UniProtKB - Q924S5 (LONM_RAT)
Protein
Lon protease homolog, mitochondrial
Gene
Lonp1
Organism
Rattus norvegicus (Rat)
Status
Functioni
ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters.UniRule annotation2 Publications
Catalytic activityi
- Hydrolysis of proteins in presence of ATP.UniRule annotation EC:3.4.21.53
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 845 | UniRule annotation | 1 | |
Active sitei | 888 | UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 513 – 520 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ADP binding Source: RGD
- ATPase activity Source: RGD
- ATP binding Source: RGD
- ATP-dependent peptidase activity Source: RGD
- DNA polymerase binding Source: RGD
- G-quadruplex DNA binding Source: RGD
- identical protein binding Source: RGD
- mitochondrial promoter sequence-specific DNA binding Source: RGD
- sequence-specific DNA binding Source: RGD
- serine-type endopeptidase activity Source: UniProtKB-UniRule
- single-stranded DNA binding Source: RGD
- single-stranded RNA binding Source: RGD
GO - Biological processi
- aging Source: RGD
- cellular protein-containing complex assembly Source: RGD
- cellular response to oxidative stress Source: RGD
- chaperone-mediated protein complex assembly Source: GO_Central
- mitochondrion organization Source: RGD
- oxidation-dependent protein catabolic process Source: RGD
- protein quality control for misfolded or incompletely synthesized proteins Source: GO_Central
- proteolysis Source: RGD
- proteolysis involved in cellular protein catabolic process Source: RGD
- response to aluminum ion Source: RGD
- response to hormone Source: RGD
- response to hypoxia Source: RGD
Keywordsi
Molecular function | DNA-binding, Hydrolase, Protease, Serine protease |
Ligand | ATP-binding, Nucleotide-binding |
Protein family/group databases
MEROPSi | S16.002 |
Names & Taxonomyi
Protein namesi | Recommended name: Lon protease homolog, mitochondrialUniRule annotation (EC:3.4.21.53UniRule annotation)Alternative name(s): Lon protease-like proteinUniRule annotation Short name: LONPUniRule annotation Mitochondrial ATP-dependent protease LonUniRule annotation Serine protease 15UniRule annotation |
Gene namesi | Name:Lonp1 Synonyms:Lon, Prss15 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621598, Lonp1 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix UniRule annotation3 Publications
Cytosol
- cytosol Source: Ensembl
Mitochondrion
- mitochondrial matrix Source: RGD
- mitochondrial nucleoid Source: RGD
- mitochondrion Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 65 | MitochondrionUniRule annotationAdd BLAST | 65 | |
ChainiPRO_0000254962 | 66 – 950 | Lon protease homolog, mitochondrialAdd BLAST | 885 |
Proteomic databases
jPOSTi | Q924S5 |
PaxDbi | Q924S5 |
PRIDEi | Q924S5 |
PTM databases
iPTMneti | Q924S5 |
PhosphoSitePlusi | Q924S5 |
Expressioni
Inductioni
By hypoxia or ER stress.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000046502, Expressed in brain and 21 other tissues |
Genevisiblei | Q924S5, RN |
Interactioni
Subunit structurei
Homohexamer. Organized in a ring with a central cavity. The ATP-binding and proteolytic domains (AP-domain) form a hexameric chamber, while the N-terminal domain is arranged as a trimer of dimers. DNA and RNA binding is stimulated by substrate and inhibited by ATP binding.
Interacts with TWNK and mitochondrial DNA polymerase subunit POLG.
UniRule annotationGO - Molecular functioni
- DNA polymerase binding Source: RGD
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251027, 1 interactor |
IntActi | Q924S5, 2 interactors |
STRINGi | 10116.ENSRNOP00000066618 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 112 – 358 | Lon N-terminalPROSITE-ProRule annotationAdd BLAST | 247 | |
Domaini | 749 – 939 | Lon proteolyticPROSITE-ProRule annotationAdd BLAST | 191 |
Sequence similaritiesi
Belongs to the peptidase S16 family.UniRule annotation
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2004, Eukaryota |
GeneTreei | ENSGT00530000063553 |
HOGENOMi | CLU_004109_1_1_1 |
InParanoidi | Q924S5 |
OMAi | ENVAHED |
OrthoDBi | 528132at2759 |
PhylomeDBi | Q924S5 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit |
HAMAPi | MF_03120, lonm_euk, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR004815, Lon_bac/euk-typ IPR008269, Lon_proteolytic IPR027065, Lon_Prtase IPR003111, Lon_substr-bd IPR027503, Lonm_euk IPR027417, P-loop_NTPase IPR008268, Peptidase_S16_AS IPR015947, PUA-like_sf IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
PANTHERi | PTHR43718, PTHR43718, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF05362, Lon_C, 1 hit PF02190, LON_substr_bdg, 1 hit |
PIRSFi | PIRSF001174, Lon_proteas, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00464, LON, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00763, lon, 1 hit |
PROSITEi | View protein in PROSITE PS51787, LON_N, 1 hit PS51786, LON_PROTEOLYTIC, 1 hit PS01046, LON_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q924S5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAASTGYVRL WAAARCWVLR RPLLAVTGGR VPSASGSWLR RGCRVCDTST
60 70 80 90 100
PWGGRVPMGG GQWRGLWDAG SRGGSDETSE GGVEDGATAS SGEGPVVTAL
110 120 130 140 150
APMTVPDVFP HLPLIAISRN PVFPRFIKIV EVKNKKLVEL LRRKVRLAQP
160 170 180 190 200
YVGVFLKRDD NNESDVVESL DEIYHTGTFA QIHEMQDLGD KLRMIVTGHR
210 220 230 240 250
RIHISRQLEV EPEGLEPEAE NKQKSRRKLK RGKKEVGDEL GAKPQLEMVT
260 270 280 290 300
EATSDTSKEV LMVEVENVAH EDFQVTEEVK ALTAEIVKTI RDIIALNPLY
310 320 330 340 350
RESVLQMMQA GQRVVDNPIY LSDMGAALTG AESHELQDVL EETNILKRLY
360 370 380 390 400
KALSLLKKEF ELSKLQQRLG REVEEKIKQT HRKYLLQEQL KIIKKELGLE
410 420 430 440 450
KDDKDAIEEK FRERLKELVV PKHVMDVVDE ELSKLALLDN HSSEFNVTRN
460 470 480 490 500
YLDWLTSIPW GRQSDENLDL ARAQSVLEED HYGMEDVKKR VLEFIAVSQL
510 520 530 540 550
RGSTQGKILC FHGPPGVGKT SIARSIARAL GREYFRFSVG GMTDVAEIKG
560 570 580 590 600
HRRTYVGAMP GKIIQCLKKT KTENPLVLID EVDKIGRGYQ GDPSSALLEL
610 620 630 640 650
LDPEQNANFL DHYLDVPVDL SKVLFICTAN VTDTIPEPLR DRMEMINVSG
660 670 680 690 700
YVAQEKLAIA ERYLVPQART LCGLDESKAQ LSATVLTLLI KQYCRESGVR
710 720 730 740 750
NLQKQVEKVL RKAAYKIVSG EAQTVHVTPE NLQDFVGKPV FTVERMYDVT
760 770 780 790 800
PPGVVMGLAW TAMGGSTLFV ETSLRRPQPS GSKEDKDGSL EVTGQLGDVM
810 820 830 840 850
KESARIAYTF ARAFLMEQDP ENDFLVTSHI HLHVPEGATP KDGPSAGCTI
860 870 880 890 900
VTALLSLALG QPVLQNLAMT GEVSLTGKVL PVGGIKEKTI AAKRAGVTCI
910 920 930 940 950
ILPAENRKDF SDLAPFITEG LEVHFVEHYR DIFRIAFPLR EHQEALAVER
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB064323 mRNA Translation: BAB62423.1 |
RefSeqi | NP_596895.1, NM_133404.1 |
Genome annotation databases
Ensembli | ENSRNOT00000074253; ENSRNOP00000066618; ENSRNOG00000046502 |
GeneIDi | 170916 |
KEGGi | rno:170916 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB064323 mRNA Translation: BAB62423.1 |
RefSeqi | NP_596895.1, NM_133404.1 |
3D structure databases
SMRi | Q924S5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 251027, 1 interactor |
IntActi | Q924S5, 2 interactors |
STRINGi | 10116.ENSRNOP00000066618 |
Protein family/group databases
MEROPSi | S16.002 |
PTM databases
iPTMneti | Q924S5 |
PhosphoSitePlusi | Q924S5 |
Proteomic databases
jPOSTi | Q924S5 |
PaxDbi | Q924S5 |
PRIDEi | Q924S5 |
Genome annotation databases
Ensembli | ENSRNOT00000074253; ENSRNOP00000066618; ENSRNOG00000046502 |
GeneIDi | 170916 |
KEGGi | rno:170916 |
Organism-specific databases
CTDi | 9361 |
RGDi | 621598, Lonp1 |
Phylogenomic databases
eggNOGi | KOG2004, Eukaryota |
GeneTreei | ENSGT00530000063553 |
HOGENOMi | CLU_004109_1_1_1 |
InParanoidi | Q924S5 |
OMAi | ENVAHED |
OrthoDBi | 528132at2759 |
PhylomeDBi | Q924S5 |
Miscellaneous databases
PROi | PR:Q924S5 |
Gene expression databases
Bgeei | ENSRNOG00000046502, Expressed in brain and 21 other tissues |
Genevisiblei | Q924S5, RN |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit |
HAMAPi | MF_03120, lonm_euk, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR004815, Lon_bac/euk-typ IPR008269, Lon_proteolytic IPR027065, Lon_Prtase IPR003111, Lon_substr-bd IPR027503, Lonm_euk IPR027417, P-loop_NTPase IPR008268, Peptidase_S16_AS IPR015947, PUA-like_sf IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
PANTHERi | PTHR43718, PTHR43718, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF05362, Lon_C, 1 hit PF02190, LON_substr_bdg, 1 hit |
PIRSFi | PIRSF001174, Lon_proteas, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00464, LON, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00763, lon, 1 hit |
PROSITEi | View protein in PROSITE PS51787, LON_N, 1 hit PS51786, LON_PROTEOLYTIC, 1 hit PS01046, LON_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LONM_RAT | |
Accessioni | Q924S5Primary (citable) accession number: Q924S5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries