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Protein

Rap guanine nucleotide exchange factor 5

Gene

RAPGEF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.2 Publications

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • Rap guanyl-nucleotide exchange factor activity Source: UniProtKB
  • Ras guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • nervous system development Source: UniProtKB
  • small GTPase mediated signal transduction Source: UniProtKB

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

SIGNORiQ92565

Names & Taxonomyi

Protein namesi
Recommended name:
Rap guanine nucleotide exchange factor 5
Alternative name(s):
Guanine nucleotide exchange factor for Rap1
M-Ras-regulated Rap GEF
Short name:
MR-GEF
Related to Epac
Short name:
Repac
Gene namesi
Name:RAPGEF5
Synonyms:GFR, KIAA0277, MRGEF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000136237.18
HGNCiHGNC:16862 RAPGEF5
MIMi609527 gene
neXtProtiNX_Q92565

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9771
OpenTargetsiENSG00000136237
PharmGKBiPA134902361

Polymorphism and mutation databases

DMDMi32171396

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688731 – 580Rap guanine nucleotide exchange factor 5Add BLAST580

Proteomic databases

MaxQBiQ92565
PaxDbiQ92565
PeptideAtlasiQ92565
PRIDEiQ92565
ProteomicsDBi75322
75323 [Q92565-2]

PTM databases

iPTMnetiQ92565
PhosphoSitePlusiQ92565

Expressioni

Tissue specificityi

Widely expressed with highest levels in brain.

Gene expression databases

BgeeiENSG00000136237 Expressed in 216 organ(s), highest expression level in corpus callosum
CleanExiHS_RAPGEF5
ExpressionAtlasiQ92565 baseline and differential
GenevisibleiQ92565 HS

Organism-specific databases

HPAiHPA057821
HPA076772

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115116, 31 interactors
IntActiQ92565, 3 interactors
STRINGi9606.ENSP00000343656

Structurei

Secondary structure

1580
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92565
SMRiQ92565
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92565

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 201N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST134
Domaini345 – 579Ras-GEFPROSITE-ProRule annotationAdd BLAST235

Phylogenomic databases

eggNOGiKOG2378 Eukaryota
ENOG410XPX9 LUCA
GeneTreeiENSGT00880000137870
HOGENOMiHOG000230545
HOVERGENiHBG056985
InParanoidiQ92565
KOiK08019
PhylomeDBiQ92565

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q92565-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSSRLRVFD PHLERKDSAA ALSDRELPLP TFDVPYFKYI DEEDEDDEWS
60 70 80 90 100
SRSQSSTEDD SVDSLLSDRY VVVSGTPEKI LEHLLNDLHL EEVQDKETET
110 120 130 140 150
LLDDFLLTYT VFMTTDDLCQ ALLRHYSAKK YQGKEENSDV PRRKRKVLHL
160 170 180 190 200
VSQWIALYKD WLPEDEHSKM FLKTIYRNVL DDVYEYPILE KELKEFQKIL
210 220 230 240 250
GMHRRHTVDE YSPQKKNKAL FHQFSLKENW LQHRGTVTET EEIFCHVYIT
260 270 280 290 300
EHSYVSVKAK VSSIAQEILK VVAEKIQYAE EDLALVAITF SGEKHELQPN
310 320 330 340 350
DLVISKSLEA SGRIYVYRKD LADTLNPFAE NEESQQRSMR ILGMNTWDLA
360 370 380 390 400
LELMNFDWSL FNSIHEQELI YFTFSRQGSG EHTANLSLLL QRCNEVQLWV
410 420 430 440 450
ATEILLCSQL GKRVQLVKKF IKIAAHCKAQ RNLNSFFAIV MGLNTASVSR
460 470 480 490 500
LSQTWEKIPG KFKKLFSELE SLTDPSLNHK AYRDAFKKMK PPKIPFMPLL
510 520 530 540 550
LKDVTFIHEG NKTFLDNLVN FEKLHMIADT VRTLRHCRTN QFGDLSPKEH
560 570 580
QELKSYVNHL YVIDSQQALF ELSHRIEPRV
Length:580
Mass (Da):67,733
Last modified:February 1, 1997 - v1
Checksum:i732FB7AA11DFDA1C
GO
Isoform 2 (identifier: Q92565-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-405: Missing.

Note: No experimental confirmation available.
Show »
Length:444
Mass (Da):52,083
Checksum:iF615DF931DCEF02B
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A8MQ07A8MQ07_HUMAN
Rap guanine nucleotide exchange fac...
RAPGEF5
730Annotation score:
C9JBS6C9JBS6_HUMAN
Rap guanine nucleotide exchange fac...
RAPGEF5
142Annotation score:
E9PGY3E9PGY3_HUMAN
Rap guanine nucleotide exchange fac...
RAPGEF5
400Annotation score:
C9JHM4C9JHM4_HUMAN
Rap guanine nucleotide exchange fac...
RAPGEF5
155Annotation score:
F8WD32F8WD32_HUMAN
Rap guanine nucleotide exchange fac...
RAPGEF5
124Annotation score:

Sequence cautioni

The sequence BAA13406 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti497M → V in AAH39203 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007616270 – 405Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87467 mRNA Translation: BAA13406.2 Different initiation.
CH236948 Genomic DNA Translation: EAL24270.1
BC039203 mRNA Translation: AAH39203.1
RefSeqiNP_036426.3, NM_012294.3
UniGeneiHs.174768
Hs.713217

Genome annotation databases

EnsembliENST00000401957; ENSP00000384044; ENSG00000136237 [Q92565-1]
ENST00000620335; ENSP00000479340; ENSG00000136237 [Q92565-2]
GeneIDi9771
KEGGihsa:9771
UCSCiuc011jym.2 human [Q92565-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87467 mRNA Translation: BAA13406.2 Different initiation.
CH236948 Genomic DNA Translation: EAL24270.1
BC039203 mRNA Translation: AAH39203.1
RefSeqiNP_036426.3, NM_012294.3
UniGeneiHs.174768
Hs.713217

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGYNMR-A241-331[»]
ProteinModelPortaliQ92565
SMRiQ92565
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115116, 31 interactors
IntActiQ92565, 3 interactors
STRINGi9606.ENSP00000343656

PTM databases

iPTMnetiQ92565
PhosphoSitePlusiQ92565

Polymorphism and mutation databases

DMDMi32171396

Proteomic databases

MaxQBiQ92565
PaxDbiQ92565
PeptideAtlasiQ92565
PRIDEiQ92565
ProteomicsDBi75322
75323 [Q92565-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000401957; ENSP00000384044; ENSG00000136237 [Q92565-1]
ENST00000620335; ENSP00000479340; ENSG00000136237 [Q92565-2]
GeneIDi9771
KEGGihsa:9771
UCSCiuc011jym.2 human [Q92565-1]

Organism-specific databases

CTDi9771
DisGeNETi9771
EuPathDBiHostDB:ENSG00000136237.18
GeneCardsiRAPGEF5
H-InvDBiHIX0006511
HGNCiHGNC:16862 RAPGEF5
HPAiHPA057821
HPA076772
MIMi609527 gene
neXtProtiNX_Q92565
OpenTargetsiENSG00000136237
PharmGKBiPA134902361
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2378 Eukaryota
ENOG410XPX9 LUCA
GeneTreeiENSGT00880000137870
HOGENOMiHOG000230545
HOVERGENiHBG056985
InParanoidiQ92565
KOiK08019
PhylomeDBiQ92565

Enzyme and pathway databases

SIGNORiQ92565

Miscellaneous databases

ChiTaRSiRAPGEF5 human
EvolutionaryTraceiQ92565
GeneWikiiRAPGEF5
GenomeRNAii9771
PROiPR:Q92565
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136237 Expressed in 216 organ(s), highest expression level in corpus callosum
CleanExiHS_RAPGEF5
ExpressionAtlasiQ92565 baseline and differential
GenevisibleiQ92565 HS

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPGF5_HUMAN
AccessioniPrimary (citable) accession number: Q92565
Secondary accession number(s): A4D140, Q8IXU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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