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Protein

Trimethylguanosine synthase

Gene

Tgs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation (By similarity).By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,7)G(5')pppR-RNA.
S-adenosyl-L-methionine + m(2,7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,2,7)G(5')pppR-RNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei711S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-191859 snRNP Assembly
R-MMU-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylguanosine synthase (EC:2.1.1.-)
Alternative name(s):
Nuclear receptor coactivator 6-interacting protein
PRIP-interacting protein with methyltransferase motif
Short name:
PIMT
Short name:
PIPMT
Gene namesi
Name:Tgs1
Synonyms:Ncoa6ip, Pimt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2151797 Tgs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002044691 – 853Trimethylguanosine synthaseAdd BLAST853

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphothreonineBy similarity1
Modified residuei81PhosphoserineBy similarity1
Modified residuei85PhosphoserineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Modified residuei139PhosphoserineBy similarity1
Modified residuei144PhosphotyrosineBy similarity1
Modified residuei152PhosphoserineCombined sources1
Modified residuei405PhosphoserineBy similarity1
Modified residuei431PhosphoserineCombined sources1
Modified residuei572PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ923W1
MaxQBiQ923W1
PaxDbiQ923W1
PeptideAtlasiQ923W1
PRIDEiQ923W1

PTM databases

iPTMnetiQ923W1
PhosphoSitePlusiQ923W1

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000028233 Expressed in 283 organ(s), highest expression level in skin of back
GenevisibleiQ923W1 MM

Interactioni

Subunit structurei

May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN (By similarity).By similarity

Protein-protein interaction databases

BioGridi228104, 20 interactors
IntActiQ923W1, 21 interactors
STRINGi10090.ENSMUSP00000054112

Structurei

3D structure databases

ProteinModelPortaliQ923W1
SMRiQ923W1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi612 – 622Lys-richAdd BLAST11

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2730 Eukaryota
ENOG410XRUD LUCA
GeneTreeiENSGT00390000018056
HOGENOMiHOG000154561
HOVERGENiHBG059797
InParanoidiQ923W1
KOiK14292
OMAiMNTRNKV
OrthoDBiEOG091G0DYO
PhylomeDBiQ923W1
TreeFamiTF313065

Family and domain databases

InterProiView protein in InterPro
IPR019012 RNA_cap_Gua-N2-MeTrfase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF09445 Methyltransf_15, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q923W1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCCEKWNHVA EMLLFIEDRE EEYKILCLCS RAFVEDRKLY NLGLKGYYVK
60 70 80 90 100
SSGNNAGDQG TEEEEDGHSN GTAESHSPNE SDLDSEAKLM RSMGLPIQFG
110 120 130 140 150
RMSSHENFEM SMNARNKAKV KQKRRKHQKR YLDEMVRESW RNDYEEDDLV
160 170 180 190 200
VSDDPSSVEH CENNRTCEIQ SKAGSEVENL PVENTLAPKL EVPENWEKYW
210 220 230 240 250
NEYGEGLLWQ SWQEKYPDQT LSSEPWNLPD TKEEWEQHYS QLYWYYLEQF
260 270 280 290 300
QYWEAQGWTF TASQNCDKDV YTSHTEVDQN AESSLKADVM TFSSSPNIVE
310 320 330 340 350
DEIPGSNDND HNEIITAINN ITVSAEKVEQ SQLDSSQHCD EPLSEITGKE
360 370 380 390 400
CPASGGSDSC NGTPKENDIS ENRSSDQPAK ELQESSGTNK GKHRPHHNGA
410 420 430 440 450
DGHESDDDPP EHKPSKVKRS HELDVDENPD SEVDDNGFLL GFKHGSGQKY
460 470 480 490 500
GGIPNFSHRQ VRYLEKNVKY KSKYLDLRKQ MPVKSKHILF TEDSGKPFVV
510 520 530 540 550
CKSKVRSKVE KFLKWVNERV DEETSQDSLS QNKMQDTCTS SDSEEQDMSL
560 570 580 590 600
EKADNLMETR DPEPEKCQII SSATELEAEK SEVGSLVATV PENCSTEEIP
610 620 630 640 650
NSPHAETEVE IKKKKKKNKN KKINDLPPEI ASVPELAKYW AQRYRLFSRF
660 670 680 690 700
DDGIKLDKEG WFSVTPEKIA EHIAGRVSQA FRCDVVVDAF CGVGGNTIQF
710 720 730 740 750
ALTGKRVIAI DIDPVKIDLA RNNAEVYGIA DKIEFICGDF LLLAPCLKAD
760 770 780 790 800
VVFLSPPWGG PDYATAETFD IRTMMSPDGF EIFRLSQKIT NNIVYFLPRN
810 820 830 840 850
ADIDQVASLA GLGGQVEIEQ NFLNNKLKTI TAYFGDLIRR PALLKTSTSE

AEV
Length:853
Mass (Da):96,790
Last modified:April 3, 2007 - v2
Checksum:iA69974BE660DA3AF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti512F → L in AAH75728 (PubMed:15489334).Curated1
Sequence conflicti633V → G in AAK84355 (PubMed:11517327).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF389908 mRNA Translation: AAK84355.1
AL732617 Genomic DNA No translation available.
BC026368 mRNA Translation: AAH26368.1
BC058183 mRNA Translation: AAH58183.1
BC075728 mRNA Translation: AAH75728.1
CCDSiCCDS17938.1
RefSeqiNP_473430.3, NM_054089.4
UniGeneiMm.171323

Genome annotation databases

EnsembliENSMUST00000052712; ENSMUSP00000054112; ENSMUSG00000028233
GeneIDi116940
KEGGimmu:116940
UCSCiuc008rwi.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF389908 mRNA Translation: AAK84355.1
AL732617 Genomic DNA No translation available.
BC026368 mRNA Translation: AAH26368.1
BC058183 mRNA Translation: AAH58183.1
BC075728 mRNA Translation: AAH75728.1
CCDSiCCDS17938.1
RefSeqiNP_473430.3, NM_054089.4
UniGeneiMm.171323

3D structure databases

ProteinModelPortaliQ923W1
SMRiQ923W1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228104, 20 interactors
IntActiQ923W1, 21 interactors
STRINGi10090.ENSMUSP00000054112

PTM databases

iPTMnetiQ923W1
PhosphoSitePlusiQ923W1

Proteomic databases

EPDiQ923W1
MaxQBiQ923W1
PaxDbiQ923W1
PeptideAtlasiQ923W1
PRIDEiQ923W1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052712; ENSMUSP00000054112; ENSMUSG00000028233
GeneIDi116940
KEGGimmu:116940
UCSCiuc008rwi.2 mouse

Organism-specific databases

CTDi96764
MGIiMGI:2151797 Tgs1

Phylogenomic databases

eggNOGiKOG2730 Eukaryota
ENOG410XRUD LUCA
GeneTreeiENSGT00390000018056
HOGENOMiHOG000154561
HOVERGENiHBG059797
InParanoidiQ923W1
KOiK14292
OMAiMNTRNKV
OrthoDBiEOG091G0DYO
PhylomeDBiQ923W1
TreeFamiTF313065

Enzyme and pathway databases

ReactomeiR-MMU-191859 snRNP Assembly
R-MMU-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Miscellaneous databases

ChiTaRSiTgs1 mouse
PROiPR:Q923W1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028233 Expressed in 283 organ(s), highest expression level in skin of back
GenevisibleiQ923W1 MM

Family and domain databases

InterProiView protein in InterPro
IPR019012 RNA_cap_Gua-N2-MeTrfase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF09445 Methyltransf_15, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTGS1_MOUSE
AccessioniPrimary (citable) accession number: Q923W1
Secondary accession number(s): A2AJF7
, Q6DI60, Q6PEA7, Q8R0W9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: April 3, 2007
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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