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Protein

Sprouty-related, EVH1 domain-containing protein 2

Gene

Spred2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negatively regulates Ras signaling pathways and downstream activation of MAP kinases.1 Publication

GO - Molecular functioni

  • protein kinase binding Source: BHF-UCL
  • protein serine/threonine kinase inhibitor activity Source: BHF-UCL
  • stem cell factor receptor binding Source: MGI

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5658623 FGFRL1 modulation of FGFR1 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Sprouty-related, EVH1 domain-containing protein 2
Short name:
Spred-2
Gene namesi
Name:Spred2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2150019 Spred2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000769111 – 410Sprouty-related, EVH1 domain-containing protein 2Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineBy similarity1
Modified residuei227PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues. Phosphorylated on tyrosine. Phosphorylation of Tyr-224 and Tyr-227 are required for ubiquitination.By similarity
Ubiquitinated; leading to degradation by the proteasome.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ924S7
PaxDbiQ924S7
PRIDEiQ924S7

PTM databases

iPTMnetiQ924S7
PhosphoSitePlusiQ924S7
SwissPalmiQ924S7

Expressioni

Tissue specificityi

Predominantly expressed in lung, liver and testis. In testis, it is specially found in mature spermatids projecting into the lumen of the seminiferous. Strongly expressed in glandular epithelia. Also expressed in embryonic tissues such as heart, lung, liver and brain.2 Publications

Gene expression databases

BgeeiENSMUSG00000045671 Expressed in 298 organ(s), highest expression level in cerebellum
CleanExiMM_SPRED2
ExpressionAtlasiQ924S7 baseline and differential
GenevisibleiQ924S7 MM

Interactioni

Subunit structurei

Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers (By similarity). Interacts with Ras (PubMed:11493923). May interact with ZDHHC13 (via ANK repeats) and ZDHHC17 (via ANK repeats) (PubMed:28882895).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi227830, 19 interactors
IntActiQ924S7, 18 interactors
STRINGi10090.ENSMUSP00000090987

Structurei

3D structure databases

ProteinModelPortaliQ924S7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 122WH1PROSITE-ProRule annotationAdd BLAST118
Domaini197 – 252KBDPROSITE-ProRule annotationAdd BLAST56
Domaini300 – 408SPRPROSITE-ProRule annotationAdd BLAST109

Phylogenomic databases

eggNOGiENOG410IQ21 Eukaryota
ENOG410Y62R LUCA
GeneTreeiENSGT00730000110272
HOGENOMiHOG000220886
HOVERGENiHBG057556
InParanoidiQ924S7
KOiK04703

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR023337 KBD
IPR011993 PH-like_dom_sf
IPR007875 Sprouty
IPR000697 WH1/EVH1_dom
PfamiView protein in Pfam
PF05210 Sprouty, 1 hit
PF00568 WH1, 1 hit
SMARTiView protein in SMART
SM00461 WH1, 1 hit
PROSITEiView protein in PROSITE
PS51488 KBD, 1 hit
PS51227 SPR, 1 hit
PS50229 WH1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q924S7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEETHPDDD SYIVRVKAVV MTRDDSSGGW FPQEGGGISR VGVCKVMHPE
60 70 80 90 100
GNGRSGFLIH GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL
110 120 130 140 150
TFQSPADARA FDRGVRKAIE DLIEGSTTSS STLHNEAELG DDDVFTTATD
160 170 180 190 200
SSSNSSQKRE PTTRTISSPT SCEHRKIYTL DPYPMDHYHP DQRLPRSYPQ
210 220 230 240 250
VTFPEDDEEI VRINPREKIW MTGYEDYRHA PVRGKYLDTT EDADSYVRFA
260 270 280 290 300
KGEVPKHEYT YPYVDSSDFG FGEDPKGSVI KTQPPRAKSR RRKENGERSR
310 320 330 340 350
CVYCRDMFNH EENRRGHCQD APDAVRTCIR RVSCMWCADS MLYHCMSDPE
360 370 380 390 400
GDYTDPCSCD TSDEKFCLRW MALIALSFLA PCMCCYLPLR ACHRCGVMCR
410
CCGGKHKAAA
Length:410
Mass (Da):46,794
Last modified:December 1, 2001 - v1
Checksum:i8A652D06CE8D3BB1
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5SRF8Q5SRF8_MOUSE
Sprouty-related, EVH1 domain-contai...
Spred2
437Annotation score:
Q5SRF9Q5SRF9_MOUSE
Sprouty-related, EVH1 domain-contai...
Spred2
229Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB063496 mRNA Translation: BAB62849.1
AK036430 mRNA Translation: BAC29425.1
BC066013 mRNA Translation: AAH66013.1
CCDSiCCDS24454.1
RefSeqiNP_277058.1, NM_033523.4
UniGeneiMm.266627
Mm.445184

Genome annotation databases

EnsembliENSMUST00000093298; ENSMUSP00000090987; ENSMUSG00000045671
GeneIDi114716
KEGGimmu:114716
UCSCiuc007icr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB063496 mRNA Translation: BAB62849.1
AK036430 mRNA Translation: BAC29425.1
BC066013 mRNA Translation: AAH66013.1
CCDSiCCDS24454.1
RefSeqiNP_277058.1, NM_033523.4
UniGeneiMm.266627
Mm.445184

3D structure databases

ProteinModelPortaliQ924S7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi227830, 19 interactors
IntActiQ924S7, 18 interactors
STRINGi10090.ENSMUSP00000090987

PTM databases

iPTMnetiQ924S7
PhosphoSitePlusiQ924S7
SwissPalmiQ924S7

Proteomic databases

MaxQBiQ924S7
PaxDbiQ924S7
PRIDEiQ924S7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093298; ENSMUSP00000090987; ENSMUSG00000045671
GeneIDi114716
KEGGimmu:114716
UCSCiuc007icr.1 mouse

Organism-specific databases

CTDi200734
MGIiMGI:2150019 Spred2

Phylogenomic databases

eggNOGiENOG410IQ21 Eukaryota
ENOG410Y62R LUCA
GeneTreeiENSGT00730000110272
HOGENOMiHOG000220886
HOVERGENiHBG057556
InParanoidiQ924S7
KOiK04703

Enzyme and pathway databases

ReactomeiR-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5658623 FGFRL1 modulation of FGFR1 signaling

Miscellaneous databases

ChiTaRSiSpred2 mouse
PROiPR:Q924S7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045671 Expressed in 298 organ(s), highest expression level in cerebellum
CleanExiMM_SPRED2
ExpressionAtlasiQ924S7 baseline and differential
GenevisibleiQ924S7 MM

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR023337 KBD
IPR011993 PH-like_dom_sf
IPR007875 Sprouty
IPR000697 WH1/EVH1_dom
PfamiView protein in Pfam
PF05210 Sprouty, 1 hit
PF00568 WH1, 1 hit
SMARTiView protein in SMART
SM00461 WH1, 1 hit
PROSITEiView protein in PROSITE
PS51488 KBD, 1 hit
PS51227 SPR, 1 hit
PS50229 WH1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPRE2_MOUSE
AccessioniPrimary (citable) accession number: Q924S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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