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Protein

Acid-sensing ion channel 2

Gene

Asic2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li+ and K+. Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.1 Publication

Miscellaneous

Regulated by Zn2+. Inhibited by anti-inflammatory drugs like salicylic acid (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Sodium channel
Biological processIon transport, Sodium transport, Transport
LigandSodium

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Protein family/group databases

TCDBi1.A.6.1.8 the epithelial na(+) channel (enac) family

Names & Taxonomyi

Protein namesi
Recommended name:
Acid-sensing ion channel 2
Short name:
ASIC2
Alternative name(s):
Amiloride-sensitive brain sodium channel
Amiloride-sensitive cation channel 1, neuronal
Brain sodium channel 11 Publication
Short name:
BNC1
Short name:
BNaC11 Publication
Gene namesi
Name:Asic2
Synonyms:Accn1, Bnac1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1100867 Asic2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicBy similarityAdd BLAST37
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 427ExtracellularBy similarityAdd BLAST369
Transmembranei428 – 448HelicalSequence analysisAdd BLAST21
Topological domaini449 – 512CytoplasmicBy similarityAdd BLAST64

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display altered rod phototransduction and neurotransmission, associated with increased light-induced retina damages.1 Publication

Chemistry databases

ChEMBLiCHEMBL3232695

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001812911 – 512Acid-sensing ion channel 2Add BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Disulfide bondi92 ↔ 193By similarity
Disulfide bondi171 ↔ 178By similarity
Disulfide bondi289 ↔ 364By similarity
Disulfide bondi307 ↔ 360By similarity
Disulfide bondi311 ↔ 358By similarity
Disulfide bondi320 ↔ 342By similarity
Disulfide bondi322 ↔ 334By similarity
Glycosylationi365N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi392N-linked (GlcNAc...) asparagineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ925H0

PTM databases

PhosphoSitePlusiQ925H0

Expressioni

Tissue specificityi

Expressed by sensory neurons. Expressed by nociceptive sensory neurons, spiral ganglion (SG) neurons and the retina (at protein level). Isoform 1 and isoform 2 are expressed in outer nuclear layer of retina (photoreceptors) and to a lower extent in distal and proximal inner nuclear layer.2 Publications

Developmental stagei

Expression changes dramatically during cochlear development. Expression is detected at E11.5 in otocyst and increases in the SG neurons after E18.5. Also detected in the lumen side of all cells forming the vestibular cavity and at the top of the macula of saccule and utricle. Before birth expressed by epithelial cells facing the endolymphatic space. Post-natally expressed by cells in the apical turn of the cochlea, while expression on the lumen side of the membranous labyrinth decreases. Expression shifts gradually toward the top of supporting cells and the spiral limbus. Also expressed by vestibular ganglion neurons. Restricted to the SG neurons in the mature cochlea (at protein level).1 Publication

Inductioni

Expression in a subset of neurons may be regulated by neurotrophins.1 Publication

Gene expression databases

BgeeiENSMUSG00000020704 Expressed in 123 organ(s), highest expression level in CA1 field of hippocampus
ExpressionAtlasiQ925H0 baseline and differential
GenevisibleiQ925H0 MM

Interactioni

Subunit structurei

Homotrimer or heterotrimer with other ASIC proteins (By similarity). Interacts with PRKCABP and ASIC3 (By similarity). Interacts with STOM; this regulates channel activity (PubMed:15471860, PubMed:22850675). Heterotrimer of Asic1a-Asic2a interacts with the snake venom mambalgin-1, mambalgin-2 and mambalgin-3 (By similarity). Heterotrimer of Asic1a-Asic2b interacts with the snake venom mambalgin-1 and mambalgin-2 (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi197917, 2 interactors
MINTiQ925H0

Structurei

3D structure databases

ProteinModelPortaliQ925H0
SMRiQ925H0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119120
HOGENOMiHOG000247010
HOVERGENiHBG004150
InParanoidiQ925H0
KOiK04828
OMAiHPNTRYE
TreeFamiTF330663

Family and domain databases

InterProiView protein in InterPro
IPR001873 ENaC
IPR004724 ENaC_chordates
IPR020903 ENaC_CS
PANTHERiPTHR11690 PTHR11690, 1 hit
PfamiView protein in Pfam
PF00858 ASC, 1 hit
PRINTSiPR01078 AMINACHANNEL
TIGRFAMsiTIGR00859 ENaC, 1 hit
PROSITEiView protein in PROSITE
PS01206 ASC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q925H0-1) [UniParc]FASTAAdd to basket
Also known as: BNaC1-alpha1 Publication, Asic2a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDLKESPSEG SLQPSSIQIF ANTSTLHGIR HIFVYGPLTI RRVLWAVAFV
60 70 80 90 100
GSLGLLLVES SERVSYYFSY QHVTKVDEVV AQSLVFPAVT LCNLNGFRFS
110 120 130 140 150
RLTTNDLYHA GELLALLDVN LQIPDPHLAD PTVLEALRQK ANFKHYKPKQ
160 170 180 190 200
FSMLEFLHRV GHDLKDMMLY CKFKGQECGH QDFTTVFTKY GKCYMFNSGE
210 220 230 240 250
DGKPLLTTVK GGTGNGLEIM LDIQQDEYLP IWGETEETTF EAGVKVQIHS
260 270 280 290 300
QSEPPFIQEL GFGVAPGFQT FVATQEQRLT YLPPPWGECR SSEMGLDFFP
310 320 330 340 350
VYSITACRID CETRYIVENC NCRMVHMPGD APFCTPEQHK ECAEPALGLL
360 370 380 390 400
AEKDSNYCLC RTPCNLTRYN KELSMVKIPS KTSAKYLEKK FNKSEKYISE
410 420 430 440 450
NILVLDIFFE ALNYETIEQK KAYEVAALLG DIGGQMGLFI GASILTILEL
460 470 480 490 500
FDYIYELIKE KLLDLLGKEE EEGSHDENMS TCDTMPNHSE TISHTVNVPL
510
QTALGTLEEI AC
Length:512
Mass (Da):57,739
Last modified:December 1, 2001 - v1
Checksum:i7D81A77C3B347B04
GO
Isoform 2 (identifier: Q925H0-2) [UniParc]FASTAAdd to basket
Also known as: Mdeg21 Publication, Asic2b

The sequence of this isoform differs from the canonical sequence as follows:
     1-184: Missing.
     185-185: T → MSRSGGARLP...ELCGPHNFSS

Show »
Length:563
Mass (Da):63,204
Checksum:i56F7B4A0A214BA39
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0155921 – 184Missing in isoform 2. 1 PublicationAdd BLAST184
Alternative sequenceiVSP_015593185T → MSRSGGARLPATALSGPGRF RMAREQPAPAAVAAARQPGG DRSGDRELQGPGVARRGRPS LSRTKLHGLRHMCAGRTAAG GSFQRRALWVLAFCTSLGLL LSWSSNRLLYWLSFPSHTRV HREWSRQLPFPAVTVCNNNP LRFPRLSKGDLYYAGHWLGL LLPNRTARPLVSELLRGDEP RRQWFRKLADFRLFLPPRHF EGISAAFMDRLGHQLEDMLL SCKYRGELCGPHNFSS in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14634 mRNA Translation: CAA74978.1
AF348465 mRNA Translation: AAK40101.1
AL645842, AL626807, AL663025 Genomic DNA Translation: CAI24499.1
AL645842, AL645626, AL663025 Genomic DNA Translation: CAI24500.1
AL626807, AL645842, AL663025 Genomic DNA Translation: CAI25306.1
AL645626, AL645842, AL663025 Genomic DNA Translation: CAI25540.1
AL663025, AL626807, AL645842 Genomic DNA Translation: CAI25600.1
AL663025, AL645626, AL645842 Genomic DNA Translation: CAI25602.1
CCDSiCCDS25137.1 [Q925H0-2]
CCDS36243.1 [Q925H0-1]
RefSeqiNP_001029185.1, NM_001034013.2 [Q925H0-1]
NP_031410.1, NM_007384.3 [Q925H0-2]
UniGeneiMm.234998
Mm.259072
Mm.330264
Mm.366419
Mm.392269
Mm.473675

Genome annotation databases

EnsembliENSMUST00000021045; ENSMUSP00000021045; ENSMUSG00000020704 [Q925H0-2]
ENSMUST00000066197; ENSMUSP00000067095; ENSMUSG00000020704 [Q925H0-1]
GeneIDi11418
KEGGimmu:11418
UCSCiuc007kmm.2 mouse [Q925H0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14634 mRNA Translation: CAA74978.1
AF348465 mRNA Translation: AAK40101.1
AL645842, AL626807, AL663025 Genomic DNA Translation: CAI24499.1
AL645842, AL645626, AL663025 Genomic DNA Translation: CAI24500.1
AL626807, AL645842, AL663025 Genomic DNA Translation: CAI25306.1
AL645626, AL645842, AL663025 Genomic DNA Translation: CAI25540.1
AL663025, AL626807, AL645842 Genomic DNA Translation: CAI25600.1
AL663025, AL645626, AL645842 Genomic DNA Translation: CAI25602.1
CCDSiCCDS25137.1 [Q925H0-2]
CCDS36243.1 [Q925H0-1]
RefSeqiNP_001029185.1, NM_001034013.2 [Q925H0-1]
NP_031410.1, NM_007384.3 [Q925H0-2]
UniGeneiMm.234998
Mm.259072
Mm.330264
Mm.366419
Mm.392269
Mm.473675

3D structure databases

ProteinModelPortaliQ925H0
SMRiQ925H0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197917, 2 interactors
MINTiQ925H0

Chemistry databases

ChEMBLiCHEMBL3232695

Protein family/group databases

TCDBi1.A.6.1.8 the epithelial na(+) channel (enac) family

PTM databases

PhosphoSitePlusiQ925H0

Proteomic databases

PRIDEiQ925H0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021045; ENSMUSP00000021045; ENSMUSG00000020704 [Q925H0-2]
ENSMUST00000066197; ENSMUSP00000067095; ENSMUSG00000020704 [Q925H0-1]
GeneIDi11418
KEGGimmu:11418
UCSCiuc007kmm.2 mouse [Q925H0-1]

Organism-specific databases

CTDi40
MGIiMGI:1100867 Asic2

Phylogenomic databases

GeneTreeiENSGT00760000119120
HOGENOMiHOG000247010
HOVERGENiHBG004150
InParanoidiQ925H0
KOiK04828
OMAiHPNTRYE
TreeFamiTF330663

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Miscellaneous databases

PROiPR:Q925H0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020704 Expressed in 123 organ(s), highest expression level in CA1 field of hippocampus
ExpressionAtlasiQ925H0 baseline and differential
GenevisibleiQ925H0 MM

Family and domain databases

InterProiView protein in InterPro
IPR001873 ENaC
IPR004724 ENaC_chordates
IPR020903 ENaC_CS
PANTHERiPTHR11690 PTHR11690, 1 hit
PfamiView protein in Pfam
PF00858 ASC, 1 hit
PRINTSiPR01078 AMINACHANNEL
TIGRFAMsiTIGR00859 ENaC, 1 hit
PROSITEiView protein in PROSITE
PS01206 ASC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASIC2_MOUSE
AccessioniPrimary (citable) accession number: Q925H0
Secondary accession number(s): Q5SUU2, Q61203
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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