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Protein

Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Gene

Lfng

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1. Decreases the binding of JAG1 to NOTCH2 but not that of DLL1. Essential mediator of somite segmentation and patterning.By similarity

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei128SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1
Metal bindingi201ManganeseBy similarity1
Active sitei289By similarity1
Metal bindingi313ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
LigandManganese, Metal-binding

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:Lfng
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620587 Lfng

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini30 – 378LumenalSequence analysisAdd BLAST349

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191781 – 378Beta-1,3-N-acetylglucosaminyltransferase lunatic fringeAdd BLAST378

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi167 ↔ 178By similarity
Disulfide bondi196 ↔ 259By similarity
Disulfide bondi363 ↔ 372By similarity

Post-translational modificationi

A soluble form may be derived from the membrane form by proteolytic processing.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei85 – 86Cleavage; by furin-like proteaseSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ924T4
PRIDEiQ924T4

PTM databases

PhosphoSitePlusiQ924T4

Expressioni

Gene expression databases

BgeeiENSRNOG00000001250
GenevisibleiQ924T4 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001682

Structurei

3D structure databases

ProteinModelPortaliQ924T4
SMRiQ924T4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W Eukaryota
ENOG410XS8Y LUCA
GeneTreeiENSGT00390000009913
HOGENOMiHOG000046678
HOVERGENiHBG007986
InParanoidiQ924T4
KOiK05948
OMAiPHTQDVY
OrthoDBiEOG091G08XO
PhylomeDBiQ924T4
TreeFamiTF324207

Family and domain databases

InterProiView protein in InterPro
IPR017374 Fringe
IPR003378 Fringe-like
PfamiView protein in Pfam
PF02434 Fringe, 1 hit
PIRSFiPIRSF038073 B-acetylgalactosaminyltfrase, 1 hit

Sequencei

Sequence statusi: Complete.

Q924T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQRCGRRLL LALVGALLAC LLVLTADPPP TPVPAERGRR ALRSLAGSSG
60 70 80 90 100
AAPGPGSRAA VDPGVLVREV HSLSEYFSLL TRARRDADPP PGVASRQGDG
110 120 130 140 150
HPRPPAEVLS PRDVFIAVKT TRKFHRARLD LLFETWISRH KEMTFIFTDG
160 170 180 190 200
EDEALAKHTG NVVLTNCSAA HSRQALSCKM AVEYDRFIES GKKWFCHVDD
210 220 230 240 250
DNYVNLRALL RLLASYPHTQ DVYIGKPSLD RPIQATERIS EHRVRPVHFW
260 270 280 290 300
FATGGAGFCI SRGLALKMGP WASGGHFMST AERIRLPDDC TIGYIVEALL
310 320 330 340 350
GVPLIRSGLF HSHLENLQQV PTTELHEQVT LSYGMFENKR NAVHIKGPFS
360 370
VEADPSRFRS IHCHLYPDTP WCPRTAIL
Length:378
Mass (Da):41,958
Last modified:December 1, 2001 - v1
Checksum:i482A68ED60499066
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054539 mRNA Translation: BAB63256.1
BC070933 mRNA Translation: AAH70933.1
RefSeqiNP_596884.1, NM_133393.2
UniGeneiRn.127809

Genome annotation databases

EnsembliENSRNOT00000001682; ENSRNOP00000001682; ENSRNOG00000001250
GeneIDi170905
KEGGirno:170905
UCSCiRGD:620587 rat

Similar proteinsi

Entry informationi

Entry nameiLFNG_RAT
AccessioniPrimary (citable) accession number: Q924T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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