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Protein

Disintegrin and metalloproteinase domain-containing protein 33

Gene

Adam33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Zinc; in inhibited formBy similarity1
Metal bindingi346Zinc; catalyticBy similarity1
Active sitei347PROSITE-ProRule annotation1
Metal bindingi350Zinc; catalyticBy similarity1
Metal bindingi356Zinc; catalyticBy similarity1

GO - Molecular functioni

  • metalloendopeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM12.244

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 33 (EC:3.4.24.-)
Short name:
ADAM 33
Gene namesi
Name:Adam33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1341813 Adam33

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 702ExtracellularSequence analysisAdd BLAST673
Transmembranei703 – 723HelicalSequence analysisAdd BLAST21
Topological domaini724 – 797CytoplasmicSequence analysisAdd BLAST74

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002914430 – 204By similarityAdd BLAST175
ChainiPRO_0000029145205 – 797Disintegrin and metalloproteinase domain-containing protein 33Add BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi232N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi277N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi321 ↔ 405By similarity
Disulfide bondi361 ↔ 389By similarity
Disulfide bondi362 ↔ 372By similarity
Glycosylationi408N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi449N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi476 ↔ 496By similarity
Glycosylationi645N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi654 ↔ 664By similarity
Disulfide bondi658 ↔ 670By similarity
Disulfide bondi672 ↔ 681By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ923W9
PRIDEiQ923W9

PTM databases

PhosphoSitePlusiQ923W9

Expressioni

Gene expression databases

BgeeiENSMUSG00000027318 Expressed in 106 organ(s), highest expression level in ascending aorta
CleanExiMM_ADAM33
ExpressionAtlasiQ923W9 baseline and differential
GenevisibleiQ923W9 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105861

Structurei

3D structure databases

ProteinModelPortaliQ923W9
SMRiQ923W9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 410Peptidase M12BPROSITE-ProRule annotationAdd BLAST200
Domaini418 – 504DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini650 – 682EGF-likePROSITE-ProRule annotationAdd BLAST33

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi132 – 139Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi504 – 649Cys-richAdd BLAST146
Compositional biasi767 – 772Poly-Pro6

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607 Eukaryota
ENOG410XX2M LUCA
GeneTreeiENSGT00910000144014
HOGENOMiHOG000230883
HOVERGENiHBG006978
InParanoidiQ923W9
KOiK08616
OMAiPDGCCVE
OrthoDBiEOG091G01NX
PhylomeDBiQ923W9
TreeFamiTF314733

Family and domain databases

CDDicd04269 ZnMc_adamalysin_II_like, 1 hit
Gene3Di3.40.390.10, 1 hit
4.10.70.10, 1 hit
InterProiView protein in InterPro
IPR006586 ADAM_Cys-rich
IPR018358 Disintegrin_CS
IPR001762 Disintegrin_dom
IPR036436 Disintegrin_dom_sf
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR034027 Reprolysin_adamalysin
PfamiView protein in Pfam
PF08516 ADAM_CR, 1 hit
PF00200 Disintegrin, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
PRINTSiPR00289 DISINTEGRIN
SMARTiView protein in SMART
SM00608 ACR, 1 hit
SM00050 DISIN, 1 hit
SUPFAMiSSF57552 SSF57552, 1 hit
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS00427 DISINTEGRIN_1, 1 hit
PS50214 DISINTEGRIN_2, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q923W9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSRCGRPGG SPVLLLLPLL LPSCPLRSAR MFPGNAHGEL VTPHWILEGR
60 70 80 90 100
LWLKVTLEEP ILKPDSVLVA LEAEGQDLLL ELEKKHKLLA PGYTETHYRP
110 120 130 140 150
DGHPVVLSPN HTDHCQYHGR VRGFRESWVV LSTCSGMSGL IVLSSKVSYY
160 170 180 190 200
LQPRTPGDTK DFPTHEIFRM EQLFTWRGVQ RDKNSQYKAG MASLPHVPQS
210 220 230 240 250
RVRREARRSP RYLELYIVAD HTLFLLQHQN LNHTRQRLLE VANCVDQILR
260 270 280 290 300
TLDIQLVLTG LEVWTEQDLS RITQDANETL WAFLQWRRGV WARRPHDSTQ
310 320 330 340 350
LLTGRTFQGT TVGLAPVEGI CRAESSGGVS TDHSELPIGT AATMAHEIGH
360 370 380 390 400
SLGLHHDPEG CCVQADAEQG GCVMEAATGH PFPRVFSACS RRQLRTFFRK
410 420 430 440 450
GGGPCLSNTS APGLLVLPSR CGNGFLEAGE ECDCGSGQKC PDPCCFAHNC
460 470 480 490 500
SLRAGAQCAH GDCCARCLLK SAGTPCRPAA TDCDLPEFCT GTSPYCPADV
510 520 530 540 550
YLLDGSPCAE GRGYCLDGWC PTLEQQCQQL WGPGSKPAPE PCFQQMNSMG
560 570 580 590 600
NSQGNCGQDH KGSFLPCAQR DALCGKLLCQ GGEPNPLVPH IVTMDSTILL
610 620 630 640 650
EGREVVCRGA FVLPDSHLDQ LDLGLVEPGT GCGPRMVCQD RHCQNATSQE
660 670 680 690 700
LERCLTACHN GGVCNSNRNC HCAAGWAPPF CDKPGLGGSV DSGPAQSANR
710 720 730 740 750
DAFPLAMLLS FLLPLLPGAG LAWCYYQLPT FCHRRGLCCR RDPLWNRDIP
760 770 780 790
LGSVHPVEFG SIITGEPSPP PPWTSCQQRS HPPSLDLLSD PANSELT
Length:797
Mass (Da):86,971
Last modified:July 27, 2011 - v3
Checksum:iB332EA909514E626
GO
Isoform 2 (identifier: Q923W9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     637-662: Missing.

Show »
Length:771
Mass (Da):84,115
Checksum:iDAFFBA1D4416B5D2
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7CIM2F7CIM2_MOUSE
Disintegrin and metalloproteinase d...
Adam33
533Annotation score:
F6Z4C4F6Z4C4_MOUSE
Disintegrin and metalloproteinase d...
Adam33
169Annotation score:
V9GXX4V9GXX4_MOUSE
Disintegrin and metalloproteinase d...
Adam33
60Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68L → P in BAB84336 (PubMed:11814695).Curated1
Sequence conflicti68L → P in BAB84337 (PubMed:11814695).Curated1
Sequence conflicti319 – 326GICRAESS → DMPRGELSF in AAK67164 (Ref. 5) Curated8
Sequence conflicti725Missing in AAK67164 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005496637 – 662Missing in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059633 mRNA Translation: BAB84337.1
AB059632 mRNA Translation: BAB84336.1
AF472524 mRNA Translation: AAL79834.1
AL833771 Genomic DNA Translation: CAM18045.1
CH466519 Genomic DNA Translation: EDL28296.1
AF386072 mRNA Translation: AAK67164.1
CCDSiCCDS16753.1 [Q923W9-1]
CCDS50716.1 [Q923W9-2]
RefSeqiNP_291093.2, NM_033615.3 [Q923W9-1]
UniGeneiMm.108550

Genome annotation databases

EnsembliENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318 [Q923W9-2]
ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318 [Q923W9-1]
ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318 [Q923W9-1]
GeneIDi110751
KEGGimmu:110751
UCSCiuc008mkm.2 mouse [Q923W9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059633 mRNA Translation: BAB84337.1
AB059632 mRNA Translation: BAB84336.1
AF472524 mRNA Translation: AAL79834.1
AL833771 Genomic DNA Translation: CAM18045.1
CH466519 Genomic DNA Translation: EDL28296.1
AF386072 mRNA Translation: AAK67164.1
CCDSiCCDS16753.1 [Q923W9-1]
CCDS50716.1 [Q923W9-2]
RefSeqiNP_291093.2, NM_033615.3 [Q923W9-1]
UniGeneiMm.108550

3D structure databases

ProteinModelPortaliQ923W9
SMRiQ923W9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105861

Protein family/group databases

MEROPSiM12.244

PTM databases

PhosphoSitePlusiQ923W9

Proteomic databases

PaxDbiQ923W9
PRIDEiQ923W9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318 [Q923W9-2]
ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318 [Q923W9-1]
ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318 [Q923W9-1]
GeneIDi110751
KEGGimmu:110751
UCSCiuc008mkm.2 mouse [Q923W9-1]

Organism-specific databases

CTDi80332
MGIiMGI:1341813 Adam33

Phylogenomic databases

eggNOGiKOG3607 Eukaryota
ENOG410XX2M LUCA
GeneTreeiENSGT00910000144014
HOGENOMiHOG000230883
HOVERGENiHBG006978
InParanoidiQ923W9
KOiK08616
OMAiPDGCCVE
OrthoDBiEOG091G01NX
PhylomeDBiQ923W9
TreeFamiTF314733

Miscellaneous databases

PROiPR:Q923W9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027318 Expressed in 106 organ(s), highest expression level in ascending aorta
CleanExiMM_ADAM33
ExpressionAtlasiQ923W9 baseline and differential
GenevisibleiQ923W9 MM

Family and domain databases

CDDicd04269 ZnMc_adamalysin_II_like, 1 hit
Gene3Di3.40.390.10, 1 hit
4.10.70.10, 1 hit
InterProiView protein in InterPro
IPR006586 ADAM_Cys-rich
IPR018358 Disintegrin_CS
IPR001762 Disintegrin_dom
IPR036436 Disintegrin_dom_sf
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR034027 Reprolysin_adamalysin
PfamiView protein in Pfam
PF08516 ADAM_CR, 1 hit
PF00200 Disintegrin, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
PRINTSiPR00289 DISINTEGRIN
SMARTiView protein in SMART
SM00608 ACR, 1 hit
SM00050 DISIN, 1 hit
SUPFAMiSSF57552 SSF57552, 1 hit
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS00427 DISINTEGRIN_1, 1 hit
PS50214 DISINTEGRIN_2, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiADA33_MOUSE
AccessioniPrimary (citable) accession number: Q923W9
Secondary accession number(s): Q8R5G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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