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Protein

Mannose-6-phosphate isomerase

Gene

Mpi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (Mpi), Mannose-6-phosphate isomerase (Mpi), Mannose-6-phosphate isomerase (Mpi)
  2. Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm2), Phosphomannomutase 2 (Pmm2), Phosphomannomutase 1 (Pmm1), Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm1)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110ZincBy similarity1
Metal bindingi112ZincBy similarity1
Metal bindingi137ZincBy similarity1
Metal bindingi276ZincBy similarity1
Active sitei295By similarity1

GO - Molecular functioni

GO - Biological processi

  • cell wall mannoprotein biosynthetic process Source: GO_Central
  • GDP-mannose biosynthetic process Source: MGI
  • glycolytic process from mannose through fructose-6-phosphate Source: MGI
  • mannose to fructose-6-phosphate metabolic process Source: MGI
  • protein glycosylation Source: GO_Central

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-446205 Synthesis of GDP-mannose
UniPathwayiUPA00126; UER00423

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:Mpi
Synonyms:Mpi1, Pmi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:97075 Mpi

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001942372 – 423Mannose-6-phosphate isomeraseAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Modified residuei108PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ924M7
MaxQBiQ924M7
PaxDbiQ924M7
PRIDEiQ924M7

2D gel databases

REPRODUCTION-2DPAGEiQ924M7

PTM databases

iPTMnetiQ924M7
PhosphoSitePlusiQ924M7

Expressioni

Gene expression databases

BgeeiENSMUSG00000032306
CleanExiMM_MPI
ExpressionAtlasiQ924M7 baseline and differential
GenevisibleiQ924M7 MM

Interactioni

Protein-protein interaction databases

IntActiQ924M7, 2 interactors
MINTiQ924M7
STRINGi10090.ENSMUSP00000034856

Structurei

3D structure databases

ProteinModelPortaliQ924M7
SMRiQ924M7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2757 Eukaryota
COG1482 LUCA
GeneTreeiENSGT00390000016075
HOGENOMiHOG000241277
HOVERGENiHBG000367
InParanoidiQ924M7
KOiK01809
OMAiMGTHPSN
OrthoDBiEOG091G078D
PhylomeDBiQ924M7
TreeFamiTF312831

Family and domain databases

Gene3Di2.60.120.10, 4 hits
InterProiView protein in InterPro
IPR001250 Man6P_Isoase-1
IPR016305 Mannose-6-P_Isomerase
IPR018050 Pmannose_isomerase-type1_CS
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR10309 PTHR10309, 1 hit
PfamiView protein in Pfam
PF01238 PMI_typeI, 1 hit
PIRSFiPIRSF001480 Mannose-6-phosphate_isomerase, 1 hit
PRINTSiPR00714 MAN6PISMRASE
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR00218 manA, 1 hit
PROSITEiView protein in PROSITE
PS00965 PMI_I_1, 1 hit
PS00966 PMI_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q924M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPRVFPLS CVVQQYAWGK VGSKSEVACL LASSDPLAQI SEDKPYAELW
60 70 80 90 100
MGTHPRGDAK ILDNRISQKT LGQWIAENPD CLGSKVKNTF NGKLPFLFKV
110 120 130 140 150
LSVDTALSIQ AHPNKELAEK LHLQAPEHYP DANHKPEMAI ALTSFQGLCG
160 170 180 190 200
FRPVEEIVTF MKKVPEFQLL IGDDATAQLK ESVGGDTEAM ASALRNCFSH
210 220 230 240 250
LMKSEKKVVV EQLNLLVKRI SQQVFDGNNM EDIYGKLLLQ LHQQHPGDIG
260 270 280 290 300
CFAIYFLNLL TLKPGEAMFL DANVPHAYLK GDCVECMACS DNTVRAGLTP
310 320 330 340 350
KFIDVPTLCE MLNYTPSPSN NRLFAPAQSQ DDPYLSIYDP PVPDFTVMKM
360 370 380 390 400
EVPSSVTEYK VSTLDSASIL LMVQGTVTAI IPSAHAEIPL YRGGVLFIAA
410 420
NESVLLKITV PKDLLIFRAC CLL
Length:423
Mass (Da):46,575
Last modified:December 1, 2001 - v1
Checksum:i3A49506D1249B670
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF244360 mRNA Translation: AAK69388.1
BC051163 mRNA Translation: AAH51163.1
CCDSiCCDS40650.1
RefSeqiNP_080113.1, NM_025837.2
UniGeneiMm.247218

Genome annotation databases

EnsembliENSMUST00000034856; ENSMUSP00000034856; ENSMUSG00000032306
GeneIDi110119
KEGGimmu:110119
UCSCiuc009pvd.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMPI_MOUSE
AccessioniPrimary (citable) accession number: Q924M7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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