UniProtKB - Q923X1 (AGRL4_MOUSE)
Protein
Adhesion G protein-coupled receptor L4
Gene
Adgrl4
Organism
Mus musculus (Mouse)
Status
Functioni
Endothelial orphan receptor that acts as a key regulator of angiogenesis.By similarity1 Publication
GO - Molecular functioni
- calcium ion binding Source: InterPro
- G protein-coupled receptor activity Source: GO_Central
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
- cell surface receptor signaling pathway Source: InterPro
- protein-containing complex assembly Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Ligand | Calcium |
Protein family/group databases
MEROPSi | P02.013 |
Names & Taxonomyi
Protein namesi | Recommended name: Adhesion G protein-coupled receptor L4Alternative name(s): EGF, latrophilin seven transmembrane domain-containing protein 1 |
Gene namesi | Name:Adgrl4 Synonyms:Eltd1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2655562, Adgrl4 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cytoplasmic vesicle Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 481 | ExtracellularCuratedAdd BLAST | 462 | |
Transmembranei | 482 – 502 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 503 – 513 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 514 – 534 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 535 – 548 | ExtracellularCuratedAdd BLAST | 14 | |
Transmembranei | 549 – 569 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 570 – 581 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 582 – 602 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 603 – 622 | ExtracellularCuratedAdd BLAST | 20 | |
Transmembranei | 623 – 643 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 644 – 667 | CytoplasmicCuratedAdd BLAST | 24 | |
Transmembranei | 668 – 688 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 689 – 695 | ExtracellularCurated | 7 | |
Transmembranei | 696 – 716 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 717 – 739 | CytoplasmicCuratedAdd BLAST | 23 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype but deficient mice present increased cardiac hypertrophy in response to pressure overload.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000012871 | 20 – 739 | Adhesion G protein-coupled receptor L4Add BLAST | 720 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 21 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 22 ↔ 33 | PROSITE-ProRule annotation | ||
Disulfide bondi | 27 ↔ 42 | PROSITE-ProRule annotation | ||
Disulfide bondi | 44 ↔ 56 | PROSITE-ProRule annotation | ||
Disulfide bondi | 62 ↔ 74 | PROSITE-ProRule annotation | ||
Disulfide bondi | 68 ↔ 83 | PROSITE-ProRule annotation | ||
Disulfide bondi | 85 ↔ 106 | PROSITE-ProRule annotation | ||
Disulfide bondi | 112 ↔ 124 | PROSITE-ProRule annotation | ||
Disulfide bondi | 118 ↔ 133 | PROSITE-ProRule annotation | ||
Disulfide bondi | 135 ↔ 156 | PROSITE-ProRule annotation | ||
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 226 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 237 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 422 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 430 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 444 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylated.By similarity
Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 455 – 456 | CleavageBy similarity | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | non-CPTAC-3686 |
MaxQBi | Q923X1 |
PaxDbi | Q923X1 |
PRIDEi | Q923X1 |
ProteomicsDBi | 296141 |
PTM databases
GlyGeni | Q923X1, 8 sites |
PhosphoSitePlusi | Q923X1 |
Expressioni
Inductioni
Induced by VEGF and FGF2.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000039167, Expressed in brain blood vessel and 240 other tissues |
Genevisiblei | Q923X1, MM |
Interactioni
Subunit structurei
Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked.
By similarityProtein-protein interaction databases
BioGRIDi | 228419, 6 interactors |
STRINGi | 10090.ENSMUSP00000041939 |
Miscellaneous databases
RNActi | Q923X1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 57 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 58 – 107 | EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 50 | |
Domaini | 108 – 157 | EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 50 | |
Domaini | 415 – 467 | GPSPROSITE-ProRule annotationAdd BLAST | 53 |
Domaini
The transmembrane domain is not required for cleavage, but it is required for dimer formation.By similarity
Sequence similaritiesi
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.Curated
Keywords - Domaini
EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4193, Eukaryota KOG4291, Eukaryota |
GeneTreei | ENSGT00940000158252 |
HOGENOMi | CLU_002753_3_8_1 |
InParanoidi | Q923X1 |
OMAi | SQCAFWN |
OrthoDBi | 388923at2759 |
TreeFami | TF316380 |
Family and domain databases
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR032471, GAIN_dom_N IPR017981, GPCR_2-like IPR000832, GPCR_2_secretin-like IPR017983, GPCR_2_secretin-like_CS IPR000203, GPS |
Pfami | View protein in Pfam PF00002, 7tm_2, 1 hit PF07645, EGF_CA, 2 hits PF16489, GAIN, 1 hit PF01825, GPS, 1 hit |
PRINTSi | PR00249, GPCRSECRETIN |
SMARTi | View protein in SMART SM00181, EGF, 3 hits SM00179, EGF_CA, 2 hits SM00303, GPS, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 2 hits PS01186, EGF_2, 1 hit PS50026, EGF_3, 3 hits PS01187, EGF_CA, 2 hits PS00650, G_PROTEIN_RECEP_F2_2, 1 hit PS50261, G_PROTEIN_RECEP_F2_4, 1 hit PS50221, GPS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q923X1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLLPLLVGF STLLNCSYTQ NCSKTTCLPN AKCEVHNGVE ACFCSQGYSG
60 70 80 90 100
NGVTICEDID ECSESSVCGD HAVCENVNGG FSCFCREGYQ TATGKSQFTP
110 120 130 140 150
NDGSYCQDID ECSESSVCGD HAVCENVNGG FSCFCREGYQ TATGKSQFTP
160 170 180 190 200
NDGSYCQESM NSNCHLEHAC IAANINKTLK RIGPITEQTT LLQEIYRNSE
210 220 230 240 250
AELSLMDIVT YIEILTESSS LLGHPNSTTS YKDAHFNSTL TEFGETINNF
260 270 280 290 300
VERSTHKMWD QLPTNHRRLH LTKLMHTAEL VTLQIAQNTQ KNSQFDMNST
310 320 330 340 350
DLALKVFAFD STHMKHAHPH MNVDGGYVKI SPRRKAAHGT TGNVVVAFLC
360 370 380 390 400
YKSIGPLLSS SDNFLLDTQN DNSEGKEKVI SSVISASISS NPPTLYELEK
410 420 430 440 450
ITFTLSHVKL SDKHRTQCAF WNYSVDAMNN GSWSTEGCEL THSNDTHTSC
460 470 480 490 500
RCSHLTHFAI LMSSTSSIGI KDYNILTRIT QLGIIISLIC LAICIFTFWF
510 520 530 540 550
FSEIQSTRTT IHKNLCCSLF LAELVFLIGI NINTNKLVCS IIAGLLHYFF
560 570 580 590 600
LAAFAWMCIE GIHLYLIVVG VIYNKGFLHK NFYIFGYLSP AVVVGFSASL
610 620 630 640 650
GYRYYGTTKV CWLSTENNFI WSFIGPACLI ILVNLLAFGV IIYKVFRHTA
660 670 680 690 700
GLKPEVSCYE NIRSCARGAL ALLFLLGTTW IFGVLHVVHA SVVTAYLFTV
710 720 730
SNAFQGMFIF LFLCVLSRKI QEEYYRLFKN VPCCFGCLR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JGY1 | A0A0G2JGY1_MOUSE | Adhesion G protein-coupled receptor... | Adgrl4 | 181 | Annotation score: | ||
A0A571BEP4 | A0A571BEP4_MOUSE | Adhesion G protein-coupled receptor... | Adgrl4 | 20 | Annotation score: | ||
A0A571BF07 | A0A571BF07_MOUSE | Adhesion G protein-coupled receptor... | Adgrl4 | 91 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 289 | T → I in AAK62363 (PubMed:11438738).Curated | 1 | |
Sequence conflicti | 313 | Missing in AAH17134 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF385682 mRNA Translation: AAK62363.1 AC127233 Genomic DNA No translation available. BC017134 mRNA Translation: AAH17134.1 |
CCDSi | CCDS17910.1 |
RefSeqi | NP_573485.2, NM_133222.3 |
Genome annotation databases
Ensembli | ENSMUST00000046977; ENSMUSP00000041939; ENSMUSG00000039167 |
GeneIDi | 170757 |
KEGGi | mmu:170757 |
UCSCi | uc008rsj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF385682 mRNA Translation: AAK62363.1 AC127233 Genomic DNA No translation available. BC017134 mRNA Translation: AAH17134.1 |
CCDSi | CCDS17910.1 |
RefSeqi | NP_573485.2, NM_133222.3 |
3D structure databases
SMRi | Q923X1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228419, 6 interactors |
STRINGi | 10090.ENSMUSP00000041939 |
Protein family/group databases
MEROPSi | P02.013 |
GPCRDBi | Search... |
PTM databases
GlyGeni | Q923X1, 8 sites |
PhosphoSitePlusi | Q923X1 |
Proteomic databases
CPTACi | non-CPTAC-3686 |
MaxQBi | Q923X1 |
PaxDbi | Q923X1 |
PRIDEi | Q923X1 |
ProteomicsDBi | 296141 |
Protocols and materials databases
ABCDi | Q923X1, 6 sequenced antibodies |
Antibodypediai | 9366, 217 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000046977; ENSMUSP00000041939; ENSMUSG00000039167 |
GeneIDi | 170757 |
KEGGi | mmu:170757 |
UCSCi | uc008rsj.2, mouse |
Organism-specific databases
CTDi | 64123 |
MGIi | MGI:2655562, Adgrl4 |
Phylogenomic databases
eggNOGi | KOG4193, Eukaryota KOG4291, Eukaryota |
GeneTreei | ENSGT00940000158252 |
HOGENOMi | CLU_002753_3_8_1 |
InParanoidi | Q923X1 |
OMAi | SQCAFWN |
OrthoDBi | 388923at2759 |
TreeFami | TF316380 |
Miscellaneous databases
BioGRID-ORCSi | 170757, 0 hits in 52 CRISPR screens |
ChiTaRSi | Adgrl4, mouse |
PROi | PR:Q923X1 |
RNActi | Q923X1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039167, Expressed in brain blood vessel and 240 other tissues |
Genevisiblei | Q923X1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR032471, GAIN_dom_N IPR017981, GPCR_2-like IPR000832, GPCR_2_secretin-like IPR017983, GPCR_2_secretin-like_CS IPR000203, GPS |
Pfami | View protein in Pfam PF00002, 7tm_2, 1 hit PF07645, EGF_CA, 2 hits PF16489, GAIN, 1 hit PF01825, GPS, 1 hit |
PRINTSi | PR00249, GPCRSECRETIN |
SMARTi | View protein in SMART SM00181, EGF, 3 hits SM00179, EGF_CA, 2 hits SM00303, GPS, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 2 hits PS01186, EGF_2, 1 hit PS50026, EGF_3, 3 hits PS01187, EGF_CA, 2 hits PS00650, G_PROTEIN_RECEP_F2_2, 1 hit PS50261, G_PROTEIN_RECEP_F2_4, 1 hit PS50221, GPS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AGRL4_MOUSE | |
Accessioni | Q923X1Primary (citable) accession number: Q923X1 Secondary accession number(s): E9QLT4, Q91W44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 152 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families