UniProtKB - Q924Z4 (CERS2_MOUSE)
Ceramide synthase 2
Cers2
Functioni
Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27) (PubMed:15823095, PubMed:18165233, PubMed:20110363, PubMed:23275342).
N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (PubMed:15823095, PubMed:18165233, PubMed:20110363, PubMed:23275342).
Plays a non-redundant role in the synthesis of ceramides with very-long-chain fatty acids in kidney, liver and brain. Regulates the abundance of myelin-specific sphingolipids galactosylceramide and sulfatide that affects myelin sheath architecture and motor neuron functions (PubMed:19801672, PubMed:32279995).
6 PublicationsCaution
Catalytic activityi
- a sphingoid base + a very long-chain fatty acyl-CoA = an N-(very-long-chain fatty acyl)-sphingoid base + CoA + H+3 PublicationsEC:2.3.1.2973 PublicationsThis reaction proceeds in the forward3 Publications direction.
- This reaction proceeds in the forward3 Publications direction.
- This reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forward3 Publications direction.
- This reaction proceeds in the forward1 Publication direction.
- 2-hydroxytetracosanoyl-CoA + sphinganine = CoA + H+ + N-(2-hydroxytetracosanoyl)-sphinganineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 2-hydroxytetracosenoyl-CoA + sphinganine = CoA + H+ + N-(2-hydroxytetracosenoyl)-sphinganineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- heptadecasphing-4-enine + tetracosanoyl-CoA = CoA + H+ + N-tetracosanoyl-heptadecasphing-4-enineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.3.1.241 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward2 Publications direction.
Activity regulationi
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.4 PublicationsView all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
GO - Molecular functioni
- DNA binding Source: InterPro
- N-acyltransferase activity Source: UniProtKB
- sphingosine N-acyltransferase activity Source: MGI
GO - Biological processi
- ceramide biosynthetic process Source: UniProtKB
- negative regulation of axon regeneration Source: MGI
- negative regulation of Schwann cell migration Source: MGI
- negative regulation of Schwann cell proliferation involved in axon regeneration Source: MGI
- regulation of lipid metabolic process Source: UniProtKB
- sphingolipid biosynthetic process Source: MGI
Keywordsi
Molecular function | Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.24, 3474 2.3.1.297, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
UniPathwayi | UPA00222 |
Chemistry databases
SwissLipidsi | SLP:000000116 |
Names & Taxonomyi
Protein namesi | Recommended name: Ceramide synthase 21 PublicationShort name: CerS21 Publication Alternative name(s): LAG1 longevity assurance homolog 21 Publication Sphingosine N-acyltransferase CERS2Curated (EC:2.3.1.241 Publication) Translocating chain-associating membrane protein homolog 31 Publication Short name: TRAM homolog 31 Publication Tumor metastasis-suppressor gene 1 proteinBy similarity Very-long-chain ceramide synthase CERS2Curated (EC:2.3.1.2973 Publications) |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1924143, Cers2 |
VEuPathDBi | HostDB:ENSMUSG00000015714 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 40 | Lumenal1 PublicationAdd BLAST | 40 | |
Transmembranei | 41 – 61 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 140 – 160 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 175 – 195 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 209 – 229 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 264 – 284 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 304 – 324 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 325 – 380 | CytoplasmicBy similarityAdd BLAST | 56 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185510 | 1 – 380 | Ceramide synthase 2Add BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 19 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 341 | PhosphoserineCombined sources | 1 | |
Modified residuei | 346 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 348 | PhosphoserineCombined sources | 1 | |
Modified residuei | 349 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q924Z4 |
jPOSTi | Q924Z4 |
MaxQBi | Q924Z4 |
PaxDbi | Q924Z4 |
PeptideAtlasi | Q924Z4 |
PRIDEi | Q924Z4 |
ProteomicsDBi | 281383 |
PTM databases
GlyConnecti | 2203, 2 N-Linked glycans (1 site) |
GlyGeni | Q924Z4, 1 site, 2 N-linked glycans (1 site) |
iPTMneti | Q924Z4 |
PhosphoSitePlusi | Q924Z4 |
SwissPalmi | Q924Z4 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000015714, Expressed in liver and 308 other tissues |
Genevisiblei | Q924Z4, MM |
Interactioni
Subunit structurei
Interacts with ATP6V0C, ASGR1, ASGR2 and SLC22A1/OCT1.
Interacts with ELOV1, HSD17B12 and TECR (By similarity).
Interacts with NDUFS2 (PubMed:32279995).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 218381, 50 interactors |
IntActi | Q924Z4, 31 interactors |
STRINGi | 10090.ENSMUSP00000015858 |
Miscellaneous databases
RNActi | Q924Z4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 131 – 332 | TLCPROSITE-ProRule annotationAdd BLAST | 202 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 67 – 128 | Homeobox-likeCuratedAdd BLAST | 62 | |
Regioni | 340 – 380 | DisorderedSequence analysisAdd BLAST | 41 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 291 – 300 | Last loop motifBy similarity | 10 |
Domaini
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1607, Eukaryota |
GeneTreei | ENSGT01030000234515 |
InParanoidi | Q924Z4 |
OMAi | GPMQNGH |
OrthoDBi | 987268at2759 |
PhylomeDBi | Q924Z4 |
TreeFami | TF314319 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR016439, Lag1/Lac1-like IPR006634, TLC-dom |
PANTHERi | PTHR12560, PTHR12560, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005225, LAG1_LAC1, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MLQTLYDYFW WERLWLPVNL TWADLEDKDG RVYAKASDLY ITLPLALLFL
60 70 80 90 100
VIRYFFELYV ATPLAALLNV KEKTRLRAPP NATLEHFYQT SGKQPKQVEV
110 120 130 140 150
DLLSRQSGLS GRQVERWFRR RRNQDRPSLL KKFREASWRF TYYLIAFVAG
160 170 180 190 200
MAVTVDKPWF YDLRKVWEGY PIQSIIPSQY WYYMIELSFY WSLLFSIASD
210 220 230 240 250
VKRKDFKEQI IHHVATIILL CFSWFANYVR AGTLIMALHD ASDYLLESAK
260 270 280 290 300
MFNYAGWKNT CNNLFIVFAI VFIITRLVIM PFWILHCTMI YPLELYPAFF
310 320 330 340 350
GYYFFNFMMA VLQMLHIFWA YFILRMAHKF ITGKLIEDER SDREETESSE
360 370 380
GEETAAGAGA KSRLLANGHP ILNNNHPKND
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YTM0 | D3YTM0_MOUSE | Ceramide synthase 2 | Cers2 Lass2 | 220 | Annotation score: | ||
D3Z0Z2 | D3Z0Z2_MOUSE | Ceramide synthase 2 | Cers2 Lass2 | 245 | Annotation score: | ||
D3Z4M2 | D3Z4M2_MOUSE | Ceramide synthase 2 | Cers2 Lass2 | 130 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029532 mRNA Translation: AAK40300.1 AK050161 mRNA Translation: BAC34102.1 AK159325 mRNA Translation: BAE34991.1 BC006847 mRNA Translation: AAH06847.2 |
CCDSi | CCDS17612.1 |
RefSeqi | NP_001307421.1, NM_001320492.1 NP_084065.1, NM_029789.2 XP_006502323.1, XM_006502260.1 |
Genome annotation databases
Ensembli | ENSMUST00000015858; ENSMUSP00000015858; ENSMUSG00000015714 |
GeneIDi | 76893 |
KEGGi | mmu:76893 |
UCSCi | uc008qjj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029532 mRNA Translation: AAK40300.1 AK050161 mRNA Translation: BAC34102.1 AK159325 mRNA Translation: BAE34991.1 BC006847 mRNA Translation: AAH06847.2 |
CCDSi | CCDS17612.1 |
RefSeqi | NP_001307421.1, NM_001320492.1 NP_084065.1, NM_029789.2 XP_006502323.1, XM_006502260.1 |
3D structure databases
SMRi | Q924Z4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 218381, 50 interactors |
IntActi | Q924Z4, 31 interactors |
STRINGi | 10090.ENSMUSP00000015858 |
Chemistry databases
SwissLipidsi | SLP:000000116 |
PTM databases
GlyConnecti | 2203, 2 N-Linked glycans (1 site) |
GlyGeni | Q924Z4, 1 site, 2 N-linked glycans (1 site) |
iPTMneti | Q924Z4 |
PhosphoSitePlusi | Q924Z4 |
SwissPalmi | Q924Z4 |
Proteomic databases
EPDi | Q924Z4 |
jPOSTi | Q924Z4 |
MaxQBi | Q924Z4 |
PaxDbi | Q924Z4 |
PeptideAtlasi | Q924Z4 |
PRIDEi | Q924Z4 |
ProteomicsDBi | 281383 |
Protocols and materials databases
Antibodypediai | 20302, 224 antibodies from 29 providers |
DNASUi | 76893 |
Genome annotation databases
Ensembli | ENSMUST00000015858; ENSMUSP00000015858; ENSMUSG00000015714 |
GeneIDi | 76893 |
KEGGi | mmu:76893 |
UCSCi | uc008qjj.1, mouse |
Organism-specific databases
CTDi | 29956 |
MGIi | MGI:1924143, Cers2 |
VEuPathDBi | HostDB:ENSMUSG00000015714 |
Phylogenomic databases
eggNOGi | KOG1607, Eukaryota |
GeneTreei | ENSGT01030000234515 |
InParanoidi | Q924Z4 |
OMAi | GPMQNGH |
OrthoDBi | 987268at2759 |
PhylomeDBi | Q924Z4 |
TreeFami | TF314319 |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
BRENDAi | 2.3.1.24, 3474 2.3.1.297, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 76893, 12 hits in 53 CRISPR screens |
ChiTaRSi | Cers2, mouse |
PROi | PR:Q924Z4 |
RNActi | Q924Z4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015714, Expressed in liver and 308 other tissues |
Genevisiblei | Q924Z4, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR016439, Lag1/Lac1-like IPR006634, TLC-dom |
PANTHERi | PTHR12560, PTHR12560, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005225, LAG1_LAC1, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CERS2_MOUSE | |
Accessioni | Q924Z4Primary (citable) accession number: Q924Z4 Secondary accession number(s): Q3TXC5, Q9DCN6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways