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Protein

E3 ubiquitin-protein ligase NEURL1

Gene

Neurl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor; inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri520 – 560RING-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processNotch signaling pathway, Translation regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase NEURL1 (EC:2.3.2.27)
Alternative name(s):
Neuralized-like protein 1A
Short name:
m-neu1
Short name:
m-neuralized 1
Neuralized1
RING-type E3 ubiquitin transferase NEURL1Curated
Gene namesi
Name:Neurl1
Synonyms:Neurl, Neurl1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1334263 Neurl1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

According to PubMed:11585928, knockout male mice lacking Neurl1 are sterile due to a defect in axoneme organization in the spermatozoa that leads to compromised tail movement and sperm motility; knockout female mice lacking Neurl1 are defective in the final stages of mammary gland maturation during pregnancy. According to PubMed:11481456 knockout mice lacking Neurl1 are viable and morphologically normal but display an olfactory discrimination and are more sensitive to the effect of ethanol on motor coordination.2 Publications

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001812562 – 574E3 ubiquitin-protein ligase NEURL1Add BLAST573

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Post-translational modificationi

Myristoylation is a determinant of membrane targeting.1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ923S6
PRIDEiQ923S6

PTM databases

iPTMnetiQ923S6
PhosphoSitePlusiQ923S6

Expressioni

Tissue specificityi

Expressed in CA1 pyramidal neurons (at protein level). Expressed throughout the adult forebrain, including the cerebral cortex, amygdala, striatum, and CA1 area of the hippocampus. Expressed in sensory neurons of the olfactory epithelium, the vomeronasal organ, mammary gland and skeletal muscle.3 Publications

Developmental stagei

Expressed in the somites at 8.5 dpc onwards.2 Publications

Inductioni

Up-regulated by synaptic activity.1 Publication

Gene expression databases

BgeeiENSMUSG00000006435 Expressed in 178 organ(s), highest expression level in gastrocnemius
CleanExiMM_NEURL
GenevisibleiQ923S6 MM

Interactioni

Subunit structurei

Interacts with CPEB3 (via N-terminal domain); the interaction increases CPEB3 ubiquitination. Interacts with DLL1 (PubMed:19723503).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cpeb3Q7TN999EBI-5376802,EBI-5376779

Protein-protein interaction databases

BioGridi201732, 4 interactors
IntActiQ923S6, 7 interactors
STRINGi10090.ENSMUSP00000107439

Structurei

3D structure databases

ProteinModelPortaliQ923S6
SMRiQ923S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 217NHR 1PROSITE-ProRule annotationAdd BLAST157
Domaini292 – 447NHR 2PROSITE-ProRule annotationAdd BLAST156

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri520 – 560RING-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4172 Eukaryota
KOG4625 Eukaryota
ENOG410ZMNG LUCA
GeneTreeiENSGT00530000063117
HOGENOMiHOG000233742
InParanoidiQ923S6
KOiK01931
OMAiCPPVLPG
OrthoDBiEOG091G03VN
PhylomeDBiQ923S6
TreeFamiTF314368

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR037962 Neuralized
IPR006573 NHR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12429 PTHR12429, 1 hit
PfamiView protein in Pfam
PF07177 Neuralized, 2 hits
SMARTiView protein in SMART
SM00588 NEUZ, 2 hits
PROSITEiView protein in PROSITE
PS51065 NHR, 2 hits
PS50089 ZF_RING_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q923S6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGNNFSSVSS LQRGNPSRAS RGHPQNLKDS IGGSFPVPSH RCHHKQKHCP
60 70 80 90 100
PTLSGGGLPA TPLLFHPHTK GSQILMDLSH KAVKRQASFC NAITFSNRPV
110 120 130 140 150
LIYEQVRLKI TKKQCCWSGA LRLGFTSKDP SRIHPDSLPK YACPDLVSQS
160 170 180 190 200
GFWAKALPEE FANEGNIIAF WVDKKGRVFY RINESAAMLF FSGVRTVDPL
210 220 230 240 250
WALVDVYGLT RGVQLLDSEL VLPDCLRPRS FTALRRPSLR CEADEARLSV
260 270 280 290 300
SLCDLNVPGA DGDDGAPPAG CPIPQNSLNS QHSRALPAQL DGDLRFHALR
310 320 330 340 350
AGAHVRILDE QTVARLEHGR DERALVFTSR PVRVAETIFI KVTRSGGGRA
360 370 380 390 400
GALSFGVTTC DPGTLRPADL PFSPEALVDR KEFWAVCRVP GPLHSGDILG
410 420 430 440 450
LVVNADGELH LSHNGAAAGM QLCVDASQPL WMLFSLHGAI TQVRILGSTI
460 470 480 490 500
MTERGGPSLP CSPASTPTSP SALGIRLSDP LLSTCGSGPL GGSAGGTAPN
510 520 530 540 550
SPVSLPESPV TPGLGQWSDE CTICYEHAVD TVIYTCGHMC LCYSCGLRLK
560 570
KALHACCPIC RRPIKDIIKT YRSS
Length:574
Mass (Da):61,778
Last modified:December 1, 2001 - v1
Checksum:i0FC9A76509650709
GO
Isoform 2 (identifier: Q923S6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MGNNFSSVSSLQRGNPSRASRGHPQNLK → MGGQITRNTIH

Show »
Length:557
Mass (Da):59,978
Checksum:iC576111E30E0EC14
GO
Isoform 3 (identifier: Q923S6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-218: DS → GM
     219-574: Missing.

Show »
Length:218
Mass (Da):24,115
Checksum:i948A71AB2DE552FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197 – 199VDP → ADR (Ref. 3) Curated3
Sequence conflicti197 – 199VDP → ADR (Ref. 4) Curated3
Sequence conflicti301 – 302AG → RR (Ref. 3) Curated2
Sequence conflicti301 – 302AG → RR (Ref. 4) Curated2
Sequence conflicti316L → V (Ref. 3) Curated1
Sequence conflicti316L → V (Ref. 4) Curated1
Sequence conflicti332V → L (Ref. 3) Curated1
Sequence conflicti332V → L (Ref. 4) Curated1
Sequence conflicti333R → S in AAK97495 (PubMed:11585928).Curated1
Sequence conflicti348 – 350GRA → ARP (Ref. 3) Curated3
Sequence conflicti348 – 350GRA → ARP (Ref. 4) Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0131521 – 28MGNNF…PQNLK → MGGQITRNTIH in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_013153217 – 218DS → GM in isoform 3. 1 Publication2
Alternative sequenceiVSP_013154219 – 574Missing in isoform 3. 1 PublicationAdd BLAST356

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF401228 mRNA Translation: AAK97495.1
AF400063 mRNA Translation: AAK84420.1
Y15160 mRNA Translation: CAB65238.1
AJ271919
, AJ271920, AJ271921, AJ271922, AJ271923 Genomic DNA Translation: CAC88133.1
AK010787 mRNA Translation: BAB27183.1
BC058386 mRNA Translation: AAH58386.1
CCDSiCCDS29890.1 [Q923S6-1]
CCDS50463.1 [Q923S6-2]
RefSeqiNP_001156952.1, NM_001163480.1 [Q923S6-2]
NP_067335.4, NM_021360.4 [Q923S6-1]
UniGeneiMm.313651

Genome annotation databases

EnsembliENSMUST00000111807; ENSMUSP00000107438; ENSMUSG00000006435 [Q923S6-2]
ENSMUST00000111808; ENSMUSP00000107439; ENSMUSG00000006435 [Q923S6-1]
GeneIDi18011
KEGGimmu:18011
UCSCiuc008hut.1 mouse [Q923S6-3]
uc008huu.1 mouse [Q923S6-1]
uc008huv.2 mouse [Q923S6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF401228 mRNA Translation: AAK97495.1
AF400063 mRNA Translation: AAK84420.1
Y15160 mRNA Translation: CAB65238.1
AJ271919
, AJ271920, AJ271921, AJ271922, AJ271923 Genomic DNA Translation: CAC88133.1
AK010787 mRNA Translation: BAB27183.1
BC058386 mRNA Translation: AAH58386.1
CCDSiCCDS29890.1 [Q923S6-1]
CCDS50463.1 [Q923S6-2]
RefSeqiNP_001156952.1, NM_001163480.1 [Q923S6-2]
NP_067335.4, NM_021360.4 [Q923S6-1]
UniGeneiMm.313651

3D structure databases

ProteinModelPortaliQ923S6
SMRiQ923S6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201732, 4 interactors
IntActiQ923S6, 7 interactors
STRINGi10090.ENSMUSP00000107439

PTM databases

iPTMnetiQ923S6
PhosphoSitePlusiQ923S6

Proteomic databases

PaxDbiQ923S6
PRIDEiQ923S6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111807; ENSMUSP00000107438; ENSMUSG00000006435 [Q923S6-2]
ENSMUST00000111808; ENSMUSP00000107439; ENSMUSG00000006435 [Q923S6-1]
GeneIDi18011
KEGGimmu:18011
UCSCiuc008hut.1 mouse [Q923S6-3]
uc008huu.1 mouse [Q923S6-1]
uc008huv.2 mouse [Q923S6-2]

Organism-specific databases

CTDi18011
MGIiMGI:1334263 Neurl1a

Phylogenomic databases

eggNOGiKOG4172 Eukaryota
KOG4625 Eukaryota
ENOG410ZMNG LUCA
GeneTreeiENSGT00530000063117
HOGENOMiHOG000233742
InParanoidiQ923S6
KOiK01931
OMAiCPPVLPG
OrthoDBiEOG091G03VN
PhylomeDBiQ923S6
TreeFamiTF314368

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

ChiTaRSiNeurl1a mouse
PROiPR:Q923S6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006435 Expressed in 178 organ(s), highest expression level in gastrocnemius
CleanExiMM_NEURL
GenevisibleiQ923S6 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR037962 Neuralized
IPR006573 NHR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12429 PTHR12429, 1 hit
PfamiView protein in Pfam
PF07177 Neuralized, 2 hits
SMARTiView protein in SMART
SM00588 NEUZ, 2 hits
PROSITEiView protein in PROSITE
PS51065 NHR, 2 hits
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNEUL1_MOUSE
AccessioniPrimary (citable) accession number: Q923S6
Secondary accession number(s): Q91ZT6, Q9CWF1, Q9QWF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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