UniProtKB - Q925G2 (CYBR1_MOUSE)
Plasma membrane ascorbate-dependent reductase CYBRD1
Cybrd1
Functioni
Plasma membrane reductase that uses cytoplasmic ascorbate as an electron donor to reduce extracellular Fe3+ into Fe2+ (PubMed:17068337).
Probably functions in dietary iron absorption at the brush border of duodenal enterocytes by producing Fe2+, the divalent form of iron that can be transported into enterocytes (PubMed:11230685, PubMed:12547225).
It is also able to reduce extracellular monodehydro-L-ascorbate and may be involved in extracellular ascorbate regeneration by erythrocytes in blood (PubMed:17068337).
May also act as a ferrireductase in airway epithelial cells (By similarity).
May also function as a cupric transmembrane reductase (PubMed:18498772).
By similarity4 PublicationsCatalytic activityi
- EC:7.2.1.3By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- L-ascorbate(in) + monodehydro-L-ascorbate radical(out) = L-ascorbate(out) + monodehydro-L-ascorbate radical(in)By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 50 | Iron 1 (heme b 1 axial ligand); via tele nitrogenBy similarity | 1 | |
Binding sitei | 70 | Heme b 2By similarity | 1 | |
Binding sitei | 79 | AscorbateBy similarity | 1 | |
Binding sitei | 79 | Heme b 2By similarity | 1 | |
Binding sitei | 83 | AscorbateBy similarity | 1 | |
Metal bindingi | 86 | Iron 2 (heme b 2 axial ligand); via tele nitrogenBy similarity | 1 | |
Metal bindingi | 108 | Fe(3+); substrate; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 120 | Iron 1 (heme b 1 axial ligand); via tele nitrogenBy similarity | 1 | |
Binding sitei | 152 | AscorbateBy similarity | 1 | |
Metal bindingi | 159 | Iron 2 (heme b 2 axial ligand); via tele nitrogenBy similarity | 1 | |
Binding sitei | 180 | Heme b 1By similarity | 1 | |
Binding sitei | 225 | Heme b 2By similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity Source: GO_Central
- oxidoreductase activity, acting on metal ions Source: MGI
- transmembrane ascorbate ferrireductase activity Source: UniProtKB
- transmembrane monodehydroascorbate reductase activity Source: UniProtKB
GO - Biological processi
- ascorbate homeostasis Source: UniProtKB
- cellular iron ion homeostasis Source: MGI
- iron ion homeostasis Source: UniProtKB
- response to iron ion Source: MGI
Keywordsi
Molecular function | Oxidoreductase, Translocase |
Biological process | Electron transport, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-917937, Iron uptake and transport |
Names & Taxonomyi
Protein namesi | Recommended name: Plasma membrane ascorbate-dependent reductase CYBRD1By similarity (EC:7.2.1.3By similarity)Alternative name(s): Cytochrome b reductase 1 Duodenal cytochrome b1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2654575, Cybrd1 |
VEuPathDBi | HostDB:ENSMUSG00000027015 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
- Apical cell membrane 2 Publications; Multi-pass membrane protein By similarity
Note: Localized at the brush border of duodenal cells.2 Publications
Lysosome
- lysosomal membrane Source: GO_Central
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- brush border membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicBy similarity | 7 | |
Transmembranei | 8 – 32 | Helical; Name=1By similarityAdd BLAST | 25 | |
Topological domaini | 33 – 47 | ExtracellularBy similarityAdd BLAST | 15 | |
Transmembranei | 48 – 69 | Helical; Name=2By similarityAdd BLAST | 22 | |
Topological domaini | 70 – 78 | CytoplasmicBy similarity | 9 | |
Transmembranei | 79 – 105 | Helical; Name=3By similarityAdd BLAST | 27 | |
Topological domaini | 106 – 118 | ExtracellularBy similarityAdd BLAST | 13 | |
Transmembranei | 119 – 144 | Helical; Name=4By similarityAdd BLAST | 26 | |
Topological domaini | 145 – 151 | CytoplasmicBy similarity | 7 | |
Transmembranei | 152 – 179 | Helical; Name=5By similarityAdd BLAST | 28 | |
Topological domaini | 180 – 197 | ExtracellularBy similarityAdd BLAST | 18 | |
Transmembranei | 198 – 222 | Helical; Name=6By similarityAdd BLAST | 25 | |
Topological domaini | 223 – 290 | CytoplasmicBy similarityAdd BLAST | 68 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000314831 | 1 – 290 | Plasma membrane ascorbate-dependent reductase CYBRD1Add BLAST | 290 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 232 | PhosphoserineBy similarity | 1 | |
Modified residuei | 289 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q925G2 |
PaxDbi | Q925G2 |
PRIDEi | Q925G2 |
ProteomicsDBi | 283995 [Q925G2-1] 283996 [Q925G2-2] |
PTM databases
iPTMneti | Q925G2 |
PhosphoSitePlusi | Q925G2 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000027015, Expressed in epithelium of cochlear duct and 212 other tissues |
Genevisiblei | Q925G2, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000028403 |
Miscellaneous databases
RNActi | Q925G2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 220 | Cytochrome b561PROSITE-ProRule annotationAdd BLAST | 206 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 115 – 118 | Heme b 1 bindingBy similarity | 4 | |
Regioni | 257 – 290 | DisorderedSequence analysisAdd BLAST | 34 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1619, Eukaryota |
GeneTreei | ENSGT00950000183197 |
HOGENOMi | CLU_069712_1_2_1 |
InParanoidi | Q925G2 |
OMAi | TGFIFIQ |
OrthoDBi | 1503869at2759 |
PhylomeDBi | Q925G2 |
TreeFami | TF314222 |
Family and domain databases
InterProi | View protein in InterPro IPR043205, CYB561/CYBRD1-like IPR028838, CYBRD1 IPR006593, Cyt_b561/ferric_Rdtase_TM |
PANTHERi | PTHR10106, PTHR10106, 1 hit PTHR10106:SF12, PTHR10106:SF12, 1 hit |
Pfami | View protein in Pfam PF03188, Cytochrom_B561, 1 hit |
SMARTi | View protein in SMART SM00665, B561, 1 hit |
PROSITEi | View protein in PROSITE PS50939, CYTOCHROME_B561, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAMEGYRGFL GLLVSALLVG FLSVIFVLIW VLHFREGLGW NGSGLEFNWH
60 70 80 90 100
PVLAVTGFVF IQGIAIIVYR LPWTWKCSKL LMKSIHAGLN AVAAILAIIS
110 120 130 140 150
VVAVFEYHNV QKVPHMYSLH SWVGLTALIL YIQQLVVGFF VFLLPWAPPS
160 170 180 190 200
LRAIVMPIHV YSGLLLFGTV IATVLMGVTE KLFFVLKHPS YHSFPPEGVF
210 220 230 240 250
TNTLGLLILV FGALIFWIVT RPQWKRPREP GSVPLQLNGG NAECRMEGAI
260 270 280 290
AISSAHSMDA ADPADAESSS EGAARKRTLG LADSGQRSTM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 188 | H → R in AAK50909 (PubMed:11230685).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030396 | 243 – 244 | EC → D in isoform 2. 1 Publication | 2 | |
Alternative sequenceiVSP_030397 | 261 – 263 | Missing in isoform 2. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF354666 mRNA Translation: AAK50909.1 AK008849 mRNA Translation: BAB25928.1 AK029112 mRNA Translation: BAC26304.1 AL929228 Genomic DNA No translation available. |
CCDSi | CCDS50603.1 [Q925G2-1] |
RefSeqi | NP_082869.2, NM_028593.2 [Q925G2-1] |
Genome annotation databases
Ensembli | ENSMUST00000028403; ENSMUSP00000028403; ENSMUSG00000027015 [Q925G2-1] |
GeneIDi | 73649 |
KEGGi | mmu:73649 |
UCSCi | uc008kaf.2, mouse [Q925G2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF354666 mRNA Translation: AAK50909.1 AK008849 mRNA Translation: BAB25928.1 AK029112 mRNA Translation: BAC26304.1 AL929228 Genomic DNA No translation available. |
CCDSi | CCDS50603.1 [Q925G2-1] |
RefSeqi | NP_082869.2, NM_028593.2 [Q925G2-1] |
3D structure databases
SMRi | Q925G2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000028403 |
PTM databases
iPTMneti | Q925G2 |
PhosphoSitePlusi | Q925G2 |
Proteomic databases
MaxQBi | Q925G2 |
PaxDbi | Q925G2 |
PRIDEi | Q925G2 |
ProteomicsDBi | 283995 [Q925G2-1] 283996 [Q925G2-2] |
Protocols and materials databases
Antibodypediai | 19375, 107 antibodies from 29 providers |
Genome annotation databases
Ensembli | ENSMUST00000028403; ENSMUSP00000028403; ENSMUSG00000027015 [Q925G2-1] |
GeneIDi | 73649 |
KEGGi | mmu:73649 |
UCSCi | uc008kaf.2, mouse [Q925G2-1] |
Organism-specific databases
CTDi | 79901 |
MGIi | MGI:2654575, Cybrd1 |
VEuPathDBi | HostDB:ENSMUSG00000027015 |
Phylogenomic databases
eggNOGi | KOG1619, Eukaryota |
GeneTreei | ENSGT00950000183197 |
HOGENOMi | CLU_069712_1_2_1 |
InParanoidi | Q925G2 |
OMAi | TGFIFIQ |
OrthoDBi | 1503869at2759 |
PhylomeDBi | Q925G2 |
TreeFami | TF314222 |
Enzyme and pathway databases
Reactomei | R-MMU-917937, Iron uptake and transport |
Miscellaneous databases
BioGRID-ORCSi | 73649, 1 hit in 64 CRISPR screens |
ChiTaRSi | Cybrd1, mouse |
PROi | PR:Q925G2 |
RNActi | Q925G2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027015, Expressed in epithelium of cochlear duct and 212 other tissues |
Genevisiblei | Q925G2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR043205, CYB561/CYBRD1-like IPR028838, CYBRD1 IPR006593, Cyt_b561/ferric_Rdtase_TM |
PANTHERi | PTHR10106, PTHR10106, 1 hit PTHR10106:SF12, PTHR10106:SF12, 1 hit |
Pfami | View protein in Pfam PF03188, Cytochrom_B561, 1 hit |
SMARTi | View protein in SMART SM00665, B561, 1 hit |
PROSITEi | View protein in PROSITE PS50939, CYTOCHROME_B561, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CYBR1_MOUSE | |
Accessioni | Q925G2Primary (citable) accession number: Q925G2 Secondary accession number(s): A2AUU8, Q9D7U1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | February 23, 2022 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot