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UniProtKB - Q92530 (PSMF1_HUMAN)
Protein
Proteasome inhibitor PI31 subunit
Gene
PSMF1
Organism
Homo sapiens (Human)
Status
Functioni
Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
1 PublicationGO - Molecular functioni
- endopeptidase inhibitor activity Source: UniProtKB
- proteasome binding Source: UniProtKB
- protein heterodimerization activity Source: ParkinsonsUK-UCL
- protein homodimerization activity Source: ParkinsonsUK-UCL
GO - Biological processi
- negative regulation of proteasomal protein catabolic process Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: InterPro
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q92530 |
Reactomei | R-HSA-1169091, Activation of NF-kappaB in B cells R-HSA-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-HSA-1236974, ER-Phagosome pathway R-HSA-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-HSA-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174113, SCF-beta-TrCP mediated degradation of Emi1 R-HSA-174154, APC/C:Cdc20 mediated degradation of Securin R-HSA-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-180534, Vpu mediated degradation of CD4 R-HSA-180585, Vif-mediated degradation of APOBEC3G R-HSA-187577, SCF(Skp2)-mediated degradation of p27/p21 R-HSA-195253, Degradation of beta-catenin by the destruction complex R-HSA-202424, Downstream TCR signaling R-HSA-211733, Regulation of activated PAK-2p34 by proteasome mediated degradation R-HSA-2467813, Separation of Sister Chromatids R-HSA-2871837, FCERI mediated NF-kB activation R-HSA-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-HSA-350562, Regulation of ornithine decarboxylase (ODC) R-HSA-382556, ABC-family proteins mediated transport R-HSA-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-HSA-4608870, Asymmetric localization of PCP proteins R-HSA-4641257, Degradation of AXIN R-HSA-4641258, Degradation of DVL R-HSA-5358346, Hedgehog ligand biogenesis R-HSA-5362768, Hh mutants are degraded by ERAD R-HSA-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5607764, CLEC7A (Dectin-1) signaling R-HSA-5610780, Degradation of GLI1 by the proteasome R-HSA-5610783, Degradation of GLI2 by the proteasome R-HSA-5610785, GLI3 is processed to GLI3R by the proteasome R-HSA-5632684, Hedgehog 'on' state R-HSA-5658442, Regulation of RAS by GAPs R-HSA-5668541, TNFR2 non-canonical NF-kB pathway R-HSA-5676590, NIK-->noncanonical NF-kB signaling R-HSA-5678895, Defective CFTR causes cystic fibrosis R-HSA-5687128, MAPK6/MAPK4 signaling R-HSA-5689603, UCH proteinases R-HSA-5689880, Ub-specific processing proteases R-HSA-68827, CDT1 association with the CDC6:ORC:origin complex R-HSA-68949, Orc1 removal from chromatin R-HSA-69017, CDK-mediated phosphorylation and removal of Cdc6 R-HSA-69481, G2/M Checkpoints R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-75815, Ubiquitin-dependent degradation of Cyclin D R-HSA-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-HSA-8932339, ROS sensing by NFE2L2 R-HSA-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-HSA-8939902, Regulation of RUNX2 expression and activity R-HSA-8941858, Regulation of RUNX3 expression and activity R-HSA-8948751, Regulation of PTEN stability and activity R-HSA-8951664, Neddylation R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9020702, Interleukin-1 signaling R-HSA-9604323, Negative regulation of NOTCH4 signaling R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q92530 |
SIGNORi | Q92530 |
Names & Taxonomyi
Protein namesi | Recommended name: Proteasome inhibitor PI31 subunitShort name: hPI31 |
Gene namesi | Name:PSMF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9571, PSMF1 |
MIMi | 617858, gene |
neXtProti | NX_Q92530 |
VEuPathDBi | HostDB:ENSG00000125818 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Nucleus
- nucleoplasm Source: Reactome
Other locations
- membrane Source: UniProtKB
- perinuclear region of cytoplasm Source: ParkinsonsUK-UCL
- proteasome core complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, ProteasomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 6 | V → R: Abolishes homodimerization. 1 Publication | 1 | |
Mutagenesisi | 83 | I → E: Abolishes interaction with FBXO7, but has no effect on homodimerization; when associated with E-90. 1 Publication | 1 | |
Mutagenesisi | 90 | I → E: Abolishes interaction with FBXO7, but has no effect on homodimerization; when associated with E-83. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9491 |
OpenTargetsi | ENSG00000125818 |
PharmGKBi | PA33917 |
Miscellaneous databases
Pharosi | Q92530, Tbio |
Genetic variation databases
BioMutai | PSMF1 |
DMDMi | 134047876 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000220920 | 2 – 271 | Proteasome inhibitor PI31 subunitAdd BLAST | 270 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 153 | PhosphoserineCombined sources | 1 | |
Modified residuei | 205 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 219 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 231 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 252 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q92530 |
jPOSTi | Q92530 |
MassIVEi | Q92530 |
MaxQBi | Q92530 |
PaxDbi | Q92530 |
PeptideAtlasi | Q92530 |
PRIDEi | Q92530 |
ProteomicsDBi | 75291 |
PTM databases
GlyGeni | Q92530, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q92530 |
PhosphoSitePlusi | Q92530 |
SwissPalmi | Q92530 |
Expressioni
Gene expression databases
Bgeei | ENSG00000125818, Expressed in testis and 250 other tissues |
ExpressionAtlasi | Q92530, baseline and differential |
Genevisiblei | Q92530, HS |
Organism-specific databases
HPAi | ENSG00000125818, Low tissue specificity |
Interactioni
Subunit structurei
Monomer and homodimer.
Interacts with FBXO7.
Interacts with the 20S proteasome.
2 PublicationsBinary interactionsi
Q92530
GO - Molecular functioni
- protein heterodimerization activity Source: ParkinsonsUK-UCL
- protein homodimerization activity Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 114872, 88 interactors |
IntActi | Q92530, 51 interactors |
MINTi | Q92530 |
STRINGi | 9606.ENSP00000338039 |
Miscellaneous databases
RNActi | Q92530, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q92530 |
SMRi | Q92530 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q92530 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 150 | Important for homodimerization and interaction with FBXO71 PublicationAdd BLAST | 149 | |
Regioni | 222 – 271 | DisorderedSequence analysisAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 249 – 263 | Pro residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the proteasome inhibitor PI31 family.Curated
Phylogenomic databases
eggNOGi | KOG4761, Eukaryota |
GeneTreei | ENSGT00390000012257 |
HOGENOMi | CLU_090116_0_0_1 |
InParanoidi | Q92530 |
OMAi | NICVEPE |
OrthoDBi | 1614691at2759 |
PhylomeDBi | Q92530 |
TreeFami | TF106238 |
Family and domain databases
InterProi | View protein in InterPro IPR045128, PI31-like IPR013886, PI31_Prot_C IPR021625, PI31_Prot_N |
PANTHERi | PTHR13266, PTHR13266, 1 hit |
Pfami | View protein in Pfam PF08577, PI31_Prot_C, 1 hit PF11566, PI31_Prot_N, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q92530-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYFGLGV GDQPGPNDKK
60 70 80 90 100
SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ
110 120 130 140 150
VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN
160 170 180 190 200
VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP
210 220 230 240 250
FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG
260 270
TSPPGPNPDH LPPPGYDDMY L
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5QPM7 | Q5QPM7_HUMAN | Proteasome inhibitor PI31 subunit | PSMF1 | 263 | Annotation score: | ||
Q5QPM9 | Q5QPM9_HUMAN | Proteasome inhibitor PI31 subunit | PSMF1 | 183 | Annotation score: | ||
H0Y555 | H0Y555_HUMAN | Proteasome inhibitor PI31 subunit | PSMF1 | 107 | Annotation score: | ||
F5H4Z3 | F5H4Z3_HUMAN | Proteasome inhibitor PI31 subunit | PSMF1 | 127 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_024564 | 36 | F → C3 PublicationsCorresponds to variant dbSNP:rs1803415Ensembl. | 1 | |
Natural variantiVAR_022153 | 174 | H → R. Corresponds to variant dbSNP:rs2235587Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88378 mRNA Translation: BAA13603.1 AL031665 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10650.1 CH471133 Genomic DNA Translation: EAX10651.1 BC126462 mRNA Translation: AAI26463.1 |
CCDSi | CCDS13010.1 |
RefSeqi | NP_001310336.1, NM_001323407.1 NP_006805.2, NM_006814.4 NP_848693.2, NM_178578.3 |
Genome annotation databases
Ensembli | ENST00000333082.7; ENSP00000327704.3; ENSG00000125818.18 ENST00000335877.11; ENSP00000338039.6; ENSG00000125818.18 |
GeneIDi | 9491 |
KEGGi | hsa:9491 |
MANE-Selecti | ENST00000335877.11; ENSP00000338039.6; NM_006814.5; NP_006805.2 |
UCSCi | uc002wel.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88378 mRNA Translation: BAA13603.1 AL031665 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10650.1 CH471133 Genomic DNA Translation: EAX10651.1 BC126462 mRNA Translation: AAI26463.1 |
CCDSi | CCDS13010.1 |
RefSeqi | NP_001310336.1, NM_001323407.1 NP_006805.2, NM_006814.4 NP_848693.2, NM_178578.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VT8 | X-ray | 2.60 | A/B | 1-151 | [»] | |
4OUH | X-ray | 2.00 | A/B/C/D | 1-158 | [»] | |
AlphaFoldDBi | Q92530 | |||||
SMRi | Q92530 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114872, 88 interactors |
IntActi | Q92530, 51 interactors |
MINTi | Q92530 |
STRINGi | 9606.ENSP00000338039 |
PTM databases
GlyGeni | Q92530, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q92530 |
PhosphoSitePlusi | Q92530 |
SwissPalmi | Q92530 |
Genetic variation databases
BioMutai | PSMF1 |
DMDMi | 134047876 |
Proteomic databases
EPDi | Q92530 |
jPOSTi | Q92530 |
MassIVEi | Q92530 |
MaxQBi | Q92530 |
PaxDbi | Q92530 |
PeptideAtlasi | Q92530 |
PRIDEi | Q92530 |
ProteomicsDBi | 75291 |
Protocols and materials databases
Antibodypediai | 23029, 278 antibodies from 36 providers |
DNASUi | 9491 |
Genome annotation databases
Ensembli | ENST00000333082.7; ENSP00000327704.3; ENSG00000125818.18 ENST00000335877.11; ENSP00000338039.6; ENSG00000125818.18 |
GeneIDi | 9491 |
KEGGi | hsa:9491 |
MANE-Selecti | ENST00000335877.11; ENSP00000338039.6; NM_006814.5; NP_006805.2 |
UCSCi | uc002wel.4, human |
Organism-specific databases
CTDi | 9491 |
DisGeNETi | 9491 |
GeneCardsi | PSMF1 |
HGNCi | HGNC:9571, PSMF1 |
HPAi | ENSG00000125818, Low tissue specificity |
MIMi | 617858, gene |
neXtProti | NX_Q92530 |
OpenTargetsi | ENSG00000125818 |
PharmGKBi | PA33917 |
VEuPathDBi | HostDB:ENSG00000125818 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4761, Eukaryota |
GeneTreei | ENSGT00390000012257 |
HOGENOMi | CLU_090116_0_0_1 |
InParanoidi | Q92530 |
OMAi | NICVEPE |
OrthoDBi | 1614691at2759 |
PhylomeDBi | Q92530 |
TreeFami | TF106238 |
Enzyme and pathway databases
PathwayCommonsi | Q92530 |
Reactomei | R-HSA-1169091, Activation of NF-kappaB in B cells R-HSA-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-HSA-1236974, ER-Phagosome pathway R-HSA-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-HSA-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174113, SCF-beta-TrCP mediated degradation of Emi1 R-HSA-174154, APC/C:Cdc20 mediated degradation of Securin R-HSA-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-180534, Vpu mediated degradation of CD4 R-HSA-180585, Vif-mediated degradation of APOBEC3G R-HSA-187577, SCF(Skp2)-mediated degradation of p27/p21 R-HSA-195253, Degradation of beta-catenin by the destruction complex R-HSA-202424, Downstream TCR signaling R-HSA-211733, Regulation of activated PAK-2p34 by proteasome mediated degradation R-HSA-2467813, Separation of Sister Chromatids R-HSA-2871837, FCERI mediated NF-kB activation R-HSA-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-HSA-350562, Regulation of ornithine decarboxylase (ODC) R-HSA-382556, ABC-family proteins mediated transport R-HSA-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-HSA-4608870, Asymmetric localization of PCP proteins R-HSA-4641257, Degradation of AXIN R-HSA-4641258, Degradation of DVL R-HSA-5358346, Hedgehog ligand biogenesis R-HSA-5362768, Hh mutants are degraded by ERAD R-HSA-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5607764, CLEC7A (Dectin-1) signaling R-HSA-5610780, Degradation of GLI1 by the proteasome R-HSA-5610783, Degradation of GLI2 by the proteasome R-HSA-5610785, GLI3 is processed to GLI3R by the proteasome R-HSA-5632684, Hedgehog 'on' state R-HSA-5658442, Regulation of RAS by GAPs R-HSA-5668541, TNFR2 non-canonical NF-kB pathway R-HSA-5676590, NIK-->noncanonical NF-kB signaling R-HSA-5678895, Defective CFTR causes cystic fibrosis R-HSA-5687128, MAPK6/MAPK4 signaling R-HSA-5689603, UCH proteinases R-HSA-5689880, Ub-specific processing proteases R-HSA-68827, CDT1 association with the CDC6:ORC:origin complex R-HSA-68949, Orc1 removal from chromatin R-HSA-69017, CDK-mediated phosphorylation and removal of Cdc6 R-HSA-69481, G2/M Checkpoints R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-75815, Ubiquitin-dependent degradation of Cyclin D R-HSA-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-HSA-8932339, ROS sensing by NFE2L2 R-HSA-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-HSA-8939902, Regulation of RUNX2 expression and activity R-HSA-8941858, Regulation of RUNX3 expression and activity R-HSA-8948751, Regulation of PTEN stability and activity R-HSA-8951664, Neddylation R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9020702, Interleukin-1 signaling R-HSA-9604323, Negative regulation of NOTCH4 signaling R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q92530 |
SIGNORi | Q92530 |
Miscellaneous databases
BioGRID-ORCSi | 9491, 95 hits in 1093 CRISPR screens |
ChiTaRSi | PSMF1, human |
EvolutionaryTracei | Q92530 |
GeneWikii | PSMF1 |
GenomeRNAii | 9491 |
Pharosi | Q92530, Tbio |
PROi | PR:Q92530 |
RNActi | Q92530, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000125818, Expressed in testis and 250 other tissues |
ExpressionAtlasi | Q92530, baseline and differential |
Genevisiblei | Q92530, HS |
Family and domain databases
InterProi | View protein in InterPro IPR045128, PI31-like IPR013886, PI31_Prot_C IPR021625, PI31_Prot_N |
PANTHERi | PTHR13266, PTHR13266, 1 hit |
Pfami | View protein in Pfam PF08577, PI31_Prot_C, 1 hit PF11566, PI31_Prot_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PSMF1_HUMAN | |
Accessioni | Q92530Primary (citable) accession number: Q92530 Secondary accession number(s): A0AVQ9, D3DVW3, Q9H4I1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | March 20, 2007 | |
Last modified: | May 25, 2022 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families