UniProtKB - Q92564 (DCNL4_HUMAN)
DCN1-like protein 4
DCUN1D4
Functioni
Contributes to the neddylation of all cullins by transferring NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which are necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs).
2 PublicationsGO - Molecular functioni
- cullin family protein binding Source: UniProtKB
- ubiquitin conjugating enzyme binding Source: GO_Central
- ubiquitin-like protein binding Source: GO_Central
GO - Biological processi
- positive regulation of protein neddylation Source: UniProtKB
- positive regulation of ubiquitin-protein transferase activity Source: GO_Central
- protein neddylation Source: GO_Central
Enzyme and pathway databases
PathwayCommonsi | Q92564 |
Reactomei | R-HSA-8951664, Neddylation |
SignaLinki | Q92564 |
Names & Taxonomyi
Protein namesi | Recommended name: DCN1-like protein 4CuratedShort name: DCNL41 Publication Alternative name(s): DCUN1 domain-containing protein 4 Defective in cullin neddylation protein 1-like protein 4 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28998, DCUN1D4 |
MIMi | 612977, gene |
neXtProti | NX_Q92564 |
VEuPathDBi | HostDB:ENSG00000109184 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: LIFEdb
Other locations
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 250 | D → A: Does not affect localization at nucleus; when associated with R-274 and A-280. 1 Publication | 1 | |
Mutagenesisi | 274 | A → R: Does not affect localization at nucleus; when associated with A-250 and A-280. 1 Publication | 1 | |
Mutagenesisi | 280 | D → A: Does not affect localization at nucleus; when associated with A-250 and R-274. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23142 |
OpenTargetsi | ENSG00000109184 |
PharmGKBi | PA142672010 |
Miscellaneous databases
Pharosi | Q92564, Tdark |
Chemistry databases
ChEMBLi | CHEMBL4295914 |
Genetic variation databases
BioMutai | DCUN1D4 |
DMDMi | 78099237 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000129503 | 1 – 292 | DCN1-like protein 4Add BLAST | 292 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 95 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q92564 |
jPOSTi | Q92564 |
MassIVEi | Q92564 |
MaxQBi | Q92564 |
PaxDbi | Q92564 |
PeptideAtlasi | Q92564 |
PRIDEi | Q92564 |
ProteomicsDBi | 4184 75320 [Q92564-1] 75321 [Q92564-2] |
PTM databases
iPTMneti | Q92564 |
PhosphoSitePlusi | Q92564 |
Expressioni
Gene expression databases
Bgeei | ENSG00000109184, Expressed in calcaneal tendon and 237 other tissues |
ExpressionAtlasi | Q92564, baseline and differential |
Genevisiblei | Q92564, HS |
Organism-specific databases
HPAi | ENSG00000109184, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (via the DCUN1 domain) with the unneddylated cullins: interacts with CUL1, CUL2, CUL3, CUL4A, CUL4B and CUL5; these interactions promote the cullin neddylation and the identity of the cullin dictates the affinity of the interaction (PubMed:26906416, PubMed:23201271).
Interacts with RBX1 and RNF7 (PubMed:26906416).
Interacts with CAND1; this interaction is bridged by cullins such as CUL3 and strongly inhibits the neddylation of CUL3. These CAND-cullin-DCNL complexes can only be neddylated in the presence of a substrate adapter (PubMed:26906416).
Interacts (via DCUN1 domain) with UBE2M (N-terminally acetylated form) and probably with UBE2F (N-terminally acetylated form) (PubMed:23201271).
2 PublicationsBinary interactionsi
Q92564
With | #Exp. | IntAct |
---|---|---|
RNA-directed RNA polymerase NS5 (PRO_0000037946) from Dengue virus type 2 (strain 16681-PDK53). | 3 | EBI-2654610,EBI-8826488 |
GO - Molecular functioni
- cullin family protein binding Source: UniProtKB
- ubiquitin conjugating enzyme binding Source: GO_Central
- ubiquitin-like protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 116759, 22 interactors |
IntActi | Q92564, 4 interactors |
STRINGi | 9606.ENSP00000389900 |
Chemistry databases
BindingDBi | Q92564 |
Miscellaneous databases
RNActi | Q92564, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q92564 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 101 – 287 | DCUN1PROSITE-ProRule annotationAdd BLAST | 187 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 43 – 83 | DisorderedSequence analysisAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 43 – 57 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 60 – 83 | Basic and acidic residuesSequence analysisAdd BLAST | 24 |
Domaini
Phylogenomic databases
eggNOGi | KOG3077, Eukaryota |
GeneTreei | ENSGT00940000156935 |
HOGENOMi | CLU_047042_3_1_1 |
InParanoidi | Q92564 |
OMAi | VEWYKEK |
OrthoDBi | 1418097at2759 |
PhylomeDBi | Q92564 |
TreeFami | TF354270 |
Family and domain databases
Gene3Di | 1.10.238.200, 1 hit |
InterProi | View protein in InterPro IPR014764, DCN-prot IPR042460, DCN1-like_PONY IPR005176, PONY_dom |
PANTHERi | PTHR12281, PTHR12281, 1 hit |
Pfami | View protein in Pfam PF03556, Cullin_binding, 1 hit |
PROSITEi | View protein in PROSITE PS51229, DCUN1, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHSDAAAVNF QLNSHLSTLA NIHKIYHTLN KLNLTEDIGQ DDHQTGSLRS
60 70 80 90 100
CSSSDCFNKV MPPRKKRRPA SGDDLSAKKS RHDSMYRKYD STRIKTEEEA
110 120 130 140 150
FSSKRCLEWF YEYAGTDDVV GPEGMEKFCE DIGVEPENVV MLVLAWKLDA
160 170 180 190 200
QNMGYFTLQE WLKGMTSLQC DTTEKLRNTL DYLRSFLNDS TNFKLIYRYA
210 220 230 240 250
FDFAREKDQR SLDINTAKCM LGLLLGKIWP LFPVFHQFLE QSKYKVINKD
260 270 280 290
QWCNVLEFSR TINLDLSNYD EDGAWPVLLD EFVEWYKDKQ MS
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RDV8 | D6RDV8_HUMAN | DCN1-like protein | DCUN1D4 | 212 | Annotation score: | ||
D6RB33 | D6RB33_HUMAN | DCN1-like protein | DCUN1D4 hCG_21420 | 143 | Annotation score: | ||
D6RAL8 | D6RAL8_HUMAN | DCN1-like protein | DCUN1D4 | 207 | Annotation score: | ||
H0YA35 | H0YA35_HUMAN | DCN1-like protein | DCUN1D4 | 102 | Annotation score: | ||
H0Y907 | H0Y907_HUMAN | DCN1-like protein | DCUN1D4 | 116 | Annotation score: | ||
D6RGX7 | D6RGX7_HUMAN | DCN1-like protein 4 | DCUN1D4 | 63 | Annotation score: | ||
H0Y9C5 | H0Y9C5_HUMAN | DCN1-like protein 4 | DCUN1D4 | 21 | Annotation score: | ||
D6RCB7 | D6RCB7_HUMAN | DCN1-like protein 4 | DCUN1D4 | 24 | Annotation score: | ||
D6RIY3 | D6RIY3_HUMAN | DCN1-like protein 4 | DCUN1D4 | 46 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054381 | 1 – 9 | MHSDAAAVN → MEVEAALGCSGQGRGCGGVA PAGRGRERASERGTRVRISK GLSGAGGSVRKAD in isoform 3. 1 Publication | 9 | |
Alternative sequenceiVSP_016016 | 206 – 240 | Missing in isoform 2. 1 PublicationAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87466 mRNA Translation: BAA13405.1 Different initiation. AK294894 mRNA Translation: BAG57986.1 BX537944 mRNA Translation: CAD97912.1 Different initiation. AC027271 Genomic DNA No translation available. BC053897 mRNA Translation: AAH53897.2 |
CCDSi | CCDS33982.1 [Q92564-1] CCDS3487.2 [Q92564-2] CCDS75123.1 [Q92564-3] |
RefSeqi | NP_001035492.1, NM_001040402.2 [Q92564-1] NP_001274684.1, NM_001287755.1 [Q92564-3] NP_001274686.1, NM_001287757.1 NP_055930.2, NM_015115.3 [Q92564-2] |
Genome annotation databases
Ensembli | ENST00000334635; ENSP00000334625; ENSG00000109184 ENST00000381441; ENSP00000370850; ENSG00000109184 [Q92564-2] ENST00000451288; ENSP00000389900; ENSG00000109184 [Q92564-3] |
GeneIDi | 23142 |
KEGGi | hsa:23142 |
MANE-Selecti | ENST00000334635.10; ENSP00000334625.5; NM_001040402.3; NP_001035492.1 |
UCSCi | uc003gze.5, human [Q92564-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87466 mRNA Translation: BAA13405.1 Different initiation. AK294894 mRNA Translation: BAG57986.1 BX537944 mRNA Translation: CAD97912.1 Different initiation. AC027271 Genomic DNA No translation available. BC053897 mRNA Translation: AAH53897.2 |
CCDSi | CCDS33982.1 [Q92564-1] CCDS3487.2 [Q92564-2] CCDS75123.1 [Q92564-3] |
RefSeqi | NP_001035492.1, NM_001040402.2 [Q92564-1] NP_001274684.1, NM_001287755.1 [Q92564-3] NP_001274686.1, NM_001287757.1 NP_055930.2, NM_015115.3 [Q92564-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5V89 | X-ray | 1.55 | A | 102-292 | [»] | |
SMRi | Q92564 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116759, 22 interactors |
IntActi | Q92564, 4 interactors |
STRINGi | 9606.ENSP00000389900 |
Chemistry databases
BindingDBi | Q92564 |
ChEMBLi | CHEMBL4295914 |
PTM databases
iPTMneti | Q92564 |
PhosphoSitePlusi | Q92564 |
Genetic variation databases
BioMutai | DCUN1D4 |
DMDMi | 78099237 |
Proteomic databases
EPDi | Q92564 |
jPOSTi | Q92564 |
MassIVEi | Q92564 |
MaxQBi | Q92564 |
PaxDbi | Q92564 |
PeptideAtlasi | Q92564 |
PRIDEi | Q92564 |
ProteomicsDBi | 4184 75320 [Q92564-1] 75321 [Q92564-2] |
Protocols and materials databases
Antibodypediai | 23882, 109 antibodies from 17 providers |
DNASUi | 23142 |
Genome annotation databases
Ensembli | ENST00000334635; ENSP00000334625; ENSG00000109184 ENST00000381441; ENSP00000370850; ENSG00000109184 [Q92564-2] ENST00000451288; ENSP00000389900; ENSG00000109184 [Q92564-3] |
GeneIDi | 23142 |
KEGGi | hsa:23142 |
MANE-Selecti | ENST00000334635.10; ENSP00000334625.5; NM_001040402.3; NP_001035492.1 |
UCSCi | uc003gze.5, human [Q92564-1] |
Organism-specific databases
CTDi | 23142 |
DisGeNETi | 23142 |
GeneCardsi | DCUN1D4 |
HGNCi | HGNC:28998, DCUN1D4 |
HPAi | ENSG00000109184, Low tissue specificity |
MIMi | 612977, gene |
neXtProti | NX_Q92564 |
OpenTargetsi | ENSG00000109184 |
PharmGKBi | PA142672010 |
VEuPathDBi | HostDB:ENSG00000109184 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3077, Eukaryota |
GeneTreei | ENSGT00940000156935 |
HOGENOMi | CLU_047042_3_1_1 |
InParanoidi | Q92564 |
OMAi | VEWYKEK |
OrthoDBi | 1418097at2759 |
PhylomeDBi | Q92564 |
TreeFami | TF354270 |
Enzyme and pathway databases
PathwayCommonsi | Q92564 |
Reactomei | R-HSA-8951664, Neddylation |
SignaLinki | Q92564 |
Miscellaneous databases
BioGRID-ORCSi | 23142, 10 hits in 1047 CRISPR screens |
ChiTaRSi | DCUN1D4, human |
GeneWikii | DCUN1D4 |
GenomeRNAii | 23142 |
Pharosi | Q92564, Tdark |
PROi | PR:Q92564 |
RNActi | Q92564, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000109184, Expressed in calcaneal tendon and 237 other tissues |
ExpressionAtlasi | Q92564, baseline and differential |
Genevisiblei | Q92564, HS |
Family and domain databases
Gene3Di | 1.10.238.200, 1 hit |
InterProi | View protein in InterPro IPR014764, DCN-prot IPR042460, DCN1-like_PONY IPR005176, PONY_dom |
PANTHERi | PTHR12281, PTHR12281, 1 hit |
Pfami | View protein in Pfam PF03556, Cullin_binding, 1 hit |
PROSITEi | View protein in PROSITE PS51229, DCUN1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCNL4_HUMAN | |
Accessioni | Q92564Primary (citable) accession number: Q92564 Secondary accession number(s): B4DH25, Q7Z3F3, Q7Z6B8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references