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Protein

Splicing factor 3B subunit 5

Gene

Sf3b5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 5
Short name:
SF3b5
Alternative name(s):
Pre-mRNA-splicing factor SF3b 10 kDa subunit
Gene namesi
Name:Sf3b5
Synonyms:Sf3b10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1913375 Sf3b5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002207582 – 86Splicing factor 3B subunit 5Add BLAST85

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei9PhosphoserineBy similarity1
Modified residuei17N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ923D4
MaxQBiQ923D4
PaxDbiQ923D4
PeptideAtlasiQ923D4
PRIDEiQ923D4

PTM databases

PhosphoSitePlusiQ923D4
SwissPalmiQ923D4

Expressioni

Gene expression databases

BgeeiENSMUSG00000078348 Expressed in 288 organ(s), highest expression level in primary oocyte
CleanExiMM_SF3B5
GenevisibleiQ923D4 MM

Interactioni

Subunit structurei

Component of the spliceosome B complex. Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Within the SF3B complex interacts directly with SF3B1 (via HEAT domain) and SF3B3. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei5Interaction with RNABy similarity1
Sitei20Interaction with RNABy similarity1

Protein-protein interaction databases

IntActiQ923D4, 3 interactors
MINTiQ923D4
STRINGi10090.ENSMUSP00000100771

Structurei

3D structure databases

SMRiQ923D4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 76Interaction with SF3B1 and SF3B3By similarityAdd BLAST62

Sequence similaritiesi

Belongs to the SF3B5 family.Curated

Phylogenomic databases

eggNOGiKOG3485 Eukaryota
ENOG4111NTV LUCA
GeneTreeiENSGT00390000013215
HOGENOMiHOG000210519
HOVERGENiHBG054589
InParanoidiQ923D4
KOiK12832
OMAiQSKYVGT
OrthoDBiEOG091G0ZLI
PhylomeDBiQ923D4
TreeFamiTF300117

Family and domain databases

InterProiView protein in InterPro
IPR009846 SF3b5/RDS3-10
IPR017089 Splicing_factor_3B_subunit_5
PANTHERiPTHR20978 PTHR20978, 1 hit
PfamiView protein in Pfam
PF07189 SF3b10, 1 hit
PIRSFiPIRSF037010 Splicing_factor_3B_subunit_5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q923D4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL
60 70 80
NYFAIAENES KARVRFNLME KMLQPSGPPA DKPEEN
Length:86
Mass (Da):10,119
Last modified:December 1, 2001 - v1
Checksum:iB8A21DBA9F548E8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003475 mRNA Translation: BAC25037.1
AK009874 mRNA Translation: BAC25275.1
BC006603 mRNA Translation: AAH06603.1
CCDSiCCDS35843.1
RefSeqiNP_780311.2, NM_175102.4
UniGeneiMm.29743
Mm.402225

Genome annotation databases

EnsembliENSMUST00000105139; ENSMUSP00000100771; ENSMUSG00000078348
GeneIDi66125
KEGGimmu:66125
UCSCiuc007eki.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003475 mRNA Translation: BAC25037.1
AK009874 mRNA Translation: BAC25275.1
BC006603 mRNA Translation: AAH06603.1
CCDSiCCDS35843.1
RefSeqiNP_780311.2, NM_175102.4
UniGeneiMm.29743
Mm.402225

3D structure databases

SMRiQ923D4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ923D4, 3 interactors
MINTiQ923D4
STRINGi10090.ENSMUSP00000100771

PTM databases

PhosphoSitePlusiQ923D4
SwissPalmiQ923D4

Proteomic databases

EPDiQ923D4
MaxQBiQ923D4
PaxDbiQ923D4
PeptideAtlasiQ923D4
PRIDEiQ923D4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105139; ENSMUSP00000100771; ENSMUSG00000078348
GeneIDi66125
KEGGimmu:66125
UCSCiuc007eki.2 mouse

Organism-specific databases

CTDi83443
MGIiMGI:1913375 Sf3b5

Phylogenomic databases

eggNOGiKOG3485 Eukaryota
ENOG4111NTV LUCA
GeneTreeiENSGT00390000013215
HOGENOMiHOG000210519
HOVERGENiHBG054589
InParanoidiQ923D4
KOiK12832
OMAiQSKYVGT
OrthoDBiEOG091G0ZLI
PhylomeDBiQ923D4
TreeFamiTF300117

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Miscellaneous databases

PROiPR:Q923D4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078348 Expressed in 288 organ(s), highest expression level in primary oocyte
CleanExiMM_SF3B5
GenevisibleiQ923D4 MM

Family and domain databases

InterProiView protein in InterPro
IPR009846 SF3b5/RDS3-10
IPR017089 Splicing_factor_3B_subunit_5
PANTHERiPTHR20978 PTHR20978, 1 hit
PfamiView protein in Pfam
PF07189 SF3b10, 1 hit
PIRSFiPIRSF037010 Splicing_factor_3B_subunit_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSF3B5_MOUSE
AccessioniPrimary (citable) accession number: Q923D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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