UniProtKB - Q923U9 (S40A1_RAT)
Solute carrier family 40 member 1
Slc40a1
Functioni
Major iron transporter that plays a key role in balancing cellular and systemic iron levels (By similarity).
Transports iron from intestinal, splenic, and hepatic cells into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages, and release of iron stores in hepatocytes (By similarity).
When iron is in excess, hepcidin/HAMP levels increase resulting in a degradation of ferroportin/SLC40A1 limiting the iron efflux to plasma (By similarity).
By similarityCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 39 | Iron 1By similarity | 1 | |
Metal bindingi | 43 | Iron 1By similarity | 1 | |
Metal bindingi | 326 | Iron 2By similarity | 1 | |
Metal bindingi | 506 | Iron 2By similarity | 1 |
GO - Molecular functioni
- ferrous iron transmembrane transporter activity Source: RGD
- identical protein binding Source: RGD
- iron ion transmembrane transporter activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- peptide hormone binding Source: RGD
GO - Biological processi
- cellular iron ion homeostasis Source: RGD
- endothelium development Source: RGD
- iron ion export across plasma membrane Source: RGD
- iron ion homeostasis Source: RGD
- iron ion transmembrane transport Source: RGD
- iron ion transport Source: RGD
- lymphocyte homeostasis Source: RGD
- multicellular organismal iron ion homeostasis Source: RGD
- negative regulation of apoptotic process Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of transcription from RNA polymerase II promoter in response to iron Source: RGD
- spleen development Source: RGD
- spleen trabecula formation Source: RGD
Keywordsi
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-425410, Metal ion SLC transporters R-RNO-917937, Iron uptake and transport |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 40 member 1Alternative name(s): CAR1 Cell adhesion regulator Ferroportin-1 |
Gene namesi | Name:Slc40a1 Synonyms:Fpn1, Fpt1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620180, Slc40a1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Localized to the basolateral membrane of polarized epithelial cells.By similarity
Plasma Membrane
- basolateral plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- synaptic vesicle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 23 | CytoplasmicBy similarityAdd BLAST | 23 | |
Transmembranei | 24 – 53 | HelicalBy similarityAdd BLAST | 30 | |
Topological domaini | 54 – 57 | ExtracellularBy similarity | 4 | |
Transmembranei | 58 – 84 | HelicalBy similarityAdd BLAST | 27 | |
Topological domaini | 85 – 87 | CytoplasmicBy similarity | 3 | |
Transmembranei | 88 – 118 | HelicalBy similarityAdd BLAST | 31 | |
Topological domaini | 119 – 126 | ExtracellularBy similarity | 8 | |
Transmembranei | 127 – 162 | HelicalBy similarityAdd BLAST | 36 | |
Topological domaini | 163 – 164 | CytoplasmicBy similarity | 2 | |
Transmembranei | 165 – 195 | HelicalBy similarityAdd BLAST | 31 | |
Topological domaini | 196 – 202 | ExtracellularBy similarity | 7 | |
Transmembranei | 203 – 229 | HelicalBy similarityAdd BLAST | 27 | |
Topological domaini | 230 – 306 | CytoplasmicBy similarityAdd BLAST | 77 | |
Transmembranei | 307 – 333 | HelicalBy similarityAdd BLAST | 27 | |
Topological domaini | 334 – 338 | ExtracellularBy similarity | 5 | |
Transmembranei | 339 – 366 | HelicalBy similarityAdd BLAST | 28 | |
Topological domaini | 367 – 368 | CytoplasmicBy similarity | 2 | |
Transmembranei | 369 – 391 | HelicalBy similarityAdd BLAST | 23 | |
Topological domaini | 392 – 452 | ExtracellularBy similarityAdd BLAST | 61 | |
Transmembranei | 453 – 482 | HelicalBy similarityAdd BLAST | 30 | |
Topological domaini | 483 – 487 | CytoplasmicBy similarity | 5 | |
Transmembranei | 488 – 512 | HelicalBy similarityAdd BLAST | 25 | |
Topological domaini | 513 – 515 | ExtracellularBy similarity | 3 | |
Transmembranei | 516 – 541 | HelicalBy similarityAdd BLAST | 26 | |
Topological domaini | 542 – 570 | CytoplasmicBy similarityAdd BLAST | 29 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191312 | 1 – 570 | Solute carrier family 40 member 1Add BLAST | 570 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q923U9 |
Interactioni
Subunit structurei
Identified in a complex with STOM.
Interacts with HAMP; this interaction promotes SLC40A1 rapid ubiquitination.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 250996, 5 interactors |
STRINGi | 10116.ENSRNOP00000005228 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2601, Eukaryota |
InParanoidi | Q923U9 |
OrthoDBi | 334057at2759 |
PhylomeDBi | Q923U9 |
Family and domain databases
CDDi | cd17480, MFS_SLC40A1_like, 1 hit |
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR009716, Ferroportin-1 IPR036259, MFS_trans_sf |
PANTHERi | PTHR11660, PTHR11660, 1 hit |
Pfami | View protein in Pfam PF06963, FPN1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTKSRDQTHQ EGCCGSLANY LTSAKFLLYL GHSLSTWGDR MWHFAVSVFL
60 70 80 90 100
VELYGNGLLL TAVYGLVVAG SVLVLGAIIG DWVDKNARLK VAQTSLVVQN
110 120 130 140 150
VSVILCGIIL MMVFLHKNEL LNMYHGWVLT VCYILIITIA NIANLASTAT
160 170 180 190 200
AITIQRDWIV VVAGENRSRL ADMNATIRRI DQLTNILAPM AVGQIMTFGS
210 220 230 240 250
PVIGCGFISG WNLVSMCVEY FLLWKVYQKT PALAVKAALK VEESELKQLT
260 270 280 290 300
SPKDTEPKPL EGTHLMGEKD SNIRELECEQ EPTCASQIAE PFRTFRDGWV
310 320 330 340 350
SYYNQPVFLA GMGLAFLYMT VLGFDCITTG YAYTQGLSGS ILSVLMGASA
360 370 380 390 400
ITGIMGTVAF TWLRRKCGLV RTGLFSGLAQ LSCLILCVIS VFMPGSPLDL
410 420 430 440 450
SVSPFEDIRS RFIHEEAVSS TTKIPETEML MSNVSNVVNT VHEMSTKSVP
460 470 480 490 500
IISVSLLFAG VIAARIGLWS FDLTVTQLLQ ENVIESERGI INGVQNSMNY
510 520 530 540 550
LLDLLHFIMV ILAPNPEAFG LLVLISVSFV AMGHLMYFRF AQKTLGNQIF
560 570
VCAPDEKEVT DESQPNTSVV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V6H7 | G3V6H7_RAT | Solute carrier family 40 protein | Slc40a1 NEWGENE_620180, rCG_22225 | 570 | Annotation score: | ||
M0R6B7 | M0R6B7_RAT | Solute carrier family 40 protein | Slc40a1 LOC100911874 | 480 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 57 | G → S in AAD00260 (Ref. 2) Curated | 1 | |
Sequence conflicti | 328 – 330 | TTG → ATV (Ref. 2) Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF394785 mRNA Translation: AAK77858.2 U76714 mRNA Translation: AAD00260.1 Frameshift. |
RefSeqi | NP_579849.2, NM_133315.2 |
Genome annotation databases
GeneIDi | 170840 |
KEGGi | rno:170840 |
UCSCi | RGD:620180, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF394785 mRNA Translation: AAK77858.2 U76714 mRNA Translation: AAD00260.1 Frameshift. |
RefSeqi | NP_579849.2, NM_133315.2 |
3D structure databases
SMRi | Q923U9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250996, 5 interactors |
STRINGi | 10116.ENSRNOP00000005228 |
Proteomic databases
PaxDbi | Q923U9 |
Genome annotation databases
GeneIDi | 170840 |
KEGGi | rno:170840 |
UCSCi | RGD:620180, rat |
Organism-specific databases
CTDi | 30061 |
RGDi | 620180, Slc40a1 |
Phylogenomic databases
eggNOGi | KOG2601, Eukaryota |
InParanoidi | Q923U9 |
OrthoDBi | 334057at2759 |
PhylomeDBi | Q923U9 |
Enzyme and pathway databases
Reactomei | R-RNO-425410, Metal ion SLC transporters R-RNO-917937, Iron uptake and transport |
Miscellaneous databases
PROi | PR:Q923U9 |
Family and domain databases
CDDi | cd17480, MFS_SLC40A1_like, 1 hit |
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR009716, Ferroportin-1 IPR036259, MFS_trans_sf |
PANTHERi | PTHR11660, PTHR11660, 1 hit |
Pfami | View protein in Pfam PF06963, FPN1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | S40A1_RAT | |
Accessioni | Q923U9Primary (citable) accession number: Q923U9 Secondary accession number(s): Q9Z1C9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families