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Protein

Proteasome inhibitor PI31 subunit

Gene

PSMF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.1 Publication

GO - Molecular functioni

  • endopeptidase inhibitor activity Source: UniProtKB
  • proteasome binding Source: UniProtKB
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • protein homodimerization activity Source: ParkinsonsUK-UCL

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-9604323 Negative regulation of NOTCH4 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome inhibitor PI31 subunit
Short name:
hPI31
Gene namesi
Name:PSMF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000125818.17
HGNCiHGNC:9571 PSMF1
MIMi617858 gene
neXtProtiNX_Q92530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6V → R: Abolishes homodimerization. 1 Publication1
Mutagenesisi83I → E: Abolishes interaction with FBXO7, but has no effect on homodimerization; when associated with E-90. 1 Publication1
Mutagenesisi90I → E: Abolishes interaction with FBXO7, but has no effect on homodimerization; when associated with E-83. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000125818
PharmGKBiPA33917

Polymorphism and mutation databases

BioMutaiPSMF1
DMDMi134047876

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002209202 – 271Proteasome inhibitor PI31 subunitAdd BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei153PhosphoserineCombined sources1
Modified residuei205Omega-N-methylarginineBy similarity1
Modified residuei219Asymmetric dimethylarginineBy similarity1
Modified residuei231Omega-N-methylarginineCombined sources1
Modified residuei252PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ92530
MaxQBiQ92530
PaxDbiQ92530
PeptideAtlasiQ92530
PRIDEiQ92530
ProteomicsDBi75291

PTM databases

iPTMnetiQ92530
PhosphoSitePlusiQ92530
SwissPalmiQ92530

Expressioni

Gene expression databases

BgeeiENSG00000125818 Expressed in 235 organ(s), highest expression level in testis
CleanExiHS_PSMF1
ExpressionAtlasiQ92530 baseline and differential
GenevisibleiQ92530 HS

Organism-specific databases

HPAiHPA041122
HPA041300

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with FBXO7. Interacts with the 20S proteasome.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114872, 56 interactors
IntActiQ92530, 31 interactors
MINTiQ92530
STRINGi9606.ENSP00000327704

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92530
SMRiQ92530
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92530

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 150Important for homodimerization and interaction with FBXO71 PublicationAdd BLAST149

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi154 – 271Pro-richAdd BLAST118

Sequence similaritiesi

Belongs to the proteasome inhibitor PI31 family.Curated

Phylogenomic databases

eggNOGiKOG4761 Eukaryota
ENOG4111GK4 LUCA
GeneTreeiENSGT00390000012257
HOGENOMiHOG000231957
HOVERGENiHBG053746
InParanoidiQ92530
KOiK06700
OMAiRPFGFPD
OrthoDBiEOG091G0JIA
PhylomeDBiQ92530
TreeFamiTF106238

Family and domain databases

InterProiView protein in InterPro
IPR013886 PI31_Prot_C
IPR021625 PI31_Prot_N
PfamiView protein in Pfam
PF08577 PI31_Prot_C, 1 hit
PF11566 PI31_Prot_N, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.iShow all

Q92530-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYFGLGV GDQPGPNDKK
60 70 80 90 100
SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ
110 120 130 140 150
VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN
160 170 180 190 200
VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP
210 220 230 240 250
FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG
260 270
TSPPGPNPDH LPPPGYDDMY L
Length:271
Mass (Da):29,817
Last modified:March 20, 2007 - v2
Checksum:iC669F8210AFAD727
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5QPM7Q5QPM7_HUMAN
Proteasome inhibitor PI31 subunit
PSMF1
263Annotation score:
Q5QPM9Q5QPM9_HUMAN
Proteasome inhibitor PI31 subunit
PSMF1
183Annotation score:
H0Y555H0Y555_HUMAN
Proteasome inhibitor PI31 subunit
PSMF1
107Annotation score:
F5H4Z3F5H4Z3_HUMAN
Proteasome inhibitor PI31 subunit
PSMF1
127Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02456436F → C3 PublicationsCorresponds to variant dbSNP:rs1803415Ensembl.1
Natural variantiVAR_022153174H → R. Corresponds to variant dbSNP:rs2235587Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88378 mRNA Translation: BAA13603.1
AL031665 Genomic DNA No translation available.
CH471133 Genomic DNA Translation: EAX10650.1
CH471133 Genomic DNA Translation: EAX10651.1
BC126462 mRNA Translation: AAI26463.1
CCDSiCCDS13010.1
RefSeqiNP_001310336.1, NM_001323407.1
NP_006805.2, NM_006814.4
NP_848693.2, NM_178578.3
UniGeneiHs.471917
Hs.656042

Genome annotation databases

EnsembliENST00000333082; ENSP00000327704; ENSG00000125818
ENST00000335877; ENSP00000338039; ENSG00000125818
GeneIDi9491
KEGGihsa:9491
UCSCiuc002wel.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPSMF1_HUMAN
AccessioniPrimary (citable) accession number: Q92530
Secondary accession number(s): A0AVQ9, D3DVW3, Q9H4I1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 20, 2007
Last modified: September 12, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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