UniProtKB - Q92506 (DHB8_HUMAN)
Protein
Estradiol 17-beta-dehydrogenase 8
Gene
HSD17B8
Organism
Homo sapiens (Human)
Status
Functioni
NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (PubMed:17978863). The heterotetramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis (PubMed:19571038, PubMed:25203508). Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD (PubMed:25203508).3 Publications
Catalytic activityi
: estrogen biosynthesis Pathwayi
This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.
Pathwayi: fatty acid biosynthesis
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 156 | SubstrateBy similarity | 1 | |
Active sitei | 169 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 23 | NADCombined sources2 Publications | 9 | |
Nucleotide bindingi | 42 – 43 | NADCombined sources2 Publications | 2 | |
Nucleotide bindingi | 74 – 76 | NADCombined sources2 Publications | 3 | |
Nucleotide bindingi | 169 – 173 | NADCombined sources2 Publications | 5 | |
Nucleotide bindingi | 202 – 204 | NADCombined sources1 Publication | 3 |
GO - Molecular functioni
- 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB
- 3-oxoacyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB
- estradiol 17-beta-dehydrogenase activity Source: UniProtKB
- NADH binding Source: UniProtKB
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: GO_Central
- quinone binding Source: GO_Central
- testosterone dehydrogenase (NAD+) activity Source: UniProtKB-EC
GO - Biological processi
- androgen metabolic process Source: Ensembl
- estrogen biosynthetic process Source: UniProtKB
- fatty acid biosynthetic process Source: UniProtKB
- fatty-acyl-CoA biosynthetic process Source: Reactome
- oxidation-reduction process Source: UniProtKB
- protein heterotetramerization Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | MetaCyc:HS03575-MONOMER |
PathwayCommonsi | Q92506 |
Reactomei | R-HSA-75105, Fatty acyl-CoA biosynthesis |
SABIO-RKi | Q92506 |
UniPathwayi | UPA00094 UPA00769 |
Chemistry databases
SwissLipidsi | SLP:000001271 |
Names & Taxonomyi
Protein namesi | Recommended name: Estradiol 17-beta-dehydrogenase 8 (EC:1.1.1.621 Publication)Alternative name(s): 17-beta-hydroxysteroid dehydrogenase 81 Publication Short name: 17-beta-HSD 8 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit1 Publication Short name: KAR alpha subunit1 Publication 3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-2 Publications) Protein Ke6 Short name: Ke-6 Really interesting new gene 2 protein Short chain dehydrogenase/reductase family 30C member 1 Testosterone 17-beta-dehydrogenase 8 (EC:1.1.1.239) |
Gene namesi | Name:HSD17B8 Synonyms:FABGL, HKE6, RING2, SDR30C1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000204228.3 |
HGNCi | HGNC:3554, HSD17B8 |
MIMi | 601417, gene |
neXtProti | NX_Q92506 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Mitochondrion
- mitochondrial envelope Source: Ensembl
- mitochondrial matrix Source: UniProtKB
Plasma Membrane
- plasma membrane Source: Ensembl
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 42 | D → A: Reduced NADH-dependent reductase activity with acetoacetyl-CoA. Reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Increases NADPH-dependent reductase activities. No effect on the ability to restore growth of an OAR1-deficient yeast mutant. 1 Publication | 1 | |
Mutagenesisi | 148 | R → E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant. 1 Publication | 1 | |
Mutagenesisi | 169 | Y → A: Strongly reduced NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant. 1 Publication | 1 | |
Mutagenesisi | 173 | K → A: Abolishes NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Slightly decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant. 1 Publication | 1 | |
Mutagenesisi | 189 | R → E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 7923 |
OpenTargetsi | ENSG00000204228 |
PharmGKBi | PA29484 |
Miscellaneous databases
Pharosi | Q92506, Tbio |
Chemistry databases
DrugBanki | DB00157, NADH DB03461, Nicotinamide adenine dinucleotide phosphate |
Polymorphism and mutation databases
BioMutai | HSD17B8 |
DMDMi | 12643402 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054598 | 1 – 261 | Estradiol 17-beta-dehydrogenase 8Add BLAST | 261 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 60 | PhosphoserineBy similarity | 1 | |
Modified residuei | 160 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 173 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q92506 |
jPOSTi | Q92506 |
MassIVEi | Q92506 |
MaxQBi | Q92506 |
PaxDbi | Q92506 |
PeptideAtlasi | Q92506 |
PRIDEi | Q92506 |
ProteomicsDBi | 75276 |
TopDownProteomicsi | Q92506 |
PTM databases
iPTMneti | Q92506 |
PhosphoSitePlusi | Q92506 |
Expressioni
Tissue specificityi
Highly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed.1 Publication
Inductioni
Up-regulated by estradiol.1 Publication
Gene expression databases
Bgeei | ENSG00000204228, Expressed in right lobe of liver and 227 other tissues |
ExpressionAtlasi | Q92506, baseline and differential |
Genevisiblei | Q92506, HS |
Organism-specific databases
HPAi | ENSG00000204228, Tissue enhanced (pancreas) |
Interactioni
Subunit structurei
Heterotetramer with CBR4; contains two molecules of HSD17B8 and CBR4.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 113653, 21 interactors |
IntActi | Q92506, 16 interactors |
MINTi | Q92506 |
STRINGi | 9606.ENSP00000363794 |
Miscellaneous databases
RNActi | Q92506, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q92506 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q92506 |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | KOG1200, Eukaryota |
GeneTreei | ENSGT00940000160668 |
HOGENOMi | CLU_010194_1_3_1 |
InParanoidi | Q92506 |
OMAi | QLLMRMS |
OrthoDBi | 1226147at2759 |
PhylomeDBi | Q92506 |
TreeFami | TF313099 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q92506-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASQLQNRLR SALALVTGAG SGIGRAVSVR LAGEGATVAA CDLDRAAAQE
60 70 80 90 100
TVRLLGGPGS KEGPPRGNHA AFQADVSEAR AARCLLEQVQ ACFSRPPSVV
110 120 130 140 150
VSCAGITQDE FLLHMSEDDW DKVIAVNLKG TFLVTQAAAQ ALVSNGCRGS
160 170 180 190 200
IINISSIVGK VGNVGQTNYA ASKAGVIGLT QTAARELGRH GIRCNSVLPG
210 220 230 240 250
FIATPMTQKV PQKVVDKITE MIPMGHLGDP EDVADVVAFL ASEDSGYITG
260
TSVEVTGGLF M
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 117 | E → R in BAA11529 (PubMed:8812499).Curated | 1 | |
Sequence conflicti | 193 | R → P in BAA11529 (PubMed:8812499).Curated | 1 | |
Sequence conflicti | 208 | Q → K in BAA11529 (PubMed:8812499).Curated | 1 | |
Sequence conflicti | 212 | Q → K in BAA11529 (PubMed:8812499).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035844 | 158 | V → L in a breast cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_052316 | 190 | H → R. Corresponds to variant dbSNP:rs34491699Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT007239 mRNA Translation: AAP35903.1 AL031228 Genomic DNA Translation: CAC38444.1 AL662824 Genomic DNA No translation available. AL713971 Genomic DNA No translation available. AL645940 Genomic DNA No translation available. AL844527 Genomic DNA No translation available. CR759786 Genomic DNA No translation available. CR759733 Genomic DNA No translation available. CR847841 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03682.1 BC008185 mRNA Translation: AAH08185.1 D82061 mRNA Translation: BAA11529.1 |
CCDSi | CCDS4769.1 |
RefSeqi | NP_055049.1, NM_014234.4 |
Genome annotation databases
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT007239 mRNA Translation: AAP35903.1 AL031228 Genomic DNA Translation: CAC38444.1 AL662824 Genomic DNA No translation available. AL713971 Genomic DNA No translation available. AL645940 Genomic DNA No translation available. AL844527 Genomic DNA No translation available. CR759786 Genomic DNA No translation available. CR759733 Genomic DNA No translation available. CR847841 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03682.1 BC008185 mRNA Translation: AAH08185.1 D82061 mRNA Translation: BAA11529.1 |
CCDSi | CCDS4769.1 |
RefSeqi | NP_055049.1, NM_014234.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PD6 | X-ray | 2.00 | A/B/C/D | 6-261 | [»] | |
4CQL | X-ray | 2.85 | A/D/E/H/I/L/M/P | 1-261 | [»] | |
4CQM | X-ray | 2.34 | A/D/E/H/I/L/M/P | 1-261 | [»] | |
SMRi | Q92506 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113653, 21 interactors |
IntActi | Q92506, 16 interactors |
MINTi | Q92506 |
STRINGi | 9606.ENSP00000363794 |
Chemistry databases
DrugBanki | DB00157, NADH DB03461, Nicotinamide adenine dinucleotide phosphate |
SwissLipidsi | SLP:000001271 |
PTM databases
iPTMneti | Q92506 |
PhosphoSitePlusi | Q92506 |
Polymorphism and mutation databases
BioMutai | HSD17B8 |
DMDMi | 12643402 |
Proteomic databases
EPDi | Q92506 |
jPOSTi | Q92506 |
MassIVEi | Q92506 |
MaxQBi | Q92506 |
PaxDbi | Q92506 |
PeptideAtlasi | Q92506 |
PRIDEi | Q92506 |
ProteomicsDBi | 75276 |
TopDownProteomicsi | Q92506 |
Protocols and materials databases
Antibodypediai | 28972, 215 antibodies |
DNASUi | 7923 |
Genome annotation databases
Organism-specific databases
CTDi | 7923 |
DisGeNETi | 7923 |
EuPathDBi | HostDB:ENSG00000204228.3 |
GeneCardsi | HSD17B8 |
HGNCi | HGNC:3554, HSD17B8 |
HPAi | ENSG00000204228, Tissue enhanced (pancreas) |
MIMi | 601417, gene |
neXtProti | NX_Q92506 |
OpenTargetsi | ENSG00000204228 |
PharmGKBi | PA29484 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1200, Eukaryota |
GeneTreei | ENSGT00940000160668 |
HOGENOMi | CLU_010194_1_3_1 |
InParanoidi | Q92506 |
OMAi | QLLMRMS |
OrthoDBi | 1226147at2759 |
PhylomeDBi | Q92506 |
TreeFami | TF313099 |
Enzyme and pathway databases
UniPathwayi | UPA00094 UPA00769 |
BioCyci | MetaCyc:HS03575-MONOMER |
PathwayCommonsi | Q92506 |
Reactomei | R-HSA-75105, Fatty acyl-CoA biosynthesis |
SABIO-RKi | Q92506 |
Miscellaneous databases
BioGRID-ORCSi | 7923, 4 hits in 847 CRISPR screens |
ChiTaRSi | HSD17B8, human |
EvolutionaryTracei | Q92506 |
GeneWikii | HSD17B8 |
GenomeRNAii | 7923 |
Pharosi | Q92506, Tbio |
PROi | PR:Q92506 |
RNActi | Q92506, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204228, Expressed in right lobe of liver and 227 other tissues |
ExpressionAtlasi | Q92506, baseline and differential |
Genevisiblei | Q92506, HS |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHB8_HUMAN | |
Accessioni | Q92506Primary (citable) accession number: Q92506 Secondary accession number(s): A6NLX7, Q5STP7, Q9UIQ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 24, 2001 | |
Last modified: | December 2, 2020 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations