
Gene
Tssk6
Organism
Mus musculus (Mouse)
Status
Functioni
Required for sperm production and function. Plays a role in DNA condensation during postmeiotic chromatin remodeling.
1 PublicationCatalytic activityi
Cofactori
Mg2+2 PublicationsNote: Mg2+ and Mn2+ were both present in the kinase buffer but Mg2+ is likely to be the in vivo cofactor.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 41 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 135 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 18 – 26 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein-containing complex binding Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- peptidyl-serine phosphorylation Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- sperm chromatin condensation Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Differentiation, Spermatogenesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 3474 |
Names & Taxonomyi
Protein namesi | Recommended name: Testis-specific serine/threonine-protein kinase 6 (EC:2.7.11.12 Publications)Short name: TSK-6 Short name: TSSK-6 Short name: Testis-specific kinase 6 Alternative name(s): Serine/threonine-protein kinase SSTK Small serine/threonine kinase |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2148775, Tssk6 |
VEuPathDBi | HostDB:ENSMUSG00000047654 |
Pathology & Biotechi
Disruption phenotypei
Mice are sterile due to profound impairment in motility and morphology of spermatozoa.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000227747 | 1 – 273 | Testis-specific serine/threonine-protein kinase 6Add BLAST | 273 |
Post-translational modificationi
Autophosphorylated.By similarity
Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.1 Publication
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q925K9 |
PRIDEi | Q925K9 |
ProteomicsDBi | 300144 |
PTM databases
PhosphoSitePlusi | Q925K9 |
Expressioni
Tissue specificityi
Expressed in the testis, localized to the heads of elongating spermatids.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000047654, Expressed in seminiferous tubule of testis and 74 other tissues |
Genevisiblei | Q925K9, MM |
Interactioni
Subunit structurei
Interacts with HSP90; this interaction stabilizes and activates TSSK6 (PubMed:23599433).
Interacts with the heat shock proteins HSPCB, HSPA8 and HSPA1A. These interactions appear to be required for TSSK6 kinase activity.
Interacts with TSACC; this interaction is direct and recruits TSACC to HSP90, which is essential for kinase activity (By similarity).
By similarity1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000062783 |
Miscellaneous databases
RNActi | Q925K9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 267 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000160464 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | Q925K9 |
OMAi | KEARGYP |
OrthoDBi | 947464at2759 |
PhylomeDBi | Q925K9 |
TreeFami | TF105333 |
Family and domain databases
CDDi | cd14164, STKc_TSSK6-like, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR042710, TSSK6_STKc |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
Q925K9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP
60 70 80 90 100
DFVNKFLPRE LSILRGVRHP HIVHVFEFIE VCNGKLYIVM EAAATDLLQA
110 120 130 140 150
VQRNGRIPGS QARELFSQIA GAVRYLHDHH LVHRDLKCEN VLLSPDERRV
160 170 180 190 200
KITDFGFGRQ AHGYPDLSTT YCGSAAYASP EVLLGIPYDP KKYDVWSLGV
210 220 230 240 250
VLYVMVTGCM PFDDSDIAGL PRRQKRGVLY PDGLELSERC KSLIAELLQF
260 270
SPSARPSAGQ VARNGWLRAG DSG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | L → M in BAC36437 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF329484 mRNA Translation: AAK48828.1 BC049542 mRNA No translation available. AK076653 mRNA Translation: BAC36437.2 |
CCDSi | CCDS22354.1 |
RefSeqi | NP_114393.1, NM_032004.2 |
Genome annotation databases
Ensembli | ENSMUST00000050373; ENSMUSP00000062783; ENSMUSG00000047654 |
GeneIDi | 83984 |
KEGGi | mmu:83984 |
UCSCi | uc009lye.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF329484 mRNA Translation: AAK48828.1 BC049542 mRNA No translation available. AK076653 mRNA Translation: BAC36437.2 |
CCDSi | CCDS22354.1 |
RefSeqi | NP_114393.1, NM_032004.2 |
3D structure databases
SMRi | Q925K9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000062783 |
PTM databases
PhosphoSitePlusi | Q925K9 |
Proteomic databases
PaxDbi | Q925K9 |
PRIDEi | Q925K9 |
ProteomicsDBi | 300144 |
Protocols and materials databases
Antibodypediai | 28486, 170 antibodies from 24 providers |
DNASUi | 83984 |
Genome annotation databases
Ensembli | ENSMUST00000050373; ENSMUSP00000062783; ENSMUSG00000047654 |
GeneIDi | 83984 |
KEGGi | mmu:83984 |
UCSCi | uc009lye.1, mouse |
Organism-specific databases
CTDi | 83983 |
MGIi | MGI:2148775, Tssk6 |
VEuPathDBi | HostDB:ENSMUSG00000047654 |
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000160464 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | Q925K9 |
OMAi | KEARGYP |
OrthoDBi | 947464at2759 |
PhylomeDBi | Q925K9 |
TreeFami | TF105333 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 83984, 0 hits in 64 CRISPR screens |
PROi | PR:Q925K9 |
RNActi | Q925K9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000047654, Expressed in seminiferous tubule of testis and 74 other tissues |
Genevisiblei | Q925K9, MM |
Family and domain databases
CDDi | cd14164, STKc_TSSK6-like, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR042710, TSSK6_STKc |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TSSK6_MOUSE | |
Accessioni | Q925K9Primary (citable) accession number: Q925K9 Secondary accession number(s): Q5RL09, Q8C625 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families