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Protein

ATP-dependent 6-phosphofructokinase subunit alpha

Gene

PFK1

Organism
Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Involved in the modulation of glucose-induced microautophagy of peroxisomes independent of its ability to metabolize glucose intermediates.UniRule annotation1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PAS_chr3_0456)
  3. ATP-dependent 6-phosphofructokinase subunit beta (PFK2), ATP-dependent 6-phosphofructokinase subunit alpha (PFK1), ATP-dependent 6-phosphofructokinase subunit gamma (PFK3)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATP; via amide nitrogenUniRule annotation1
Metal bindingi314Magnesium; catalyticUniRule annotation1
Active sitei361Proton acceptorUniRule annotation1
Binding sitei396Substrate; shared with subunit betaUniRule annotation1
Binding sitei460SubstrateUniRule annotation1
Binding sitei487Substrate; shared with subunit betaUniRule annotation1
Binding sitei670Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei765Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation1
Binding sitei832Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei858Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation1
Binding sitei963Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 284ATPUniRule annotation2
Nucleotide bindingi313 – 316ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Protein family/group databases

MoonDBiQ92448 Curated
MoonProtiQ92448

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation (EC:2.7.1.11UniRule annotation)
Alternative name(s):
ATP-dependent 6-phosphofructokinase 1UniRule annotation
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Glucose-induced selective autophagy 1 protein
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:PFK1
Synonyms:GSA1
Ordered Locus Names:PAS_chr2-1_0402
OrganismiKomagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
Proteomesi
  • UP000000314 Componenti: Chromosome 2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi361D → S: Abolishes catalytic activity, but not the ability to modulate glucose-induced microautophagy of peroxisomes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120421 – 989ATP-dependent 6-phosphofructokinase subunit alphaAdd BLAST989

Proteomic databases

PRIDEiQ92448

Interactioni

Subunit structurei

Heterododecamer of 4 alpha, 4 beta and 4 gamma chains. The gamma chain bridges the N-terminal halves of the alpha and beta subunits (By similarity).By similarity

Protein-protein interaction databases

STRINGi644223.XP_002491303.1

Structurei

3D structure databases

ProteinModelPortaliQ92448
SMRiQ92448
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 585N-terminal catalytic PFK domain 1Add BLAST585
Regioni359 – 361Substrate bindingUniRule annotation3
Regioni403 – 405Substrate bindingUniRule annotation3
Regioni493 – 496Substrate bindingUniRule annotation4
Regioni586 – 599Interdomain linkerAdd BLAST14
Regioni600 – 989C-terminal regulatory PFK domain 2Add BLAST390
Regioni727 – 731Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni772 – 774Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni864 – 867Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
HOGENOMiHOG000200154
KOiK00850
OMAiKQYDELC

Family and domain databases

HAMAPiMF_03184 Phosphofructokinase_I_E, 1 hit
InterProiView protein in InterPro
IPR009161 6-Pfructokinase_euk
IPR022953 ATP_PFK
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 2 hits
PIRSFiPIRSF000533 ATP_PFK_euk, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 2 hits
TIGRFAMsiTIGR02478 6PF1K_euk, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q92448-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPEPSISALS FTSFVTNDDK LFEETFNFYT KLGFHATRSY VKDNRSDFEL
60 70 80 90 100
TGISTDSIKE IWLESFPLSE VVETSAGREL RKPLQESVGY QSEALLGYSP
110 120 130 140 150
YQSDGVVIKL RLSNHDLQKN KDLPGEVTFF TASIDKLRAK LIEIGAEIIP
160 170 180 190 200
SEIDLVEFST KDPMGDVISF SSYPSLSSKK ITSPDFFLHP KKEVRSQESI
210 220 230 240 250
VEQVKSEEGK KKIAIITSGG DAPGMNAAVR AVTRAGIFYG CKVYACYEGY
260 270 280 290 300
TGLVKGGDML KELQWQDVRG LLSIGGTIIG TARSKEFRER WGRLQACYNM
310 320 330 340 350
VSNGIDALVV CGGDGSLTGA DLFRNEWPEL IKELLGEGKI TKEQYETHRN
360 370 380 390 400
LTIVGLVGSI DNDMCGTDST IGAYSSLERI IELVDYIDAT AASHSRAFVV
410 420 430 440 450
EVMGRHCGWL GLMSGIATGA DYIFIPERPP SETNWKDDLK KVCLRHREKG
460 470 480 490 500
RRKTTVIVAE GAIDDQLNPI TSEEVKDVLV EIGLDTRITR LGHVQRGGAP
510 520 530 540 550
CAFDRFLATV QGVDAVRAVL ESTPAIPSPV ISILENKIVR QPLVESVAQT
560 570 580 590 600
KTVSDAIEAK DFDKALKLRD QEFATSYESF LSVSKYDDGS YLVPESSRLN
610 620 630 640 650
IAIIHVGAPT SALNPATRVA TLNSLAKGHR VFAIRNGFAG LIRHGAVREL
660 670 680 690 700
NWIDVEDWHN TGGSEIGTNR SLPSDDMGTV AYYFQQYKFD GLIIIGGFEA
710 720 730 740 750
FTALYQLDAA RAQYPIFNIP MCCLPATVSN NVPGTEYSLG SDTCLNTLSG
760 770 780 790 800
YCDAVKQSAS ASRRRTFVVE VQGGYSGYLA SYAGLITGAL AVYTPENPIN
810 820 830 840 850
LQTVQEDIEL LTRTYEEDDG KNRSGKIFIH NEKASKVYTT DLIAAIIGEA
860 870 880 890 900
GKGRFESRTA VPGHVQQGKS PSSIDRVNAC RLAIKCCNFI EDANFQVKHN
910 920 930 940 950
ANLSADERHL RFFYDDGVKT SAVSGKSSVI DDNTSVVIGI QGSEVTFTPV
960 970 980
KQLWEKETHH KWRKGKNVHW EQLNIVSDLL SGRLSIRTT
Length:989
Mass (Da):108,817
Last modified:July 9, 2014 - v2
Checksum:i6708A34ED94C9DD4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178S → SK in AAB97871 (PubMed:9296392).Curated1
Sequence conflicti570D → E in AAB97871 (PubMed:9296392).Curated1
Sequence conflicti914Y → C in AAB97871 (PubMed:9296392).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73376 Genomic DNA Translation: AAB97871.1
FN392320 Genomic DNA Translation: CAY69023.1
RefSeqiXP_002491303.1, XM_002491258.1

Genome annotation databases

EnsemblFungiiCAY69023; CAY69023; PAS_chr2-1_0402
GeneIDi8198870
KEGGippa:PAS_chr2-1_0402

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73376 Genomic DNA Translation: AAB97871.1
FN392320 Genomic DNA Translation: CAY69023.1
RefSeqiXP_002491303.1, XM_002491258.1

3D structure databases

ProteinModelPortaliQ92448
SMRiQ92448
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi644223.XP_002491303.1

Protein family/group databases

MoonDBiQ92448 Curated
MoonProtiQ92448

Proteomic databases

PRIDEiQ92448

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAY69023; CAY69023; PAS_chr2-1_0402
GeneIDi8198870
KEGGippa:PAS_chr2-1_0402

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
HOGENOMiHOG000200154
KOiK00850
OMAiKQYDELC

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Family and domain databases

HAMAPiMF_03184 Phosphofructokinase_I_E, 1 hit
InterProiView protein in InterPro
IPR009161 6-Pfructokinase_euk
IPR022953 ATP_PFK
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 2 hits
PIRSFiPIRSF000533 ATP_PFK_euk, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 2 hits
TIGRFAMsiTIGR02478 6PF1K_euk, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA1_KOMPG
AccessioniPrimary (citable) accession number: Q92448
Secondary accession number(s): C4R0J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 9, 2014
Last modified: November 7, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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