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Protein

Wiskott-Aldrich syndrome protein family member 1

Gene

WASF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).By similarity1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • protein-containing complex binding Source: UniProtKB
  • protein kinase A binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

ReactomeiR-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-4420097 VEGFA-VEGFR2 Pathway
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
SIGNORiQ92558

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 1
Short name:
WASP family protein member 1
Alternative name(s):
Protein WAVE-1
Verprolin homology domain-containing protein 1
Gene namesi
Name:WASF1
Synonyms:KIAA0269, SCAR1, WAVE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000112290.12
HGNCiHGNC:12732 WASF1
MIMi605035 gene
neXtProtiNX_Q92558

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi8936
OpenTargetsiENSG00000112290
PharmGKBiPA37343

Polymorphism and mutation databases

BioMutaiWASF1
DMDMi2495730

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889921 – 559Wiskott-Aldrich syndrome protein family member 1Add BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei341Omega-N-methylarginine; alternateBy similarity1
Modified residuei489PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ92558
MaxQBiQ92558
PaxDbiQ92558
PeptideAtlasiQ92558
PRIDEiQ92558
ProteomicsDBi75315

PTM databases

iPTMnetiQ92558
PhosphoSitePlusiQ92558

Miscellaneous databases

PMAP-CutDBiQ92558

Expressioni

Tissue specificityi

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Gene expression databases

BgeeiENSG00000112290 Expressed in 209 organ(s), highest expression level in cerebral cortex
CleanExiHS_WASF1
ExpressionAtlasiQ92558 baseline and differential
GenevisibleiQ92558 HS

Organism-specific databases

HPAiCAB022161
HPA004105

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114449, 47 interactors
DIPiDIP-39391N
ELMiQ92558
IntActiQ92558, 39 interactors
MINTiQ92558
STRINGi9606.ENSP00000352425

Structurei

Secondary structure

1559
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92558
SMRiQ92558
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92558

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini497 – 514WH2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 283Poly-Pro6
Compositional biasi322 – 332Poly-ProAdd BLAST11
Compositional biasi348 – 359Poly-ProAdd BLAST12
Compositional biasi369 – 374Poly-Pro6
Compositional biasi424 – 435Poly-ProAdd BLAST12

Domaini

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.1 Publication

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated

Phylogenomic databases

eggNOGiKOG1830 Eukaryota
ENOG410XRBQ LUCA
GeneTreeiENSGT00730000110593
HOGENOMiHOG000021456
HOVERGENiHBG058482
InParanoidiQ92558
KOiK05753
OMAiDEGDWME
OrthoDBiEOG091G08O1
PhylomeDBiQ92558
TreeFamiTF315031

Family and domain databases

InterProiView protein in InterPro
IPR028288 SCAR/WAVE_fam
IPR003124 WH2_dom
PANTHERiPTHR12902 PTHR12902, 1 hit
PfamiView protein in Pfam
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM00246 WH2, 1 hit
PROSITEiView protein in PROSITE
PS51082 WH2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q92558-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED
60 70 80 90 100
IFGELFNEAH SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA
110 120 130 140 150
FRSSTIQDQQ LFDRKTLPIP LQETYDVCEQ PPPLNILTPY RDDGKEGLKF
160 170 180 190 200
YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK QKNLDRPHEP EKVPRAPHDR
210 220 230 240 250
RREWQKLAQG PELAEDDANL LHKHIEVANG PASHFETRPQ TYVDHMDGSY
260 270 280 290 300
SLSALPFSQM SELLTRAEER VLVRPHEPPP PPPMHGAGDA KPIPTCISSA
310 320 330 340 350
TGLIENRPQS PATGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP
360 370 380 390 400
PVPPPPPPPA TALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV
410 420 430 440 450
ARAAPVCETV PVHPLPQGEV QGLPPPPPPP PLPPPGIRPS SPVTVTALAH
460 470 480 490 500
PPSGLHPTPS TAPGPHVPLM PPSPPSQVIP ASEPKRHPST LPVISDARSV
510 520 530 540 550
LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA VEYSDSEDDS

EFDEVDWLE
Length:559
Mass (Da):61,652
Last modified:February 1, 1997 - v1
Checksum:i44B4527BDB77BC6E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5SZK5Q5SZK5_HUMAN
Wiskott-Aldrich syndrome protein fa...
WASF1
180Annotation score:
Q5SZK4Q5SZK4_HUMAN
Wiskott-Aldrich syndrome protein fa...
WASF1
140Annotation score:
Q5SZK3Q5SZK3_HUMAN
Wiskott-Aldrich syndrome protein fa...
WASF1
89Annotation score:

Sequence cautioni

The sequence BAA13399 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134303 mRNA Translation: AAD33052.1
D87459 mRNA Translation: BAA13399.2 Different initiation.
AL590009 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48325.1
CH471051 Genomic DNA Translation: EAW48326.1
CH471051 Genomic DNA Translation: EAW48327.1
CH471051 Genomic DNA Translation: EAW48328.1
CH471051 Genomic DNA Translation: EAW48329.1
BC044591 mRNA Translation: AAH44591.1
CCDSiCCDS5080.1
RefSeqiNP_001020105.1, NM_001024934.1
NP_001020106.1, NM_001024935.1
NP_001020107.1, NM_001024936.1
NP_003922.1, NM_003931.2
XP_005267260.1, XM_005267203.3
XP_005267262.1, XM_005267205.2
XP_005267263.1, XM_005267206.2
XP_005267264.1, XM_005267207.2
XP_011534535.1, XM_011536233.1
XP_011534536.1, XM_011536234.1
XP_016866926.1, XM_017011437.1
XP_016866927.1, XM_017011438.1
UniGeneiHs.75850

Genome annotation databases

EnsembliENST00000359451; ENSP00000352425; ENSG00000112290
ENST00000392586; ENSP00000376365; ENSG00000112290
ENST00000392587; ENSP00000376366; ENSG00000112290
ENST00000392588; ENSP00000376367; ENSG00000112290
ENST00000392589; ENSP00000376368; ENSG00000112290
GeneIDi8936
KEGGihsa:8936
UCSCiuc003ptv.1 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134303 mRNA Translation: AAD33052.1
D87459 mRNA Translation: BAA13399.2 Different initiation.
AL590009 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48325.1
CH471051 Genomic DNA Translation: EAW48326.1
CH471051 Genomic DNA Translation: EAW48327.1
CH471051 Genomic DNA Translation: EAW48328.1
CH471051 Genomic DNA Translation: EAW48329.1
BC044591 mRNA Translation: AAH44591.1
CCDSiCCDS5080.1
RefSeqiNP_001020105.1, NM_001024934.1
NP_001020106.1, NM_001024935.1
NP_001020107.1, NM_001024936.1
NP_003922.1, NM_003931.2
XP_005267260.1, XM_005267203.3
XP_005267262.1, XM_005267205.2
XP_005267263.1, XM_005267206.2
XP_005267264.1, XM_005267207.2
XP_011534535.1, XM_011536233.1
XP_011534536.1, XM_011536234.1
XP_016866926.1, XM_017011437.1
XP_016866927.1, XM_017011438.1
UniGeneiHs.75850

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P8CX-ray2.29D1-186[»]
D485-559[»]
4N78X-ray2.43D1-559[»]
ProteinModelPortaliQ92558
SMRiQ92558
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114449, 47 interactors
DIPiDIP-39391N
ELMiQ92558
IntActiQ92558, 39 interactors
MINTiQ92558
STRINGi9606.ENSP00000352425

PTM databases

iPTMnetiQ92558
PhosphoSitePlusiQ92558

Polymorphism and mutation databases

BioMutaiWASF1
DMDMi2495730

Proteomic databases

EPDiQ92558
MaxQBiQ92558
PaxDbiQ92558
PeptideAtlasiQ92558
PRIDEiQ92558
ProteomicsDBi75315

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359451; ENSP00000352425; ENSG00000112290
ENST00000392586; ENSP00000376365; ENSG00000112290
ENST00000392587; ENSP00000376366; ENSG00000112290
ENST00000392588; ENSP00000376367; ENSG00000112290
ENST00000392589; ENSP00000376368; ENSG00000112290
GeneIDi8936
KEGGihsa:8936
UCSCiuc003ptv.1 human

Organism-specific databases

CTDi8936
DisGeNETi8936
EuPathDBiHostDB:ENSG00000112290.12
GeneCardsiWASF1
HGNCiHGNC:12732 WASF1
HPAiCAB022161
HPA004105
MIMi605035 gene
neXtProtiNX_Q92558
OpenTargetsiENSG00000112290
PharmGKBiPA37343
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1830 Eukaryota
ENOG410XRBQ LUCA
GeneTreeiENSGT00730000110593
HOGENOMiHOG000021456
HOVERGENiHBG058482
InParanoidiQ92558
KOiK05753
OMAiDEGDWME
OrthoDBiEOG091G08O1
PhylomeDBiQ92558
TreeFamiTF315031

Enzyme and pathway databases

ReactomeiR-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-4420097 VEGFA-VEGFR2 Pathway
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
SIGNORiQ92558

Miscellaneous databases

ChiTaRSiWASF1 human
EvolutionaryTraceiQ92558
GeneWikiiWASF1
GenomeRNAii8936
PMAP-CutDBiQ92558
PROiPR:Q92558
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112290 Expressed in 209 organ(s), highest expression level in cerebral cortex
CleanExiHS_WASF1
ExpressionAtlasiQ92558 baseline and differential
GenevisibleiQ92558 HS

Family and domain databases

InterProiView protein in InterPro
IPR028288 SCAR/WAVE_fam
IPR003124 WH2_dom
PANTHERiPTHR12902 PTHR12902, 1 hit
PfamiView protein in Pfam
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM00246 WH2, 1 hit
PROSITEiView protein in PROSITE
PS51082 WH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWASF1_HUMAN
AccessioniPrimary (citable) accession number: Q92558
Secondary accession number(s): E1P5F2, Q5SZK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 10, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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