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Entry version 196 (07 Oct 2020)
Sequence version 2 (01 Aug 1998)
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Protein

ATP-dependent RNA helicase DDX1

Gene

DDX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed:24870230). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (By similarity).By similarity6 Publications
(Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs.1 Publication
(Microbial infection) Required for Coronavirus IBV replication.1 Publication

Caution

According to some authors the unwinding activity is ADP-dependent and not ATP-dependent.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 1.9 min(-1) for ATP hydrolysis in the absence of nucleic acid (PubMed:21589879). kcat is 3.8 min(-1) for ATP hydrolysis in the presence of RNA oligo(rU)20 (PubMed:21589879). kcat is 2.1 min(-1) for ATP hydrolysis in the presence of DNA oligo(dT)20 (PubMed:21589879).1 Publication
  1. KM=0.120 mM for ATP (in the absence of nucleic acid)1 Publication
  2. KM=0.090 mM for ATP (in the presence of RNA oligo(rU)20)1 Publication
  3. KM=0.095 mM for ATP (in the presence of DNA oligo(dT)20)1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi46 – 53ATPCurated8

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionActivator, DNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease, RNA-binding
    Biological processAntiviral defense, Host-virus interaction, Immunity, Innate immunity, mRNA processing, Transcription, Transcription regulation, tRNA processing
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:ENSG00000079785-MONOMER

    Pathway Commons web resource for biological pathway data

    More...
    PathwayCommonsi
    Q92499

    Reactome - a knowledgebase of biological pathways and processes

    More...
    Reactomei
    R-HSA-6784531, tRNA processing in the nucleus

    SIGNOR Signaling Network Open Resource

    More...
    SIGNORi
    Q92499

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    ATP-dependent RNA helicase DDX1 (EC:3.6.4.131 Publication)
    Alternative name(s):
    DEAD box protein 1
    DEAD box protein retinoblastoma
    Short name:
    DBP-RB
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:DDX1
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

    Organism-specific databases

    Eukaryotic Pathogen Database Resources

    More...
    EuPathDBi
    HostDB:ENSG00000079785.14

    Human Gene Nomenclature Database

    More...
    HGNCi
    HGNC:2734, DDX1

    Online Mendelian Inheritance in Man (OMIM)

    More...
    MIMi
    601257, gene

    neXtProt; the human protein knowledge platform

    More...
    neXtProti
    NX_Q92499

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi52K → N: Abolishes ability to promote guanylylation of RTCB. 1 Publication1
    Mutagenesisi371E → G: Inhibits the transcriptional activity of RELA and attenuates NF-kappa-B-mediated gene expression. 2 Publications1
    Mutagenesisi371E → Q: Abolishes ability to promote guanylylation of RTCB. 2 Publications1

    Organism-specific databases

    DisGeNET

    More...
    DisGeNETi
    1653

    Open Targets

    More...
    OpenTargetsi
    ENSG00000079785

    The Pharmacogenetics and Pharmacogenomics Knowledge Base

    More...
    PharmGKBi
    PA27199

    Miscellaneous databases

    Pharos NIH Druggable Genome Knowledgebase

    More...
    Pharosi
    Q92499, Tbio

    Chemistry databases

    ChEMBL database of bioactive drug-like small molecules

    More...
    ChEMBLi
    CHEMBL2010634

    Polymorphism and mutation databases

    BioMuta curated single-nucleotide variation and disease association database

    More...
    BioMutai
    DDX1

    Domain mapping of disease mutations (DMDM)

    More...
    DMDMi
    6919862

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000549861 – 740ATP-dependent RNA helicase DDX1Add BLAST740

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei239N6-acetyllysineCombined sources1
    Modified residuei268N6-acetyllysineCombined sources1
    Modified residuei281N6-acetyllysine; alternateCombined sources1
    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
    Modified residuei481PhosphoserineCombined sources1

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    The CPTAC Assay portal

    More...
    CPTACi
    CPTAC-190
    CPTAC-191

    Encyclopedia of Proteome Dynamics

    More...
    EPDi
    Q92499

    jPOST - Japan Proteome Standard Repository/Database

    More...
    jPOSTi
    Q92499

    MassIVE - Mass Spectrometry Interactive Virtual Environment

    More...
    MassIVEi
    Q92499

    MaxQB - The MaxQuant DataBase

    More...
    MaxQBi
    Q92499

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    Q92499

    PeptideAtlas

    More...
    PeptideAtlasi
    Q92499

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q92499

    ProteomicsDB: a multi-organism proteome resource

    More...
    ProteomicsDBi
    4603
    4798
    75269 [Q92499-1]

    2D gel databases

    REPRODUCTION-2DPAGE

    More...
    REPRODUCTION-2DPAGEi
    IPI00293655

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    Q92499

    Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

    More...
    PhosphoSitePlusi
    Q92499

    SwissPalm database of S-palmitoylation events

    More...
    SwissPalmi
    Q92499

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.1 Publication

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...
    Bgeei
    ENSG00000079785, Expressed in tendon and 247 other tissues

    ExpressionAtlas, Differential and Baseline Expression

    More...
    ExpressionAtlasi
    Q92499, baseline and differential

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...
    Genevisiblei
    Q92499, HS

    Organism-specific databases

    Human Protein Atlas

    More...
    HPAi
    ENSG00000079785, Low tissue specificity

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    (Microbial infection) Interacts with Venezuelan equine encephalitis virus non-structural protein 3.

    1 Publication

    Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1; this complex exists in resting cells with or without poly(I:C) RNA ligand stimulation.

    Interacts with DHX36.

    Interacts (via B30.2/SPRY domain) with DDX21 (via N-terminus); this interaction serves as bridges to TICAM1 (By similarity).

    Interacts with FAM98A (via N- and C-terminus) (PubMed:28040436).

    Interacts with MBNL1 (PubMed:18335541).

    Interacts with CSTF2 (PubMed:11598190).

    Interacts with HNRNPK (PubMed:12183465).

    Interacts with ATM (PubMed:18710941).

    Interacts with RELA (via C-terminus) (PubMed:19058135).

    Component of the tRNA-splicing ligase complex (PubMed:21311021, PubMed:24870230).

    Interacts with PQBP1 (PubMed:21933836).

    Interacts with PHF5A (via C-terminus) (By similarity).

    Interacts with ERCC6 (PubMed:26030138).

    By similarity10 Publications

    (Microbial infection) Interacts with Rev of HIV-1.

    1 Publication

    (Microbial infection) Interacts with Severe acute respiratory syndrome coronavirus (SARS-CoV) (via N-terminus) (PubMed:20573827).

    Interacts (via C-terminus) with the replicase polyprotein 1ab Nsp14 of the Avian infectious bronchitis virus (IBV).

    1 Publication

    <p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    108019, 283 interactors

    CORUM comprehensive resource of mammalian protein complexes

    More...
    CORUMi
    Q92499

    Database of interacting proteins

    More...
    DIPi
    DIP-38163N

    Protein interaction database and analysis system

    More...
    IntActi
    Q92499, 68 interactors

    Molecular INTeraction database

    More...
    MINTi
    Q92499

    STRING: functional protein association networks

    More...
    STRINGi
    9606.ENSP00000370745

    Chemistry databases

    BindingDB database of measured binding affinities

    More...
    BindingDBi
    Q92499

    Miscellaneous databases

    RNAct, Protein-RNA interaction predictions for model organisms.

    More...
    RNActi
    Q92499, protein

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1740
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q92499

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    Protein Data Bank in Europe - Knowledge Base

    More...
    PDBe-KBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 428Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST427
    Domaini70 – 247B30.2/SPRYPROSITE-ProRule annotationAdd BLAST178
    Domaini493 – 681Helicase C-terminalPROSITE-ProRule annotationAdd BLAST189

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 525Necessary for interaction with RELA1 PublicationAdd BLAST525
    Regioni1 – 448Interaction with dsRNABy similarityAdd BLAST448
    Regioni1 – 295Necessary for interaction with HNRNPK1 PublicationAdd BLAST295
    Regioni525 – 740Necessary for interaction with HNRNPK1 PublicationAdd BLAST216
    Regioni536 – 631Necessary for interaction with replicase polyprotein 1ab nsp14 of IBV1 PublicationAdd BLAST96

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi370 – 373DEAD boxPROSITE-ProRule annotation4

    <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    The helicase domain is involved in the stimulation of RELA transcriptional activity.1 Publication

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG0349, Eukaryota

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00940000155678

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_016321_0_0_1

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q92499

    KEGG Orthology (KO)

    More...
    KOi
    K13177

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    DGVHDRD

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    Q92499

    TreeFam database of animal gene trees

    More...
    TreeFami
    TF106114

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    2.60.120.920, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR001870, B30.2/SPRY
    IPR043136, B30.2/SPRY_sf
    IPR013320, ConA-like_dom_sf
    IPR011545, DEAD/DEAH_box_helicase_dom
    IPR014001, Helicase_ATP-bd
    IPR001650, Helicase_C
    IPR027417, P-loop_NTPase
    IPR014014, RNA_helicase_DEAD_Q_motif
    IPR003877, SPRY_dom

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00270, DEAD, 1 hit
    PF00271, Helicase_C, 1 hit
    PF00622, SPRY, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00487, DEXDc, 1 hit
    SM00490, HELICc, 1 hit
    SM00449, SPRY, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF49899, SSF49899, 1 hit
    SSF52540, SSF52540, 2 hits

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS50188, B302_SPRY, 1 hit
    PS51192, HELICASE_ATP_BIND_1, 2 hits
    PS51194, HELICASE_CTER, 1 hit
    PS51195, Q_MOTIF, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q92499-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MAAFSEMGVM PEIAQAVEEM DWLLPTDIQA ESIPLILGGG DVLMAAETGS
    60 70 80 90 100
    GKTGAFSIPV IQIVYETLKD QQEGKKGKTT IKTGASVLNK WQMNPYDRGS
    110 120 130 140 150
    AFAIGSDGLC CQSREVKEWH GCRATKGLMK GKHYYEVSCH DQGLCRVGWS
    160 170 180 190 200
    TMQASLDLGT DKFGFGFGGT GKKSHNKQFD NYGEEFTMHD TIGCYLDIDK
    210 220 230 240 250
    GHVKFSKNGK DLGLAFEIPP HMKNQALFPA CVLKNAELKF NFGEEEFKFP
    260 270 280 290 300
    PKDGFVALSK APDGYIVKSQ HSGNAQVTQT KFLPNAPKAL IVEPSRELAE
    310 320 330 340 350
    QTLNNIKQFK KYIDNPKLRE LLIIGGVAAR DQLSVLENGV DIVVGTPGRL
    360 370 380 390 400
    DDLVSTGKLN LSQVRFLVLD EADGLLSQGY SDFINRMHNQ IPQVTSDGKR
    410 420 430 440 450
    LQVIVCSATL HSFDVKKLSE KIMHFPTWVD LKGEDSVPDT VHHVVVPVNP
    460 470 480 490 500
    KTDRLWERLG KSHIRTDDVH AKDNTRPGAN SPEMWSEAIK ILKGEYAVRA
    510 520 530 540 550
    IKEHKMDQAI IFCRTKIDCD NLEQYFIQQG GGPDKKGHQF SCVCLHGDRK
    560 570 580 590 600
    PHERKQNLER FKKGDVRFLI CTDVAARGID IHGVPYVINV TLPDEKQNYV
    610 620 630 640 650
    HRIGRVGRAE RMGLAISLVA TEKEKVWYHV CSSRGKGCYN TRLKEDGGCT
    660 670 680 690 700
    IWYNEMQLLS EIEEHLNCTI SQVEPDIKVP VDEFDGKVTY GQKRAAGGGS
    710 720 730 740
    YKGHVDILAP TVQELAALEK EAQTSFLHLG YLPNQLFRTF
    Length:740
    Mass (Da):82,432
    Last modified:August 1, 1998 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC6D0179F83BD8C73
    GO
    Isoform 2 (identifier: Q92499-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         98-113: Missing.
         630-740: VCSSRGKGCY...YLPNQLFRTF → MVQRDAVTI

    Show »
    Length:622
    Mass (Da):69,556
    Checksum:iF0DC36DB1DAA09F8
    GO
    Isoform 3 (identifier: Q92499-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-128: Missing.

    Show »
    Length:612
    Mass (Da):68,712
    Checksum:i511B121802355812
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    F1T0B3F1T0B3_HUMAN
    ATP-dependent RNA helicase DDX1
    DDX1
    659Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A087X2G1A0A087X2G1_HUMAN
    ATP-dependent RNA helicase DDX1
    DDX1
    659Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    C9JLP0C9JLP0_HUMAN
    ATP-dependent RNA helicase DDX1
    DDX1
    16Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0554531 – 128Missing in isoform 3. 1 PublicationAdd BLAST128
    Alternative sequenceiVSP_05545498 – 113Missing in isoform 2. 1 PublicationAdd BLAST16
    Alternative sequenceiVSP_055455630 – 740VCSSR…LFRTF → MVQRDAVTI in isoform 2. 1 PublicationAdd BLAST111

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    X70649 mRNA Translation: CAA49992.1
    AK297432 mRNA Translation: BAG59860.1
    AK298426 mRNA Translation: BAG60648.1
    BC012132 mRNA Translation: AAH12132.1
    BC053673 mRNA Translation: AAH53673.1

    The Consensus CDS (CCDS) project

    More...
    CCDSi
    CCDS1686.1 [Q92499-1]

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_004930.1, NM_004939.2 [Q92499-1]

    Genome annotation databases

    Ensembl eukaryotic genome annotation project

    More...
    Ensembli
    ENST00000233084; ENSP00000233084; ENSG00000079785 [Q92499-1]
    ENST00000381341; ENSP00000370745; ENSG00000079785 [Q92499-1]

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    1653

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    hsa:1653

    UCSC genome browser

    More...
    UCSCi
    uc002rce.5, human [Q92499-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    <p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X70649 mRNA Translation: CAA49992.1
    AK297432 mRNA Translation: BAG59860.1
    AK298426 mRNA Translation: BAG60648.1
    BC012132 mRNA Translation: AAH12132.1
    BC053673 mRNA Translation: AAH53673.1
    CCDSiCCDS1686.1 [Q92499-1]
    RefSeqiNP_004930.1, NM_004939.2 [Q92499-1]

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4XW3X-ray2.00A/B72-283[»]
    SMRiQ92499
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    BioGRIDi108019, 283 interactors
    CORUMiQ92499
    DIPiDIP-38163N
    IntActiQ92499, 68 interactors
    MINTiQ92499
    STRINGi9606.ENSP00000370745

    Chemistry databases

    BindingDBiQ92499
    ChEMBLiCHEMBL2010634

    PTM databases

    iPTMnetiQ92499
    PhosphoSitePlusiQ92499
    SwissPalmiQ92499

    Polymorphism and mutation databases

    BioMutaiDDX1
    DMDMi6919862

    2D gel databases

    REPRODUCTION-2DPAGEiIPI00293655

    Proteomic databases

    CPTACiCPTAC-190
    CPTAC-191
    EPDiQ92499
    jPOSTiQ92499
    MassIVEiQ92499
    MaxQBiQ92499
    PaxDbiQ92499
    PeptideAtlasiQ92499
    PRIDEiQ92499
    ProteomicsDBi4603
    4798
    75269 [Q92499-1]

    Protocols and materials databases

    Antibodypedia a portal for validated antibodies

    More...
    Antibodypediai
    3224, 233 antibodies

    Genome annotation databases

    EnsembliENST00000233084; ENSP00000233084; ENSG00000079785 [Q92499-1]
    ENST00000381341; ENSP00000370745; ENSG00000079785 [Q92499-1]
    GeneIDi1653
    KEGGihsa:1653
    UCSCiuc002rce.5, human [Q92499-1]

    Organism-specific databases

    Comparative Toxicogenomics Database

    More...
    CTDi
    1653
    DisGeNETi1653
    EuPathDBiHostDB:ENSG00000079785.14

    GeneCards: human genes, protein and diseases

    More...
    GeneCardsi
    DDX1
    HGNCiHGNC:2734, DDX1
    HPAiENSG00000079785, Low tissue specificity
    MIMi601257, gene
    neXtProtiNX_Q92499
    OpenTargetsiENSG00000079785
    PharmGKBiPA27199

    GenAtlas: human gene database

    More...
    GenAtlasi
    Search...

    Phylogenomic databases

    eggNOGiKOG0349, Eukaryota
    GeneTreeiENSGT00940000155678
    HOGENOMiCLU_016321_0_0_1
    InParanoidiQ92499
    KOiK13177
    OMAiDGVHDRD
    PhylomeDBiQ92499
    TreeFamiTF106114

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000079785-MONOMER
    PathwayCommonsiQ92499
    ReactomeiR-HSA-6784531, tRNA processing in the nucleus
    SIGNORiQ92499

    Miscellaneous databases

    BioGRID ORCS database of CRISPR phenotype screens

    More...
    BioGRID-ORCSi
    1653, 316 hits in 884 CRISPR screens

    ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

    More...
    ChiTaRSi
    DDX1, human

    The Gene Wiki collection of pages on human genes and proteins

    More...
    GeneWikii
    DDX1

    Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

    More...
    GenomeRNAii
    1653
    PharosiQ92499, Tbio

    Protein Ontology

    More...
    PROi
    PR:Q92499
    RNActiQ92499, protein

    The Stanford Online Universal Resource for Clones and ESTs

    More...
    SOURCEi
    Search...

    Gene expression databases

    BgeeiENSG00000079785, Expressed in tendon and 247 other tissues
    ExpressionAtlasiQ92499, baseline and differential
    GenevisibleiQ92499, HS

    Family and domain databases

    Gene3Di2.60.120.920, 1 hit
    InterProiView protein in InterPro
    IPR001870, B30.2/SPRY
    IPR043136, B30.2/SPRY_sf
    IPR013320, ConA-like_dom_sf
    IPR011545, DEAD/DEAH_box_helicase_dom
    IPR014001, Helicase_ATP-bd
    IPR001650, Helicase_C
    IPR027417, P-loop_NTPase
    IPR014014, RNA_helicase_DEAD_Q_motif
    IPR003877, SPRY_dom
    PfamiView protein in Pfam
    PF00270, DEAD, 1 hit
    PF00271, Helicase_C, 1 hit
    PF00622, SPRY, 1 hit
    SMARTiView protein in SMART
    SM00487, DEXDc, 1 hit
    SM00490, HELICc, 1 hit
    SM00449, SPRY, 1 hit
    SUPFAMiSSF49899, SSF49899, 1 hit
    SSF52540, SSF52540, 2 hits
    PROSITEiView protein in PROSITE
    PS50188, B302_SPRY, 1 hit
    PS51192, HELICASE_ATP_BIND_1, 2 hits
    PS51194, HELICASE_CTER, 1 hit
    PS51195, Q_MOTIF, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDDX1_HUMAN
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q92499
    Secondary accession number(s): B4DME8, B4DPN6
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 7, 2020
    This is version 196 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Reference proteome

    Documents

    1. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
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